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http://dx.doi.org/10.5657/fas.2005.8.3.185

Detection of Fish Rhabdoviruses using a Diagnostic Fish Rhabdovirus DNA Chip  

Kim, Young-Ju (Department of Microbiology, Pukyong National University)
Lee, Myung-Suk (Department of Microbiology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.8, no.3, 2005 , pp. 185-187 More about this Journal
Abstract
We tested the in vivo ability of a DNA chip to detect virus-specific genes from virus-infected olive flounder Paralichthys olivaceus and rainbow trout Oncorhynchus mykiss. Target cDNA was obtained from total RNA of virus infected cell lines by reverse transcription (RT) and was labeled with fluorescent dye (Cy5-dUTP). The results show the successful detection of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) genes in the virus-infected fishes.
Keywords
Rhabdovirus; In vivo detection; DNA chip; Paralichthys olivaceus; Oncorhynchus mykiss;
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1 Dixon, P.F. and B. J. Hill. 1984. Rapid detection of fish rhabdoviruses by the enzyme-linked immunosorbent assay (ELISA). Aquaculture, 42, 1-12   DOI   ScienceOn
2 Kim, S.J. 1999. Comparison of infectious hemato-poietic necrosis virus detection methods. MS thesis, Pukyong National University, Korea, pp. 1-36
3 Kim, S.M. 2003. Infection of viral hemorrhagic septicemia virus (VHSV) in cultured olive flounder, Paralichtyys olivaceus. Ph.D. thesis, Pukyong National University, Korea, pp. 1-21
4 Kim, Y.J., J.H. Kang, S.M. Kim, S.I. Park, S.B. Kim and M.S. Lee. 2005. Optimization of a diagnostic DNA chip for fish rhabdovirus. J. Fish. Sci. Technol., 8, 122-127   DOI   ScienceOn
5 Rimstad, E., R. Krona, E. Hornes, Q. Olsvik and B. Hyllseth. 1990. Detection of infectious pancreas-tic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe. Vet. Microbiol., 23, 211-219   DOI   ScienceOn
6 Schena, M. 2003. Microarray Analysis. Wiley-Liss Book Co., New Jersey, pp. 1-25
7 Tran, P.H., D.A. Peiffer, Y. Shin, L.M. Meek, J.P. Brody and K.W. Cho. 2002. Microarray optimization:increasing spot accuracy and automated identification of true microarray signals. Nucl. Acids Res., e54
8 Vernet, G.. 2002. DNA chip technology and infectious diseases. Virus Res., 82, 65-71
9 Willams, K., S. Blake, A. Sweeney, J.T. Singerand and B.L. Nicholson. 1999. Multiplex reverse transcriptase PCR assay for simultaneous detec-tion of three fish viruses. J. Clin. Microbiol., 37, 4139-4141
10 Koutna, M., T. Vesely, I. Psikal and J. hulova. 2003. Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR. Dis. Aquat. Org., 55, 229-235   DOI
11 Hus, Y.L. and J.C. Leong. 1985. A comparison of detection methods for infectious hematopoietic necrosis virus. J. Fish Dis., 8, 1-12   DOI
12 Bowtell, D. and J. Sambrook. 2003. DNA Microarrays, Cold Spring Habor Laboratory Press, New York, pp. 79-256