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Composition, the Changes of Diastase Activity and Hydroxymethylfurfural Content During Storage of the Various Honey Samples (벌꿀의 조성(組成)과 저장중(貯藏中)의 Diastase 및 Hydroxymethylfurfural함량변화(含量變化))

  • Han, Jae-Gyeong;Kim, Kwan;Kim, Dong-Youn;Lee, Sang-Kyu
    • Korean Journal of Food Science and Technology
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    • v.17 no.3
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    • pp.155-162
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    • 1985
  • The sugers and free amino acids of honey(Western and Domestic honey) and the changes of diastase activity during storage at various temperatures and HMF were studied. The average content of sugers in the western honey was 40.20% of fructose, 32.43% of glucose and 1.0% of sucrose. The average ratio of fructose to glucose was 1.24. In the case of the domestic honey, average content of sugers was 37.37% of fructose, 31.92% of glucose and 0.74% of sucrose and the average ratio of fructose to glucose was 1.19. Of the 16 amino acids detected, proline was the most predominant free amino acid in all of the honey studied, representing 39.59 to 46.86% of total free amino acids. Diastase activity of honey during the storage at $50^{\circ}C$ was rapidly decreased, but it was notably changed during storage at low temperature ($4^{\circ}C$) and room temperature ($20^{\circ}C$). The accumlated content of HMF were 0.12 to 0.47mg% during storage at 80 days at $20^{\circ}C$ and 5.35 to 8.71mg% after 3 weeks storage at $50^{\circ}C$.

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Studies on the Nutritional Components of Purple Sweet Potato(Ipomoea batatas) (자색(紫色) 고구마의 영양성분에 관한 연구)

  • Kim, Sun-Young;Ryu, Chung-Hee
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.819-825
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    • 1995
  • Two sweet potato CV.(Ipomoea batatas) were examined, i.e. purple flesh sweet potato(PSP) and light yellow flesh sweet potato(LYSP) which varied in degree of sweetness. On a fresh weight basis, nitrogen free extract in cultivars ranged from $25.73{\sim}26.24%$ and PSP contained more crude fat than LYSP. Total amino acids of PSP and LYSP were 5676.57mg% and 4550.86mg%, respectively. Aspartic acid, serine, alanine and valine were the major components in sweet potatoes. Sulfur-containing amino acids are the first limiting amino acid in PSP. The major fatty acids in PSP and LYSP analyzed by GC were palmitic acid, linoleic acid. The content of the saturated fatty acid was less than that of the unsaturated fatty acid. Carbohydrate contents were $75.43{\sim}79.10%$ and neutral sugars contents were $67.22{\sim}64.85%$(dry wt). Two sweet potato CV. contained the most glucose of all neutral sugars. PSP contained 11.88% for uronic acid, 59.42% for starch. Free sugars of PSP(0.82%) was much less than that of LYSP(2.53%). The contents of thiamin, riboflavin and niacin were similar, and the ascorbic acid contents in PSP and LYSP were 63.4mg% and 48.7mg%(dry wt), respectively. Comparing the mineral content in PSP, K was the greatest element in concentration followed by mg, Ca, Na. The total dietary fiber(TDF) value was 13.43% in PSP, 9.79% in LYSP respectively. The ratio of soluble dietary fiber(SDF) content and insoluble dietary fiber(IDF) content to TDF content for PSP were 57.6%, 42.4%, respectively.

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Effect of Dendranthema indicum Extracts on Cell and DNA Damage Induced by Oxidative Stress (국화추출물이 산화적 스트레스에 의해 유발되는 세포와 DNA 손상에 미치는 영향)

