• Title/Summary/Keyword: Ethanol extraction

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Evaluation on Extraction Conditions and HPLC Analysis Method for Bioactive Compounds of Astragali Radix (황기의 추출조건 및 유효성분의 HPLC 분석법 평가)

  • Kim, Geum Soog;Lee, Dae Young;Lee, Seung Eun;Noh, Hyung Jun;Choi, Je Hun;Park, Chun Geun;Choi, Soo Im;Hong, Seung Jae;Kim, Seung Yu
    • Korean Journal of Medicinal Crop Science
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    • v.21 no.6
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    • pp.486-492
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    • 2013
  • This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at $85^{\circ}C$ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as $27.27{\pm}2.27%$, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of $10.55{\pm}0.24%$. The quantitative determination methods of calycosin-7-O-${\beta}$-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column ($4.6{\times}250mm$, $5{\mu}m$) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of $0.8mLmin^{-1}$ and a detection wavelength of 230nm. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-${\beta}$-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as $1,700.3{\pm}30.4$ and $443.6{\pm}8.4{\mu}g-1$, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at $85^{\circ}C$ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

Effect of the Extracting Condition on the Crude Fat and Free Fatty Acids of Red Ginseng Extract (추출조건이 홍삼엑기스의 지방과 유리지방당 조성에 미치는 영향)

  • 성형순;윤석권
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.179-185
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    • 1985
  • The effect of temperature and ethanol concentration on lipids yield and free fatty acids were investigated during 1-5 times of 8 hours extraction. The crude lipids recovered was generally increased as the ethanol concentration increased while it was decreased at higher extraction temperature. Fatty acids composition in free fatty acids showed linoleum and plasmatic acids to be the major fatty acids, accounting 60% of 16 fatty acids identified. Fatty acids pattern was little affected by the changes in ethanol concentration while increase in temperature resulted significant changes in fatty acids composition.

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Extraction of Indole Alkaloids from Catharanthus roseus by Using Supercritical Carbon Dioxide with Ethanol (초임계 이산화탄소와 에탄올을 이용한 일일초로부터 인들 알칼로이드의 추출에 관한 연구)

  • 송규민;박상우
    • KSBB Journal
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    • v.6 no.4
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    • pp.407-412
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    • 1991
  • The extraction of indole alkaloids from Catharanthus roseus using the supercritital carbon dioxide with ethanol was performed in various temperature and pressure conditions. The contents of vindoline in the extracts were determined by HPLC. In this study, the flow rate of cosolvent ehtanol was 20.4$\mu$l/min, about 3 wt.% of flow rate of supercritical carbon dioxide. However the effect of ethanol on the yield of extracted vindoline was not significant.

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Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage

  • Shin, S.C.;You, S.J.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.4
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    • pp.601-604
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    • 2006
  • The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

Effect of Extraction Procedures on Chemical Composition and Physical Properties of Lycii Cortex (추출조건(抽出條件)이 지골피(地骨皮)의 화학성분(化學成分) 및 물리적(物理的) 특성(特性)에 미치는 영향(影響))

  • Park, Jong-Sang;Park, Jun-Hong;Lee, Bong-Chun
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.2
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    • pp.91-95
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    • 1998
  • Some physical properties and chemical compositions of Lycii Cortex depending on the various extracting conditions were investigated for their changes during extraction with ethanol and water. Solid matter and contents of total sugar in water extracts were higher than those of ethanol extract. but contents of tannic acid in ethanol extracts was higher than that in water extracts. The methanol extract of the Lycii Cortex were fractionated with ethyl ether. ethyl acetate. n-butanol and water. The yield was higher in the order of water > n - butanol > ethyl ether and ethyl acetate fraction. Turbidity of water extraction was higher than that of ethanol extraction.

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Supercritical Fluid Extraction of Catecnin Compounds from Green Tea (녹차에서 카테킨 화합물의 초임계 유제 추출)

  • 나영진;이윤우;김재덕;노경호
    • KSBB Journal
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    • v.16 no.4
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    • pp.327-331
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    • 2001
  • Catechin compounds from the Green tea cultivated in Bosung (Chollanamdo) were extracted using supercritical fluid and various additives, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was packed with LiChrospher 100RP-18(15 $\mu$m), and water was used as the mobile phase with 0.05% phosphoric acid and acetonitrile. Gradient election was applied to separate EGCG by changing the mobile phase compositions. Comparing the extraction yield of three different types of supercritical fluids, pure CO$_2$, with additives of water and ethanol (5 wt,%), the extraction amount of EGCG was most abundant in the suprcritical CO$_2$with ethanol. However, more was extracted and pure higher purity was achieved by solvent extraction using ethanol.

