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http://dx.doi.org/10.3746/jkfn.2007.36.8.955

Antioxidation and Antigenotoxic Effects of Buckwheat Sprout Extracts  

Kim, Su-Hyun (School of Bioscience and Biotechnology, Kangwon National University)
Lee, Eue-Yong (School of Bioscience and Biotechnology, Kangwon National University)
Ham, Seung-Si (School of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.36, no.8, 2007 , pp. 955-959 More about this Journal
Abstract
This study was carried out to determine the antioxidative and antigenotoxic effects of buckwheat (Fagopyrum esculentum Moench) sprout using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical donating method and micronucleus test. Buckwheat sprout were extracted with 70% ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate (EtOAc), butanol and water. Among the five fractions, the EtOAc fraction showed the highest electron donating activity ($RC_{50}$ 26.1 ${\mu}g/mL$). The effects of buckwheat sprout extracts on the frequencies of micronucleated polychromatic erythrocytes (MNPCEs) induced by MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) were investigated in the bone marrow. 10, 20, 40 and 80 mg/kg of each extract were administered to animals immediately after injection of MNNG and the exposure time was 36 hrs. Inhibition effects of buckwheat sprout ethanol extract were 23.4%, 40.6%, 56.3% and 73.4%, respectively. When the fraction of hexane, chloroform, ethyl acetate, butanol and water from 70% ethanol extract were treated with concentration of 80 mg/kg, the suppression rates of the MNPCE were 64.1, 67.9, 75.8, 74.2 and 63.3%, respectively.
Keywords
buckwheat sprout; antigenotoxic effect; micronucleus test; antioxidative effect;
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Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By SCOPUS : 2
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