• Korean Journal of Agricultural Science
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• v.3 no.1
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• pp.121-134
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• 1976

There are many evidences that vaiious plant pathogenes were introduced with imported plants and agricultural productsrfrom foreign countries and caused heavy losses of domestic economic plants, ever since the early twentieth century when our country began to trade plants and agricultural products with foreign countries. There are many ways that foreign plant pathogenes have been introduced into our country, but the main route is considered to be imported plants and agricultural products contaminated with plant pathogenes. Plant quarantine which prevent effectively introduction of plant pathogenes from abroad was practiced for the first time in our country in 1912, and that is relatively earlier activity in the history of plant quarantine of the world. Several plant pathogenes have been introduced into our country even after plant quarantine had been practiced. Particularly for about 15 years, from the 2nd World War to 1961 when the law of Plant Protection was enacted and practical works of plant quarantine was reoperated, Korean agriculture was opened to various foreign plant pathogenes as a lapse period of plant quarantine in our country. Introduced plant pathogenes are, for the most part, from Japan because of depending upon Japan in the foreign trade of plants and agricultural products of our country. As present plant quarantine is required more exactness and rapidness, reasonable organization of quarantine system including more trained quarantine specialists, modernized facilities and introduction of improved quarantine techniques are necessary. Reasonable organization and improvement of plant quarantine system are important not only for protection of korean agriculture to plant pathogenes possible to be introduced from foreing countries, but also for increasing and stabilization of export of plants and agricultural product of our country.

• Korean journal of applied entomology
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• v.62 no.4
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• pp.333-343
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• 2023

King oyster mushrooms are one of the major fresh agricultural products which their exports are increasing every year in Korea. Lycoriella ingenua, is notorious insect pest in agriculture, especially in mushroom production. Larvae of L. ingenua cause mainly direct crop damage and adults are vectors of several dangerous fungal pathogens. In this study, the effects of electron beam, X-ray, and gamma-ray irradiation on the development and reproduction of L. ingenua were evaluated. In addition, to find the optimal dose to control L. ingenua in a box filled with king oyster mushrooms, an empirical experiment was conducted for each radiation. As a result, the development and reproduction of L. ingenua were inhibited at 50 Gy for all electron beam, X-ray, and gamma-ray irradiation. Additionally, at the top, middle, and bottom of the export box filled with king oyster mushrooms, the development and reproduction of L. ingenua were inhibited by electron beam with 150 Gy, X-ray with 100 Gy, and gamma-ray with 50 Gy. These results can be provided as basic data for establishing an integrated quarantine management system when exporting mushrooms. It will also contribute to the safety of agricultural products and the strengthening of export competitiveness.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.343-349
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• 2015

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most important bacterial diseases of citrus. Because citrus canker is not found in many countries including European Union and Australia, Xcc is strictly regulated in order to prevent its spread. In this study, the effects of X-irradiation on Xcc growth either in the suspension or on the surface of citrus fruits were investigated. The suspension containing $1{\times}10^7cfu/ml$ of Xcc was irradiated with different absorbed doses of X-irradiation ranging from 50 to 400 Gy. The results showed that Xcc was fully dead at 400 Gy of X-irradiation. To determine the effect of X-irradiation on quarantine, the Xcc-inoculated citrus fruits were irradiated with different X-ray doses at which Xcc was completely inhibited by an irradiation dose of 250 Gy. The $D_{10}$ value for Xcc on citrus fruits was found to be 97 Gy, indicating the possibility of direct application on citrus quarantine without any side sterilizer. Beside, presence of Xcc on the surface of asymptomatic citrus fruits obtained from citrus canker-infected orchards was noted. It indicated that the exporting citrus fruits need any treatment so that Xcc on the citrus fruits should be completely eliminated. Based on these results, ionizing radiation can be considered as an alternative method of eradicating Xcc for export of citrus fruits.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.30 no.1
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• pp.50-57
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• 2020

