• Title/Summary/Keyword: Fecal Coliform

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Survey on Bacteriological Contamination of Restaurants in Seoul Area (서울지역 대중음식점의 위생상태에 관한 미생물학적 조사연구 - 물수건, 냉면육수, 엽차를 중심으로 -)

  • Shin Jung Sik;Park Sang Huun
    • Journal of environmental and Sanitary engineering
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    • v.1 no.1 s.1
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    • pp.41-46
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    • 1986
  • This study was performed to investigate the sanitation of restaurants in Seoul Area. The subjects were 153 wet towels, 64 cold noodle soup and 190 barely tea. The results were as follows: In wet towels: The detected rate of standard plate counts was $86.9\%$ (133 samples) and average count was $1.8\times10^3/g$ $the detected rate of coliform was $37.9\%$ (58 samples) and average count by MPN method was $2.0\times10^3/100g$, the detected rate of fecal coliform was $15.7\%$ (24 samples) and average count by MPN method was $3.2\times10/100g$. In cold noodle soups: The detected rate of standard plate counts was $100\%$ (64 samples) and average count was $9.4\times10^5/ml$, the detected rate of coliform was $75\%$ (48 samples) and average count by MPN method was $6.0\times10^5/100ml$, the detected rate of fecal coliform was $51.6\%$ (33 samples) and average count by MPN method was $3.4\times10^3/100ml$. In barely tea: The detected rate of standard plate counts was $87.4\%$(166 samples) and average count was $5.8\times10^3/ml$the detected rate of coliform was $66.3\%$ (126 samples) and average count by MPN method was $3.9\times10^3/100ml$, the detected rate of fecal coliform was $32.6\%$ (62 samples) and average count was by MPN method was $4.7\times10/100ml$.

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Evaluation of Microbiological Safety of Commercially Salt-fermented Fishery Products by Raw Materials (젓갈류의 원료에 따른 세균학적 안전성 평가)

  • Shim, Kil Bo;Park, Kunbawui;Yoon, Na-Young;An, Byoung Kyu;In, Jung Jin;Han, Hyeong Gu;Lee, Woo Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.6
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    • pp.1045-1051
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    • 2021
  • Eighty-nine different types of commercially salt-fermented fishery products comprising various raw materials were analyzed for total aerobic bacteria, number of coliform bacteria, fecal coliform, and Escherichia coli. The food-poisoning bacterial content of the samples was investigated using next-generation sequencing. The mean mass of total aerobic bacteria in Jeotgal was 6-1.8×109 CFU/g, and that in Aekjeot and Sikhae was 4-2.2×105 CFU/mL and 1.9×105-8.4×108 CFU/g, respectively. Coliform bacteria were detected in 9 (28.1%) of 32 Jeotgal samples; 15 (46.8%) of 32 seasonal Jeotgal samples; and in 5 (55.5%) of 9 Sikhae samples. Fecal coliform and E. coli were not detected in 86 of the 89 samples. Yersinia enterocolitica was detected only in Galchi jeot (salt-fermented hairtail) (1 type) and not in other Jeotgal samples. These results contribute to our knowledge regarding the bacterial stability of salt-fermented fishery products.

A Bacteriological Study on the Indoor-Shoes, Desk and Bag of Student의s (실내화, 책상, 책가방의 위생학적 고찰)

  • Kong, Dong
    • Journal of Environmental Health Sciences
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    • v.4 no.1
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    • pp.26-33
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    • 1977
  • A bacteriological research of the school desks, bags and indoor-shoes was made from Oct. 2, 197 through May 28, 1976. The research included one girl's middle school, two boy's middle schools, two boy's high schools and 15 girl's high schools, alltogether 244 students from 28 schools, The research was analyzed and the results are as follows: 1) The research showed that 95% of the indoor-shoes materials were of cloth. Only 13% of the students carried the indoor-shoes in shoes sacks and 84.8% of them kept the shoes in schoolbags with books and lunchbox. 2) Only 5% of them cleaned their shoes every week and 79% cleaned every six. 3) 49.3% of the students kept the indoor-shoes in the school bag with other contents at home. 4) 88% of the bag was made of vinyl and 24.8% of the students carried their lunch in their bag without lunch box. 5) The research showed that 79.1% of the students never cleaned their bags. 6) In using the desk drawer, 58.2% of the students put their out-door shoes into the drawer, 18.9% of them kept their lunch in it, 11.9% used it keeping books alone and 11% keeping school bags with books. 7) 30.4% of the students had an experience of having cleaned their desks and 69.6% were unconcerned wirh desk-cleaning. 8) 34.4% of the indoor-shoes using school showed fecal coliform positive, 40.2% showed non-fecal coliform positive and 25.4% showed no reaction to IMViC. 9) The 34.4% of the fecal coliform positive showing schools, equivalant to 77 students, was composed of zero percents of girl's middle school, 18.2% of girl's high school, 36.4% of boy's middle school and 45.4% of boy's high school. 10) Among the 77 students, whose shoes showed fecal coliform positive, only 6.5% of them cleaned their shoes once in a week or a month and 62.3% of them cleaned them once in a six month. 11) The fccal coliform positive rate was higher in the cloth shoes materials than the vinyl materials. 12) The concrete-bottomed building higher positive rate (40.2%) than the wood-bottomed building (7.5%). 13) Even among the student's who frequently cleaned their desk drawer, 2.3% were in the positive, and among those who were unconcerned with drawer cleaning, 48.9% were involved in the positive. 14) In the fecal coliform positive rate of bags, 38.5% of the positive rate belonged to using indoor shoes while cleaning their class bottom with water, 53.8% belonged to using outdoor shoes while cleaning their class bottom with water, 7.7% belonged to using outdoor shoes while cleaning their class with oil.

