• Korean Journal of Veterinary Research
• /
• v.34 no.1
• /
• pp.115-125
• /
• 1994

In this study, we try to establish the rapid and specific detection system for Salmonella species. The PhoE gene of Salmonella species was amplified with two specific primers, ST5 and ST8c, using PCR. The probe prepared from the amplified PhoE gene was sequenced and applied for Southern blot analysis. After PCR with ST5 and ST8c primers for PhoE gene, DNA bands of expected size(365bp) from 7 different Salmonella species were detected, but not from 12 enterobacteriaceae and 3 gram positive bacteria. PCR was highly sensitive to detect up to 10fg of purified DNA template and to identify Salmonella species with only 320 heat-lysed bacterial cells. The inhibition of PCR amplification from stool specimen was occurred with 50-fold dilution but disappeared over 100 fold dilution of samples. It was confirmed that the PhoE genes were amplified and cloned with over 97% nacleotide sequence homology of PCR products compared with that of S. typhfmurium LT2. The DNA probe derived from S. typhimurium TA 3,000 showed highly specific and sensitive reaction with PCR products of all tested Salmonella species. These results indicate that PCR was rapid and sensitive detection method for Salmonella species and DNA probe prepared from S. typhimurium TA 3,000 was specific to identify PCR products of different Salmonella species.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.10
• /
• pp.2974-2978
• /
• 2014

$Mn_3O_4$/multi-walled carbon nanotube (MWCNT) composites are prepared by chemically synthesizing $Mn_3O_4$ nanoparticles on a MWCNT film at room temperature. Structural and morphological characterization has been carried out using X-ray diffraction (XRD) and scanning and transmission electron microscopies (SEM and TEM). These reveal that polycrystalline $Mn_3O_4$ nanoparticles, with sizes of about 10-20 nm, aggregate to form larger nanoparticles (50-200 nm), and the $Mn_3O_4$ nanoparticles are attached inhomogeneously on MWCNTs. The electrochemical behavior of the composites is analyzed by cyclic voltammetry experiment. The $Mn_3O_4$/MWCNT composite exhibits a specific capacitance of $257Fg^{-1}$ at a scan rate of $5mVs^{-1}$, which is about 3.5 times higher than that of the pure $Mn_3O_4$. Cycle-life tests show that the specific capacitance of the $Mn_3O_4$/MWCNT composite is stable up to 1000 cycles with about 85% capacitance retention, which is better than the pure $Mn_3O_4$ electrode. The improved supercapacitive performance of the $Mn_3O_4$/MWCNT composite electrode can be attributed to the synergistic effects of the $Mn_3O_4$ nanoparticles and the MWCNTs, which arises not only from the combination of pseudocapacitance from $Mn_3O_4$ nanoparticles and electric double layer capacitance from the MWCNTs but also from the increased surface area, pore volume and conducting property of the MWCNT network.

• Structural Engineering and Mechanics
• /
• v.78 no.5
• /
• pp.585-597
• /
• 2021

This paper presents a comparative study of analytical method, finite element method (FEM) and Multilayer Perceptron (MLP) for analysis of a contact problem. The problem consists of a functionally graded (FG) layer resting on a half plane and pressed with distributed load from the top. Firstly, analytical solution of the problem is obtained by using theory of elasticity and integral transform techniques. The problem is reduced a system of integral equation in which the contact pressure are unknown functions. The numerical solution of the integral equation was carried out with Gauss-Jacobi integration formulation. Secondly, finite element model of the problem is constituted using ANSYS software and the two-dimensional analysis of the problem is carried out. The results show that contact areas and the contact stresses obtained from FEM provide boundary conditions of the problem as well as analytical results. Thirdly, the contact problem has been extended based on the MLP. The MLP with three-layer was used to calculate the contact distances. Material properties and loading states were created by giving examples of different values were used at the training and test stages of MLP. Program code was rewritten in C++. As a result, average deviation values such as 0.375 and 1.465 was obtained for FEM and MLP respectively. The contact areas and contact stresses obtained from FEM and MLP are very close to results obtained from analytical method. Finally, this study provides evidence that there is a good agreement between three methods and the stiffness parameters has an important effect on the contact stresses and contact areas.

