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http://dx.doi.org/10.5352/JLS.2010.20.11.1691

Optimization of Production Yield for Neohesperidin by Response Surface Methodology  

Yang, Hee-Jong (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Jeong, Seong-Yeop (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Choi, Nack-Shick (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Ahn, Keug-Hyun (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Park, Chan-Sun (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Yoon, Byoung-Dae (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
Kim, Min-Soo (Bioindustrial Process Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Life Science / v.20, no.11, 2010 , pp. 1691-1696 More about this Journal
Abstract
Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determinedusing response surface methodology (RSM) in order to increase the neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure ($x_1$), extraction time ($x_2$), and concentration of ethanol ($x_3$). As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.
Keywords
Neohesperidin; response surface methodology; citrus unshiu; central composite design;
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