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Coliform Pollution Status of Nakdong River and Tributaries (낙동강수계 본류와 유입지천의 대장균군 오염도)

  • Lee, Hae-Jin;Park, Hae-Kyung;Lee, Jae Hak;Park, A Reum;Cheon, Se-Uk
    • Journal of Korean Society on Water Environment
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    • v.32 no.3
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    • pp.271-280
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    • 2016
  • The aim of this study was to analyze spatial and temporal patterns of bacterial pollution levels and the relationship between bacterial pollutants and environmental parameters at the main stream and tributaries of Nakdong River. Water quality data including total coliform and fecal coliform were compiled from a total of 50 monitoring sites (30 at the main stream and 20 at the tributaries) along with rainfall and discharge data for three consecutive years from 2012 to 2014. During the study periods, the geometric mean values of total coliforms and fecal coliforms in the main stream were 74 (22~465) CFU/100 mL and 8 (3~42) CFU/100 mL, respectively. The geometric mean values of total coliforms and fecal coliforms in the tributaries were 275 (36~5,145) CFU/100 mL and 6 (1~1,352) CFU/100 mL, respectively. High concentrations of fecal coliforms were observed at Gumi (M 10), Hyeonpung (M 19), Hapcheon (M 23), and Namji (M 25) in the main stream, whereas Gamcheon (T 6), Bakcheon (T 7), Geumho-gang (T 8), and Gyeseongcheon (T 16) were identified as pollution hot spots in the tributaries. Although bacterial pollution levels showed complex behavior across monitoring sites and time, the highest coliform concentrations were routinely observed in the monsoon season between July and September of each year, indicating that the pollution levels were strongly dependent on precipitation in addition to other physiochemical parameters. Statistically significant correlations were found between fecal coliform concentrations and precipitation (r=0.403, p<0.01), followed by SS (r=0.425, p<0.01), nutrient TP (r=0.388, p<0.01), organic matter COD (r=0.322, p<0.01), and PO4-P (r=0.317, p<0.01) in the main stream in the order of correlation coefficient from high to low.

Evaluation of the Bacteriological Safety for the Shellfish Growing Area in Hansan.Geojeman, Korea (한산.거제만해역 패류양식장에 대한 세균학적 위생안전성 평가)

  • Ha, Kwang-Soo;Shim, Kil-Bo;Yoo, Hyun-Duk;Kim, Ji-Hoe;Lee, Tae-Seek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.5
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    • pp.449-455
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    • 2009
  • In Hansan Geojeman area, 2,050 ha of shellfish growing area has been designated as shellfish production area for export. The main shellfish species from the designated area is oysters. For the sanitary management of the designated area established in Hansan Geojeman area, bacteriological examination of sea water and shellfish at the sampling stations inside and outside of the designated area were performed from January 2006 to December 2008. The range of fecal coliform of 756 sea water samples at 21 stations located in the designated area were <1.8~>1,600 MPN/100mL. And the range of geometric mean and the estimated 90th percentile of fecal coliform were 1.8~2.9 and 2.7~15.8 MPN/100mL, respectively. Sanitary conditions of the current designated area in Hansan Geojeman meets the required standards of the Fisheries Product Quality Control and National Shellfish Sanitation Program (NSSP, USA) criteria for the approved area. Also, the sanitary status of the shellfish harvested from the designated area met the Korean Shellfish Sanitation Program (KSSP) fecal coliform criterion (<230 MPN/100g). And the human pathogen such as Salmonella spp. and Shigella spp. were not detected from the examined shellfish samples.

