• KSBB Journal
• /
• v.24 no.2
• /
• pp.182-188
• /
• 2009

Lactobacillus spp., primary members of probiotics, have significant benefits for health and well-being of human. In this study Lactobacillus strains representing six species (L. paracasei KLB58, L. fermentum MS79 and KLB282, L. plantarum KLB213, L. gasseri KLB238, and L. reuteri KLB270) isolated from Korean adults were electrotransformed with plasmid pNCKH104. To determine optimal electrotransformation conditions, various conditions including cell wall weakening agent, electroporation buffer, electric field strength and time constant were tested for each strain. Overall, high transformation efficiency of approximately 2.5 ${\times}$ $10^3$ ${\sim}$ 5.5 ${\times}$ $10^4$ CFU/${\mu}g$ DNA was obtained where conditions of 0.5 M sucrose electroporation buffer, 1.8 kV pulse voltage and 5 ms time constant were applied. The common conditions developed in this study will make transformation of various Lactobacillus spp. easier than previous procedures.

• Spatial Information Research
• /
• v.23 no.2
• /
• pp.21-29
• /
• 2015

Recently, according to the increasingly populated data, many media and organizations focus on big data, data visualization, information visualization and infographics. Domestically, Chosun.com and Hankyoreh online have improved on the data visualization field and internationally, the Guardian, Wall Street Journal, and New York Times are the leading companies on that area. Until now, many people have recognized infographics as a design-oriented product in Korea. However, one of significant data visualization programs, Tableau Public, can visualize data more efficiently. In this paper, Data Visualization Methods Quadrant for Policy Making is defined, and data analysis and producing infographics are executed. As used data, World Bank open source was adopted and using the number of passenger cars per 1,000 people, two analysis results are extracted. First, in high income group, the more GNI per capita, the lesser Slope is represented and in mid income group, the more GNI per capita positively affects to Slope. Second, in the global finance crisis, the car ownership rate was about 1.7 times than the usual state in the global economy. Through the case study, this paper suggests that the direction of producing infographics should be changed from design-oriented to data-oriented. Moreover, the data-oriented infographics should be propagated as means of scientific research and policy making.

• Proceedings of the KOR-BRONCHOESO Conference
• /
• 1981.05a
• /
• pp.8.5-9
• /
• 1981

Foreign body in the esophagus is not uncommon in the otolaryngological field and esophageal perforation followed by mediastinitis is one of the most serious complications. Authors had experienced such case developed in 69 year old female patient. This woman swallowed a piece of sharp glass accidentally. Severe pain and swelling around the neck developed after ingestion. Marked subcutaneous emphysema was noted on first examination. By esophagoscopy, longitudinal laceration at right lateral wall of the cervical esophagus was noted and a lot of food debries were removed through this perforation, but foreign body could not be found. On third hospital day, patient complained chest pain and dyspnea. Mediastinal widening was noted on chest P-A. Tracheostomy was performed on next day and neck swelling decreased much. In spite of massive antibiotics, mediastinal abscess was developed and external drainage was performed on 15th hospital day. She was discharged on 38th hospital day with marked improvement and recovered completely on follow-up study. Still, we do not know where the foreign body is located.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.16 no.8
• /
• pp.1552-1565
• /
• 1992

Three dimensional numerical calculations were carried out for two different combustion chambers with the offset valve in order to investigate the swirl and the squish effects on the flow fields. The modified K-.epsilon. turbulence model considering the change of the density under the condition of the rapid compression and expansion of the pistion was used. During the compression process, it was found that the squish flow which controls the subsequent combustion process was produced due to the piston bowl in the bowl piston type combustion chambers but not for the flat piston type. The swirl velocity close to the solid body rotation was maintained in the flat piston type combustion chambers, but for the bowl piston type a resulting from the change of the solid body rotation was generated in the radial-circumferential plane. For the swirl ratio effect, as the swirl ratio increases, it was found that a large and strong vortex was generated in the radial-circumferential plane of bowl piston type combustion chambers because of the strong inward flows from the combustion chamber wall. These computational results were compared with the results of LDA measurement.

• The Journal of Engineering Geology
• /
• v.12 no.2
• /
• pp.167-178
• /
• 2002

Properties of discontinuity for Seoul Granite in northeastern part of Seoul City were analyzed by dividing structural domains into Surak and Bulam Mtn. areas. Important parameters measured among several engineering properties of a rock during tunnel excavation and road construction are as follows: 1) Orientation of joint, 2) joint spacing, 3) joint density, and 4) uniaxial compressive strength. Orientation, spacing, and density of joints can be directly measured during field investigation using scanline survey, circle-inventory method, and window survey. Uniaxial compressive strength of the rock was calculated by a simple correlation equation although it is originally necessary to prepare core samples in measuring it. Major orientations of joints measured from both areas are 3 sets of joints with different orientations. In other words, they are 2 sets of orthogonal joint and 1 set of sheet joint that is dipping at low angle, and have very similar orientations in both areas. Joint densities in both areas range from 0.039 and 0.066/cm, and average joint length are between 1.30 and 4.52m. Average joint spacing also has values from 10.3cm up to 59.6cm, and shows significant difference along specific orientation of scanlines measured. Values of uniaxial compressive strength calculated on the basis of Schmidt hammer rebound values range from 217 to 335 MPa, which indicates very strong rock type by classification of wall strength.

