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Structure Analysis of pmcABCDEFT Gene Cluster for Degradation of Protocatechuate from Comamonas sp. Strain DJ-12 (Comamonas sp. Strain DJ-12로부터 Protocatechuate의 분해에 관여하는 pmcABCDEFT 유전자군의 구조 분석)

  • Kang Cheol-Hee;Lee Sang-Mhan;Lee Kyoung;Lee Dong-Hun;Kim Chi-Kyung
    • Korean Journal of Microbiology
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    • v.41 no.3
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    • pp.195-200
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    • 2005
  • Comamonas sp. strain DJ-12 is a bacterial isolate capable of degrading of 4-chlorobiphenyl (4CB) as a carbon and energy source. The degradation pathway was characterized as being conducted by consecutive reactions of the meta-degradation of 4CB, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, and meta-degradation of protocatechuate to product TCA metabolites. The 6.8 kb fragment from the chromosomal DNA of Comamonas sp. strain DJ-12 included the genes encoding for the meta-degradation of PCA; the genes of protocatechuate 4,5-dioxygenase alpha and beta subunits (pmcA and pmcB), 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (pmcC), 2-pyrone-4,6-dicarboxylate hydrolase (pmcD), 4-oxalomesaconate (OMA) hydratase(pmcE), 4-oxalocitramalate (OCM) aldolase (pmcF), and transporter gene (pmcT). They were organized in the order of pmcT-pmcE-pmcF-pmcD-pmcA-pmcB-pmcC. The amino acid sequences deduced from the nucleotide sequences of pmcABCDEFT genes from Comamonas sp. strain DJ-12 exhibited 94 to $98\%$ homologies with those of Comamonas testosteroni BR6020 and Pseudomonas ochraceae NGJ1, but only 52 to $74\%$ with homologies Sphingomonas paucimobilis SYK-6, Sphingomonas sp. LB126, and Arthrobacter keyseri 12B.

A Study on the Physical Properties of Addition Silicone Impression Materials (부가중합형실리콘인상재의 물리적 특성에 대한 연구)

  • Kim, Soo-Hwa
    • Journal of dental hygiene science
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    • v.6 no.4
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    • pp.231-235
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    • 2006
  • This study evaluated the properties of polyvinylsiloxane impression materials: Strain-in-compression, Elastic recovery, Consistency, and Wettability. Four polyvinylsiloxane impression materials(Examixfine, GC, Japan; Aquasilultra, Dentsply, USA; Extrude, Kerr, USA; Perfect-F, Handae, Korea) were used in this study. The measured data were analyzed with SPSSWIN 12.0 program. The results were as follows : 1. The strain-in-compression values showed that Extrude had the lowest value, 4.86% and Examixfine had the highest value, 6.78%. Significant difference between Examixfine and Perfect-F was not found but the other groups had significant differences(p = 0.000). 2. In the elastic recovery test, Extrude had the highest, 99.83% and Perfect-F had the lowest, 96.54%. There is no significant difference between Examixfine and Aquasilultra. The other impression material groups showed significant differences(p = 0.000). 3. The measurement of consistency results that the diameter of Examixfine was the largest, 45.12mm and one of Perfect-F was the smallest, 40.28 mm. No differences were shown between Examixfine and Aquasilultra and between Extrude and Perfect-F(p = 0.001). 4. Aquasilultra had a better hydrophilicity($47.85^{\circ}$) than other materials. Perfect-F had a larger contact angle($94.89^{\circ}$) and was hydrophobic. There were significant differences between groups(p = 0.000). 5. As for correlation among the test types, contact angle correlated with strain-in-compression, elstic recovery and consistency. The strain-in-compression had correlation to contact angle and elastic recovery. The elastic recovery correlated with contact angle and strain-in-compression. And the consistency had correlation to contact angle.

