• 한국가정과교육학회지
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• 제28권2호
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• pp.61-78
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• 2016

본 연구의 목적은 남북한 가정생활분야 관련용어 가운데 아동 가족분야의 용어를 비교 고찰함으로써 통일 이후 가정생활교육의 효율성을 도모하기 위한 기초자료를 제공하는 것이다. 남북한 용어비교를 위한 자료 수집은 북한 용어 사전, 통일부 및 정부기관 발행보고서와 관련 웹사이트, 북한의 가정생활을 주제로 한 단행본을 활용하였고, 수집된 용어는 '2015 개정 교육과정'의 고등학교 기술 가정 교과 내용체계 가운데 '인간발달과 가족'영역을 기준으로 분석하였다. 연구결과, 첫째, 발달영역용어가 관계영역용어보다 큰 차이를 보였으며, 발달영역 중에서는 '사랑과 결혼' '임신 중 생활과 출산'에서, 관계영역 중에서는 '자녀 돌보기'에서 큰 차이를 보였다. 둘째, 남북한 용어에서 차이를 보이는 주요 내용은 호칭관련, 혼례관련, 임신관련, 출산관련, 자녀돌봄관련 내용이었다. 셋째, 남북한 용어 차이의 주요 양상은 상이한 남북한 사회체제로 인한 차이, 말다듬기로 인한 차이, 표기법으로 인한 차이가 주를 이루었다. 본 연구는 남북한 아동 가족분야 용어 비교를 통해 통일 이후 가정생활교육의 기초자료를 제시하였다는 점에서 의의를 찾을 수 있다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제19권1호
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• pp.3-12
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• 2008

Objectives : Child sexual abuse is a highly prevalent societal problem that cuts across all ethnic, racial, educational, and socioeconomic groups. There has been mounting empirical evidence for the efficacy of manual-based, cognitive behavioral treatment(CBT) for sexually abused children. Therefore, the aim of this review was to address the rationale and efficacy of CBT for sexually abused children, and to introduce a broad outline of a typical CBT program for young people suffering the emotional and behavioral consequences of sexual abuse. Methods : A selective literature review was undertaken. Results : To date, trauma-focused CBT is the most effective treatment for the sexually abused child. The rationale and efficacy of CBT was reviewed and a typical CBT program was outlined session by session. Conclusion : Based on trauma-focused CBT for sexually abused children, the results demonstrate the need for future development of a standard CBT program for the Korean population.

• 가정과삶의질연구
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• 제30권3호
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• pp.83-103
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• 2012

This thesis is based on an in-depth interview on participation experience of ten immigrants who reside in S Multi-cultural Family Support Center that is located in Gyeonggi-do in Korea Immigration & Integration Program(KIIP). The purpose of this research is to present through it's basic data for improvement in the operation of KIIP in the future by grasping participation process in KIIP that the immigrants have experienced, problems involved in their operation, and related requirements. Major results of the research are as follows: First, the motive for the immigrants' participation in KIIP was to acquire Korean nationality, learn Korean, and prepare for their future in Korea. Second, as a difficulty in participation in KIIP, access to educational institutions loomed large. Third, regarding improvements in the operation of KIIP, marriage immigrants needed to continue Korean language education, whereas other immigrants revealed a demand for opening evening classes or weekend classes. In the final analysis, it seems that for KIIP to provide an opportunity for the immigrants to have a vision for their life in the future, as well as for its realization in Korean society, policy-oriented institutional support that pays attention to their life situation and demands is badly needed.

• 한국조경학회지
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• 제24권4호
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• pp.85-95
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• 1997

The purpose of this study is to investigate the function of "Chang-Won-Seo" which is one of government organizations in charge of landscape architecture during Chosun-Dynasty This study is based on the analysis of historic documents published by the government offices during Chosen-dynasty. The landscape architectural administration during Chosun-Dynasty was mainly undertaken by the Ministry of Industry in both central and local government. Especially, the government office titled "Seon-Gong-Gam" under the Ministry of Industry was in charge of landscape construction. There were seven divisions under the "Chang-Won-Seo" and three divisions such as "Kwa-Won-Saek" , "Saeng-Gwa-Saek" and "Keon-Gwa-Saek" took charge of main part of the work. "Kwa-Won-Saek" was In charge of the management of public fruit gardens and lotus ponds constructed in Han-Yang(Seoul) in order to harvest fruit and edible lotus seeds. "Saeng-Gwa-Saek" was responsible for the supply of fresh fruits to the royal family and the government offices while "Keon-Gwa-Saek" took charge of supplying them with dried fruits. In view of the responsibilities of three divisions, it is concluded that the function of "Chang-Won-Seo" was not to construct and manage the pleasure gardens controlled by the government. but to manage the public fruit gardens and to supply the royal family and the government offices with fresh and dried fruits.

