• Title/Summary/Keyword: Extender

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Heterologous Expression of Hybrid Type II Polyketide Synthase System in Streptomyces Species

  • Kim, Chang-Young;Park, Hyun-Joo;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.819-822
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    • 2003
  • Polyketides are an extensive class of secondary metabolites with diverse molecular structures and biological activities. A plasmid-based minimal polyketide synthase (PKS) expression cassette was constructed using a subset of actinorhodin (act) biosynthetic genes (actI-orfl, actI-orf2, actI-orf3, actIII, actⅦ, and actIV) from Streptomyces coelicolor, which specify the construction of an orange-fluorescent anthraquinone product aloesaponarin II, a type II polyketide compound derived from one acetyl coenzyme A and 7 malonyl coenzyme A extender units. This system was designed as an indicator pathway in S. parvulus to generate a hybrid type II polyketide compound via gene-specific replacement. The act ${\beta}-ketoacyl$ synthase unit (actI-orfl and actI-orf2) in the expression cassette was specifically replaced with oxytetracycline ${\beta}-ketoacyl$ synthase otcY-orfl and otcY-orf2). This plasmid-based hybrid PKS cassette generated a novel orange-fluorescent compound structurally different from aloesaponarin II in both S. lividans and S. parvulus. In addition, several additional distinctive blue-fluorescent compounds were detected, when this hybrid PKS cassette was expressed in S. coelicolor B78 (actI-orf2 mutant), implying that the expression of plasmid-based hybrid PKS cassette in Streptomyces species should be an efficient way of generating hybrid type II polyketide compounds.

Synthesis and Emulsification of Polyurethane Anionomer (음이온성 폴리우레탄의 합성 및 에멀젼화에 관한 연구)

  • Ann, Choun-Kee;Jin, Je-Yong;Lee, Gyung-Won;Choi, Sei-Young
    • Elastomers and Composites
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    • v.34 no.5
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    • pp.399-406
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    • 1999
  • Polyurethane (PU) prepolymers were synthesized from polytetramethylene ether glycol (PTMG), 4,4'-diphenylmethane diisocyanate (MDI), toluene 2,4-diisocyanate (TDI) and isophoron diisocyanate (IPDI). After chain extention using dimethyol propionic acid (DMPA), aqueous polyurethane anionomers were prepared from triethyl amine (TEA) as a neutralizer. The effect of the content of chain extender and the degree of neutralization on the state of emulsification, adhesive strength, viscosity, glass transition temperature and physical properties of emulsion cast film were investigated using UTM, Viscometer and DSC.

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A Study on Radiologic Technologist's License System and Primary Pathway Education Curriculum in the United States American : Focused on One Case of College in Texas (미국 방사선사 면허제도와 기본 교육과정에 대한 고찰 : 텍사스주 일개 대학 사례를 중심으로)

  • Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.43 no.1
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    • pp.35-43
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    • 2020
  • The purpose of this study was to study on radiologic technologist's license system and primary pathway education curriculum in the United States American (USA), focused on one case of college in Texas. We were collected and analyzed through class participation at a community college in Tarrant, interviews with professors of radiologic science and clinical radiographers, field trips, an internet search, and literature reviews. As a result, first, the American radiologic technologists license system is composed of fifteen chapters, and the professional education courses for each field are being carried out through three courses of a primary pathway, a post primary pathway and a physician extender. Second, the primary pathway courses consisted the radiography, the radiation therapy, the nuclear medicine, the magnetic resonance imaging, the sonography. Third, the USA had about 30 times more clinical practice time than Korea. In clinical practice, students had done actually examination through X-ray exposure on patients. Last radiographers in the USA was able to perform intravenous injection of radiopharmaceutical agents on patient, so that he could perform rapid examination and efficient manpower operation. This study could be used as basic data for the globalization of radiologic technologists license system in Korea.

