Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Angiotensin I Converting Enzyme Inhibitory Activity of Krill (Euphausia superba) Hydrolysate

  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Angiotensin I converting enzyme inhibitory activities of shelled krill (Euphausia superba) hydrolysates by autolysis and by hydrolysis with commercial proteases were analyzed. Among the proteases, Alcalase was the most effective protease for the hydrolysis of krill considering the degree of hydrolysis $(87.5\%)$ and the ACE inhibitory activity $(60\%)$. Four hour hydrolysis suggested as the most suitable and economic. In order to establish the optimum hydrolysis condition of krill, degree of hydrolysis and ACE inhibitory activity as affected by Alcalase concentration and water amount added were statistically analyzed by response surface methodology (RSM). The optimum hydrolysis condition was $2.0\%$ Alcalase hydrolysis in 2 volumes (v/w) of water at $55\% for 4 hr. The hydrolysate prepared from the optimum hydrolysis condition was fractionated by molecular weight. The lower molecular weight fraction showed the higher ACE inhibitory activity. $IC_{50}$ of the fraction under 500 Da was 0.57mg protein/mL.

Keywords

References

  1. Cushman, D.W. and H.S. Cheung. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzy-me of rabbit lung. Biochemical Pharmacology, 20, 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  2. Do, J.R. 2000. Separation and puriflcation of angiotensin-I converting enzyme inhibitory peptide from Mackerel. J. Korean Fish. Soc., 33, 153-157
  3. Garbers, D.L. and S.K. Dubois. 1999. The Molecular basis of hypertension. Annu. Rev. Biochem., 68, 127-55 https://doi.org/10.1146/annurev.biochem.68.1.127
  4. Kim, Y.H., H.G. Lee, J.R. Do, H.K. Shin and C.B. Yang. 2000. Purification and identification of angiotensin-I converting enzyme inhibitory peptide from Turban Shell (Turbo corntus).Food Sci. Biotechnol., 9, 353-357
  5. Kohama, Y., S. Matsumoto, H. Oka, T. Teramoto, M. Okabe and T. Mimura. 1988. Isolation of angiotensin-convertng enzyme inhibitor from tuna muscle. Biochem. Biophys. Res. Commun., 155, 332-337 https://doi.org/10.1016/S0006-291X(88)81089-1
  6. Lee, T.G., Y.B. Park, D.C. Park, D.M. Yeum, I.S. Kim, Y.S. Gu, Y.H. Park and S.B. Kim. 1998. Angiotensin Conver-ting enzyme inhibitory activity in enzymatic hydrolysates of anchovy muscle protein. J. Korean Fish. Soc., 31, 875-881
  7. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with the fblin phenol reagent. J. Biol. Chem., 193, 265-275
  8. Matsui, T., H. Matsufuji, E. Seki, K. Osajima, M. Nakashima and Y. Osajima. 1993. Inhibition of angiotessin I -converting enzyme by Bacitlus lichemformis alkaline Protease hydrolysates derived from sardine muscle. Biosci.Biotech. Biochem., 57, 922-925 https://doi.org/10.1271/bbb.57.922
  9. Oh, S.J., S.H. Kim, S.K. Kim, Y.J. Baek, and K.H. Cho. 1997 Angiotensin-I converting enzyme inhibitory activity ofthe K-casein fragments hydrolysated by chymosin, Pepsin and trypsin. Korean J. Food Sci. TechnoL, 29, 1316-1318
  10. Ondetti, M.A. and D.W. Cushman. 1982. Enzymes of the renin-angiotensin system and their inhibitors. Ann. Rev. Biochem., 51, 283-308 https://doi.org/10.1146/annurev.bi.51.070182.001435
  11. Ross, R.M. and L.B. Quetin. 1986. How productive are Antarctic krill ? Bioscience, 36, 264-269 https://doi.org/10.2307/1310217
  12. Suetsuna, K. 1998. Separation and identification of angiotensin I -converting enzyme inhibitory peptides from peptic digest of Hizikia fusiformis protein. Nippon Suisan Gakkaishi., 64, 862-866 https://doi.org/10.2331/suisan.64.862
  13. Watanabe, T., K. Sugii, H. Yuguchi and T. Kinumaki, 1976. Studies on the utilization of antarctic krill, Epbausia superba Dana-II. Bull. Tokai Reg. Fish. Res. Lab., 13-30
  14. Yamamoto, S., I. Toida and K. Iwai. 1980. Re-examination of the spectrophotomethc assay for serum angiotensin-converting enzyme. Japan. J. Thoracic Disease, 18, 297~303 (in Japanese)

Cited by

  1. Radical Scavenging Potential of Hydrophilic Phlorotannins of Hizikia fusiformis vol.20, pp.1, 2002, https://doi.org/10.4490/algae.2005.20.1.069
  2. Screening of Extracts from Red Algae in Jeju for Potentials MarineAngiotensin - I Converting Enzyme (ACE) Inhibitory Activity vol.21, pp.3, 2002, https://doi.org/10.4490/algae.2006.21.3.343
  3. 효소분해 진주조개(Pinctada fucata martensii) 젓갈의 제조 및 품질특성 vol.39, pp.1, 2006, https://doi.org/10.5657/kfas.2006.39.1.009