  • Park, Young-Mi;Kim, Jee-In;Lee, Chang-Ho;Lim, Jae-Hwan;Seo, Eul-Won
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1698-1704
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    • 2011
  • In this study, we evaluated the protective effects of ethanol extracts from Dendranthema indicum on cell and DNA damages induced by oxidative stress. Antioxidant activities of D. indicum extracts are higher than scavenging activities of DPPH free radical and hydroxyl radical by 92.8% and 73.8%, respectively, and higher than ferrous iron chelating effects by 59.4%. D. indicum extracts showed a protective effect on oxidative cell damage by inhibiting lipid peroxidation by 90.3% in the control group, and inhibiting expression level of p21 protein by 79.6% for the control group. This means D. indicum extracts have a great protective effect against oxidative stress. DNA fragmentation inhibition in D. indicum extracts were 89.6% for the control group, which makes the movement of DNA tail reduced, and phosphorylation of H2AX was 20.2% of the radical experiment group. This means that D. indicum extracts effectively inhibit DNA fragmentation and H2AX phosphorylation. Taken together, we suggest that ethanol extract from D. indicum has a role as a useful chemopreventor against oxidative damage.

Optimization of Production of Trehaolse from Maltose using Recombinant Trehalose Synthase from Thermus caldophilus GK24 (재조합 트레할로스 합성효소에 의한 맥아당으로부터 트레할로스 생산 최적화)

  • 조연정;고석훈;이대실;신현재
    • KSBB Journal
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    • v.18 no.1
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    • pp.8-13
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    • 2003
  • Recombinant trehalose synthase from Thermus caldophilus GK24 showed an ability to produce trehalose from maltose. The activity of the partially purified enzyme was not influenced by most metal ions at 1 mM but was inhibited by 10 mM $Co^{2+}$, $Mn^{2+}$, and $Fe^{2+}$. Enzyme activity varied during prolonged reaction due to changes in the environmental conditions. Thus, the reaction was carried out for an extended time with optimized conditions of $45^{\circ}C$ and pH 7.0. An yield of 32.9% was reached at $60^{\circ}C$ after reaction for 22 h, and, maximum trehalose conversion (69.2%) was attained at $25^{\circ}C$. The yields obtained using enzyme dosages of 10, 25, and 50 U/g were 62.3, 62.3 and 59.0 %, respectively, though the initial conversion rate was higher when the higher dose was used. Similar profiles of trehalose production yields were observed with reaction working volumes of 10 ml to 2,000 ml.

The Effects of Composition and Microstructure Variation on the Oxidation Characteristics of Stainless Steels Manufactured by Powder Metallurgy Method (분말야금 스테인리스 스틸의 산화특성에 미치는 조성 및 조직변화의 영향)

  • Lee, Jong-Pil;Hong, Ji-Hyun;Park, Dong-Kyu;Ahn, In-Shup
    • Journal of Powder Materials
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    • v.22 no.1
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    • pp.52-59
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    • 2015
  • As well-known wrought stainless steel, sintered stainless steel (STS) has excellent high-temperature anti-corrosion even at high temperature of $800^{\circ}C$, and exhibits good corrosion resistance in air. However, when temperature increases above $900^{\circ}C$, the corrosion resistance of STS begins to deteriorate and dramatically decreases. In this study, the effects of phase and composition of STS on high-temperature corrosion resistances are investigated for STS 316L, STS 304 and STS 434L above $800^{\circ}C$. The morphology of the oxide layers are observed. The oxides phase and composition are identified using X-ray diffractometer and energy dispersive spectroscopy. The results demonstrate that the best corrosion resistance of STS could be improved to that of 434L. The poor corrosion resistance of the austenitic stainless steels is due to the fact that $NiFe_2O_4$ oxides forming poor adhesion between the matrix and oxide film increase the oxidation susceptibility of the material at high temperature.

Comparison of Nutrient Intakes and Serum Mineral Levels between Smokers and Non-Smokers (흡연자와 비흡연자의 영양 섭취상태와 혈중 무기질 함량 비교)

