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Effects of proteinate complex zinc on growth performance, hepatic and splenic trace elements concentrations, antioxidative function and immune functions in weaned piglets

  • She, Yue;Huang, Qiang;Li, Defa;Piao, Xiangshu
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.8
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    • pp.1160-1167
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    • 2017
  • Objective: To assess the effects of proteinate complex zinc (PC-Zn) on growth performance, antioxidative function, trace element concentrations and immune function in weaned piglets. Methods: Three hundred newly weaned barrows ($Duroc{\times}Landrace{\times}Yorkshire$), 28 days of age, were randomly allotted to 3 dietary groups of 5 replicate pens per group for 4 weeks of feeding. Experimental diets were: i) zinc deficient diet (ZnD, 24 mg/kg Zn supplementation from $ZnSO_4$), ii) inorganic Zn diet supplemented with 120 mg/kg of Zn from Zn sulfate ($ZnSO_4$), and iii) organic Zn diet supplemented with 120 mg/kg of Zn from PC-Zn. The body weight of pigs were recorded at the beginning, at the middle and at the end of the experiment, and the amount of feed supplied each day was recorded. Five barrows from each dietary treatment group were selected to be anesthetized and euthanized at the end of the trial to determine the Zn, Cu, Fe, and Mn concentrations, the hepatic metallothionein content, the levels of methane dicarboxylic aldehyde (MDA), Mn, and Cu/Zn superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the spleen, the levels of interleukin (IL)-2, IL-4, IL-10, interferon $(IFN)-{\gamma}$, $CD3^+$, $CD4^+$, and $CD8^+$ T lymphocyte. Results: The accumulation of Zn in the spleen, levels of SOD, GSH-Px, IL-4, IL-10, the proportions of $CD3^+$ and $CD4^+$ T lymphocyte, and the ratio of $CD4^+/CD8^+$ T lymphocyte were increased by organic Zn supplementation compared to ZnD, while the levels of MDA, $IFN-{\gamma}$, and proportion of $CD8^+$ T lymphocyte were lowered. Conclusion: These findings indicate that Zn can improve the antioxidant potential and immune functions of weaned piglets.

Corium melt researches at VESTA test facility

  • Kim, Hwan Yeol;An, Sang Mo;Jung, Jaehoon;Ha, Kwang Soon;Song, Jin Ho
    • Nuclear Engineering and Technology
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    • v.49 no.7
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    • pp.1547-1554
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    • 2017
  • VESTA (Verification of Ex-vessel corium STAbilization) and VESTA-S (-small) test facilities were constructed at the Korea Atomic Energy Research Institute in 2010 to perform various corium melt experiments. Since then, several tests have been performed for the verification of an ex-vessel core catcher design for the EU-APR1400. Ablation tests of an impinging $ZrO_2$ melt jet on a sacrificial material were performed to investigate the ablation characteristics. $ZrO_2$ melt in an amount of 65-70 kg was discharged onto a sacrificial material through a well-designed nozzle, after which the ablation depths were measured. Interaction tests between the metallic melt and sacrificial material were performed to investigate the interaction kinetics of the sacrificial material. Two types of melt were used: one is a metallic corium melt with Fe 46%, U 31%, Zr 16%, and Cr 7% (maximum possible content of U and Zr for C-40), and the other is a stainless steel (SUS304) melt. Metallic melt in an amount of 1.5-2.0 kg was delivered onto the sacrificial material, and the ablation depths were measured. Penetration tube failure tests were performed for an APR1400 equipped with 61 in-core instrumentation penetration nozzles and extended tubes at the reactor lower vessel. $ZrO_2$ melt was generated in a melting crucible and delivered down into an interaction crucible where the test specimen is installed. To evaluate the tube ejection mechanism, temperature distributions of the reactor bottom head and in-core instrumentation penetration were measured by a series of thermocouples embedded along the specimen. In addition, lower vessel failure tests for the Fukushima Daiichi nuclear power plant are being performed. As a first step, the configuration of the molten core in the plant was investigated by a melting and solidification experiment. Approximately 5 kg of a mixture, whose composition in terms of weight is $UO_2$ 60%, Zr 10%, $ZrO_2$ 15%, SUS304 14%, and $B_4C$ 1%, was melted in a cold crucible using an induction heating technique.