Investigation of the Korean Traditional Hobun Manufacturing Technique - Centering on Weathering Method - (전통 호분 제조기술 연구 - 풍화방법을 중심으로 -)

  • Kim, Soon-Kwan;Lee, Han-Hyoung;Kim, Ho-Jeong;Jeong, Hye-Young
    • 보존과학연구
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    • s.29
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    • pp.199-220
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    • 2008
  • Hobun(Oyster shell White) is a traditional material used as extender and white pigment from ancient times. The production method of it, however, has been discontinued. We have studied the traditional production method of Hobun by weathering oyster shell, which is one of the traditional ways for preparing Hobun. Reproduction study of manufacturing method of the discontinued traditional material is an important accomplishment of our research. Also this study provides solid background knowledge to stabilize the production and supply of Hobun for the cultural asset repairing materials. The result can be summarized as follows: The production process of Hobun by weathering method takes 5 steps - (1) weathering shells ${\rightarrow}$ (2) washing ${\rightarrow}$ (3) pulverization ${\rightarrow}$ (4) separating fine powder by submerging in water ${\rightarrow}$ (5) drying. The major aim in step (1) is to eliminate organic impurities. In the step (4), the fine particles smaller than $25{\mu}m$ are separated by extracting the supernatant from stirred suspension after heavy particles are submerged. Also, the soluble inorganic impurities can be eliminated through the powder submerge in 15 times water and stirring the suspension 6 hours and changing the water 3~4 times. The final products have high quality with 94.03, 0.52, 2.05 for L, a, b, less than $25{\mu}m$ particle size, fine resistance for discoloration by light and environmental pollution and good workability.

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Cure Reaction of Epoxy Resin System with MN/HQ (MN/HQ가 첨가된 에폭시 수지계의 경화반응 연구)

  • Chun, In-Sook;Shim, Mi-Ja;Kim, Sang-Wook
    • Applied Chemistry for Engineering
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    • v.5 no.6
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    • pp.967-974
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    • 1994
  • We studied the cure kinetics of the DGEBA(Diglycidyl ether of bisphenol A)/MDA(4, 4' -methylene dianiline)/MN(Malononitrile)/HQ(Hydroquinone) system by Barrett method and Integral method with dynamic runs of differential scanning calorimetry(DSC). Kinetic parameters such as activation energy and pre-exponential factor were obtained and reaction order was estimated roughly supposing that present system was adjusted to nth order reaction. The MN(Malononitrile) was introduced as a chain extender and HQ(Hydroquinone) as a reactive accelerator or catalyst.

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Study on the Synthesis of Polyurethane Cationomers and Their Mechanical Properties (양이온성 폴리우레탄의 합성 및 기계적 특성에 관한 연구)

  • Ann, Choun-Kee;Jin, Je-Yong;Choi, Sei-Yong
    • Elastomers and Composites
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    • v.33 no.3
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    • pp.177-184
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    • 1998
  • Polyurethane(PU) prepolymers were synthesized from polytetramethylene ether glycol(PTMG), with 4,4'-diphenylmethane diisocyanate(MDI), toluene 2,4-diisocyanate (TDI) and isophoron diisocyanate(IPDI). After chain extention using n-methyl-diethanol amine(n-MDEA), aqueous polyurethane cationomers were prepared by addition of glycolic acid(GA) as a quaternizer. The effect of the content of chain extender and the degree of neutralization on the stability of emulsion, adhesive strength, viscosity, glass transition temperature and physical properties of emulsion cast film were investigated using UTM, viscometer and DSC.

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Volumetric Interferometry Using Spherical Wave Interference for Three-dimensional Coordinate Metrology

  • Rhee, Hyug-Gyo;Chu, Ji-Young;Kim, Seung-Woo
    • Journal of the Optical Society of Korea
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    • v.5 no.4
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    • pp.140-145
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    • 2001
  • We present a new method of volumetric interferometer, which is intended to measure the three-dimensional coordinates of a moving object in a simultaneous way with a single optical setup. The method is based on the principles of phase-measuring interferometry with phase shifting. Two diffraction point sources, which are made of the polished ends of single-mode optical fibers are embedded on the object. Two spherical wavefronts emanate from the diffraction point sources and interfere with each other within the measurement volume. One wavefront is phase-shifted by elongating the corresponding fiber using a PZT extender. A CCD array sensor fixed at the stationary measurement station detects the resulting interference field. The measured phases are then related to the three-dimensional location of the object with a set of non-liner equations of Euclidean distance, from which the complete set of three-dimensional spatial coordinates of the object is determined through rigorous numerical computation based upon the least square error minimization.

