Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Analysis and Serological Detection of Novel O-Antigen Gene Clusters of Plesiomonas shigelloides

  • Wang, Xiaochen (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University) ;
  • Xi, Daoyi (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University) ;
  • Li, Yuehua (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University) ;
  • Yan, Junxiang (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University) ;
  • Zhang, Jingyun (State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention) ;
  • Guo, Xi (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University) ;
  • Cao, Boyang (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University)
  • Received : 2020.10.05
  • Accepted : 2021.02.04
  • Published : 2021.04.28
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Abstract

Plesiomonas shigelloides, a member of the family Vibrionaceae, is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. P. shigelloides has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. P. shigelloides contains 102 O-antigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new P. shigelloides serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between rep and aqpZ. Only O38 possesses the wzm and wzt genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using wzx and wzy showed that both genes are diversified. Among the nine new P. shigelloides serogroups, eight use wzx/wzy genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.

Keywords

References

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