Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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CaM-5, a soybean calmodulin, is required for disease resistance against both a bacterial and fungal pathogen in tomato, Lycopersicum esculentum

대두 calmoduine유전자 SCaM-5를 발현하는 형질전환 토마토의 병 저항성 검정

  • Lee, Hyo-Jung (Division of Applied Life Science, Gyeongsang National University) ;
  • Baek, Dong-Won (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Ok-Sun (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Ji-Young (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Dong-Giun (Division of Applied Life Science, Gyeongsang National University) ;
  • Chung, Woo-Sik (Division of Applied Life Science, Gyeongsang National University) ;
  • Yun, Jae-Gil (College of Life Science and Natural Resources, Jinju National University) ;
  • Lee, Sin-Woo (College of Life Science and Natural Resources, Jinju National University) ;
  • Kwak, Sang-Soo (Environmental Biotechnology, Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Nam, Jae-Seung (Faculty of Molecular Biotechnology, Dong-A University) ;
  • Kim, Doh-Hoon (Faculty of Molecular Biotechnology, Dong-A University) ;
  • Yun, Dae-Jin (Division of Applied Life Science, Gyeongsang National University)
  • 이효정 (경상대학교 대학원 응용생명과학부) ;
  • 백동원 (경상대학교 대학원 응용생명과학부) ;
  • 이옥선 (경상대학교 대학원 응용생명과학부) ;
  • 이지영 (경상대학교 대학원 응용생명과학부) ;
  • 김동균 (경상대학교 대학원 응용생명과학부) ;
  • 정우식 (경상대학교 대학원 응용생명과학부) ;
  • 윤재길 (진주산업대학교 생명자원과학대학 작물생명과학과) ;
  • 이신우 (진주산업대학교 생명자원과학대학 작물생명과학과) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구센터) ;
  • 남재성 (동아대학교 생명자원과학대학 분자생명공학부) ;
  • 김도훈 (동아대학교 생명자원과학대학 분자생명공학부) ;
  • 윤대진 (경상대학교 대학원 응용생명과학부)
  • Published : 2006.06.30
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Abstract

The calmodulin as a Ca$^{2+}$-binding protein mediates cellular Ca$^{2+}$ signals in response to a wide array of stimuli in higher eukaryotes. Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca$^{2+}$ signals. SCaM-5 is a soybean calmodulin that is involved in plant defense signaling. Here, we constructed a SCaM-5 CDNA under control of CaMV 35S promoter and transformed it into tomato (Lycopersicon esculentum). The constitutive over-expression of SCaM-5 in tomato plants exhibited a high levels of pathogenesis-related (PR) gene expression, and conferred an enhanced resistance to two fungal pathogen (Phytophthora capsici, Fusarium oxysporum), and a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, this results collectively suggest that SCaM-5 plays an important role in plant defense of tomato.

농작물 생산에 있어서 병원균 침입에 의한 피해를 줄이는 것은 아주 중요한 과제이다. 식물은 스스로도 생체 방어 신호 전달 기작을 가지고 있지만 그 피해를 줄이는데 있어서 한계가 있다. 본 연구는 콩과 식물에서 분리한 식물생체방어 신호전달 유전자인 SCaM-5를 토마토에 형질전환하여 형질전환 식물체를 작성하고 병 저항성에 관한 실험을 수행한 것이다. SCaM-5 유전자가 형질전환 된 토마토에서는 pathogen-related(PR-5) 유전자를 항상 발현시킴으로서 계속적으로 식물 생체방어 신호기작을 활성 시킨다는 사실을 확인하였다. 또한, SCaM-5를 형질전환 시킨 토마토는 식물에 막대한 피해를 주는 중요한 곰팡이 (P. capsici와 F. oxysporm)와 bacteria (Pst DC3000)에 대하여 병 저항성을 가진다는 것을 검증하였다.

Keywords

References

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