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Functional Analysis of Bacillus subtilis Isolates and Biological Control of Red Pepper Powdery Mildew Using Bacillus subtilis R2-1 (Bacillus subtilis 균주의 기능성 분석 및 이를 활용한 고추 흰가루병 방제)

  • Kim, Yong-Ki;Hong, Sung-Jun;Shim, Chang-Ki;Kim, Min-Jeong;Choi, Eun-Jung;Lee, Min-Ho;Park, Jong-Ho;Han, Eun-Jung;An, Nan-Hee;Jee, Hyeong-Jin
    • Research in Plant Disease
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    • v.18 no.3
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    • pp.201-209
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    • 2012
  • The multi-function of 18 Bacillus subtilis isolates collected from agricultural extension centers of local government and National Academy of Agricultural Science was investigated by measuring their antifungal activities against five plant pathogens, such as Rhizoctonia solani, Colletotrichum acutatum, Fusarium oxysporum, Magnaporthe oryzae and Phytophthora capsici, phosphorus solubilization ability, production of indole acetic acid (IAA) and siderophore, and nitrogen fixation. The B. subtilis isolates showed antifungal activity against several plant pathogens and nitrogen fixation activity, and produced siderophore and IAA. They could control pepper powdery mildew (Leveillula taurica), but there was no difference in control efficacy among the B. subtilis isolates. In fields, the control efficacy of B. subtilis R2-1 ($10^8$ cells/ml) was compared with two microbial fungicides, Q-pect and Topsid. In 2009, the control efficacy of B. subtilis R2-1 (37.7%) was lower than that of Topsid (47.6%), but higher than that of Q-pect (25.7%). In 2010, the control efficacy of B. subtilis R2-1 (83.3%) was higher than that of Topsid (67.9%). In order to elucidate mode of action of B. subtilis R2-1 for controlling pepper powdery mildew, spore germination rates of pepper powdery mildew pathogen collected on treated leaves was investigated when suspensions of B. subtilis R2-1 and two microbial fungicides (Q-pect and Topsid) were foliar-sprayed. They highly suppressed spore germination of the pathogen with inhibition values of 84.2% for B. subtilis R2-1, 97.9% for Q-pect and 94.7% for Topsid. Further study on the mass-culturing method and formulation is needed for development of a microbial fungicide.

A Study on the Relationship of Learning, Innovation Capability and Innovation Outcome (학습, 혁신역량과 혁신성과 간의 관계에 관한 연구)

  • Kim, Kui-Won
    • Journal of Korea Technology Innovation Society
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    • v.17 no.2
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    • pp.380-420
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    • 2014
  • We increasingly see the importance of employees acquiring enough expert capability or innovation capability to prepare for ever growing uncertainties in their operation domains. However, despite the above circumstances, there have not been an enough number of researches on how operational input components for employees' innovation outcome, innovation activities such as acquisition, exercise and promotion effort of employee's innovation capability, and their resulting innovation outcome interact with each other. This trend is believed to have been resulted because most of the current researches on innovation focus on the units of country, industry and corporate entity levels but not on an individual corporation's innovation input components, innovation outcome and innovation activities themselves. Therefore, this study intends to avoid the currently prevalent study frames and views on innovation and focus more on the strategic policies required for the enhancement of an organization's innovation capabilities by quantitatively analyzing employees' innovation outcomes and their most suggested relevant innovation activities. The research model that this study deploys offers both linear and structural model on the trio of learning, innovation capability and innovation outcome, and then suggests the 4 relevant hypotheses which are quantitatively tested and analyzed as follows: Hypothesis 1] The different levels of innovation capability produce different innovation outcomes (accepted, p-value = 0.000<0.05). Hypothesis 2] The different amounts of learning time produce different innovation capabilities (rejected, p-value = 0.199, 0.220>0.05). Hypothesis 3] The different amounts of learning time produce different innovation outcomes. (accepted, p-value = 0.000<0.05). Hypothesis 4] the innovation capability acts as a significant parameter in the relationship of the amount of learning time and innovation outcome (structural modeling test). This structural model after the t-tests on Hypotheses 1 through 4 proves that irregular on-the-job training and e-learning directly affects the learning time factor while job experience level, employment period and capability level measurement also directly impacts on the innovation capability factor. Also this hypothesis gets further supported by the fact that the patent time absolutely and directly affects the innovation capability factor rather than the learning time factor. Through the 4 hypotheses, this study proposes as measures to maximize an organization's innovation outcome. firstly, frequent irregular on-the-job training that is based on an e-learning system, secondly, efficient innovation management of employment period, job skill levels, etc through active sponsorship and energization community of practice (CoP) as a form of irregular learning, and thirdly a model of Yί=f(e, i, s, t, w)+${\varepsilon}$ as an innovation outcome function that is soundly based on a smart system of capability level measurement. The innovation outcome function is what this study considers the most appropriate and important reference model.

