• 한국가축번식학회지
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• 제27권4호
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• pp.317-324
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• 2003

The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F$_{0}$). The first generation of transgenic pig (F$_{0}$) harboring mWAP-hEPO appeared to be a male, and the second generation (F$_1$) pigs were made by natural mating of F$_{0}$ with domestic swine, and male and female transgenic pigs (F$_1$) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.earch.

• 전자공학회논문지CI
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• 제41권4호
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• pp.67-72
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• 2004

본 논문에서는 네트워크기반의 클라이언트-서버모델에서 병렬유전자알고리즘의 최적해 수렴속도를 향상시키는 방법을 제안한다. 전역 최적해를 지역 엘리트의 평가만으로 구하는 기존의 방법과는 달리 제안한 방법은 서버에서 지역 엘리트의 평가를 통해 전역 최적해를 구하고 유휴시간에 유전자알고리즘을 적용하여 전역 최적해의 적합도를 개선한다. 서버에서 개선된 전역 최적해를 클라이언트의 유전자알고리즘에서 사용하므로 전체 알고리즘의 최적해 수렴속도가 향상된다 Fmax(g)는 g번째 세대의 최대 적합도, G는 서버에서 개선되는 세대수일 때, 지역 최적해의 이주 시 서버에서 개선되는 적합도는 (equation omitted)(F/sub max/(g)-F/sub max/(g-1)) 이다. 여기서 클라이언트의 수가 증가하면 G가 작아져서 적합도 개선치는 줄어드나 기존의 방법보다 적합도가 개선됨을 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1524-1528
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• 2005

Molecular genetic markers were used to detect chromosomal regions which contain economically important traits such as growth, carcass, and meat quality traits in pigs. A three generation resource population was constructed from a cross between Korean native boars and Landrace sows. A total of 240 F2 animals from intercross of F1 was produced. Phenotypic data on 17 traits, birth weight, body weights at 3, 5, 12, and 30 weeks of age, teat number, carcass weight, backfat thickness, body fat, backbone number, muscle pH, meat color, drip loss, cooking loss, water holding capacity, shear force, and intramuscular fat content were collected for F2 animals. Animals including grandparents (F0), parents (F1), and offspring (F2) were genotyped for 80 microsatellite markers covering from chromosome 1 to 10. Least squares regression interval mapping was used for quantitative trait loci (QTL) identification. Significance thresholds were determined by permutation tests. A total of 10 QTL were detected at 5% chromosome-wide significance levels for growth traits on SSCs 2, 4, 5, 6, and 8.

• 한국연초학회지
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• 제20권1호
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• pp.33-39
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• 1998

Survival, growth and reproduction of the cigarette beetle, Lasiodema sewicome F., were studied on flue-cured (NC82), air-cured (Br.21), aromatic (Izmir) tobacco and artificial diet (wheat feed 95% + yeast 5%). Cigarette beetle adults occurred from first generation in the flue-cured and aromatic tobacco, but no adults emerged in air-cured tobacco after the 2nd generations. The head capsule widths of larvae of the cigarette beetles L sewicome F.1 reared on different diets at 2$0^{\circ}C$, $25^{\circ}C$ and 3$0^{\circ}C$ were measured. The head capsule development was slower at 2$0^{\circ}C$ on the same diets than $25^{\circ}C$ and 3$0^{\circ}C$, and slower in the flue-cured and aromatic tobacco than the artificial diet at 2$0^{\circ}C$ and 2S$^{\circ}C$, At $25^{\circ}C$ the larval head capsule developed more rapidly in the aromatic tobacco than the flue-cured tobacco. However, no larval head development was noticed in the air-cured tobacco. The correlation between population densities of cigarette beetle,1 sewicome F., and sugar content of tobacco leaves was positive, but negative in nicotine contents. The regression between densities and sugar content was Y : 22.79 X + 57.29 (r=0.891**), and between nicotine content was Y : -141.31X + 321.40(r=-0.917**).

• 한국연초학회지
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• 제22권1호
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• pp.25-30
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• 2000

Bacterial wilt(Ralstonia solanacearum) is one of the major diseases of flue-cured tobacco (Nicotiana tabacum L.) in the world. This study was conducted to investigate degree of dominance, selection, and correlation between leaf shape and degree of bacterial wilt resistance in flue-cured tobacco. The degree of disease caused by bacterial wilt was evaluated in parents, F$_1$, F$_2$ and F$_3$ populations of two crosses, BY 4 x NC 95 and BY 4 x Coker 86, in the infected field. The leaf shape index was also measured in parents and F$_2$ population of BY 4 x NC 95. The incidence of bacterial wilt was observed in the middle of June and peaked in late July, when the highest value of pathogen density reached 1.0 x 10$^{6}$ colony forming unit per gram. It was concluded that the inheritance mode of risestance to bacterial wilt in the above two crosses of susceptible and resistant varieties was recessive and polygenic. The resistance to bacterial wilt was significantly correlated with leaf shape in F2 generation of BY 4 x NC 95. But certain plants having narrower leaves were also resistant to bacterial wilt. It is considered that the bacterial wilt resistant lines having narrower leaves could be selected. The selection for bacterial wilt resistance in the F$_2$ population might be effective.

