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A Duplex PCR for Detection of Phytophthora katsurae Causing Chestnut Ink Disease (밤나무 잉크병균, Phytophthora katsurae의 검출을 위한 Duplex PCR)

  • Lee, Dong-Hyeon;Lee, Sun-Keun;Kim, Hye-Jeong;Lee, Sang-Hyun;Lee, Sang-Yong;Lee, Jong-Kyu
    • Research in Plant Disease
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    • v.18 no.2
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    • pp.73-79
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    • 2012
  • Phytophthora katsurae is a fungal pathogen responsible for chestnut ink disease. We designed two duplex primer sets (SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R) to detect P. katsurae. SOPC 1F/1R and SOPC 1-1F/1-1R primer pairs were designed for sequence characteristic amplification regions (SCAR) marker, and KatI 3F/5R primer pair was used for P. katsurae-specific primer designed from internal transcribed spacer (ITS) region. To assess the sensitivity of duplex PCR, genomic DNA was serially diluted 10-fold to make the final concentrations from 1 mg/ml to 1 ng/ml. The sensitivity for two primer sets were 1 ${\mu}g/ml$ and 100 ng/ml, respectively. To find detection limits for zoospores of P. katsurae, each zoospore suspension was serially diluted 10-fold to make the final concentrations from $1{\times}10^6$ to $1{\times}10^2$ cells/ml, and then DNA was extracted. The limits of detection for all of two primer sets were $1{\times}10^5$ cells/ml. All of two primer sets were specific to P. katsurae in PCR detection and did not produce any P. katsurae-specific PCR amplicons from other 16 Phytophthora species used as the control. This study shows that duplex PCR using two primer sets might be a useful tool for rapid and efficient detection of P. katsurae.

The Effects of Prostaglandin $F_{2\alpha}(PGF_{2\alpha})$ Treatment on Hormone Concentrations and Follicular Development in Early Postpartum Korean Native Goats (한국 재래 산양에 있어서 Prostaglandin $F_{2\alpha}(PGF_{2\alpha})$의 투여가 호르몬 함량 및 난포의 발육에 미치는 영향)

  • 변명대;함태수
    • Korean Journal of Animal Reproduction
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    • v.25 no.2
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    • pp.155-162
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    • 2001
  • These experiments were conducted to evaluate the effects of the administration of exogenous PG $F_{2{\alpha}}$ on hormone concentrations and follicular development in early postpartum(pp) Korean native goats. 1. Plasma PG $F_{2{\alpha}}$ concentrations in PG $F_{2{\alpha}}$ treated goals showed a gradual increase from a low on day 2 (GR-1 : 10 day-treatment group: 6.35$\pm$0.5 and GR-2; 4 day-treatment group: 0.22$\pm$0.2 pg/$m\ell$, respectively) to reach a peak of 21.18$\pm$1.6 or 4.21 $\pm$0.4 pg/$m\ell$ on day 4 after treatment of PG $F_{2{\alpha}}$. 2. Plasma PG $F_{2{\alpha}}$ concentrations in GR-1 goats averaged 9.08 $\pm$1.2 pg/$m\ell$ compared with 5.44$\pm$ 1.8 pg/$m\ell$ in GR-2 goats the day before treatment of PG $F_{2{\alpha}}$. Mean PG $F_{2{\alpha}}$ concentrations thereafter remained low during the treatment period but PG $F_{2{\alpha}}$ concentrations did not differ between the two group. 3. Plasma concentrations of estradiol-17 $\beta$ (E,) in PG $F_{2{\alpha}}$ - treated group were decreased gradually until 2 days after PG $F_{2{\alpha}}$ treatment but mean $E_2$ concentrations began to increase on 3 days and were Inaximal on the 4 days after treatment. 4. Plasma lulenizing hormone (LH) concentrations in PG $F_{2{\alpha}}$ - treated goats were slightly higher than in controls but mean LH concentrations did not differ between the two treatment groups. 5. Plasma prolactin (PRL) concentrations were suppressed in both GR-1(10 day-treatment group) and GR-2(4 day-treatment group) goats compared to saline controls but mean PRL concentrations remained lower in PG $F_{2{\alpha}}$ treated animals during post-treatment period. 6. The mean number of small and medium follicles present when PG $F_{2{\alpha}}$ was administrated was similar in all does but the increase in number of large follicles($\geq$4mm) tended to be higher in PG $F_{2{\alpha}}$ treated group than controls. These results suggest that concentrations of PG $F_{2{\alpha}}$ and estradiol-17$\beta$ were positively correlared with follicular diameter. We conclude that PG $F_{2{\alpha}}$ treatment stimulates follicular development similarly in both GR-1 and GR-2 group.

