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Genetic Analysis of the Life Cycle in Interspecific Hybrids of Pleurotus ostreatus and Pleurotus florida Following Protoplast Fusion (원형질 체융합에 의한 느타리버섯과 사철느타리버섯 체세포 잡종의 유전 분석)

  • Yoo, Young-Bok;You, Chang-Hyun;Park, Yong-Hwan;Peberdy, John F.
    • The Korean Journal of Mycology
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    • v.14 no.1
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    • pp.9-15
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    • 1986
  • Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.

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Cellulose Degradation and Extracellulat Enzymatic Activity of the Mud Flat in Sunchon Bay (순천만 갯벌 토양의 섬유소 분해능 및 체외효소 활성)

  • 백근식;최지혁;성치남
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.130-135
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    • 2000
  • Decomposition rate of organic matiter in the mud flat of Sunchon Bay was estimated. Physicochemical parameters, cellulose degradation rate. distribution of heterotrophic bacteria, and extracellular enzymatic activities were measured from August 1997 to July 1998. Soil temperatures, water contents, concentration of $PO_4$-P and organic matter were -1-~$30^{\circ}C$, 42.1-53.1%, 0.0779-0.1961 mgig and 1.99-7.64%, respectively. Decomposition rate of cellulose film ranged from 7.7 to 100%imonth, high in summer and low in winter. The number of heterotrophic bacteria ranged from $0.87{\times}10^6 to 3.6{\times}10^7 $CUFsIg dq soil. Enzymatic activities of phosphatase, $\alpha$-D-gluEosidase, $\beta$-D-glucosidase and cellobiohydrolase, which were measured as decomposition rate of methylumbelliferyl(MLiF)-substrate, were 152.23-1779.80 nMIhr, 2.67-202.18 nM/hr, 5.03-258.26 M h r and 3.42-63.07 nM/hr, respectively Cellulose degradaaon rate and extracellular extracellular enzymatic activities were conelated with each other, and showed high correlation coefticiency with soil temperature.

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CaM-5, a soybean calmodulin, is required for disease resistance against both a bacterial and fungal pathogen in tomato, Lycopersicum esculentum (대두 calmoduine유전자 SCaM-5를 발현하는 형질전환 토마토의 병 저항성 검정)

  • Lee, Hyo-Jung;Baek, Dong-Won;Lee, Ok-Sun;Lee, Ji-Young;Kim, Dong-Giun;Chung, Woo-Sik;Yun, Jae-Gil;Lee, Sin-Woo;Kwak, Sang-Soo;Nam, Jae-Seung;Kim, Doh-Hoon;Yun, Dae-Jin
    • Journal of Plant Biotechnology
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    • v.33 no.2
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    • pp.93-97
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    • 2006
  • The calmodulin as a Ca$^{2+}$-binding protein mediates cellular Ca$^{2+}$ signals in response to a wide array of stimuli in higher eukaryotes. Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca$^{2+}$ signals. SCaM-5 is a soybean calmodulin that is involved in plant defense signaling. Here, we constructed a SCaM-5 CDNA under control of CaMV 35S promoter and transformed it into tomato (Lycopersicon esculentum). The constitutive over-expression of SCaM-5 in tomato plants exhibited a high levels of pathogenesis-related (PR) gene expression, and conferred an enhanced resistance to two fungal pathogen (Phytophthora capsici, Fusarium oxysporum), and a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, this results collectively suggest that SCaM-5 plays an important role in plant defense of tomato.

Sense of Coherence and Coping Resources of Working Nurses at Hospital (병원근무 간호사의 통합성과 대처자원)