Comparison of Biological Activities of Extracts from Different Parts and Solvent Fractions in Cornus kousa Buerg (산딸나무의 부위별 추출물 및 용매 분획물의 생리활성 비교)

  • Kim, Young-Jung;Jeong, Jin-A;Kwon, Su-Hyun;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.28-35
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    • 2008
  • Several extracts of different parts and solvent fractions of Cornus kousa were obtained and their functional material contents, antioxidant activities and tyrosinase inhibition effects were determined. Content of total polyphenols and flavonoids contents in flower were 169.638 $mg{\cdot}g^{-1}$ and 25.418 $mg{\cdot}g^{-1}$, respectively, which were much higher than those of other parts. Also, flower extracts showed the strongest effects on DPPH and ABTS radicals scavenging and ferrous ion chelating. In flower, leaf, and stem extracts, inhibition effects on peroxidation of linoleic acid determined by ferric thiocyanate(FTC) method were higher than a synthetic antioxidant, BHT. Tyrosinase inhibition activities were shown only in flower extract. Flower and leaf extracts, showing high biological activities in various system, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. Total polyphenol contents of water fractions were higher than any other solvent fractions in both flower and leaf, 67.006 $mg{\cdot}g^{-1}$ and 67.739 $mg{\cdot}g^{-1}$, respectively. But total flavonoid contents were higher in ethyl acetate fraction for flower extract and butanol fraction for leaf extract. Among the solvent fractions, the highest efficiency of free radical scavenging activities was obtained in ethyl acetate fraction for flower extract and n-butanol fraction for leaf extract. Tyrosinase inhibition activities were higher in water fraction for both flower and leaf extracts, 49.24% and 31.8%, respectively.

Stability of Anthocyanin Pigment from Juice of Raspberries (나무딸기(복분자(覆盆子)) 과즙색소(果汁色素) Anthocyanin의 안정성(安定性)에 관한 연구(硏究))

  • Park, Jung-Mi;Joo, Kwang-Jee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.3
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    • pp.67-74
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    • 1982
  • The effect of various pH levels, temperatures. organic acids, inorganic salts, metal ions on the stability of the anthocyanins pigment (pH 3.7) from the juice of raspberries were investigated. Initial absorption of total anthocyanin was decreased as pH increased from 1.0 to 7.0. Total amount of anthocyanin reached the highest at pH 3.7 and least at pH 7.0. The total anthocyanin content decreased rapidly with the increasing temperature. Many organic acids were found to enrich and stabilize the color density at 520nm in anthocyanin solution (pH 3.7). The hyperchromic effect of saturated n-carboxylic acid increased in the following order; formic acid> acetic acid>n-butyric acid>propionic acid. On the polycarboxylic acid, especially, malic acid showed 550$\sim$930% higher than control group. On the inorganic salts (0.5M), sodium perchlorate had the most hyperchromic effect and followed by sodium sulfate>sodium chloride>sodium phosphate, monobasic. Among the metal ions, both aluminium ion and cupric ion much more accelerated the anthocyanins degradation as compared with other metal ions.

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A Study on the Antioxidant Activity of Hae-Songi Mushroom(Hypsizigus marmoreus) Hot Water Extracts (해송이 버섯 열수 추출물의 항산화 효과에 관한 연구)

  • Xu, Xiao-Mei;Jun, Joon-Young;Jeong, In-Hak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.11
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    • pp.1351-1357
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    • 2007
  • "Hae-Songi" mushroom is a kind of Hypsizigus marmoreus, one of the edible mushrooms. Powder and hot water extracts of the mushroom fruit-body were investigated for their proximate composition, amino acid contents, ${\beta}-glucan$ contents, total phenolic contents and antioxidant activity. The measured antioxidant activity included free radical scavenging activity against DPPH, reducing power $Fe^{2+}$ chelating ability and SOD activity. Mushroom extracts exhibited in vitro antioxidant activity. This mushroom contained high protein (29%, total amino acid contents 204.86 mg/g), free amino acids (46.53 mg/g) and ${\beta}-glucan$(0.11%). At a concentration of 1% extracts solutions (w/v) according to different extraction times, DPPH free radical-scavenging activities were found to exhibit $89%{\sim}92%$ inhibition. Positive correlations $(R^2=0.9901{\sim}0.7424)$ were found between total phenolic content in the mushroom hot water extracts and their antioxidant activity. In this study, it is demonstrated that "Hae-Songi" mushroom may possess potential for use as a health food, due to theirantioxidant capacity.