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ON THE DIRECT PRODUCTS AND SUMS OF PRESHEAVES

  • PARK, WON-SUN
    • Honam Mathematical Journal
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    • v.1 no.1
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    • pp.21-25
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    • 1979
  • Abelian군(群)의 presheaf에 관한 직적(直積)과 직화(直和)를 Category 입장에서 정의(定義)하고 presheaf $F_{\lambda}\;({\lambda}{\epsilon}{\Lambda})$들의 두 직적(直積)(또는 直和)은 서로 동형적(同型的) 관계(關係)에 있으며, 특히 ${\phi}:X{\rightarrow}Y$가 homeomorphism이라 하고 ${\phi}_*F$를 X상(上)의 presheaf F의 direct image이라 하면 (1) $({\phi}_*F, \;{\phi}_*(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$$({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직적(直積)일 때 오직 그때 한하여 $(F,\;(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$$(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직적(直積)이다. (2) $({\phi}_*F,\;{\phi}_*(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$$({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직화(直和)일 때 오직 그때 한하여 $(F,\;(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$$(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직화(直和)이다. Let $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ be an indexed set of presheaves of abelian group on topological space X. We can define the cartesian product $$\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda}$$ of $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ by $$(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U)=\prod_{{\lambda}{\epsilon}{\Lambda}}(F_{\lambda}(U))$$ for U open in X $${\rho}_v^u:\;(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U){\rightarrow}(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(V)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}(_{\lambda}{\rho}_v^u(s_{\lambda}))_{{\lambda}{\epsilon}{\Lambda}})$$ for $V{\subseteq}U$ open in X where $_{\lambda}{\rho}^U_V$ is a restriction of $F_{\lambda}$, And we have natural presheaf morphisms ${\pi}_{\lambda}$ and ${\iota}_{\lambda}$ such that ${\pi}_{\lambda}(U):\;({\prod}_\;F_{\lambda})(U){\rightarrow}F_{\lambda}(U)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}s_{\lambda})$ ${\iota}_{\lambda}(U):\;F_{\lambda}(U){\rightarrow}({\prod}\;F_{\lambda})(U)(s_{\lambda}{\rightarrow}(o,o,{\cdots}\;{\cdots}o,s_{\lambda},o,{\cdots}\;{\cdots}o)$ for $(s_{\lambda}){\epsilon}{\prod}_{\lambda}\;F_{\lambda}(U)$ and $(s_{\lambda}){\epsilon}F_{\lambda}(U)$.

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Proline-Rich Acidic Protein 1 (PRAP1) is a Target of ARID1A and PGR in the Murine Uterus

  • Kim, Tae Hoon;Jeong, Jae-Wook
    • Development and Reproduction
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    • v.23 no.3
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    • pp.277-284
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    • 2019
  • ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout ($Pgr^{cre/+}Arid1a^{f/f}$) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of $Pgr^{cre/+}Arid1a^{f/f}$ mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RT-qPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the $Pgr^{cre/+}Arid1a^{f/f}$ mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, $Pgr^{cre/+}Arid1a^{f/f}$, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the $Pgr^{cre/+}Arid1a^{f/f}$ and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.