• The Korean Journal of Pharmacology
• /
• v.31 no.2
• /
• pp.195-206
• /
• 1995

The study was undertaken to examine the possibility of the involvement of $K^+$ channels in the mechanism of relaxant-action of imipramine on the isolated canine detrusor muscle strips. Canine urinary bladder were isolated, and smooth muscle strips of 15 mm long and 2 mm wide from the mid-portion of anterior wall were made in the Tyrode solution of $0{\sim}4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with $95%\;O_2/5%CO_2\;at\;37^{\circ}C$. RP 52891, a non-specific $K^+$ channel opener, concentration-dependently suppressed the spontaneous phasic contractions of the detrusor strips. Imipramine, a tricyclic antidepressant, also reduced the spontaneous contractions in a concentration-dependent manner. RP 52891 was more potent than imipramine(p<0.05), and Imipramine was more efficient than RP 52891(p<0.05).Procaine, a voltage-dependent $K^+$ channel blocker, glibenclamide, an ATP-dependent $K^+$ channel blocker, and apamin, a calcium-dependent $K^+$ channel blocker antagonized the relaxant effect of RP 52891, but not of imipramine. Imipramine reduced the electric field stimulation (EFS) -induced contractions concentration-dependently. None of the $K^+$ channel blockers employed for this study, procaine, glibenclamide or apamin antagonized the inhibitory action of imipramine on the EFS-induced contraction. These results suggest that in canine detrusor, the $K^+$ channels of the characteristics of voltage-dependent, ATP-dependent and/or calcium-dependent are exist, and the inhibitory action of imipramine on the contractility of the detrusor is independent from the $K^+$ channels.

• Land and Housing Review
• /
• v.2 no.4
• /
• pp.397-406
• /
• 2011

This research aims to develop various infill-systems for practically applying to long-life housing with 100-year durability and flexibility as a strategy for realizing sustainable residential environment. Especially, the developed infill-systems & technologies were concentrated to the mock-up house applied with the standard model of long-life housing, and their in-situ applicability by a phase, experts' consultation, and questionnaire survey were also evaluated. This research was executed to divide by 3 phases. In the First phase infill-systems for a housing unit of $84m^2$ exclusive use area as well as a support of whole structure and infill-systems including dry wall, and windows were constructed, and their in-situ applicability were evaluated. In the Second phase vertical integration of upper and lower housing units of $50m^2$ and $40m^2$ exclusive use area was investigated the first in domestic for 3 housing units of $50m^2$ exclusive use area. In addition, various infill-systems including the horizontal expansion of $50m^2$ and $10m^2$ exclusive use areas were also constructed and evaluated on their in-situ applicability. In the third phase diverse performance tests and field investigation for in-situ verifying of dry Ondol, developed this research, were executed to improve their practicability. Inner dry walls were taken apart, moved and re-constructed for verifying their practicability to investigate 3R-Reduce, Reuse and Recycle-realization too.

• Korean Journal of Organic Agriculture
• /
• v.22 no.4
• /
• pp.855-868
• /
• 2014

Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test.

• Applied Chemistry for Engineering
• /
• v.16 no.6
• /
• pp.794-799
• /
• 2005

With the quantitative growth of the petroleum industry, the pipe facilities that connect each process increased significantly and the corresponding maintenance and repair costs of the pipe facilities increased as well. The diagnosis techniques to check a pipe efficiency while in operation are few in Korea, but in the advanced countries the pipe diagnosis using gramma-ray source was on-going research since 1960's. In this study, field experiments were performed to analyze the reasons for abnormal operation of the pipe connected to a distillation tower, and the degree of abnormality was estimated using a sealed gamma-ray source ($^{137}Cs$). Gamma radiation counts were measured by a detector (NaI) positioned outside the pipe-wall diametrically opposite to the gamma source. The results showed that a gas zone section's distribution pattern was different from the pattern of nearby fluid in a pipe. Th diagnosis technique using a gamma radiation source was proved to be an effective and reliable method, offering the information on the fluid distribution in pipe.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.24 no.6
• /
• pp.495-512
• /
• 2022

Due to the concentration of population in the city center, the aboveground structures are saturated, and the development of underground structures becomes important. In addition, it is necessary to apply the reinforcement construction method for the pillar part of the adjacent tunnel that can secure stability, economy, and workability to the site. In this study, the tunnel pillar reinforcement method using prestress and grouting was reviewed. There are various reinforcement methods that can compensate for the problems of the side tunnel, but as the tunnel pillar construction method using prestress and grouting is judged to be excellent in field applicability, stability, and economic feasibility, it is necessary to review the theoretical and numerical analysis of the actual behavior mechanism. Therefore, a scaled-down model experiment was conducted. The reduced model experiment was divided into PC stranded wire + steel pipe reinforcement grouting + prestress (Case 1), PC strand + steel pipe reinforcement grouting (Case 2), and no reinforcement (Case 3), and the displacement of the pillar and the earth pressure applied to the wall were measured. Through experiments, it was confirmed that the PC stranded wire + steel pipe reinforcement grouting + prestress method is the most excellent reinforcement method among various construction methods. It was judged that it could be derived.