• Korean Journal of Pharmacognosy
• /
• v.51 no.4
• /
• pp.291-296
• /
• 2020

Filipendula glaberrima (FG) is a plant endemic to South Korea. It is economically important as a food source and used as a medicine in treating ailments. Filipendula flowers are characterized by the presence of several polyphenolic constituents. The aim of this study is to determine the content of (+)-catechin in Filipendula glaberrima collected from different regions at different flowering stages. High-performance liquid chromatography with a gradient elution system (0.5% acetic acid in water : acetonitrile = 95 : 5 to 0 : 100 for 35 min) was used. A reverse-phase INNO column with UV detection at 278 nm was employed. The results revealed that F. glaberrima from Mt. Odae has the highest (+)-catechin content (10.600 mg/g). Furthermore, its content was the lowest in samples collected during the pre-flowering period and the highest at the early-flowering stage. This study provides a basis in establishing the optimal period and the best region for collecting F. glaberrima with maximized (+)-catechin yield.

• Steel and Composite Structures
• /
• v.43 no.5
• /
• pp.581-601
• /
• 2022

The main objective of this research work is to investigate the free vibration behavior of annular sandwich plates resting on the Kerr foundation at thermal conditions. This sandwich configuration is composed of two FGM face sheets as coating layer and a porous GPLRC (GPL reinforced composite) core. It is supposed that the GPL nanofillers and the porosity coefficient vary continuously along the core thickness direction. To model closed-cell FG porous material reinforced with GPLs, Halpin-Tsai micromechanical modeling in conjunction with Gaussian-Random field scheme is used, while the Poisson's ratio and density are computed by the rule of mixtures. Besides, the material properties of two FGM face sheets change continuously through the thickness according to the power-law distribution. To capture fundamental frequencies of the annular sandwich plate resting on the Kerr foundation in a thermal environment, the analysis procedure is with the aid of Reddy's shear-deformation plate theory based high-order shear deformation plate theory (HSDT) to derive and solve the equations of motion and boundary conditions. The governing equations together with related boundary conditions are discretized using the generalized differential quadrature (GDQ) method in the spatial domain. Numerical results are compared with those published in the literature to examine the accuracy and validity of the present approach. A parametric solution for temperature variation across the thickness of the sandwich plate is employed taking into account the thermal conductivity, the inhomogeneity parameter, and the sandwich schemes. The numerical results indicate the influence of volume fraction index, GPLs volume fraction, porosity coefficient, three independent coefficients of Kerr elastic foundation, and temperature difference on the free vibration behavior of annular sandwich plate. This study provides essential information to engineers seeking innovative ways to promote composite structures in a practical way.

• Steel and Composite Structures
• /
• v.45 no.5
• /
• pp.729-747
• /
• 2022

The critical buckling loads and buckling modes of bi-directional functionally graded porous unified higher order shear plate with elastic foundation are investigated. A mathematical model based on neutral axis rather than midplane is developed in comprehensive way for the first time in this article. The material constituents form ceramic and metal are graded through thickness and axial direction by the power function distribution. The voids and cavities inside the material are proposed by three different porosity models through the thickness of plate. The constitutive parameters and force resultants are evaluated relative to the neutral axis. Unified higher order shear plate theories are used to satisfy the zero-shear strain/stress at the top and bottom surfaces. The governing equilibrium equations of bi-directional functionally graded porous unified plate (BDFGPUP) are derived by Hamilton's principle. The equilibrium equations in the form of coupled variable coefficients partial differential equations is solved by using numerical differential integral quadrature method (DIQM). The validation of the present model is presented and compared with previous works for bucking. Deviation in buckling loads for both mid-plane and neutral plane are developed and discussed. The numerical results prove that the shear functions, distribution indices, boundary conditions, elastic foundation and porosity type have significant influence on buckling stability of BDFGPUP. The current mathematical model may be used in design and analysis of BDFGPU used in nuclear, mechanical, aerospace, and naval application.