• Journal of Animal Science and Technology
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• 제57권12호
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• pp.44.1-44.9
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• 2015

Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.888-897
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• 2009

Lipase diversity in glacier soil was assessed by culture-independent metagenomic DNA fragment screening and confirmed by cell culture experiments. A set of degenerate PCR primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly PCR amplify metagenomic DNA from glacier soil. These products were used to construct a lipase fragment clone library. Among the 300 clones sequenced for the analysis, 201 clones encoding partiallipases shared 51-82% identity to known lipases in GenBank. Based on a phylogenetic analysis, five divergent clusters were established, one of which may represent a previously unidentified lipase subfamily. In the culture study, 11 lipase-producing bacteria were selectively isolated and characterized by 16S rDNA sequences. Using the above-mentioned degenerate primers, seven lipase gene fragments were cloned, but not all of them could be accounted for by the clones in the library. Two full-length lipase genes obtained by TAIL-PCR were expressed in Pichia pastoris and characterized. Both were authentic lipases with optimum temperatures of ${\le}40^{\circ}C$. Our study indicates the abundant lipase diversity in glacier soil as well as the feasibility of sequence-based screening in discovering new lipase genes from complex environmental samples.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.520-528
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• 2021

Plesiomonas shigelloides, a member of the family Vibrionaceae, is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. P. shigelloides has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. P. shigelloides contains 102 O-antigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new P. shigelloides serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between rep and aqpZ. Only O38 possesses the wzm and wzt genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using wzx and wzy showed that both genes are diversified. Among the nine new P. shigelloides serogroups, eight use wzx/wzy genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.99-109
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• 2022

This study is the first report on production and characterization of the enzyme from an Ornithinibacillus species. A 4.2-fold increase in the extracellular protease (called L9^T) production from Ornithinibacillus caprae L9^T was achieved through the one-factor-at-a-time approach and response surface methodological optimization. L9^T protease exhibited a unique protein band with a mass of 25.9 kDa upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This novel protease was active over a range of pH (4-13), temperatures (30-80℃) and salt concentrations (0-220 g/l), with the maximal activity observed at pH 7, 70℃ and 20 g/l NaCl. Proteolytic activity was upgraded in the presence of Ag⁺, Ca²⁺ and Sr²⁺, but was totally suppressed by 5 mM phenylmethylsulfonyl fluoride, which suggests that this enzyme belongs to the serine protease family. L9^T protease was resistant to certain common organic solvents and surfactants; particularly, 5 mM Tween 20 and Tween 80 improved the activity by 63 and 15%, respectively. More importantly, L9^T protease was found to be effective in dehairing of goatskins, cowhides and rabbit-skins without damaging the collagen fibers. These properties confirm the feasibility of L9^T protease in industrial applications, especially in leather processing.

• Journal of Veterinary Science
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• 제21권4호
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• pp.63.1-63.9
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• 2020

Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

• Natural Product Sciences
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• 제28권4호
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• pp.168-180
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• 2022

Ephedra is a genus of the Ephedraceae family and is found in temperate regions, such as Central Asia and Europe. Among the various ephedra species, Ma Huang (Ephedra herb) is derived from the aerial parts of Ephedra sinica S tapf, Ephedra equisetina Bunge, and Ephedra intermedia Schrenk & C.A. Mey. Ma Huang contains various ephedra alkaloids, including (-)-ephedrine, (+)-pseudoephedrine, (-)-norephedrine, (+)-norpseudoephedrine, (-)-methylephedrine, and (+)-methylpseudoephedrine, which are found naturally as single enantiomers, although they can be prepared as racemates. Although the use of Ma Huang in foods is prohibited in Korea, products containing Ma Huang can be imported, and so it is necessary to develop a suitable analytical technique for the detection of Ma Huang in foods. Herein, we report the development of analytical methods for the detection of ephedra alkaloids in products containing Ma Huang. Following sample purification by solid phase extraction, quantitative analysis was performed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). Additionally, the enantiomers were successfully separated using HPLC-DAD. We successfully analyzed various food samples, where the ephedra alkaloids were qualitatively and quantitatively determined, and the enantiomers were separated. It is expected that these methods may contribute toward preventing the distribution of illegal products containing Ma Huang.