• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.80-80
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• 2003

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by marked cell death in the striatum and cortex. Stereotaxic injection of quinolinic acid (QA) into striatum results in a degeneration of GABAergic neurons and exhibits abnormal motor behaviors typical of the illness. The objective of this study was carried out to obtain basic information about whether parthenogenetic mouse embryonic stem (PmES) cells are suitable for cell replacement therapy of HD. To establish PmES cell lines, hybrid F1 (C57BL/6xCBA/N) mouse oocytes were treated with 7% ethanol for 5 min and cytochalasin-B for 4 hr to initiate spontaneous cleavage. Thus established PmES cells were induced to differentiate using bFGF (20ng/ml) followed by selection of neuronal precursor cells for 8 days in N2 medium. After selection, cells were expanded at the presence of bFGF (20 ng/ml) for another 6 days, then a final differentiation step in N2 medium for 7 days. To establish recipient animal models of HD, young adult mice (7 weeks age ICR mice) were lesioned unilaterally with a stereotaxic injection of QA (60 nM) into the striatum and the rotational behavior of the animals was tested using apomorphine (0.1mg/kg, IP) 7 days after the induction of lesion. Animals rotating more than 120 turns per hour were selected and the differentiated PmES cells (1$\times$10$^4$cells/ul) were implanted into striatum. Four weeks after the graft, immunohistochemical studies revealed the presence of cells reactive to anti-NeuN antibody. However, only a slight improvement of motor behavior was observed. By Nissl staining, cell mass resembling tumor was found at the graft site and near cortex which may explain the slight behavioral improvement. Detailed experiment on cell viability, differentiation and migration explanted in vivo is currently being studied.

• 한국어병학회지
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• 제18권3호
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• pp.293-300
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• 2005

본 연구는 우리나라 주요 양식어류인 조피볼락을 대상으로 bisphenol A가 혈액에 어떠한 영향을 끼치는지를 조사하였다. 실험어를 대상으로 어체중 kg 당 0.1, 1 및 10 mg BPA를 0, 3, 7 및 12일째에 복강주사 한 후, 15일째에 그 영향을 조사하였다. BPA에 노출된 조피볼락은 적혈구수와 헤모글로빈의 농도가 유의한 감소를 나타내었고, 혈청 클로라이드, 칼슘, 혈당, 빌리루빈 및 요소질소의 농도는 증가하는 경향을 나타내었고, 콜레스테롤은 감소하였다. 또한, 혈청 전이효소와 젖산탈수소효소의 활성은 증가하였다.

• 자원리싸이클링
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• 제24권2호
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• pp.29-35
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• 2015

폐 젤리충진 통신케이블로부터 고순도 구리회수를 위한 친환경 공정 및 장치 개발과 고부가 나노분말 제조까지의 업사이클링 공정을 위한 적용성 평가를 수행하였다. 고압분사 공정은 공업용수만을 용매로 사용한 친환경 고효율 기계적 방법으로써 전산유동해석을 통해 최적화하여 배치공정 장치를 제작, 실험적으로 검증하였으며 99.5% 이상의 높은 고순도 구리 회수율을 얻을 수 있었다. 재활용 구리의 고부가가치화를 위해 액중전기폭발법을 이용하여 나노분말을 제조하였으며, 이에 대한 기초적인 경제성 평가를 수행하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제15권6호
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• pp.339-344
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• 2011

Ulmus davidiana var. japonica Rehder (Urticales: Ulmaceae) (UD) is a tree widespread in northeast Asia. It is traditionally used for anticancer and anti-inflammatory therapy. The present study investigated the effect of an ethanol extract of UD on vascular tension and its underlying mechanism in rats. The dried root bark of UD was ground and extracted with 80% ethanol. The prepared UD extract was used in further analysis. The effect of UD on the cell viability, vasoreactivity and hemodynamics were investigated using propidium iodide staining in cultured cells, isometric tension recording and blood pressure analysis, respectively. Low dose of UD ($10{\sim}100{\mu}g/ml)$ did not affect endothelial cell viability, but high dose of UD reduced cell viability. UD induced vasorelaxation in the range of $0.1{\sim}10{\mu}g/ml$ with an $ED_{50}$ value of $2{\mu}g/ml$. UD-induced vasorelaxation was completely abolished by removal of the endothelium or by pre-treatment with L-NAME, an inhibitor of nitric oxide synthase. UD inhibited calcium influx induced by phenylephrine and high $K^+$ and also completely abolished the effect of L-NAME. Intravenous injection of UD extracts (10~100 mg/kg) decreased arterial and ventricular pressure in a dose-dependent manner. Moreover, UD extracts reduced the ventricular contractility (+dP/dt) in anesthetized rats. However, UD-induced hypotensive actions were minimized in L-NAME-treated rats. Taken together, out results showed that UD induced vasorelaxation and has antihypertensive properties, which may be due the activation of nitric oxide synthase in endothelium.