A Study on Measurement of Mechanical Properties of Polyther Polyol Base Polyurethane Dispersion by 1,4-butanediol Chain Extension (Polyether Polyol을 이용한 수분산 폴리우레탄의 1,4-butanediol 사슬연장에 의한 물성변화)

  • Lee, Joo-Youb
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.4
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    • pp.711-718
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    • 2014
  • In this paper, the mechanical property change was investigated by adding 1,4-butanediol as chain extender of polyurethane dispersion for leather coating. Reagents for the synthesis were polypropylene glycol(PPG), isoporon diisocyanate(IPDI), dimethylolpropionic acid(DMPA) and 1,4-BD. All samples showed good solvent resistance and flexibility by different content of the 1,4-BD. As results, samples which contains a lot of 1,4-BD showed good properties whth strength of $1.80kg_f/mm^2$ and abrasion test measurement of 49.54 mg.loss. According to elongation test, the sample which contains low amount of 1,4-BD was good property as 364 %.

Morphological Analysis of Engineered PCC by Gas-Liquid Mixing Conditions (기체-액체 혼합조건에 따른 Engineered PCC의 형태학적 분석)

  • Lee, Tai-Ju;Seo, Jin-Ho;Kim, Hyoung-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.43 no.3
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    • pp.113-120
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    • 2011
  • Precipitated calcium carbonate(PCC), particularly calcite crystal, is extensively used as a pigment, filler or extender in various industries such as paper, paint, textile, detergents, adhesives, rubber and plastics, food, cosmetics, and biomaterials. PCC is conventionally produced through the gas-liquid carbonation process, which consists on bubbling gaseous $CO_2$ through a concentrated calcium hydroxide slurry. This study is aimed to find some factors for controlling the morphology of engineered PCC in lab-scaled mixing batch. The experimental designs were based on temperature variables, $Ca(OH)_2$ concentration, $CO_2$ flow rate, and electrical conductivity. The model of engineered PCC morphology was finally controlled by adjustment of electrical conductivity(6.0~7.0 mS/cm) and $Ca(OH)_2$ concentration(10 g/L). Orthorhombic calcite crystals were mostly created at high concentration and electrical conductivity conditions because the increased ratio of $Ca^{2+}$ and $CO{_3}^{2-}$ ions affects the growth rate of orthorhombic faces. Excess calcium spices were contributed to the growth of faces in calcium carbonate crystal, and the non-stoichiometric reaction was occurred between $Ca^{2+}$ and $CO{_3}^{2-}$ ions during carbonation process.

Use of Polyethylene as an Additive in Plywood Adhesive (합판 접착제의 첨가제로서 폴리에틸렌의 이용)

  • Oh, Yong-Sung
    • Journal of the Korean Wood Science and Technology
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    • v.26 no.1
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    • pp.14-18
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    • 1998
  • A low density polyethylene(LDPE) was examined as an additive in phenol-formaldehyde(PF) resin adhesive for bonding radiata pine plywood. The LDPE was supplied by the commercial manufacturer. The LDPE was compared to a commercial filler commonly used in structural plywood adhesives in the United States. The adhesive mixes were made by following the recommended procedure of Georgia-Pacific Resins Inc.. using plywood-type PF resin. A total of 48 three-ply plywoods. 6.3 mm nominal thickness and 30 by 30 em in size, were made at two press times (4 and 5 min). two press temperatures (150 and $160^{\circ}C$) and 30 minute assembly times for four adhesive mixing types. Evaluations of the LDPE addition were carried out by performance tension shear tests after two cycle boil aging tests on plywood per the U.S. Product Standard PS I-83. After accelerated-aging tests. plywoods were exhibited no delamination. The test results included tension shear strength and estimated wood failure values. The plywood test results support the use of polyethylene as an additive in plywood adhesives.

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