Effect of Oral Health Education Practice and Self-efficacy of Teacher on Oral Health Attitude (교사의 구강보건교육 실천과 자기효능감이 구강보건교육 태도에 미치는 영향)

  • Cho, Young-Sik;Hwang, Yoo-Jin;Bae, Hyun-Sook;Kim, Seo yune
    • Journal of dental hygiene science
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    • v.9 no.1
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    • pp.99-108
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    • 2009
  • The purpose of this study was to investigate the effect of oral health education practice and schoolteacher's self-efficacy on oral health attitude. The data of 217 dental hygienists was subjected to path analysis using AMOS 16.0. Oral health knowledge, attitude, self-efficacy and oral health education practice of schoolteacher were measurement variables of three path models. The goodness of fitness of Knowledge-Attitude-Practice model and Knowledge-Attitude-Self efficacy -Practice model were acceptable. The regression weights of two models were statistically significant. Oral health education practice of schoolteacher gave impact on oral health attitude by mediation of self-efficacy of schoolteacher. Self-efficacy of schoolteacher was a important determinant of oral health education practice. Oral health attitude and practice were supposed to be mutual determinants of health behavior models.

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Continuity Simulation and Trend Analysis of Water Qualities in Incoming Flows to Lake Paldang by Log Linear Models (로그선형모델을 이용한 팔당호 유입지류 수질의 연속성 시뮬레이션과 경향 분석)

  • Na, Eun-Hye;Park, Seok-Soon
    • Korean Journal of Ecology and Environment
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    • v.36 no.3 s.104
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    • pp.336-343
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    • 2003
  • Two types of statistical models, simple and multivariate log linear models, were studied for continuity simulation and trend analysis of water qualities in incoming flows to Lake Paldang. Water quality is a function of one independent variable (flow) in the simple log linear model, and of three different variables (flow, time, and seasonal cycle) in multivariate model. The independent variables act as surrogate variables of water quality in both models. The model coefficients were determined by the monthly data. The water qualities included 5-day Biochemical Oxygen Demand ($BOD_5$), Total Nitrogen (TN), and Total Phosphorus (TP) measured from 1995 to 2000 in the South and the North branches of Han River and the Kyoungan Stream. The results indicated that the multivariate model provided better agreements with field measurements than the simple one in a31 attempted cases. Flow dependency, seasonality, and temporal trends of water quality were tested on the determined coefficients of the multivariate model. The test of flow dependency indicated that BOD concentrations decreased as the water flow increased. In TN and TP concentrations, however, there were no discernible flow effects. From the temporal trend analyses, the following results were obtained: 1) no trends on BOD at all three upstreams, 2) increase on TN at the South Branch and the Kyoungan Stream, 3)decrease on TN at the North Branch,4) no trends on TP at the North and the South Branches and 5) increase on TP at the Kyoungan Stream by 3 to 8% per years. The seasonality test showed that there were significant seasonal variations in all three water qualities at three incoming flows.

