• Fisheries and Aquatic Sciences
• /
• v.5 no.1
• /
• pp.75-78
• /
• 2002

The effects of Coehlodinium polykrikoides on the gill of red sea bream, Pagrus major, were examined to clarify the ichthyotoxic mechanisms of this plankton species. The gill of fish exposed to dense blooms over 3,000 cells/mL for 24 h showed severe epithelial separation: a severe edema was found in the secondary lamellar epithelium and interlamellar regions of primary filament. In addition, lipid peroxidation of gill tissue in Coehlodinium-exposed fish was about 2.5 times higher than that of control. The composition of glycoproteins in the gill mucus of Coehlodinium-exposed fish was also changed. These results suggest that the loss of structural integrity of cell membranes in fish gill may be deeply involved in fish death by C. polykrikoides.

• Fisheries and Aquatic Sciences
• /
• v.5 no.1
• /
• pp.54-58
• /
• 2002

The polymerase chain reaction (PCR) amplification technique was used for comparison of Listeria monocytogenes serotypes. PCR primers for the fragment of invasion-associated protein (iap) gene were highly specific for all the serotypes of L. monocytogenes. Other Listeria spp., such as Listeria ivanovii and Listeria innocua were not produced the PCR fragments by above primer set. The nucleotide sequences of PCR products showed high homologies in comparison of all the isolated serotypes except unknown type II-2. The deduced amino acid sequences of the PCR products also showed similar to one another. The various region of the PCR products, called a Thr-Asn repeat region was presented. All of isolated L. monocytogenes serotypes possessed 16 to 20 Thr-Asn repeats.

• Fisheries and Aquatic Sciences
• /
• v.5 no.1
• /
• pp.32-35
• /
• 2002

In this study, we investigated the mechanism of cell death in rhabdovirus-infected cells, chinook salmon embryonic cell line (CHSE-2l4) infected with hirame rhabdovirus (HIRRV). Studies using light microscopy, fluorescence microscopy, TUNEL method, electron microscopy, and agarose gel electrophoresis revealed changes in the cell morphology and DNA fragmentation indicative of apoptosis in early infection. It was observed that HIRRV induced apoptosis as well as necrosis in infected cells.

• Fisheries and Aquatic Sciences
• /
• v.5 no.1
• /
• pp.21-27
• /
• 2002

Angiotensin I converting enzyme inhibitory activities of shelled krill (Euphausia superba) hydrolysates by autolysis and by hydrolysis with commercial proteases were analyzed. Among the proteases, Alcalase was the most effective protease for the hydrolysis of krill considering the degree of hydrolysis $(87.5\%)$ and the ACE inhibitory activity $(60\%)$. Four hour hydrolysis suggested as the most suitable and economic. In order to establish the optimum hydrolysis condition of krill, degree of hydrolysis and ACE inhibitory activity as affected by Alcalase concentration and water amount added were statistically analyzed by response surface methodology (RSM). The optimum hydrolysis condition was $2.0\%$ Alcalase hydrolysis in 2 volumes (v/w) of water at $55\% for 4 hr. The hydrolysate prepared from the optimum hydrolysis condition was fractionated by molecular weight. The lower molecular weight fraction showed the higher ACE inhibitory activity. $IC_{50}$ of the fraction under 500 Da was 0.57mg protein/mL.

• Fisheries and Aquatic Sciences
• /
• v.9 no.2
• /
• pp.91-96
• /
• 2006

The movement range and diurnal behavior of 17 wild black rockfish (Sebastes schlegeli) were monitored within the marine ranching area of Tongyeong from 27 July to 7 November, 2005 using acoustic telemetry. Coded transmitters were surgically implanted into the abdomen of fish. Two of ten fish released in summer moved about 2 km away from the capture point for 1 day. The others stayed within a 500-m radius of the release point for 1 week after release. The seven fish released in autumn stayed within 500 m of the release point. Most fish were more active and had a greater range of horizontal diurnal movement at night than during the day. Additionally, the range of horizontal diurnal variation was greater in summer than in autumn. While fish released in summer were more vertically active at night than during the day, those released in autumn preferred to move vertically during the day.

