Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

  • Yang, Dong-Kun (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA)) ;
  • Kim, Ha-Hyun (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA)) ;
  • Lee, Siu (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA)) ;
  • Oh, Dongryul (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA)) ;
  • Yoo, Jae Young (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA)) ;
  • Hyun, Bang-Hun (Viral Disease Research Division, Animal and Plant Quarantine Agency (APQA), Ministry for Agriculture, Food and Rural Affairs (MAFRA))
  • Received : 2019.10.25
  • Accepted : 2020.04.16
  • Published : 2020.07.31
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Abstract

Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

Keywords

Acknowledgement

This study was financially supported by a grant (B-1543083-2018-19-04) from the Animal, and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.

References

  1. Appel M, Carmichael LE, Robson DS. Canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody. Am J Vet Res. 1975;36(08):1199-1202.
  2. Benetka V, Weissenbock H, Kudielka I, Pallan C, Rothmuller G, Mostl K. Canine adenovirus type 2 infection in four puppies with neurological signs. Vet Rec. 2006;158(3):91-94. https://doi.org/10.1136/vr.158.3.91
  3. Takamura K, Ajiki M, Hiramatsu K, Takemitsu S, Nakai M, Sasaki N. Isolation and properties of adenovirus from canine respiratory tract. Nippon Juigaku Zasshi. 1982;44(2):355-357. https://doi.org/10.1292/jvms1939.44.355
  4. Timurkan MO, Aydin H, Alkan F. Detection and molecular characterization of canine adenovirus type 2 (CAV-2) in dogs with respiratory tract symptoms in shelters in Turkey. Vet Arh. 2018;88(4):467-479. https://doi.org/10.24099/vet.arhiv.0052
  5. Yang DK, Kim HH, Yoon SS, Lee H, Cho IS. Isolation and identification of canine adenovirus type 2 from a naturally infected dog in Korea. Korean J Vet Res. 2018;58(4):177-182. https://doi.org/10.14405/kjvr.2018.58.4.177
  6. Tham KM, Horner GW, Hunter R. Isolation and identification of canine adenovirus type-2 from the upper respiratory tract of a dog. N Z Vet J. 1998;46(3):102-105. https://doi.org/10.1080/00480169.1998.36068
  7. Yang DK, Kim HH, Yoon SS, Ji M, Cho IS. Incidence and sero-survey of canine adenovirus type 2 in various animal species. J Bacteriol Virol. 2018;48(3):102-108. https://doi.org/10.4167/jbv.2018.48.3.102
  8. Wang S, Wen Y, An T, Duan G, Sun M, Ge J, et al. Development of an immunochromatographic strip for rapid detection of canine adenovirus. Front Microbiol. 2019;10:2882. https://doi.org/10.3389/fmicb.2019.02882
  9. Yoon SS, Byun JW, Park YI, Kim MJ, Bae YC, Song JY. Comparison of the diagnostic methods on the canine adenovirus type 2 infection. Basic Appl Pathol. 2010;3(2):52-56. https://doi.org/10.1111/j.1755-9294.2010.01073.x
  10. Noon KF, Rogul M, Binn LN, Keefe TJ, Marchwicki RH, Thomas R, et al. An enzyme-linked immunosorbent assay for the detection of canine antibodies to canine adenoviruses. Lab Anim Sci. 1979;29(5):603-609.
  11. Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type-specific by virus neutralization and immunofluorescence. Vet Microbiol. 1988;16(1):1-8. https://doi.org/10.1016/0378-1135(88)90121-6
  12. Segura MM, Puig M, Monfar M, Chillon M. Chromatography purification of canine adenoviral vectors. Hum Gene Ther Methods. 2012;23(3):182-197. https://doi.org/10.1089/hgtb.2012.058
  13. Yang DK, Kim HH, Lee S, Li M, Han BH, Oh S, et al. Development of an indirect ELISA featuring plates coated with column chromatographically purified canine adenovirus type-1 antigen. J Bacteriol Virol. 2020;50(1):17-24. https://doi.org/10.4167/jbv.2020.50.1.017
  14. Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Epidemiol. 1938;27(3):493-497. https://doi.org/10.1093/oxfordjournals.aje.a118408
  15. Yang DK, Kim HH, Lee SH, Ji M, Cho IS. Indirect ELISA for the detection of rabies virus antibodies in dog sera. J Bacteriol Virol. 2017;47(3):148-155. https://doi.org/10.4167/jbv.2017.47.3.148
  16. Muhamuda K, Madhusudana SN, Ravi V. Development and evaluation of a competitive ELISA for estimation of rabies neutralizing antibodies after post-exposure rabies vaccination in humans. Int J Infect Dis. 2007;11(5):441-445. https://doi.org/10.1016/j.ijid.2006.09.013
  17. Gschwender HH, Brummund M, Lehmann-Grube F. Lymphocytic choriomeningitis virus. I. Concentration and purification of the infectious virus. J Virol. 1975;15(6):1317-1322. https://doi.org/10.1128/jvi.15.6.1317-1322.1975
  18. Schagen FH, Rademaker HJ, Rabelink MJ, van Ormondt H, Fallaux FJ, van der Eb AJ, et al. Ammonium sulphate precipitation of recombinant adenovirus from culture medium: an easy method to increase the total virus yield. Gene Ther. 2000;7(18):1570-1574. https://doi.org/10.1038/sj/gt/3301285
  19. Kanegae Y, Makimura M, Saito I. A simple and efficient method for purification of infectious recombinant adenovirus. Jpn J Med Sci Biol. 1994;47(3):157-166. https://doi.org/10.7883/yoken1952.47.157
  20. Burova E, Ioffe E. Chromatographic purification of recombinant adenoviral and adeno-associated viral vectors: methods and implications. Gene Ther. 2005;12 Suppl 1:S5-S17. https://doi.org/10.1038/sj.gt.3302611
  21. He XM, Voss C, Li J. Exploring the unique selectivity of hydrophobic cation exchanger Nuvia cPrime for the removal of a major process impurity: a case study with IgM. Curr Protein Pept Sci. 2019;20(1):65-74.
  22. Konz JO, Livingood RC, Bett AJ, Goerke AR, Laska ME, Sagar SL. Serotype specificity of adenovirus purification using anion-exchange chromatography. Hum Gene Ther. 2005;16(11):1346-1353. https://doi.org/10.1089/hum.2005.16.1346
  23. Waritani T, Chang J, McKinney B, Terato K. An ELISA protocol to improve the accuracy and reliability of serological antibody assays. MethodsX. 2017;4:153-165. https://doi.org/10.1016/j.mex.2017.03.002