Comparison of Urine Iodine/Creatinine ratio between Patients following Stringent and Less Stringent Low Iodine Diet for Radioiodine Remnant Ablation of Thyroid Cancer (갑상선암의 방사성요오드치료를 위한 저요오드식이 방법 차이에 따른 소변 중 요오드/크레아티닌 비의 비교)

  • Roh, Jee-Ho;Kim, Byung-Il;Ha, Ji-Su;Chang, Sei-Joong;Shin, Hye-Young;Choi, Joon-Hyuk;Kim, Do-Min;Kim, Chong-Soon
    • Nuclear Medicine and Molecular Imaging
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    • v.40 no.6
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    • pp.322-326
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    • 2006
  • A low iodine diet (LID) for $1{\sim}2$ weeks is recommended for patients who undergoing radioiodine remnant ablation. However, the LID educations for patients are different among centers because there is no concrete recommendation for protocol of LID. In this investigation, we compared two representative types of LID protocols performed in several centers in Korea using urine iodine to creatinine ratio (urine I/Cr). Methods: From 2006, April to June, patients referred to our center for radioiodine remnant ablation of thyroid cancer from several local hospitals which had different LID protocols were included. We divided into two groups, stringent LID for 1week and less stringent LID for 2 weeks, then measured their urine I/Cr ratio with spot urine when patients were admitted to the hospital. Results: Total 27 patients were included in this investigation (M:F=1:26; 13 in one-week stringent LID; 14 in two-week less stringent LID. Average of urine I/Cr ratio was $127.87{\pm}78.52{\mu}g/g$ in stringent LID for 1 week, and $289.75{\pm}188.24{\mu}g/g$ in less stringent LID for 2 weeks. It was significantly lower in stringent LID for 1 week group (p=0.008). The number of patients whose urine I/Cr ratios were below $100{\mu}g/g$ was 6 of 13 in stringent LID for 1 week group, and 3 of 14 in less stringent LID for 2 weeks group. Conclusion: Stringent LID for 1 week resulted in better urinary I/Cr ratio in our investigation compared with the other protocol. However it still resulted in plenty of inadequate range of I/Cr ratio, so more stringent protocol such as stringent LID for 2 weeks is expected more desirable.

Tc-99m ECD Brain SPECT in Patients with Traumatic Brain Injury: Evaluating Distribution of Hypoperfusion and Assesment of Cognitive and Behavioral Impairment in Relation to Thalamic Hypoperfusion (뇌외상 환자의 Tc-99m ECD 뇌 SPECT에서 뇌 혈류감소의 분포 및 시상의 혈류감소에 대한 인지 및 행동장애 평가)

  • Park, Soon-Ah;Lim, Seok-Tae;Sohn, Myung-Hee
    • The Korean Journal of Nuclear Medicine
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    • v.34 no.6
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    • pp.445-455
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    • 2000
  • Purpose: We evaluated the distribution of hypoperfusion in patients with traumatic brain injury (TBI) and the relationship of thalamic hypoperfusion to severity of cognitive and behavioral sequelae. Materials and Methods: Tc-99m ECD SPECT and MRI were performed in 103 patients (M/F=81/22, mean age $34.7{\pm}15.4$ yrs) from 0.5 to 55 months (mean 10.3 months) after TBI. The patients were divided into three groups showing no abnormalities (G1), focal (G2) and diffuse injury (G3) on MRI. Psychometric tests assessed 11 cognitive or behavioral items. In all patients, we evaluated the distribution of hypoperfused areas in SPECT, and in 57/103 patients, neuropsychological (NP) abnormalities in patients with thalamic hypoperfusion were compared with those of patients without thalamic hypoperfusion. Results: The perfusion deficits were most frequently located in the frontal lobe (G1, 42.3%: G2 34.5%: G3 33.3%), temporal lobe ($24{\sim}26%$) thalami ($21{\sim}22.4%$), parietal and occipital lobe (${\leq}10%$). Numbers of NP abnormalities in the cases of cortical hypoperfusion with or without concomitant thalamic hypoperfusion were following: the former $4.7{\pm}1.5$ and the latter $3.2{\pm}1.4\;in\;G1,\;5.0{\pm}1.1\;and\;4.8{\pm}1.2\;in\;G2,\;6.8{\pm}1.8\;and\;6.3{\pm}1.1\;in\;G3$, respectively. This difference according to thalamic hypoperfusion was significant in G1 (p=0.002), but was not significant in G2 or G3. Conclusion: SPECT in patients with TBI had demonstrated hypoperfusion mostly involving the frontal, temporal and thalami. In normal group on MRI, frontal hypoperfusion was more prominent than that of any other group, Furthermore in this group, SPECT could predict severity of NP outcome by concomitant thalamic hypoperfusion with cerebral cortical abnormalities.