Factors Affecting Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent (다양한 페놀성 물질과 Folin-Ciocalteu 시약의 반응성에 미치는 영향 요인 평가)

  • Hong, Jung-Il;Kim, Hyun-Jung;Kim, Ji-Yun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.205-213
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    • 2011
  • The Folin-Ciocalteu (F-C) reagent has been extensively used for quantifying total phenolic contents in many different types of food materials. Since several different procedures of the assay methods using the F-C reagent have been applied, we investigated changes in reactivity of various phenolic compounds with the F-C reagent under three different assay conditions and factors affecting reactivity. Among 10 standard compounds tested, compounds with high hydroxyl density (number of -OH/molecular weight) showed a largely different response according to addition sequence of the F-C reagent or $Na_2CO_3$. Preincubation in $Na_2CO_3$ significantly reduced the reactivity of the phenolic compounds bearing galloyl moiety (e.g. gallic acid, tannic acid, and epigallocatechin-3-gallate) with the F-C reagent, while monophenol compounds including ferulic acid and sinapinic acid were more stable as compared to diphenols. There was little change in response to the F-C reagent of all phenolic compounds incubated in acidic pH; their reactivity except ferulic acid was reduced significantly when incubated in neutral or alkaline pH. Changes in reactivity of gallic acid incubated in $Na_2CO_3$ or neutral/alkaline pH conditions were the most prominent. $H_2O_2$ generated from phenolic compounds did not affect the reaction with the F-C reagents. The present results suggest that reactivity of different phenolic compounds with F-C reagent was affected considerably by different procedures of the assay, and the total phenolic contents could be fluctuated according to standard compounds and assay scheme.

Screening of the Biologoical Activity from Water Extracts of the Medicinal Plants and the Protective Effect of R. palmatum on MTPT-induced Neurotoxicity (한약재 물 추출물의 생리활성 검색 및 MPTP-유도 신경독성에 대한 대황의 보호효과)

  • Kim Tae Eun;Yoon Yeo Min;Park Yong In;Kim Youn Seok;Jeon Byung Hun;Kim Myung Dong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.6
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    • pp.1666-1685
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    • 2004
  • This present study was designed to screen medicinal plants for the treatment of brain diseases such as Parkinson's disease or aging. We tested the effects of the water extracts from 38 species medicinal plants on antioxidant capacity, monoamine oxidase B (MAO-B) inhibitory activity, acetylcholinesterase (AChE) inhibition and antiperoxidation activity in vitro. The water extracts from 38 species were tested on their antioxidant activity using radical scavenging effects against ABTS+. The water extract of C. sappan was showed the highest antioxidant capacity, the antioxidant activity at 1 Jig of herbal extract being 0.38mM TE. Lipid peroxidation in brain homogenates induced by NADPH and ADP-Fe/sup 2+/ was strong inhibited by C. sappan and R. palmatum extracts. Among the 38 medicinal plants investigated, R. palmatum showed significant biological activity (antioxidant capacity, MAO-B inhibiory activity, and AChE inhibitory activity). The protective efficacy of R. palmatum water extract on 1-methyl-4­phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism and its possible mechanism were studied in C57BL/6 mice. Treatment of R. palmatum water extract protected biomacromolecules such as lipids from oxidative damage induced by MPTP. The content of MDA in brain tissue was decreased significantly by R. palmatum extract. These results suggest that R. palmatum water extract plays on effective role in attenuating MPTP-induced neurotoxicity in mice. This protective effect of R. palmatum might be estimated the result from the inhibitory activity on monoamine oxidase B and the enhancement of antioxidant activity.