Objectives: Methyl bromide is a highly toxic substance that can cause systemic poisoning, neurotoxicity, pulmonary edema. Methyl bromide is a widespread fumigation agent used for import and export quarantine, but there is little data on exposure assessment of workers participating in such fumigation. Therefore, this study aims to investigate the exposure level of methyl bromide among workers and the concentration distribution of methyl bromide in the workplace during fumigation. Methods: For this study, one wood tent and one container fumigation area in Pusan, and one wood tent fumigation area in Incheon were investigated over three months from June 2001. Results: Methyl bromide exposure levels for container fumigation workers were from undetected to 1.86 ppm and from undetected to 2.28 ppm for tent fumigation workers as an 8 hr TWA. After opening the container door, the methyl bromide concentration in the container decreased rapidly over time, but the concentration remained over 1 ppm even after about one hour. The concentrations around the tent during its removal were highly affected by wind, but were detected in a range from undetected to 11.97 ppm. Conclusions: This study confirmed that both container and tent fumigation caused workers to exceed the exposure limit of 1 ppm as an 8 hour TWA. In particular, it could be confirmed that a situation exists in which the worker may be exposed to high concentrations of methyl bromide when opening the container or removing the tent due to the nature of the work. Therefore, it is very important to improve working methods and to wear appropriate personal protective equipment.

• Food Science of Animal Resources
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• v.18 no.2
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• pp.115-124
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• 1998

This study was carried out investigate hygienic conditions of domestic packing plant for export and the shelf-life by Japanese standards. vacuum packaged chilled port loins provided by five packers(A, B, C, D and E) were transported to Japan in the same condition, Four packers(A, B, C adn d) produced the samples as hygienic as possible and one packer produced loins in the traditional hygienic condition, In TTC(2,3,5-triphenyl tetrazolium chloride) test all samples showed the negative reaction from the date of the quarantine(14 days after slaughtering) to 50 days The numbers of total plate count(TPC) of A, B, C and D packers that were below 108CFU/g until 50 days were accepted by Japanese Standards but TPC of E packer was over 107CFU/g at 30 days. VBN values increased sig-nificantly(P<0.01) in samples of A,B and D packers in the samples of C packer and E packer at 5% and 0.1% level respectively. The pH values of the vacuum packaged chilled pork for export at 14 days after slaughtering were 5.43∼6.00 In sensory evaluation, A, B, C and D packers which improved hygi-enic condition produced the products with good color and appearance. low drip until 40 days storage at $0^{\circ}C$ But as the storage time increase off-flavor occured at 40days in A and D packer and at 50days in B and C packer. In conclusion according to the above results of microbiological physicochemical and sensory evalution the edible periods of Korean vacuum package chilled porks may be estimated to 40∼50 days for the packers who improved hygienic condition and the shelf lives were calculated as 32∼40 days from edible period by a factor of 0.8(safety coefficient) while that of the pork from E packer which was produced in the traditional hygienic condition was estimated to 30 days after slaughtering at $0^{\circ}C$ Therefore the shelf-life of the pork of E packer would be only 24 days.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.45-49
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• 2001

As a series of studies to develop pest management system for sweet persimmon production matched with the quarantine criterion of sweet persimmon-imported county, a survey was conducted by questionnaire on pest management practice and scheme of increasing income in sweet persimmon farms in Korea. Above 80% of sweet persimmon farmers was over fifties in age. The greatest limiting factor for sweet persimmon production was thought to be disease by the farmers. Percentage of farmers who answered that the most important pest is anthracnose cause by Gloeosporium kaki, and stink bugs was 64.6% and 73.5%, respectively. In particular, the farmers did not consider Dichocrocis punctiferalis, a main targer insect for quarantine in USA, as serious problem. Obtaining information on pest management practice or decision-making on both selection of pesticide and time of pesticide application depended highly on their own experience with the highest proportion. Eight to nine times of pesticide applications a year was highest among farms with 56.6%. More than 80% of farmers recorded the diary of pesticide application. farmers considered increasing cost for pest management as the greatest problem with 35.4% in proportion, and 34.5% of farmers pointed out that increase in export is essential for increasing farm income with highest proportion.

• Mycobiology
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• v.30 no.2
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• pp.105-106
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• 2002

The isolation of a causal pathogen from pear fruits showing lesions of blue mold was carried out, which were preserved in the packinghouse of Anseong for the export to the United States. When the lesion of pears(Pyrus pyrifolia Niitaka) was checked in packinghouse, their fruit epidermis over decayed parts varied from light tan to dark brown. Also, the decayed flesh was soft and watery, and separated easily from the healthy tissue. Based on the cultural and morphological characteristics, the fungus(IL-12 isolate) was identified as Penicillium aurantiogriseum. This is the first report associated with blue mold of pear caused by P. aurantiogriseum in Korea.