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The Effect of 131I Therapy by Taking in Laxatives (방사성요오드 치료 시 완하제 투여 효과)

  • Kil, Sang-Hyeong;LEE, Hyo-Yeong;Park, Kwang-Yeol;Jo, Kyung-Nam;Baek, Seung-Jo;Hwang, Kyo-Min;Cho, Seong-Mook;Choi, Jae-Hyeok
    • The Korean Journal of Nuclear Medicine Technology
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    • v.18 no.1
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    • pp.3-9
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    • 2014
  • Purpose: Our goals were to evaluate the effect of high dose radioiodine treatment for thyroid cancer by taking in laxatives. Materials and Methods: Twenty patients(M:F=13:7, age $46.3{\pm}8.1\;yrs$) who underwent high dose radioiodine treatment were seperated into Group 1 taking $^{131}I$ 5,500 MBq and Group 2 with the use of laxatives after taking $^{131}I$ 5,500 MBq. The whole body was scanned 16 hours and 40 hours after taking radioactive iodines by using gamma camera, the ROIs were drawn on the gastro-intestinal tract and thigh for calculation of reduction ratio. At particular time during hospitalization, the radioactivity remaining in the body was measured in 1 meter from patient by using survey meter (RadEye-G10, Thermo Fisher Scientific, USA). Schematic presentation of an Origin 8.5.1 software was used for spatial dose rate. Statistical comparison between groups were done using independent samples t-test. P value less than 0.05 was regarded as statistically significant. Results: The reduction ratio in gastro-intestinal 16 hours and 40 hours after taking laxatives is $42.1{\pm}6.3%$ in Group 1 and $72.1{\pm}6.4%$ in Group 2. The spatial dose rate measured when discharging from hospital was $23.8{\pm}6.7{\mu}Sv/h$ in Group 1 and $8.2{\pm}2.4{\mu}Sv/h$ in Group 2. The radioactivity remaining in the body is much decreased at the patient with laxatives(P<0.05). Conclusion: The use in combination with laxatives is helpful for decreasing radioactivity remaining in the body. The radioactive contamination could be decreased at marginal individuals from patients.

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Growth Characteristics and Productivity of Single Cross Maize New Hybrid for Silage and Grain, 'Yanganok' (사료용 옥수수 신품종 '양안옥'의 생육특성 및 수량성)