  • Park, Chai-Soon;Suh, Im-Sun;Oh, Jeong-Ah;Choi, Euy-Soon
    • Women's Health Nursing
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    • v.7 no.3
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    • pp.348-358
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    • 2001
  • This study was designed to identify the relationship between the level of sense of coherence(SOC) and coping resources of nurses working in hospital. The subjects of this study were 248 nurses working at C University hospital in Seoul. The data was collected during the period from July 1. to July 14. 2001. The insruments for this study were SOC scale by Antonovsky(1987) and Coping Resources Inventory by author. The analysis of data was done by simple statistics, ANOVA and Pearson correlation coefficient with SAS program. The results of this study were as follows: 1. Total mean SOC was 132.1/203.0 with a range 96.0 to 171.0. The mean score of this item was arranged according to three areas. The meaningfulness was 4.9, managability was 4.7, and comprehensibility was 4.2. 2. Total mean coping resources was 144.5/225.0 with a range 93.0 to 190.0. The highest mean score of item according to the area was social support 4.0 and the lowest mean score was self care coping 2.7. 3. A significant statistical difference between age, religion, spending money and SOC was found. 4. Significant statistical difference between age, educational level, spending money, working period and coping resources were found. 5. Coping resources by the level of SOC was high in the highest group of SOC(F=6.19, p=0.00). With respect to the area of coping resources, higher group of SOC was using much coping resources generally except area of leisure. 6. SOC was positively related to age(r=0.17), working periods(r=0.16), each SOC area, coping resources and all area of them except leisure. 7. The coping resources were positively related to age(r=0.21), working periods(r=0.21), SOC (r=0.49), each coping resources area, and each SOC area. In conclusion, we confirmed that the level of SOC of the subjects was low relatively and the high level of sense of coherence was related to the high score of coping resources. Therefore, it could also contribute to select suitable nurses in their fields. In the future, it's necessary to develop strategies improving SOC.

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Factors Influencing Posttraumatic Growth of North Korean Defectors in South Korea (북한이탈주민의 외상 후 성장에 영향을 미치는 요인)

  • Kim, Yun Ah;Kim, MiYoung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.5
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    • pp.332-338
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    • 2016
  • This study examined the posttraumatic growth of North Korean defectors, the relation between their characteristics and posttraumatic growth and the factors influencing their posttraumatic growth, and explored methods of improving their mental health status. 145 North Korean defectors, who were trained in B city and G city, participated in the survey from February 1 to March 10, 2016. The posttraumatic growth, resilience, self-esteem, deliberate rumination, and impact of the (traumatic) event were measured using the PTGI (Posttraumatic Growth Inventory), RS (Rumination Scale, Resilience Scale), SES (Self-esteem Scale) and ISR (Impact of Event). The data were analyzed using the t-test, ANOVA, Pearson correlation coefficient and stepwise multiple regression. The mean scores were 29.64 for posttraumatic growth. There were significant positive correlations between posttraumatic growth and resilience and between posttraumatic growth and rumination. The influence of the independent variables on the total posttraumatic growth was examined using regression analysis. Models including the variables (resilience, positive self-esteem, and rumination) explained 54.2% of the variance for the posttraumatic growth. These findings demonstrate that it is essential for nurses to continuously intervene and help North Korean defectors so as to promote their posttraumatic growth and resilience. Furthermore, it is also necessary for nurses to find ways to develop ideal interventions in order to activate deliberate rumination.