• Fisheries and Aquatic Sciences
• /
• v.9 no.3
• /
• pp.123-128
• /
• 2006

An epizootic causing mortality among cultured marine finfishes occurred in 1999 in the province of Kyoungsang, Korea. The disease was characterized by the presence of enterocele, abdominal swelling, and gastroenteritis. The causative bacteria were isolated from olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), turbot (Scophthalmus maximus) and the rearing water. These bacteria showed swarming activity on agar plates and yellowish or greenish colonies on thiosulfate-citrate-bile salts-sucrose (TCBS) agar plates, but no luminescence. The pathogen was identified as Vibrio harveyi based on morphological and biochemical characteristics and the sequence of l6S rDNA. The lethal doses (LD$_{50}$) of olive flounder and black rockfish were estimated to be $1.24\times10^6-1.36\times10^8$ and $3.24\times10^5-5.8\times10^7$ CFU/fish respectively following intraperitoneal injection.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.27 no.1
• /
• pp.135-140
• /
• 2003

The convergence of finite volume method (FVM) or discrete ordinate method (DOM) is known to degrade for optical thickness greater than unity and large scattering albedo. The present article presents a convergence acceleration procedure for the FVM based on a full approximation storage (FAS) multigrid method. Among a variety of multigrid cycles, the V-cycle is used and the full multigrid algorithm (FMG) is applied to an analysis of radiation in irregular two-dimensional geometry. Solution convergence is discussed for the several cases of various optical thickness and scattering albedo. At small scattering albedo and optical thickness, there is no advantage to using the multigrid method for calculation CPU time. For large scattering albedo greater than 0.5 and optical thickness greater than unity, however, the multigrid method improves the convergence and the solution is rapidly obtained.

• The Korean Journal of Financial Studies
• /
• v.4 no.1
• /
• pp.233-258
• /
• 1998

본 연구는 비정상성과 관련된 기존의 연구들이 베타위험과 추가설명변수들 사이의 상관관계를 무시한 검증방법의 오류를 지적한다. 그 상관관계를 고려한 포트폴리오 구성방법을 선택한 후, 비정상성에 관한 좀 더 정밀한 검증을 실시하여 규모효과와 EP효과를 검토한다. 더 나아가 CAPM의 두 가지 주장, 즉, 베타위험의 유일성과 이 위험과 그 자산의 수익률간의 양의 상관관계를 갖는다는 두 가지 주장을 세밀히 검토하게 된다. 또한 이 모든 검증에서 등가중지수(Equal-Weighted Index: EWI)와 가치가중지수(Value-Weighted Index: VWI)의 적용에 대한 차이도 동시에 검토하였다. 1980년 1월부터 1993년 6월까지의 월별수익률과 한국신용평가주식회사의 주식수익률 데이터베이스(KIS-SMAT), 재무제표 데이터베이스(KIS-FAS)를 이용하여 검증한 결과, 한국중권시장에서는 규모효과만 존재하였고, EP효과가 존재한다는 증거는 얻지 못하였다. 또한 EWI와 VWI의 지수에 대한 차이는 아주 미미하였다. 특히 CAPM에서 주장하는 베타위험의 유일성과 베타위험과 그 자산의 수익률의 양의 상관관계에 대한 두 가지 사실에 대해서는 오히려 상반된 증거만을 확인할 수 있었다.

• Fisheries and Aquatic Sciences
• /
• v.5 no.4
• /
• pp.258-262
• /
• 2002

Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

• Fisheries and Aquatic Sciences
• /
• v.6 no.4
• /
• pp.225-227
• /
• 2003

Acetylcholinesterase (AChE) activity is a potential biomarker for phenanthrene exposure in aquatic organisms. Olive flounder (Paralichthys olivaceus) were exposed to three different concentrations (0.5, 1.0 and 2.0, uM) of phenanthrene for four weeks. AChE activities in the brain, heart and eyes were documented. Inhibition of AChE activity was found significant in flounder treated with a concentration greater than $1.0 {\mu}M$ of phenanthrene. This indicates that a chronic exposure to phenanthrene induces damage in various organs (brain, heart and eyes) and changes of AChE activities might be a useful biomarker to assess the impacts induced by polycyclic aromatic hydrocarbon (PAH). Evidence from this study confirms that the measurement of AChE in the brain and eyes of flounder is a valuable tool that along with other biomarkers can maximize an ecotoxicologists' confidence in assessing the impacts of oil and PAH pollution in the aquatic environment.