  • Son, Beom Young;Baek, Seong Bum;Kim, Jung Tae;Lee, Jin Seok;Ku, Ja Hwan;Kim, Sun Lim;Jung, Gun Ho;Kwon, Young Up;Ji, Hee Jung;Huh, Chang Suk;Park, Jong Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.94-99
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    • 2013
  • Yanganok, a new single cross variety, was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2012. This hybrid, which has a high yield of grain was produced by crossing two inbred lines, KS164 and KS163. KS164 is the seed parent and KS163 is the pollen parent of Yanganok. It is a yellow-orange intermediate maize hybrid (Zea mays L.). After a preliminary yield trial and advanced yield trial of Yanganok (Suwon180) in Suwon for 2 years, a regional yield trial was carried out for its growth characteristics, and yields were determined at 4 different locations from 2009 to 2012, with the exception of 2010. It was named 'Yanganok'. The silking date of Yanganok is 2 days earlier than that of the check hybrid, Kwangpyeongok. Plant height of Yanganok is similar to Kwangpyeongok, but its ear height ratio is higher than that of Kwangpyeongok. It has resistance to lodging. Stay-green features of Yanganok are similar to those of Kwangpyeongok. The ear ratio of Yanganok is similar to Kwangpyeongok. It has moderate resistance to southern leaf blight (Bipolaris maydis) and strong resistance to northern leaf blight (Exserohilum turcicum). It has moderate resistance to black streaked dwarf virus (BSDV), ear lot and corn borer. The dry matter yield of Yanganok, 17.45 ton/ha, was similar to that of Kwangpyeongok. The total digestible nutrients (TDN) yield of Yanganok, 11.96 ton/ha, was similar to that of Kwangpyeongok. The grain yield of Yanganok, 8.32 ton/ha was similar to that of Jangdaok. Seed production of Yanganok went well due to a good match during crossing between the seed parent, KS164, and the pollen parent, KS163, in Yeongwol, and F1 seed yield was 1.79 ton/ha. Yanganok would be a suitable cultivar to all plain areas in the Korean peninsula.

The Effects of Emotional Perception on Major Satisfaction among Students at the Department of Dental Hygiene (치위생과 학생의 정서적 인식이 전공만족도에 미치는 영향)

  • Yu, Ji-Su;Choi, Su-Young
    • Journal of dental hygiene science
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    • v.10 no.5
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    • pp.307-314
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    • 2010
  • This study aimed to measure such features of emotional responses perceived by students as learning climate, department living stress, and perceived helplessness to analyze their effects on major satisfaction among students at the department of dental hygiene; to do this, a survey was conducted with 431 students, regardless of college year, who were at the department of dental hygiene in four colleges in Gyeonggi Province, Daejeon, and Chungcheong Province. An existing emotion scale which went through the generalization process was used to draw a multiple model in the combination form in order to collect emotional factors affecting college students' satisfaction with their major, which had existed as a hypothetical proposition, and make overall interpretation of relevance through the explainable, predictable modeling process by measuring emotional factors and phenomenal description of the level of general perception. The results showed that major satisfaction was very significantly affected by emotional features among students at the department of dental hygiene, which needs to be treated as an important factor to enhance expertise related to major learning and improve students' living.

Breeding of New Silkworm Variety Golden silk, a Yellow Cocoon Color for Spring Rearing Season (황색실크 생산용 특수누에 품종 '골든실크' 육성)

  • Kang, Pil-Don;Lee, Sang-Uk;Jung, I-Yeon;Shon, Bong-Hee;Kim, Young-Soon;Kim, Kee-Young;Kim, Mi-Ja;Hong, In-Pyo;Lee, Kwang-Gill;Park, Kwang-Young
    • Journal of Sericultural and Entomological Science
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    • v.49 no.1
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    • pp.14-17
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    • 2007
  • A new silkworm variety Golden silk for spring rearing season is $F_1$ hybrid between Jam311, a Japanese race bred from introduction breeding and Jam312, a Chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2006, "Golden silk" was showed 7 percent higher in hatchability than authorized silk-worm races laval. "Golden silk" was receded higher 1.7 percent, 2.2 percent in pupation rate, best cocoon rate respectively than Chunsujam. The larval period shortened for 1.12 day even though low record to the several items including productivity than Chunsujam. "Golden silk" was showed 1,031 m, 2.97 denier in length of cocoon filament, weight of cocoon filament respectively. The concentration of Deoxynojirimycin(DNJ) was measured 4.34 mg in "Golden silk", high then to "Chunsujam"(3.81 mg). In the Paecilomyces tenuipes production ability test, the pupal weight of "Golden silk" was 1.20g.