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Effect of Defect Energy levels on the AC PDP Discharging Characteristics (MgO 보호막의 결함 전위 레벨이 AC-PDP 방전 특성에 미치는 효과)

  • Kwon, Sang-Jik;Kim, Yong-Jae;Cho, Eou-Sik
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.12
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    • pp.12-17
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    • 2007
  • The effects of the evaporation rate of MgO films using an electron beam on the MgO properties and the discharge characteristics of a plasma display panel(PDP) were investigated and analyzed. Mgo films were deposited with the various MgO evaporation rates. The MgO properties such as the crystal orientation, the surface roughness, and the film structure, were inspected using XRD(X-ray diffractometry), AFM(atomic force microscopy). From the experiments and Paschen law, the maximum value of the secondary, electron emission coefficient $(\gamma)$ was obtained at the evaporation rate of $5\AA/sec$. The minimum firing voltage and the maximum luminous efficiency were obtained at an evaporation rate of $5\AA/sec$. In the MgO film deposited at $5\AA/sec$, the (200) orientation and $F^+$ center were most intensive. The XRD results and cathode-luminescence(CL) spectra show the $\gamma$ values are correlated with $F/F^+$ centers of the molecular structure of MgO films.

Minimally Invasive Transforaminal Lumbar Interbody Fusion Using a Single Interbody Cage and a Tubular Retraction System : Technical Tips, and Perioperative, Radiologic and Clinical Outcomes

  • Lee, Chang-Kyu;Park, Jeong-Yoon;Zhang, Ho-Yeol
    • Journal of Korean Neurosurgical Society
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    • v.48 no.3
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    • pp.219-224
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    • 2010
  • Objective : A minimally invasive transforaminal lumbar interbody fusion (MIS TLlF) has recently been introduced. However, MIS TLlF is a technically challenging procedure. The authors performed retrospective analysis about MIS TLlF using a single interbody cage. Methods : Twenty-eight consecutive patients were treated by MIS TLlF. Of these 28 patients, 20 patients were included in this retrospective study. Perioperative, clinical, and radiologic outcomes were assessed. Clinical outcomes were assessed using Oswestry Disability Index (ODI) and Visual Analogue Scores (VAS). Fusion rates and cross-sections of operated spinal canals were assessed by CT. Results : Twelve patients underwent MIS TLlF at one segment and 8 patients at two segments (L3/4: 4, L4/5: 17, L5/S1: 7). Operation time for a single segment was 131.7 min and for two segment was 201.4 min, and corresponding blood losses were 208.3 mL and 481.2 mL, respectively. ODI and VAS scores were significantly improved at 6 months postop (ODI from 30.32 to 15.54, VAS from 7.80 to 2.20, p = 0.001) Twenty-two segments (78.6%) achieved grade I fusion, 4 segments (14.3%) achieved grade II, 2 segments (7.1%) achieved grade III and 0 segments achieved grade IV at 12 months. Postoperatively at 12 months, spinal canal cross sectional areas at disc spaces significantly increased from 157.5 to $294.3\;mm^2$ (p = 0.012). Conclusion : MIS TLlF achieved good clinical outcomes and high fusion rates. Our findings show that MIS TLlF performed with a single Interbody cage and a tubular retractor system can be used as a standard MIS TLlF technique.

Silicon Capacitive Pressure Sensor for Low Pressure Measurements (저 압력 측정을 위한 실리콘 용량형 압력센서)

  • Seo, Hee-Don;Lee, Youn-Hee;Park, Jong-Dae;Choi, Se-Gon
    • Journal of Sensor Science and Technology
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    • v.2 no.1
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    • pp.19-27
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    • 1993
  • Capacitive pressure sensor for low pressure measurements has been fabricated by using $n^{+}$ epitaxial layer electrochemical etching stop and glass-to-silicon electrostatic bonding technique. The sensor had hybrid configuration of a sensor chip, which consists of sensor capacitor and reference capacitor, and two output signal detection IC chips. A fabricated sensor, with a $1.0{\times}1.0 mm^{2}$ square size and a $10{\mu}m$ thick flat diaphragm, showed a 7.1 pF zero pressure capacitance, and 5.2 % F.S, sensitivity in 10 KPa pressure range. By using a capacitance to voltage converter, the thermal zero shift of 0.051 %F.S./$^{\circ}C$ and the thermal sensitivity shift of 0.12 %F.S./$^{\circ}C$ for temperature range of $5{\sim}45^{\circ}C$ were obtained.