• 한국식품영양과학회지
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• 제34권9호
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• pp.1362-1366
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• 2005

홍삼청국장 추출물(RC)이 알코올을 섭취중인 당뇨흰쥐 혈당과 혈청지혈에 미치는 효과를 관찰하기 위하여 SD계 흰쥐에 streptozotocin(STZ)을 투여하여 고혈당과 고지혈증을 유발시킨 다음 홍삼청국장 추출물을 함유(g/mL)하는 $20\%$ 알코올을 매일 2 mL씩 2주간 경구투여 하였을 때의 증체량, 식이효율, 음용수 섭취량과 배뇨량, 장기중량, 혈당 및 혈청지질 함량에 미치는 영향을 조사하였다. 그 결과 RC의 식이는 STZ 투여에 의하여 증가되었던 음용수 섭취량과 배뇨량을 개선시키지 못하였으나, 감소하였던 증체량과 식 이 효율은 대조군 수준으로 회복시켰으며, 고혈당과 고지혈증도 유의하게 감소시켰다. RC는 당뇨 유발 후 에탄을 투여로 인한 사망률을 현저히 감소시켰다. 이러한 결과와 문헌상의 지견들을 종합해 볼 때, RC는 고혈당 및 고지혈증을 치료 및 예방함으로서 에탄을 섭취에 의한 사망률을 감소시킴과 동시에 당뇨에 의해 발생할 수 있는 당뇨 합병증을 예방 내지는 지연시켜주는 작용이 있을 것으로 생각된다.

• Archives of Pharmacal Research
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• 제28권3호
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• pp.335-342
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• 2005

The effects of ginsenosides Rg$_3$(R) , Rg$_3$(S) and Rg$_5$/Rk$_1$ (a mixture of Rg$_5$ and Rk$_1$ 1:1, w/w), which are components isolated from processed Panax ginseng C.A. Meyer (Araliaceae), on memory dysfunction were examined in mice using a passive avoidance test. The ginsenosides Rg3(R), Rg3(S) or Rg$_5$/Rk$_1$, when orally administered for 4 days, significantly ameliorated the memory impairment induced by the single oral administration of ethanol. The memory impairment induced by the intraperitoneal injection of scopolamine was also significantly recovered by ginsenosides Rg3(S) and Rg$_5$/Rk$_1$. Among the three ginsenosides tested in this study, Rg$_5$/Rk$_1$ enhanced the memory function of mice most effectively in both the ethanol­and scopolamine-induced amnesia models. Moreover, the latency period of the Rg$_5$/Rk$_1$­treated mice was 1.2 times longer than that of the control (no amnesia) group in both models, implying that Rg$_5$/Rk$_1$ may also exert beneficial effects in the normal brain. We also evaluated the effects of these ginsenosides on the excitotoxic and oxidative stress-induced neuronal cell damage in primary cultured rat cortical cells. The excitotoxicity induced by glutamate or N­methyl-D-aspartate (NMDA) was dramatically inhibited by the three ginsenosides. Rg$_3$(S) and Rg$_5$/Rk$_1$ exhibited a more potent inhibition of excitotoxicity than did Rg$_3$(R). In contrast, these ginsenosides were all ineffective against the H$_2$O$_2$- or xanthine/xanthine oxidase-induced oxidative neuronal damage. Taken together, these results indicate that ginsenosides Rg$_3$(S) and Rg$_5$/Rk$_1$ significantly reversed the memory dysfunction induced by ethanol or scopolamine, and their neuroprotective actions against excitotoxicity may be attributed to their memory enhancing effects.

• 한국식품영양과학회지
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• 제36권8호
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• pp.955-959
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• 2007