• 한국육종학회지
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• 제41권4호
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• pp.463-467
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• 2009

Allium 속 근연종인 파와 양파간 종간교잡을 이용하여 양파로부터 새로운 형질을 도입하는 과정에서 유래되는 종간교잡 F1과 여교잡 세대들의 게놈크기의 변화를 flow cytometry를 이용하여 측정한 결과 다음과 같은 결과를 얻었다. 1. 양파와 파의 종간교잡 F1의 식물학적 특성은 양친의 중간형을 보였으나 여교잡이 진전됨에 따라 반복친인 파의 표현형이 우세하였다. flow cytometry를 이용하여 2C nuclear DNA content을 측정한 결과 $F_1$은 양친에 비해 오히려 증가하였으나 여교잡이 진행됨에 따라 급속히 감소하여 $BC_2F_1$에서는 반복친과 비슷한 수준으로 감소하였다. 여교잡세대의 자식인 $BC_1F_2$은 반복친과 비슷한 $23.1{\pm}1.0pg$으로 측정되었으나 $BC_2F_2$는 $22.9{\pm}0.4pg$으로 반복친의 2C nuclear DNA content인 $23.2{\pm}0.2pg$ 보다 오히려 감소하였다.

• 농업생명과학연구
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• 제46권6호
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• pp.1-8
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• 2012

본 시험은 한국 고추 육종에 이용되는 교배친의 고추맛에 관여하는 유전자의 유전가를 구명하기 위해 수행하였다. 고추맛과 원예적 특성이 상이한 '#1803'($P_1$)과 '#1532'($P_2$) 두 교배친을 교배하여 이들의 $F_1$, $F_2$ 세대를 본 시험의 공시 재료로 이용하였다. Partitioning method에 따라 고추맛은 A-a와 B-b 두 유효 유전자에 의하여 지배되는데 $F_1$ 세대는 우수 교배친 $P_1$보다 더 우수한 초우성 현상과 $F_2$ 세대는 초월 분리가 일부 나타났다. 고추맛의 유효 유전자 효과는 A-a 유전자에 의해 0.36, B-b 유전자에 의해 0.64가 증가하였다. 한국 고추맛은 유효 유전자간에서 0.94의 간섭효과와 최대 2.86의 제 2효과가 관여하여 복합적인 유전양식을 나타내었다.

• 말소리와 음성과학
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• 제4권4호
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• pp.29-35
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• 2012

The purpose of this study was to measure the first two vowel formants of the forty male and female speakers (twenty young vs. old male speakers and twenty young vs. old female speakers) from the Buckeye Corpus of Conversational Speech and to examine the vowel formant changes across two generations (younger vs. older). The results indicated that the vowel space of the younger generation (in their thirties or less) shifted to the lower left position compared to those of the older generation (in their forties or more) in both male and female speakers. When the results were compared to those of Peterson & Barney (1952), it appears that differences can be found in the size of the vowel spaces through time.

• 대한음성학회지:말소리
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• 제57호
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• pp.15-30
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• 2006

This paper investigates differences between generations in the vowel system of the Daegu dialect in terms of F1 and F2 of the monophthongs. Three different groups of subjects participated in the present study: 20 female native speakers of the Daegu dialect(10 in their 20's and 10 in their 40's), and 10 female native speakers of the Seoul dialect as a control group. It has been assumed that the Daegu dialect has six vowels. However, younger generation appears to have 7 vowels different from older generation. The result of the present study showed that the Daegu dialect has different vowel systems between generations: for 40's have six vowels and 20's have seven vowels. These differences seems to be attributed to the influence of the Seoul dialect.

• Biomolecules & Therapeutics
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• 제24권1호
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• pp.49-56
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• 2016

To determine the protective effect of aloe-emodin (AE) from high glucose induced toxicity in RIN-5F (pancreatic ${\beta}$-cell) cell and restoration of its function was analyzed. RIN-5F cells have been cultured in high glucose (25 mM glucose) condition, with and without AE treatment. RIN-5F cells cultured in high glucose decreased cell viability and increased ROS levels after 48 hr compared with standard medium (5.5 mM glucose). Glucotoxicity was confirmed by significantly increased ROS production, increased pro-inflammatory (IFN-${\gamma}$, IL-$1{\beta}$,) & decreased anti-inflammatory (IL-6&IL-10) cytokine levels, increased DNA fragmentation. In addition, we found increased Bax, caspase 3, Fadd, and Fas and significantly reduced Bcl-2 expression after 48 hr. RIN-5F treated with both high glucose and AE ($20{\mu}M$) decreased ROS generation and prevent RIN-5F cell from glucotoxicity. In addition, AE treated cells cultured in high glucose were transferred to standard medium, normal responsiveness to glucose was restored within 8hr and normal basal insulin release within 24 hr was achieved when compared to high glucose.