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KINEMATIC STRUCTURES OF CERTAIN LOOPS

  • Im, Bok-Hee
    • Communications of the Korean Mathematical Society
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    • v.12 no.3
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    • pp.543-551
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    • 1997
  • In this paper, we call a loop F kinematic if for $a, b \in F\{0}$, the following two conditions are valid : (i) the centralizer Z(a) of a is a commutative group under the induced operation from the loop F, and (ii) Z(a) = Z(b) or $Z(a) \cap Z(b) = {0}$, where 0 is the identity of F. Some example of kinematic loops are given.

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ON THE BEREZIN TRANSFORM ON $D^n$

  • Lee, Jae-Sung
    • Communications of the Korean Mathematical Society
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    • v.12 no.2
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    • pp.311-324
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    • 1997
  • We show that if $f \in L^{\infty}(D^n)$ satisfies Sf = rf for some r in the unit circle, where S is any convex combination of the iterations of Berezin operator, then f is n-harmonic. And we give some remarks and a conjecture on the space $M_2={f \in L^2(D^2, m \times m)\midBf = f$.

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Fusarium spp. Isolated from Seed, Root and Cultivated Soil of Phaseouls vidissimus and Their Pathogenicity (녹두종자, 뿌리와 녹두 재배토양에서 분리된 Fusarium spp.와 병원성)

  • Paik Su Bong;Do Eun Su
    • Korean Journal Plant Pathology
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    • v.3 no.1
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    • pp.8-12
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    • 1987
  • Fusarium oxysporum, F. moniliforme, F. solani, F. equiseti, F. semitectum, and F. sporotrichioides were detected from seeds, roots and cultivated soil of Phaseolous vidissimus collected from Kyung-gi Provincial Rural Development Administration. The rate of seedling desease incidence was $60\%$ by testing of seed germination using a large petri-dish. According to the blotter method, F. moniliforme showed $7\%$ infection at seed-coat and $2\%$ at cotyledon and embryo. Their pathogenicities of F. moniliforme, F. semitectum, F. equiseti, and F. sporotrichioides isolated from seeds were recognized on seedlings by water-agar test tube methods. F. oxysporum and F. solani isolated from infected-roots had their pathogenicity by water-agar test tube method but were weakly pathogenic by soil treatment method. Their pathogenicities of F. oxysporum. F. solani and F. uiseti isolated from cultivated-soil were recognized by water-agar test tube method. These F. oxysporum and equiseti isolates had their pathogenicities but F. solani was weakly pathogenic by soil treatment method.

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Comparative Height Growth and Forest Structure of Fraxinus Spaethiana and Pterocarya Rhoifolia in Natural Reforestation Stands in Steep Valleys of Central Japan

  • Ann, Seoung-Won
    • Journal of Environmental Science International
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    • v.15 no.12
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    • pp.1119-1124
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    • 2006
  • Height-growth analysis was used to examine forest structure and compare Pterocarya rhoifolia and Fraxinus spaethiana growth characteristics within and between each species in two P. rhoifolia-dominant and two F. spaethiana established contemporaneously in the sere, species vertical stratification 25 years after stand initiation was such that P. rhoifolia dominated the overstory but F. spaethiana the understory, including that P. rhoifolia grew about 4 times more rapidly Similarly, F. spaethiana dominated the overstory but not the understory, in a stand where it established mainly by itself, 25 years after Initiation. However, comparing the two different stands, P. rhoifolia overstory heights were about two times greater than F. spaethiana. This suggests that in a disturbance regime, forest regeneration is affected by height-growth patterns such that P. rhoifolia's ability to achieverapid height growth allows it to dominate where lights growth allows it to dominate where light resources are continuously abundant.