  • 김순경;연보영;최미경
    • Journal of Nutrition and Health
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    • v.36 no.6
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    • pp.635-645
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    • 2003
  • This study was conducted to investigate the effects of smoking on nutrient intake and blood mineral status. The subjects were composed of two groups.55 smokers and 52 non-smokers. A 24-hour recall method was used along with questionnaires and serum mineral levels were analyzed by ICP spectrometer. The average ages of the smokers and non-smokers were 55.5 and 59.3 years old, respectively. The height, obesity degree, BMI, and WHR of the smokers were significantly higher than those of the non-smokers (p < 0.05, p < 0.05, p < 0.05, p < 0.01) Approximately 45.5% of the smokers smoked 16-20 cigarettes per day. The average age that the smokers started smoking was 22.0 years old and their smoking history was 33.5 years. About 74.5% of the smokers drank alcoholic beverages, while 44.2% of the non-smokers did. The smokers tended to eat less meals and breakfast meal, but drink coffee more often compared to the non-smokers. The mean daily energy intake and CPF energy intake ratio were 1231.8 ㎉ and 69.8 : 14.8 : 14.7 in the smokers and 1210.2 ㎉ and 72.1 : 14.7 12.7 in the non-smokers, respectively. The results show that the smokers tended to consume more energy, lipid, and cholesterol compared to the non-smokers. The results also show that in both groups, nutrient intake was lower than the RDA. The two groups were not significantly different in terms of the intake frequency of green-yellow vegetables and fresh fruits. There were no significant differences in serum levels of Ca, P, Mg, Cu, Fe, Mn, and Zn. However, serum Se level of the smokers was significantly higher than that of the non-smokers. In conclusion, the subjects of this study showed a serious imbalance in the nutrient intake, and the smokers showed a more undesirable dietary intake in the light of their high intake of energy, lipid, cholesterol, alcoholic beverages, and coffee. The serum Se level of the smokers was higher than that of the non-smokers, showing that Se is involved in smoking, Therefore, it could be suggested that more systematic research be conducted with respect to Se and smoking and that increased nutrition education and guidelines for smokers are required.

A Continuous Process of Persulfate Oxidation and Citric acid Washing for the Treatment of Complex-Contaminated Soil Containing Total Recoverable Petroleum Hydrocarbons and Heavy Metals (TRPHs - 중금속 복합오염토양의 동시 처리를 위한 과황산 산화 - 구연산 세척 혼성공정 개발)

  • Yoon, Na Kyeong;Choi, Jiyeon;Shin, Won Sik
    • Journal of Environmental Science International
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    • v.27 no.1
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    • pp.1-10
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    • 2018
  • A continuous process of persulfate oxidation and citric acid washing was investigated for ex-situ remediation of complex contaminated soil containing total recoverable petroleum hydrocarbons (TRPHs) and heavy metals (Cu, Pb, and Zn). The batch experiment results showed that TRPHs could be degraded by $Fe^{2+}$ activated persulfate oxidation and that heavy metals could be removed by washing with citric acid. For efficient remediation of the complex contaminated soil, two-stage and three-stage processes were evaluated. Removal efficiency of the two-stage process (persulfate oxidation - citric acid washing) was 83% for TRPHs and 49%, 53%, 24% for Cu, Zn, and Pb, respectively. To improve the removal efficiency, a three-stage process was also tested; case A) water washing - persulfate oxidation - citirc acid washing and case B) persulfate oxidation - citric acid washing (1) - citric acid washing (2). In case A, 63% of TRPHs, 73% of Cu, 60% of Zn, and 55% of Pb were removed, while the removal efficiencies of TRPHs, Cu, Pb, and Zn were 24%, 68%, 62%, and 59% in case B, respectively. The results indicated that case A was better than case B. The three-stage process was more effective than the two-stage process for the remediation of complex-contaminated soil in therms of overall removal efficiency.

A Study on the Nutrient Intakes and Factors Related to Dietary Behavior of Women by Age Groups in Incheon (인천지역 여성들의 연령별 영양섭취실태 및 식행동 관련 요인 비교 분석)