An Analysis on Removal Effect of Biological Contaminants in the Process of Municipal Sewage Treatment System - On the Seoul Cheonggye Cheon Sewage Treatment Plant (도시하수 처리에 의한 미생물 오염의 제거효과에 관한 조사연구(I) -청계천 하수처리장을 중심으로-)

  • Yu Byong Tae;Chung Yong
    • Journal of environmental and Sanitary engineering
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    • v.3 no.1 s.4
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    • pp.27-39
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    • 1988
  • This investigation was carried out to evaluate the removal effect of biological contaminants for the municipal sewage treatment process at Cheonggye Cheon terminal plant which in the first plant for municipal sewage treatment in Seoul area. It was conducted in raw influent, primary treatment water and secondary treatment water from September, 1986 to July, 1987. The results were as follow; 1, The primary treatment could eliminate microbials for $65.38\%$ of total bacteria, $64.35\%$ of total coliform, $62.16\%$ of fecal coliform $69.48\%$ of pseudomonas and $64.70\%$ of fecal streptococci in averages for a year respectively. 2. The secondary treatment could eliminate microbials for $97.50\%$ of total bacteria, $97.30\%$of total coliform, $95.95\%$ of fecal coliform, $97.00\%$ of pseudomonas and $96.53\%$ of fecal streptococci in average for a year respectively. 3. In the detect rate of pathogenic agent, salmonella spp was decreased $12.5\%$ to $4.2\%$ in primary treatment and it was not detected in secondary treatment, shigella spp was detected $4.2\%$ in influent water but it was not detected in primary and secondary treatment. 4. In the seasonal variation of treatment effect, the removal of summer was the highest, and the removal of all item in winter was lower than the other seasons. 5. There was significant correlation between water temperature and microbal all items (P<0.05) $NH_3-N$ and Microbal items (P< 0.01) at raw water.

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Impact of Rainfall Events on the Bacteriological Water Quality of the Shellfish Growing Area in Korea (패류 양식장의 세균학적 수질에 미치는 강우의 영향)

  • Lee, Tae-Seek;Oh, Eun-Gyoung;Yu, Hyeun-Duck;Ha, Kwang-Soo;Yu, Hong-Sik;Byun, Han-Seok;Kim, Ji-Hoe
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.5
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    • pp.406-414
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    • 2010
  • The impact of rainfall events on the sanitary indicator bacteria density of the shellfish-growing waters in Geoje Bay and Jaran Bay in Korea was investigated. The shellfish-growing area in Geoje Bay, which is a nearly closed basin, was not affected significantly, except near the stream mouth after 11.5 mm of rainfall in 1 day. However, most of the shellfish-growing water in the bay was polluted by fecal coliform bacteria after rain as heavy as 43.0 mm, and the levels of fecal indicator bacteria in some of the sea near the coast did not recover completely until 24 hours after the rainfall. By contrast, in Jaran Bay, which has no significant pollution source in the drainage area, although 9.3-490 MPN/100 mL of fecal coliform bacteria were detected near the stream mouth after rainfall of 33.5 and 81.0 mm, a very low level of the indicator bacteria was detected in the designated shellfish-growing area. During the investigation, the correlations between the sanitary indicator bacteria density and physical parameters, such as salinity and turbidity, were evaluated. Both the total coliform and fecal coliform densities were inversely correlated with salinity. Turbidity was positively correlated with the indicator bacteria density. The survey results suggest that for more efficient management of the shellfish-growing areas located in coastal areas, such as shellfish harvesting after rainfall, a detailed investigation of the effects of rainfall on the bacterial water quality in each growing area is needed.

Evaluation of the Influence of Inland Pollution Sources on Shellfish Growing Areas after Rainfall Events in Geoje Bay, Korea (강우에 따른 거제만해역 육상오염원의 영향평가)

  • Ha, Kwang-Soo;Yoo, Hyun-Duk;Shim, Kil-Bo;Kim, Ji-Hoe;Lee, Tae-Seek;Kim, Poong-Ho;Ju, Ja-Yeon;Lee, Hee-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.612-621
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    • 2011
  • The influences of inland pollution sources because of rainfall events on the bacteriological water quality in Geoje Bay, a major shellfish production area in Korea, were investigated. The sanitary status of sea water and shellfish after rainfall events was also evaluated. The flow rates of 13 streams around Geoje Bay showed 6 to 7-fold increases after 15 to 21 mm of rainfall. Peak pollution was observed in the Naegan Stream, the Gandeok Stream and the Seojeong Stream. The calculated impact area of inland pollution sources was 3.1 $km^2$ immediately after 15 mm of rainfall and expanded to 3.5 $km^2$ after 24 hours. These calculations of impacted area matched results from fecal coliform analyses with sea water. The distance between the major pollution source in the bay (the Gandeok Stream) and the station with the worst bacteriological water quality immediately after 15 mm of rainfall, which was below the Korean standard, was 0.8 km in a straight line; this distance increased to 2.0 km after a period of 24 hours. The area impacted by inland pollution sources after a 15 mm rainfall event was wider than after a 21 mm rainfall. Although the flow rate from inland pollution sources was higher, the concentration of fecal coliform in the discharged water was lower after higher rainfall events. These observations corresponded with the results of fecal coliform analyses with sea water samples. According to the evaluation of the influences of inland pollution sources and fecal coliform analyses on sea water and shellfish samples in Geoje Bay, pollutants from inland sources did not reach the boundary line of the shellfish growing area after rainfall events of 15 or 22 mm. The bacteriological water quality of the shellfish growing area in Geoje Bay met the Korean standard and US NSSP requirements for approved shellfish growing areas.