• KSBB Journal
• /
• v.22 no.5
• /
• pp.345-350
• /
• 2007

Phosphosugars are found in all living organisms and are commercially valuable compounds with possible applications in the development of a wide range of specialty chemicals and medicines. In carbohydrate metabolism, fructose 6-phosphate (F6P) is an essential intermediate formed by phosphorylation of 6' position of fructose in glycolysis, gluconeogenesis, pentose phosphate pathway and Calvin cycle. In glycolysis, F6P lies within the glycolysis metabolic pathway and is produced by isomerisation of glucose 6-phosphate. For large-scale production, F6P could be produced from starch using many enzymes such as pullulanase, starch phosphorylase, isomerase and mutase. In enzymatic reactions carried out at high temperatures, the solubility of starch is increased and microbial contamination is minimized. Thus, thermophile-derived enzymes are preferred over mesophile-derived enzymes for industrial applications using starch. Recently, we reported the production of glucose 1-phosphate (G1P) from starch by Thermus caldophilus GK24 enzymes. Here we report the production of F6P from starch through three steps; from starch to glucose 1-phosphate (glucan phosphorylase, GP), then glucose 6-phosphate (phosphoglucomutase, GM) and then F6P (phosphoglucoisomerase, GI). Using 200 L of 1.2% soluble starch solution in potassium phosphate buffer, 1,253 g of G1P were produced. Then, 30% yields of F6P were attained at the optimum reaction conditions of GM : G1 (1 : 2.3), 63.5$^{\circ}C$, and pH 6.85. The optimum conditions were found by response surface methodology and the theoretical values were confirmed by the experiments. The optimum starch concentrations were 20 g/L under the given conditions.

• Journal of Life Science
• /
• v.29 no.3
• /
• pp.376-381
• /
• 2019

To construct useful yeast strain for bioethanol production, we improved yeast harboring various phenotypes by using yeast protoplast fusion method. In this study, S. cerevisiae BYK-F11 strain which have ethanol tolerance, thermotolerance and ${\beta}-glucanase$ activity and P. $stipitis{\Delta}ura$ strain which has xylose metabolism pathway were fused by genome shuffling. P. $stipitis{\Delta}ura$ strain was constructed for protoplast fusion by URA3 gene disruption, resulting in uracil auxotroph. By protoplast fusion, several fused cells were selected and BYKPS-F8 strain (fused cell) showing both karyotypes from two parent strains (S. cerevisiae BYK-F11 and P. $stipitis{\Delta}ura$ strain) among 22 fused cells was finally selected. Sequentially, various phenotypes such as ${\beta}-glucanase$ activity, xylose utility, ethanol tolerance, thermotolerance and ethanol productivity were analyzed. The BYKPS-F8 strain obtained ${\beta}-glucanase$ activity from BYK-F11 strain and 1.2 fold increased xylose utility from P. $stipitis{\Delta}ura$ strain. Also, the BYKPS-F8 strain showed thermotolerance at $40^{\circ}C$ and increased ethanol tolerance in medium containing 8% ethanol. In this fused cell, 7.5 g/l ethanol from 20 g/l xylose was produced and the multiple phenotypes were stably remained during long term cultivation (260 hr). It was proved that novel biological system (yeast strains) is easily and efficiently bred by protoplast fusion among yeasts having different genus.

• 한국전산유체공학회:학술대회논문집
• /
• 2008.08a
• /
• pp.53.4-53.4
• /
• 2008

• Natural Product Sciences
• /
• v.13 no.3
• /
• pp.251-254
• /
• 2007

Dioxopiperazine alkaloids, 12R,13S-dihydroxyfumitremorgin C (1), fumitremorgin C (2), and brevianamide F (3), were isolated from the marine-derived fungus Pseudallescheria, and the absolute stereostructures of compounds 1 - 3 were elucidated on the basis of chemical and physicochemical evidence. Compounds 1 - 3 showed an antibacterial activity against Staphylococcus aureus, methicillin-resistant S. aureus, and multidrug-resistant S. aureus. The MIC (minimum inhibitory concentration) values of compounds 1 - 3 were 125 ${\mu}g/mL$ for all strains.