Properties of Foamed Concrete According to Types and Concentrations of Foam Agent (기포제 종류 및 희석 농도에 따른 기포 콘크리트의 특성)

  • Kim, Jin-Man;Jeong, Ji-Yong;Hwang, Eui-Hwan;Shin, Sang-Chul
    • Journal of the Korea Concrete Institute
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    • v.24 no.1
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    • pp.61-70
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    • 2012
  • Recently, the government has been working feverously to save energy and reduce greenhouse gas emission by enacting Basic Act on Low Carbon Green Growth at the national level. Improving the insulation performance of building exterior and insulator can reduce the energy in the building sector. This study is about developing light-weight foamed concrete insulation panel that can be applied to buildings to save energy and to find the optimal condition for the development of insulation materials that can save energy by enhancing its physical, kinetic and thermal characteristics. Various experimental factors and conditions were considered in the study such as foam agent types (AES=Alcohol Ethoxy Sulfate, AOS=Alpha-Olefin Sulfonate, VS=Vegetable Soap, FP=Fe-Protein), foam agent dilution concentration (1, 3, 5%), and foam percentage (30, 50, 70%). Experiment results indicated that the surface tension of aqueous solution including foam agent, was lower when AOS was used over other foam agents. FP produced relatively stable foams in 3% or more, which produced unstable foams containing high water content and low surface tension when diluted at low concentration. Depending on foam agent types, compressive strength and thermal conductivity were similar at low density range but showed some differences at high concentration range. In addition, when concentrations of foam agent and foaming ratio increased, pore size increased and open pores are formed. In all types of foam agent, thermal conductivity were excellent, satisfying KS standards. The most outstanding performance for insulation panel was obtained when FP 3% was used.

Manufacturing Characteristics of Black Burnished Pottery from Pungnaptoseong, Beakje (백제 풍납토성 출토 흑색마연토기의 제작 특성)

  • Kim, Su Kyoung;Han, Min Su;Nam, Sang Won;Jang, Sungyoon
    • Journal of Conservation Science
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    • v.33 no.6
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    • pp.417-429
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    • 2017
  • This study aimed at the identification of the black coating materials on the pottery surface and manufacturing technique of black burnished pottery excavated from the Pungnaptoseong, Seoul, which is estimated to be royal fortress of Beakje. According to observation of black coated surface and raw materials, potteries can be divided into two groups. The first group potteries have black inner and black surface with well-selected particles. Second group potteries are black in surface only with unevenly selected particles. Each group seems to represent different manufacturing technique in clay selection, color development timing and method. The black burnished pottery contains high values of CaO, $P_2O_5$, L.O.I. and lower content of $Fe_2O_3$ compared with gray pottery excavated from the same site, which indicates plant ashes were used for coloring the surface of pottery in black. According to the result of SEM-EDS mapping of black burnished pottery, carbon was concentrated on pottery surface, while iron was concentrated on the surface of the gray pottery. Based on XRD analysis, firing temperature of the black burnished potteries were fired low temperature range at 700 to $900^{\circ}C$, and that of the gray potteries ranged from $900^{\circ}C$ to $1000^{\circ}C$.

Chemical Characteristics and Enzyme Activities of Icheon Ge-Geol Radish, Gangwha Turnip, and Korean Radish (이천 게걸무, 강화 순무, 조선무의 화학적 특성 및 효소활성)

  • Kim, Haeng-Ran;Lee, Ji-Hyun;Kim, Yang-Suk;Kim, Kyung-Mi
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.255-259
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    • 2007
  • For this investigation, we analyzed the chemical characteristics and enzyme activities of Icheon Ge-Geol radish, Gangwha turnip, and Korean radish. Regarding their proximate compositions, the water contents of the Icheon Ge-Geol radish, Gangwha turnip, and Korean radish were 87.78, 92.73, and 91.45%, respectively. The crude protein, crude fiber, and ash contents of the Icheon Ge-Geol radish were 1.35, 1.11, and 1.55%, respectively, which were much higher than the contents of the other samples; however, total dietary fiber was significantly lower. For mineral content, the magnesium, potassium, and calcium levels of the Icheon Ge-Geol radish were higher than the other samples. With regards to the enzyme activities of the samples, the protease and myrosinase activities of the Icheon Ge-Geol radish were higher than in the other samples. For ${\alpha}-amylase$ activity, the Korean radish showed the strongest activity (18.99 units/g of sample), followed by the Gangwha turnip and Icheon Ge-Geol radish.