  • Jung In Kyung
    • Korean Journal of Community Nutrition
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    • v.10 no.1
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    • pp.46-58
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    • 2005
  • The purpose of this study was to compare the nutrition intakes and factors related to dietary behaviors according to age in female. The subjects included 579 females aged 15 - 59 years. This survey was conducted using a selfadministered questionnaire to obtain data about eating behaviors, living habits, eating disorders by EAT-26 (Eating Attitude Test-26), and nutrition knowledge. In younger women aged 15 - 20 years, the living habits related to health such as smoking, drinking and exercising were undesirable. In addition, the younger women group had significantly higher levels of skipping meals and frequency of eating snacks compared to the older women group and their eating times were not regular. And they showed a lower score of health eating index by mini dietary assessment (MDA). Although, all age groups consumed energy, Ca, Fe, and thiamin below the Korean RDA; especially, in adolescent, Ca intakes ($67.1\%$ RDA) were extremely low. In addition, mean score of EAT-26 was significantly higher in young women aged 15 - 29 years than older women. Also, they had rather lower levels of accuracy and perception for nutrition knowledge compared to older age group. These results suggested that Korean adolescent had undesirable nutritional intakes and attitude, and nutrition knowledge, indicating inadequate eating behaviors. These poor dietary behaviors can affect the health status. Therefore, the nutrition counseling and education to help people to have correct nutrition knowledge and to form better eating habits needs to be established.

Studies on Ferrokinetics and Copper Metabolism in Various Malignant Tumors (각종악성종양환자(各種惡性腫瘍患者)의 Ferrokinetics 및 동대사(銅代謝)에 관(關)한 연구(硏究))

  • Kim, Yong-Kyu
    • The Korean Journal of Nuclear Medicine
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    • v.1 no.1
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    • pp.21-35
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    • 1967
  • Anemia is a usual finding in advanced malignant diseases. Various mechanisms were reported as to be involved in the development of anemia of this kind, and they may differ in individual cases. Tumor anemias may be due, for instance, to chronic blood loss, shortened life span of the red blood cells or a decreased hemopoiesis in the bone marrow. The serum iron and copper levels, total iron binding capacity, apparent half survival of $^{51}Cr$-labelled red blood cells were measured along with the ferrokinetic studies using $^{59}Fe$ in 64 patients with various malignant tumors. Following were the results: 1. The serum iron levels were decreased in all cases. There existed no correlation between the serum iron levels and the severity of the diseases. 2. The serum copper levels were increased, particularly in lung cancer, rectal cancer, hepatoma and various sarcomas. There was also no correlation between the serum copper levels and the severity of the diseases. 3. The serum iron levels appeared to be inversely proportional to the serum copper levels. 4. The total iron binding capacities were within normal limits in all cases. There were also no correlations between the total iron binding capacities, serum iron levels and the severity of the diseases. 5. The patients could be classified according to the ferrokinetic patterns, namely, that of iron deficiency anemia in 10 cases, that of refractory anemia in 6 cases, normal in 1 case and that of atypical abnormal in 9 cases. 6. Apparent half survival time of $^{51}Cr$-labelled red blood cells were definitely shortened in half of the cases.

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Rutin, Catechin Derivatives, and Chemical Components of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Sprouts

  • Lee, Hee-Sun;Park, Cheol-Ho;Park, Byoung-Jae;Kwon, Soon-Mi;Chang, Kwang-Jin;Kim, Sun-Lim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.277-282
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    • 2006
  • The aim of this study was to develop the tartary buckwheat (Fagopyrum tataricum Gaertn.) sprouts and to clarify the biological and chemical characteristics of the sprouts. At 7 days after seeding, hypocotyls length and thickness, and root length of tartary buckwheat sprouts were 137 cm, 1.4 mm, and 12.6 cm, respectively. Fresh weight, dry weight, and moisture contents of an individual sprout at 7 days after seeding were 202 mg, 5.4 mg, and 95.3%, respectively. Protein content in tartary buckwheat sprouts was 23.0% which relatively higher than that of seeds, while lipid and ash contents were 3.5% and 5.3%. Among 7 minerals, the content of phosphorus showed the highest level (1,383.5 mg/100 g), while the contents of sodium and potassium were 1,197.5 mg/100 g and 1,106 mg/100 g, respectively. The contents of other minerals were Mg (795.5 mg/100 g), Ca (149 mg/100 g), Zn (16.4 mg/ 100 g), and Fe (14.7 mg/100 g). The rutin content of tatary buckwheat sprouts including root parts was the highest (5644.9 mg/100 g) at 7 days after seeding. The concentration of catechin derivatives in tartary buckwheat sprouts was high in order of catechin (59 mg/100 g), epicatechin gallate (47 mg/100 g), and epicatechin (14 mg/100 g).