An Analysis of the 1/f Noise Characteristics of Pocket Implanted MOSFETS (포켓 이온 주입된 MOSFET소자의 1/f 잡음 특성)

  • 이병헌;이기영
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.3
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    • pp.1-8
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    • 2004
  • The anomalous behavior of the 1/f noise of halo or pocket ion implanted MOSFETs is investigated. The model for the anomalous 1/f noise behaviors of MOSFETs, which consist of inhomogeneous conductance along the channel is improved within a regional approximation as previous works and presented in a fen directly applicable to halo MOSFETs. The presented model reduces to the previous results, discussed in the linear region operation, for small drain bias. Comparisons with experimental results show that the 1/f model based on the regional approach can be applicable for limited ranges, especially for sufficiently large gate bias voltages.

The Hereditary Phenomenon of Markings on the Dorsal Surface of Silkworm Eggs (II) (잠란상의 반문의 유전에 대하여 (II))

  • 박광의
    • Journal of Sericultural and Entomological Science
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    • v.3
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    • pp.29-31
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    • 1963
  • This work was carried out to know the hereditary phenomenon of. the egg markings with 4 races preserved at Sericultural Experiment Station from 1962 to 1963. The results are as follows: 1. When crosses (Fig. 1) were made between females showing reticulate egg marks and males showing collected egg markings, the F$_1$ markings were all reticulate. From the reciprocal cross was made between the collected marking females and the reticulate marking males (Fig. 2), all the F$_1$ progeny showed the collected egg markings. 2. In the F$_2$ the expected phenotype for reticulate markings (recessive) was not expressed but the collected markings (dominance) were always appeared. 3. When each F$_2$ moth was inbred, the usual 3:1 ratio was obtained. 4. The F$_1$ egg markings as well as the spindle shape egg were not determined by its own genes but by the genes of its mother, because those were formed before fertilization. The results of such influences, when they can be identified, are called material effects. And such a phenomenon was called pseudomaternal inheritance by Tanaka. (1919)

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THE LATTICE DISTRIBUTIONS INDUCED BY THE SUM OF I.I.D. UNIFORM (0, 1) RANDOM VARIABLES

  • PARK, C.J.;CHUNG, H.Y.
    • Journal of the Korean Mathematical Society
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    • v.15 no.1
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    • pp.59-61
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    • 1978
  • Let $X_1$, $X_2$, ${\cdots}$, $X_n$ be i.i.d. uniform (0,1) random variables. Let $f_n(x)$ denote the probability density function (p.d.f.) of $T_n={\sum}^n_{i=1}X_i$. Consider a set S(x ; ${\delta}$) of lattice points defined by S(x ; ${\delta}$) = $x{\mid}x={\delta}+j$, j=0, 1, ${\cdots}$, n-1, $0{\leq}{\delta}{\leq}1$} The lattice distribution induced by the p.d.f. of $T_n$ is defined as follow: (1) $f_n^{(\delta)}(x)=\{f_n(x)\;if\;x{\in}S(x;{\delta})\\0\;otherwise.$. In this paper we show that $f_n{^{(\delta)}}(x)$ is a probability function thus we obtain a family of lattice distributions {$f_n{^{(\delta)}}(x)$ : $0{\leq}{\delta}{\leq}1$}, that the mean and variance of the lattice distributions are independent of ${\delta}$.

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VALUE DISTRIBUTION OF SOME q-DIFFERENCE POLYNOMIALS

  • Xu, Na;Zhong, Chun-Ping
    • Bulletin of the Korean Mathematical Society
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    • v.53 no.1
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    • pp.29-38
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    • 2016
  • For a transcendental entire function f(z) with zero order, the purpose of this article is to study the value distributions of q-difference polynomial $f(qz)-a(f(z))^n$ and $f(q_1z)f(q_2z){\cdots}f(q_mz)-a(f(z))^n$. The property of entire solution of a certain q-difference equation is also considered.