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Some Physical Properties of Koran Red Ginseng Extract as Affected by Extracting Conditions (추출조건에 따른 홍삼(紅蔘)엑기스의 물리적 성질의 변화)

  • Sung, Hyun-Soon;Kim, Na-Mi;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.18 no.3
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    • pp.241-244
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    • 1986
  • Some physical properties of Korean red ginseng extract was investigated for their changes during extraction with ethanol solution having a concentration range of 0-90% at $70-100^{\circ}C$ during 5 times of 8 hours extraction. The intrinsic viscosity was ignificantly decreased as the ethanol concentration and measuring temperature increased, while it was affected relatively less by the extraction temperature. However the effect of measuring temperature on the intrinsic viscosity was greatly reduced for the extract obtained with higher than 50% a ethanol. The turbidity and pH were also decreased as the ethanol concentration and extraction time increased.

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Optimization of Production Yield for Neohesperidin by Response Surface Methodology (반응표면 분석법을 이용한 neohesperidin 생산 수율의 최적화)

  • Yang, Hee-Jong;Jeong, Seong-Yeop;Choi, Nack-Shick;Ahn, Keug-Hyun;Park, Chan-Sun;Yoon, Byoung-Dae;Ryu, Yeon-Woo;Ahn, Soon-Cheol;Kim, Min-Soo
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1691-1696
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    • 2010
  • Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determinedusing response surface methodology (RSM) in order to increase the neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure ($x_1$), extraction time ($x_2$), and concentration of ethanol ($x_3$). As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.

Polyphenol Contents of Rumex crispus Root Extract with Hot Water and its Antioxidative Effect (소리쟁이 뿌리 열수 추출물의 폴리페놀 함량 및 추출물의 항산화 효과)

  • Yun, Young-Sim;Jeong, Kap-Seop
    • Journal of Environmental Science International
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    • v.21 no.10
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    • pp.1265-1274
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    • 2012
  • This study was conducted to investigate the extraction characteristics including total polyphenol compound content (TPC) and the antioxidative effect of Rumex crispus root extract on commercial corn oil. Extraction yield was increased with extraction temperature but decreased with extraction ratio. No significant differences in aromatics content were found among the extracts prepared with various extraction conditions; extraction ratio, extraction temperature, extraction pH and composition of extractant. Total flavonoid content of the extract was increased with extraction temperature and extraction pH, and highest value of it was found when extractant composition of ethanol in water was 75%. Total polyphenol compound content (TPC) of the extract with 10 fold extraction ratio showed the highest value, but no significant difference in TPC was found with extraction temperature. Reducing power and DPPH (${\alpha}$,${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl) radical scavenging ability (RSA) of the extract in spectrophotometric absorbance were decreased with extraction ratio but increased with extraction temperature and showed 63.1%~98.4% and 67.6%~86.7% of those of ascorbic acid in reducing power and DPPH RSA, respectively. The antioxidative effects of the extract on corn oil were 84.8~93.0% of that of commercial butylated hydroxytoluene, the antioxidant index value was highest when the ethanol composition to water in extractant was equal ratio.

Antioxidant Activities of Extracts from Different Parts of the Pine Tree (소나무 부위별 추출물의 항산화 활성)

  • Ryu, Beom-Seok;Choi, Hee-Eun;Choi, Won-Seok;Lee, Nan-Hee;Choi, Ung-Kyu
    • The Korean Journal of Food And Nutrition
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    • v.30 no.6
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    • pp.1133-1139
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    • 2017
  • This study was conducted to investigate the antioxidant activities of extracts from various parts of the pine tree, which is known as a good source of functional food material. While ethanol extraction yields of pine bud and cone were higher than water extraction yields of pine bud and cone, water extraction yield of pine needle was higher than ethanol extraction yield of the pine needle. The content of polyphenols in the pine cone ethanol extract was 5 times higher than that in the pine bud and needle. Further, the content of flavonoids in the pine cone ethanol extract was 8 times higher than that in the pine bud and needle. DPPH radical scavenging effect of the pine cone ethanol extract was 3~5 times higher that of the pine bud and needle extract. Regardless of the extraction solvents, trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) of the pine cone were stronger than those of the other parts of the pine tree. Taken together, it can be expected that the pine cone can be practically used as an antioxidant substance in food and beauty industries.