메밀싹을 건조 후 세절하여 70% 에탄올을 가하여 추출한 추출물과 용매의 극성에 따라 분별분리를 행하여 핵산, 클로로포름, 에틸아세테이트, 부탄올 및 물 분획물로 조제하여 여섯 가지의 추출물 및 분획물을 얻었다. 이들 시료들에 대하여 항산화활성을 측정한 결과 에틸아세테이트 분획물에서 $RC_{50}$ 값이 26.1 ${\mu}g/mL$의 강한 항산화활성을 나타내었다. 염색체 유발 물질인 MNNG(N-methyl-N'-nitro-N-nitro-soguanidine)를 마우스에 50, 100, 150 그리고 200 mg/kg으로 투여한 경우의 소핵생성은 각각 3.7${\pm}$0.7, 4.3${\pm}$0.6, 12.8${\pm}$0.8 그리고 15.6${\pm}$1.1로써 농도증가에 비례적으로 증가하였으며 대조군에서는 1.4${\pm}$0.5의 소핵생성을 나타내었다. MNNG를 150 mg/kg과 메밀싹 추출물을 각각 10, 20, 40 그리고 80 mg/kg으로 동시에 투여한 경우 각각 23.4, 40.6, 56.3 그리고 73.4%의 소핵생성 억제효과를 나타내었다 . 메밀싹 용매 분획물 실험에서는 핵산, 클로로포름, 에틸 아세테이트, 부탄올 그리고 물 분획물이 시료농도 80 mg／kg의 투여군에서 양성대조군에 비해 각각 64.1, 67.9, 75.8, 74.2 및 63.3%의 소핵생성 억제율을 나타내었다. 따라서 메밀싹은 건강식품으로서의 개발가능성을 가진 매우 유용한 원료임을 알 수 있었고, 추후 이러한 생리활성을 나타내는 부분만을 분리, 정제하여 추가적인 검색 및 활용방안에 대하여 충분한 연구가 이루어져야 한다고 사료된다.

• Journal of Ginseng Research
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• 제27권2호
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• pp.56-61
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• 2003

복강 내당능 시험과 MLD STZ 당뇨를 유발시킨 rat에서 백삼, 홍삼, 화기삼의 항당뇨 활성을 비교한 결과를 요약하면 다음과 같다. 1. 복강 내당능 시험에서 백삼은 효과를 나타내지 못한 반면 홍삼과 화기삼은 대조군에 대하여 유의적인 변차를 나타내었다. MLD STZ로 당뇨를 유발시킨 SD계 흰쥐에 백삼, 홍삼, 화기삼을 2주간 투여 후 혈당강하 활성을 비교한 결과 홍삼 투여군의 혈당이 가장 낮았으며 백삼과 화기삼은 비슷한 크기의 활성을 나타내었다. 2. 백삼과 홍삼, 화기삼은 모두 고혈당에 의한 체중 감소 현상을 개선시켰으며 각 투여군 간에는 유의적인 차이는 없었다. 3. 당뇨의 대표적인 증상인 다식 (多食)과 다음 (多飮)현상이 백삼, 홍삼, 화기삼 투여에 의하여 개선되었으며 그 중 홍삼에 의한 효과가 가장 컸다. 4. 혈당강하 활성과 ginsenoside profile간의 상관관계를 살펴본 결과 PPT/PPD ratio가 가장 큰 홍삼의 혈당강하 활성이 백삼과 화기삼에 비해 높은 것으로 보아 PPT/PPD ratio가 혈당강하 활성의 key ratio가 아닌가 추측된다.

• Food Science and Biotechnology
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• 제14권4호
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• pp.456-460
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• 2005

To enforce the maximum residue limit for residual hexane (0.005 g/kg) in commercially available Korean vegetable oil, convenient and accurate quantification methods were investigated. Using dual surrogate standards, pentane and heptane were dissolved in ethanol, and then added to hexane-tree sunflower oil for setting up the calibration curve. Gas Chromatograph-Flame Ionization Detector with a porous layer open tubular column, indicated good chromatographic separation of hexane from other inhibiting matrix components. The lowest calibration level was $0.5\;{\mu}g/g$, not exceeding a relative standard deviation of 10% (RSD%), and 1.0\;{\mu}g/g$ not exceeding a deviation of 22% RSD% using heptane as an internal standard for the Static headspace analysis by using a headspace auto-sampler and manual injection, respectively. The residual hexane was detected in nine of the samples among 87 vegetable oil samples purchased on the local market.

• 생약학회지
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• 제47권4호
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• pp.360-365
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• 2016

The aim of this study was to compare the amount of spinosin in the 70% ethanol extracts of non-processed Zizyphi Semem (ZS) and processed ZS by roasting using a high-performance liquid chromatography equipped with photodiode array detector. Separation of the spinosin was used $SunFire^{TM}$ $C_{18}$ analytical column ($5{\mu}m$, $4.6{\times}150mm$) using two mobile phase consisting of distilled water and acetonitrile, both with 1.0% (v/v) acetic acid. The flow rate was 1.0 mL/min and injection volume was $10{\mu}L$. Calibration curve of the spinosin was y = 22339.45x+483.99 in tested concentration range ($1.28-20.00{\mu}g/mL$) and correlation coefficient was 1.0000. In non-processed ZS sample, the amount of the spinosin was 0.94 m/g, while, the amount of the marker compound in processed ZS samples were 0.66-1.10 mg/g.