• Journal of People, Plants, and Environment
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• v.23 no.2
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• pp.171-178
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• 2020

The present study was conducted to determine the effects of electron beam irradiation on the postharvest quality of cut flowers. Cut flowers were irradiated with electron beam at 100, 200, 400, 600, 800, 1,000, and 2,000 Gy with a 10 MeV linear electron beam accelerator to evaluate their irradiation tolerance. Postharvest quality was determined by monitoring fresh weight loss, flower longevity, flower diameter, flowering rate, visual quality of flowers and leaves, and chlorophyll content. Cut flowers showed a radiation-induced damage with increasing the irradiation dose. Flower longevity and fresh weight of cut flowers decreased when the irradiation dose was increased. Flower bud opening was also inhibited in a dose-dependent manner. The effective irradiation doses for 10% reduction of postharvest quality (ED₁₀) values were 144.4, 451.6, and 841.2 Gy in the 'Medusa' lily, 'Montezuma' carnation, and 'Rosina White' eustoma, respectively. Although tolerance of cut flowers to electron beam irradiation vary according to species, cultivars, or maturity stage conditions, it is conceivable that 'Montezuma' carnation and 'Rosina White' eustoma could be tolerated and maintained overall postharvest quality up to 400 Gy, the generic irradiation dose approved by the Animal and Plant Health Inspection Service (APHIS) and the International Plant Protection Convention (IPPC) for postharvest phytosanitary treatments.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.228-237
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• 2003

Temporary control schedules were tested at sweet persimmon orchards to development new control programs to meet the quarantine repuirements of America in 2001 and 2002. The 'MRL-type control orchards' were sprayed with chemicals which were possibly adaptable to the pome trees in America. A control schedule consisted of those chemicals registered for persimmon in Korea was incorporated in the 'domestic-type control orchards'. The efficacy of these two control type against insect pests was compared with that of a conventional control schedule. In 2001, MRL orchard and domestic orchard were sprayed 7 and 6 times, and two conventional orchards were 6 and 9 times, respectively. In 2002, acaricide was added once to the MRL orchards at late September to reduce the density of mites on harvested fruits. However no insecticide to plant bug control could be applied to the MRL orchards, because no insecticide against bugs was registered for pome trees in America. This resulted in 7 times of applications in MRL and domestic orchards. The conventional orchard was sprayed 9 times. Only the occurrence of the peach pyralid moth, Dichocrocis puntiferalis (PPM) out of 4 quarantine inset species was observed. The PPM was observed during growing season in MRL, domestic, and conventional orchards. However no fruits damaged by PPM larvae were observed after mid October and after harvest. In 2002 only 1 fruit out of 1,350 fruits inspected in June was damaged by the larvae of PPM at MRL orchards. A fungus-feeding mites and collembolan were under calyx of vested fruits. In 2001 they were found on 45.3% of harvested fruits at MRL orchard. However the percentage of fruits with mites in 2002 was greatly reduced to 3.5% at MRL orchard, presumably because of a added application of acaricide at late September. However percentage of fruits damage by hemipteran bugs at harvesting time was quite high 11.3 % at MRL orchards, because no application of insecticide against plant bugs.

• Research in Plant Disease
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• v.18 no.3
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• pp.217-224
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• 2012

Post-harvest rot of grape causes a severe economic loss and lower of the grape quality. It is also one of the important limiting factors for grape export. Grape rots and their casual agents on 'Red Globe' variety imported from Chile were identified. Grapes shown rotting symptom were collected from the storages near the import harbor. The 3 different rots were identified on the imported 'Red Globe'; melting decay, gray mold, and blue mold. A bacterium that isolated from a typical melting decay symptom was identified as Gluconobacter cerinus on basis of its nucleotide sequence of 16S rDNA and fatty acid profile. By inoculation on grape, it caused cracking and dissolution of epidermis of grape which were the characteristics of melting decay. Botrytis cinerea and Penicillium expansum were isolated from grapes showing gray mold and blue mold. The 2 fungal isolates were identified on basis of their morphological characteristics and nucleotide sequence of their beta-tubulin genes. They showed strong pathogenicity on 'Campbell Early' variety that is a major table grape in Korea.