Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Detection of Fish Rhabdoviruses using a Diagnostic Fish Rhabdovirus DNA Chip

  • Kim, Young-Ju (Department of Microbiology, Pukyong National University) ;
  • Lee, Myung-Suk (Department of Microbiology, Pukyong National University)
  • Published : 2005.09.01
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Abstract

We tested the in vivo ability of a DNA chip to detect virus-specific genes from virus-infected olive flounder Paralichthys olivaceus and rainbow trout Oncorhynchus mykiss. Target cDNA was obtained from total RNA of virus infected cell lines by reverse transcription (RT) and was labeled with fluorescent dye (Cy5-dUTP). The results show the successful detection of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) genes in the virus-infected fishes.

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References

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