• Journal of the Korean Mathematical Society
• /
• v.39 no.5
• /
• pp.783-800
• /
• 2002

Let B denote the unit ball in $C^n$, and ν the normalized Lebesgue measure on B. For $\alpha$ > -1, define $dv_\alpha$(z) ＝ $c_\alpha$$(1-\midz\mid^2)^{\alpha}$dν(z), z $\in$ B. Here $c_\alpha$ is a positive constant such that $v_\alpha$(B) ＝ 1. Let H(B) denote the space of all holomorphic functions in B. For $p\geq1$, define the Bergman-Privalov space $(AN)^{p}(v_\alpha)$ by $(AN)^{p}(v_\alpha)$ = ${f\inH(B)$ : $\int_B{log(1+\midf\mid)}^pdv_\alpha\;<\;\infty}$ In this paper we prove that a function $f\inH(B)$ is in $(AN)^{p}$$(v_\alpha)$ if and only if $(1+\midf\mid)^{-2}{log(1+\midf\mid)}^{p-2}\mid\nablaf\mid^2\;\epsilon\;L^1(v_\alpha)$ in the case 1＜p＜$\infty$, or $(1+\midf\mid)^{-2}\midf\mid^{-1}\mid{\nabla}f\mid^2\;\epsilon\;L^1(v_\alpha)$ in the case p ＝ 1, where $nabla$f is the gradient of f with respect to the Bergman metric on B. This is an analogous result to the characterization of the Hardy spaces by M. Stoll [18] and that of the Bergman spaces by C. Ouyang-W. Yang-R. Zhao [13].

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.10
• /
• pp.1445-1450
• /
• 2005

This experiment was conducted to evaluate the effect of feed withdrawal (F) and heat acclimatization (A) on malebroiler and -layer chickens responses to acute heat stress (AHS) at four weeks of age. Totals of ninety male chicks of broiler or layer type were randomly allocated into 30 pens of grower batteries with raised wire floors. Chicks were subjected to F and A three times a week through the first three weeks of age. At each time, feed withdrawal and heat acclimatization (T = $35^{\circ}C$) lasted for six and four hours, respectively. Feed consumption (FC), body weight (BW), and feed conversion ratio (FCR) were recorded weekly for broiler type chickens only. At four weeks of age, all groups of chickens were exposed to AHS (T = $39{\pm}1^{\circ}C$) for three hours. Before and after AHS challenge, body temperature (Tb), heterophil (H), and lymphocyte (L) counts were recorded, and H/L ratio was calculated. Antibody (Ab) response to sheep red blood cells (SRBC) was assessed from all treatments without being exposed to AHS. Group F of broiler-type chickens weighed less (p<0.05) compared to control group. Also, both A and F groups of broiler-type chickens consumed less (p<0.05) feed when compared to control group. Acute heat stress elevated Tb of all treatment groups, however the increase was more profound (p<0.001) in broiler chicks. Broiler chicks of both A and F groups showed a tendency to have higher (p = 0.08) Tb when compared to control group. Acute heat stress elevated (p<0.001) H/L ratio in both types of chickens. Broiler chicks maintained higher (p<0.001) H/L ratio. Both F and A groups reduced (p<0.01) the level of elevation in H/L ratio compared to control groups of both types of chickens. Neither A nor F group affected the Ab production in response to SRBC. However, there was a tendency towards higher Ab responses in F group when compared to other groups in both types of chickens. Results of the present study demonstrate that previous history of feed withdrawal or episodes of heat exposures improved chicks'physiological withstanding of AHS and a tendency to improved humoral immune response.

• Korean Journal of Food Science and Technology
• /
• v.47 no.3
• /
• pp.293-298
• /
• 2015

This study aimed at characterizing the rheological properties and molecular weight of tara gum fractionated with ammonium sulfate. Tara gum was separated into six fractions (F1-F6) at different concentrations of ammonium sulfate, ranging from 12.21 to 28.67% (w/w). The yield of the tara gum fractions ranged between 4.98 and 17.47%, and their intrinsic viscosity ranged from 9.38 to 12.44 dL/g. The highest values of Huggins coefficient (k') and viscosity-molecular mass were observed in fraction F3. The shear viscosity of the tara gum fractions was measured by a cone-plate viscometer, clearly showing shear thinning behavior. Size-exclusion chromatography results showed that the molecular weight ranged between 635.42 and 776.71 kg/mol, and the F3 fraction exhibited higher values of molecular weight.