• Advances in nano research
• /
• v.15 no.5
• /
• pp.401-410
• /
• 2023

Vibration investigation of fluid-filled three layered cylindrical shells is studied here. A cylindrical shell is immersed in a fluid which is a non-viscous one. Shell motion equations are framed first order shell theory due to Love. These equations are partial differential equations which are usually solved by approximate technique. Robust and efficient techniques are favored to get precise results. Employment of the wave propagation approach procedure gives birth to the shell frequency equation. Use of acoustic wave equation is done to incorporate the sound pressure produced in a fluid. Hankel's functions of second kind designate the fluid influence. Mathematically the integral form of the Lagrange energy functional is converted into a set of three partial differential equations. It is also exhibited that the effect of frequencies is investigated by varying the different layers with constituent material. The coupled frequencies changes with these layers according to the material formation of fluid-filled FG-CSs. Throughout the computation, it is observed that the frequency behavior for the boundary conditions follow as; clamped-clamped (C-C), simply supported-simply supported (SS-SS) frequency curves are higher than that of clamped-simply (C-S) curves. Expressions for modal displacement functions, the three unknown functions are supposed in such way that the axial, circumferential and time variables are separated by the product method. Computer software MATLAB codes are used to solve the frequency equation for extracting vibrations of fluid-filled.

• Journal of radiological science and technology
• /
• v.27 no.2
• /
• pp.35-43
• /
• 2004

Radioprotective effects of ginseng extracts on liver damage induced by high energy x-ray were studied. To one group of ICR male mice were given white(50 mg/kg/day for 7days, orally) and fermenta ginseng extracts(500 mg/kg/day for 7days, orally) before irrdiation. To another group were irradiated by 5 Gy dose of high energy x-ray. Contrast group were given with saline(0.1 ml). This study also investigated the radioprotective effect between SOD, CAT, hydrogen peroxide and ginseng extracts on hepatic damage. This study measured the level of superoxide dismutase(SOD), catalase(CAT), hydrogen peroxide($H_2O_2$) in liver tissue. Administrating orally white (50 mg/kg/day for 7days, orally) and fermenta ginseng extracts(500 mg/kg/day), the activity of SOD, CAT were generally increased and the hydrogen peroxide($H_2O_2$) was decreased. After irradiation, the activity of SOD, CAT were generally decreased and the hydrogen peroxide($H_2O_2$) was increased. Therefore, ginseng extracts increased antioxidative enzyme activity. And We know that the antioxidatant effect of extracts from white and fermenta ginseng protect radiation damage by direct antioxidant effect involving SOD, CAT. It was included that ginseng can protect against radiation damage through its antioxidatant properties.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.5
• /
• pp.745-752
• /
• 2015

Tuberculosis (TB) is the most common mycobacterial infection in developing countries, requiring a rapid, accurate, and well-differentiated detection/diagnosis. For the rapid detection and discrimination of Mycobacterium tuberculosis complex (MTC) from non-tuberculous mycobacteria (NTM), a novel, simple, and primer-combined single-step multiplex PCR using three primer pairs (6110F-6110R, 1081F-1081R, and 23SF-23SR; annealing on each of IS6110, IS1081, and 23S rDNA targets), hereafter referred to as a triplex PCR, has been developed and evaluated. The expected product for IS6110 is 416 bp, for IS1081 is 300 bp, and for 23S rDNA is 206 bp by single PCR, which was used to verify the specificity of primers and the identity of MTC using DNA extracted from the M. tuberculosis H37Rv reference strain (ATCC, USA) and other mycobacteria other than tuberculosis (MOTT) templates. The triplex PCR assay showed 100% specificity and 96% sensitivity; the limit of detection for mycobacteria was ~100 fg; and it failed to amplify any target from DNA of MOTT (50 samples tested). Of 307 blinded clinical samples, overall 205 positive M. tuberculosis samples were detected by single PCR, 142 by conventional culture, and 90 by AFB smear methods. Remarkably, the triplex PCR could subsequently detect 55 positive M. tuberculosis from 165 culture-negative and 115 from 217 AFB smear-negative samples. The triplex PCR, targeting three regions in the M. tuberculosis genome, has proved to be an efficient tool for increasing positive detection/discrimination of this bacterium from clinical samples.

• The Plant Pathology Journal
• /
• v.31 no.2
• /
• pp.123-131
• /
• 2015

Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.