CMOS 소자 응용을 위한 Plasma doping과 Silicide 형성

  • Choe, Jang-Hun;Do, Seung-U;Seo, Yeong-Ho;Lee, Yong-Hyeon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.456-456
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    • 2010
  • CMOS 소자가 서브마이크론($0.1\;{\mu}m$) 이하로 스케일다운 되면서 단채널 효과(short channel effect), 게이트 산화막(gate oxide)의 누설전류(leakage current)의 증가와 높은 직렬저항(series resistance) 등의 문제가 발생한다. CMOS 소자의 구동전류(drive current)를 높이고, 단채널 효과를 줄이기 위한 가장 효율적인 방법은 소스 및 드레인의 얕은 접합(shallow junction) 형성과 직렬 저항을 줄이는 것이다. 플라즈마 도핑 방법은 플라즈마 밀도 컨트롤, 주입 바이어스 전압 조절 등을 통해 저 에너지 이온주입법보다 기판 손상 및 표면 결함의 생성을 억제하면서 고농도로 얕은 접합을 형성할 수 있다. 그리고 얕은 접합을 형성하기 위해 주입된 불순물의 활성화와 확산을 위해 후속 열처리 공정은 높은 온도에서 짧은 시간 열처리하여 불순물 물질의 활성화를 높여주면서 열처리로 인한 접합 깊이를 얕게 해야 한다. 그러나 접합의 깊이가 줄어듦에 따라서 소스 및 드레인의 표면 저항(sheet resistance)과 접촉저항(contact resistance)이 급격하게 증가하는 문제점이 있다. 이러한 표면저항과 접촉저항을 줄이기 위한 방안으로 실리사이드 박막(silicide thin film)을 형성하는 방법이 사용되고 있다. 본 논문에서는 (100) p-type 웨이퍼 He(90 %) 가스로 희석된 $PH_3$(10 %) 가스를 사용하여 플라즈마 도핑을 실시하였다. 10 mTorr의 압력에서 200 W RF 파워를 인가하여 플라즈마를 생성하였고 도핑은 바이어스 전압 -1 kV에서 60 초 동안 실시하였다. 얕은 접합을 형성하기 위한 불순물의 활성화는 ArF(193 nm) excimer laser를 통해 $460\;mJ/cm^2$의 에니지로 열처리를 실시하였다. 그리고 낮은 접촉비저항과 표면저항을 얻기 위해 metal sputter를 통해 TiN/Ti를 $800/400\;{\AA}$ 증착하고 metal RTP를 사용하여 실리사이드 형성 온도를 $650{\sim}800^{\circ}C$까지 60 초 동안 열처리를 실시하여 $TiSi_2$ 박막을 형성하였다. 그리고 $TiSi_2$의 두께를 측정하기 위해 TEM(Transmission Electron Microscopy)을 측정하였다. 화학적 결합상태를 분석하기 위해 XPS(X-ray photoelectronic)와 XRD(X-ray diffraction)를 측정하였다. 접촉비저항, 접촉저항과 표면저항을 분석하기 위해 TLM(Transfer Length Method) 패턴을 제작하여 I-V 특성을 측정하였다. TEM 측정결과 $TiSi_2$의 두께는 약 $580{\AA}$ 정도이고 morphology는 안정적이고 실리사이드 집괴 현상은 발견되지 않았다. XPS와 XRD 분석결과 실리사이드 형성 온도가 $700^{\circ}C$에서 C54 형태의 $TiSi_2$ 박막이 형성되었고 가장 낮은 접촉비저항과 접촉저항 값을 가진다.

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Weed Occurrence in Organic Apple Orchards by Different Cover Crops (유기재배 사과과원의 피복작물별 잡초발생)

  • Oh, Young-Ju;Song, Yang-Ik;Kang, Seok-Beom;Hong, Sun-Hee;Choi, Jin-Ho;Paik, Weon-Ki
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.24-30
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    • 2012
  • This study was conducted to select the cover crops in organic apple orchard by estimating the coverage of each cover crops and time-periodic weed occurrence. Seeding of Festuca myuros and Trifolium repens showed high contents of soil organic matter and phosphorus as compared with control. Seeding of Trifolium sp., Hordeum vulgare and Secale cereal reduced relatively occurrence of weed in May and S. cereal among others showed the greatest effect in June. In July, seeding F. arundinaceae and Poa pratensis showed low coverage and a lot of weeds occurred in their field. Fifty one species were occurred as weeds and it needs to removal of high and broadleaf weeds such as Chenopodium ficifolium, C. album var. centrorubru and Humulus japonicus in spring to increase the rate of ground cover. P. pratensis having the lowest cover rate showed the similar dry weight of weeds as compared with control. Trifolium sp. and Persicaria hydropiper had a high correlation with contents of soil organic matter and C. album var. centrorubru had a correlation with contents of soil phosphorus by CCA analysis.

Infection status with the metacercariae of heterophyid flukes in the brackishwater fish from Haenam-gun, Chollanam-do, Korea (전남 해남군산 반염수어의 이형흡충류 피낭유충 감염상)

  • 손운목;한경근
    • Parasites, Hosts and Diseases
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    • v.32 no.3
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    • pp.163-170
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    • 1994
  • An epidemiological survey was performed to know the infection status of heterophyid fluke metacercariae in the brackish water fishes purchased from a local market in Haenam-gun, Chollanam-do, Korea from November to December, 1991. A total 20 out of 30 shads (Konosiw punctatus) examined were infected with the metacercariae of H. continua (average number of larvae per an infected fish: 2.71). Of 20 perches (Lateolabroxjoponicurs), 19 were Infected with the metacercariae of H. continua (average number per an infected fish: 17.21. Of 30 mullets (Mugil cepholus) examined, 11 were Infected with the metacercariae of H. nocens (average number per an Infected fish: 51.5), 10 mullets harboured 31 and 349 metacercariae of f summa and 5. lucatum respectively. Of 30 gobies (Acnnthogobin jlcvimonus) examined, 3 were infEcted with the metacercariae of H. nucens (average number per an Infected fish: 32.0), 8 with those of H. continua (average number per an infected flesh: 12.6) and 3 with those of 5. JuscntuwL From the above results, It has been confirmed that the brackish water Ash from Haenam-gun, Chollanam-do are infected with numerous metacercarlae of H. nocens, H. continua, P. summo and S. fuscatum.