• Honam Mathematical Journal
• /
• v.34 no.3
• /
• pp.409-422
• /
• 2012

Let $\mathcal{L}$ be a subspace lattice on a Hilbert space $\mathcal{H}$. And let X and Y be operators acting on a Hilbert space $\mathcal{H}$. Let $sp(x)=\{{\alpha}x\;:\;{\alpha}{\in}\mathcal{C}\}$ $x{\in}\mathcal{H}$. Assume that $\mathcal{H}=\overline{range\;X}{\oplus}sp(h)$ for some $h{\in}\mathcal{H}$ and < $h$, $E^{\bot}Xf$ >= 0 for each $f{\in}\mathcal{H}$ and $E{\in}\mathcal{L}$. Then there exists an operator A in Alg$\mathcal{L}$ such that AX = Y if and only if $sup\{\frac{{\parallel}E^{\bot}Yf{\parallel}}{{\parallel}E^{\bot}Yf{\parallel}}\;:\;f{\in}H,\;E{\in}\mathcal{L}\}$ = K < ${\infty}$. Moreover, if the necessary condition holds, then we may choose an operator A such that AX = Y and ${\parallel}||A{\parallel}=K$.

• Journal of the Korean Mathematical Society
• /
• v.50 no.3
• /
• pp.557-578
• /
• 2013

For an ideal $\mathcal{I}$ of a preadditive category $\mathcal{A}$, we study when the canonical functor $\mathcal{C}:\mathcal{A}{\rightarrow}\mathcal{A}/\mathcal{I}$ is local. We prove that there exists a largest full subcategory $\mathcal{C}$ of $\mathcal{A}$, for which the canonical functor $\mathcal{C}:\mathcal{C}{\rightarrow}\mathcal{C}/\mathcal{I}$ is local. Under this condition, the functor $\mathcal{C}$, turns out to be a weak equivalence between $\mathcal{C}$, and $\mathcal{C}/\mathcal{I}$. If $\mathcal{A}$ is additive (with splitting idempotents), then $\mathcal{C}$ is additive (with splitting idempotents). The category $\mathcal{C}$ is ample in several cases, such as the case when $\mathcal{A}$=Mod-R and $\mathcal{I}$ is the ideal ${\Delta}$ of all morphisms with essential kernel. In this case, the category $\mathcal{C}$ contains, for instance, the full subcategory $\mathcal{F}$ of Mod-R whose objects are all the continuous modules. The advantage in passing from the category $\mathcal{F}$ to the category $\mathcal{F}/\mathcal{I}$ lies in the fact that, although the two categories $\mathcal{F}$ and $\mathcal{F}/\mathcal{I}$ are weakly equivalent, every endomorphism has a kernel and a cokernel in $\mathcal{F}/{\Delta}$, which is not true in $\mathcal{F}$. In the final section, we extend our theory from the case of one ideal$\mathcal{I}$ to the case of $n$ ideals $\mathcal{I}_$, ${\ldots}$, $\mathca{l}_n$.

• Journal of the Chungcheong Mathematical Society
• /
• v.27 no.4
• /
• pp.697-705
• /
• 2014

For a complex measure ${\mu}$ on B and $f{\in}L^2_a(B)$, the Toeplitz operator $T_{\mu}$ on $L^2_a(B,dv)$ with symbol ${\mu}$ is formally defined by $T_{\mu}(f)(w)=\int_{B}f(w)\bar{K(z,w)}d{\mu}(w)$. We will investigate properties of the Toeplitz operator $T_{\mu}$ with symbol ${\mu}$. We define the Toeplitz type operator $T^r_{\psi}$ with symbol ${\psi}$, $$T^r_{\psi}f(z)=c_r\int_{B}\frac{(1-{\parallel}w{\parallel}^2)^r}{(1-{\langle}z,w{\rangle})^{n+r+1}}{\psi}(w)f(w)d{\nu}(w)$$. We will also investigate properties of the Toeplitz type operator with symbol ${\psi}$.