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Cohort profile: National Investigation of Birth Cohort in Korea study 2008 (NICKs-2008)

  • Kim, Ju Hee;Lee, Jung Eun;Shim, So Min;Ha, Eun Kyo;Yon, Dong Keon;Kim, Ok Hyang;Baek, Ji Hyeon;Koh, Hyun Yong;Chae, Kyu Young;Lee, Seung Won;Han, Man Yong
    • Clinical and Experimental Pediatrics
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    • v.64 no.9
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    • pp.480-488
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    • 2021
  • Background: An adequate large-scale pediatric cohort based on nationwide administrative data is lacking in Korea. Purpose: This study established the National Investigation of Birth Cohort in Korea study 2008 (NICKs-2008) based on data from a nationwide population-based health screening program and data on healthcare utilization for children. Methods: The NICKs-2008 study consisted of the Korean National Health Insurance System (NHIS) and the National Health Screening Program for Infants and Children (NHSPIC) databases comprising children born in 2008 (n=469,248) and 2009 (n=448,459) in the Republic of Korea. The NHIS database contains data on age, sex, residential area, income, healthcare utilization (International Classification of Diseases10 codes, procedure codes, and drug classification codes), and healthcare providers. The NHSPIC consists of 7 screening rounds. These screening sessions comprised physical examination, developmental screening (rounds 2-7), a general health questionnaire, and age-specific anticipatory guidance. Results: During the 10-year follow-up, 2,718 children (0.3%) died, including more boys than girls (hazard ratio, 1.145; P<0.001). A total of 848,048 children participated in at least 1 of the 7 rounds of the NHSPIC, while 96,046 participated in all 7 screening programs. A total of 823 infants (0.1%) weighed less than 1,000 g, 3,177 (0.4%) weighed 1,000-1,499 g, 37,166 (4.4%) weighed 1,500-2,499 g, 773,081 (91.4%) weighed 2,500-4,000 g, and 32,016 (5.1%) weighed over 4,000 g. There were 23,404 premature babies (5.5%) in 2008 compared to 23,368 (5.6%) in 2009. The developmental screening test indicated appropriate development in 95%-98% of children, follow-up requirements for 1%-4% of children, and recommendations for further evaluation for 1% of children. Conclusion: The NICKs-2008, which integrates data from the NHIS and NHSPIC databases, can be used to analyze disease onset prior to hospitalization based on information such as lifestyle, eating habits, and risk factors.

Sensory characteristics and preferences of rice-based distilled soju aged in different types of containers using Check-All-That-Apply (CATA) (숙성 기간과 저장용기를 달리한 쌀 증류식 소주의 Check-All-That-Apply (CATA)를 활용한 감각특성 및 기호도 분석)

  • Kim, Wan-Keun;Lee, Seung-Joo
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.362-368
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    • 2022
  • The sensory characteristics of nine rice-based distilled soju were determined using check-all-that-apply (CATA) profiling. A total of 53 consumers evaluated the soju for two appearance attributes, nine aroma attributes, nine flavor/taste attributes, four mouth-feel related sensory attributes, and overall desirability. The total sum of CATA terms indicated that 14 characteristics showed frequency differences of over 10 and that there were significant differences among nine samples for eleven sensory attributes as determined using Cochran's q test (p<0.05). Based on correspondence analysis of CATA data, the samples were primarily separated by the first dimension, which accounted for 89% of the total variance among samples. The "brown color," "fruit taste," and "grain aroma" characteristics had higher frequencies than those for the "white color," "acetone aroma," and 'alcohol taste" characteristics. Overall, there was a higher preference for oak-aged samples than for samples aged in other containers. "sweet aroma', 'fruit aroma," and "sweet taste" seemed to positively affect consumer preferences, while "bitter taste," "alcohol taste," and "acetone aroma" appeared to negatively affect consumer preferences as determined by principal coordinate analysis.