• Title/Summary/Keyword: Estrogenic study

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Study on Estrogenic Activities of Pesticide Chemicals using E-screen Assay (E-Screen assay법을 이용한 농약화학물질의 에스트로겐 활성 연구)

  • Han, Sang Guk
    • Journal of Environmental Science International
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    • v.13 no.6
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    • pp.591-597
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    • 2004
  • In this study, sixty chemicals including 47 pesticides were screened for estrogenic activity using E-screen assay. MCF-7 cell, used in E-screen assay, is known to be proliferated by addition of estrogenic chemicals. Eight of the measured pesticides showed estrogenic activity at the concentration range of 100-10,000nM. Their relative proliferative effect (RPE) and the relative proliferative potency (RPP) were 20-65% and 0.01-1.0%, respectively, when compared with 1.0nM of $\beta-Estradiol-17-acetate(E_{2}).$ DDVP and diazinone showed most strong estrogenic potency(RPP; 1.0%) and effect(RPE; 65%) of the eight pesticides. These results are in agreement with estrogenic activity of bisphenol A is known as a positive endocrine disrupter. Also, in this study, paraquat, DDVP, 4-chloro-3-methylphenol, 2,4,6-trichlorophenol and diazinon of the measured pesticides are estimated to estrogenic chemical.

Antifertility activity of hydro alcoholic extract of Moringa concanensis Nimmo: An ethnomedicines used by tribals of Nilgiris region in Tamilnadu

  • Ravichandiran, V;Suresh, B;Sathishkumar, MN;Elango, K;Srinivasan, R
    • Advances in Traditional Medicine
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    • v.7 no.2
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    • pp.114-120
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    • 2007
  • In the present study, the hydro alcoholic extract of Moringa concanensis and their different fractions were evaluated for it's anti implantation, abortifacient, estrogenic and antiestrogenic activity. Hydro alcoholic extract of Moringa concanensis has showed potent antiimplantation and abortifacient activity at 200 mg/kg and 400 mg/kg respectively and marked estrogenic activity when administered individually and anti estrogenic activity was observed when administered along with ethinyl estradiol (1 ${\mu}g/rat/day$) as well as their different fractions of Moringa concanensis showed significant antiimplantation and abortifacient activity at 100 mg/kg. Moreover, all tested fractions showed significant anti estrogenic activity when administered simultaneously with ethinyl estradiol.

Reduction of Estrogenic Activity by Gamma-ray Treatment (감마선 처리에 의한 에스트로겐 활성 저감 연구)

  • Kang, Sung-Wook;Seo, Jaehwan;Lee, Byoung Cheun;Kim, Suejin;Jung, Jinho
    • Journal of Korean Society on Water Environment
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    • v.26 no.6
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    • pp.948-953
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    • 2010
  • In this study, degradation of estrone (E1) and $17{\alpha}$-ethynylestradiol (EE2) by gamma-irradiation and subsequent reduction of estrogenic activity as a function of absorbed dose were conducted using the yeast two-hybrid assay. Relative potency of E1 and EE2 compared to estrogenic activity of $17{\beta}$-estradiol (E2) was found to be 0.0144 and 0.1605, respectively. More than 90% of E1 and EE2 (both $5.0{\times}10^{-6}M$) was removed at an absorbed dose of 5 kGy, but more than 40% of estrogenic activity still remained. The addition of $TiO_2$ catalyst appeared to improve the removal efficiency of E1 and decrease estrogenic activity while there was no significant effect for EE2. Additionally, the calculated estrogenic activity of E1 and EE2 based on a regression model was well correlated with the observed activity.

Study on Estrogenic Activities of Phthalate Esters Using E-screen Assay and Competitive Binding Assay (E-screen Assay 및 상경적 결합반응을 이용한 Phthalate Esters의 내분비계 장애 작용 연구)

  • 한순영;한상국;문현주;김형식;이동하;김소희;김태성;박귀례
    • Toxicological Research
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    • v.16 no.2
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    • pp.141-146
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    • 2000
  • Phthalate esters are used extensively as a plasticizer in the manufacture of plastic products such as PVC bags and medical devices. Recently, phthalate esters have been shown to induce endocrine system mediated responses. However. only a Jew studies have been conducted for estrogenic activity of phthalate esters. In this study estrogenic activities of seven phthalate esters. butyl benzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), di-n-butylphthalate (DBP), diethylphthalate (DEP), di-n-pentylphthalate (DPP), di-n-propylphthalate (DPrP) and dicyclohexylphthalate (DCHP), were examined in vitro using E-screen assay and competitive binding assay. From the E-screen assay, BBP. DEHP. DBP and DEP showed weak estrogenic activity at the concentration of 5 $\mu\textrm{M}$. The relative proliferative effect (RPE) and the relative proliferative potency (RPP) were 50~70% and 0.01%. respectively, when compared with 500 pM of 17$\beta$-estradiol (E2). In competitive binding assay with the rat uterine estrogen receptor (ER), BBP and DEP showed weak binding potency [(l/$10^4$~1/$10^5$ of E2] while DEHP and DBP scarcely bound to ER. These results suggest that some phthalate esters have weak estrogenic activities in vitro.

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No Estrogenic Activity of Phthalate Esters in Ovariectomized Rat Uterotrophic Assay (랫드 자궁비대반응시험(Uterotrophic assay)을 이용한 phthalate esters의 에스트로겐성 작용 연구)

  • 한순영;문현주;김형식;김철규;신재호;오세동;장성재;박귀례
    • Biomolecules & Therapeutics
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    • v.8 no.2
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    • pp.147-152
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    • 2000
  • The rodent uterotrophic assay is currently recommended as one of the primary in vivo assays far endocrine disrupting chemicals by the Organization for Economic Cooperation and Development (OECD) and Endocrine Disruptor Screening and Testing Advisory Committee (US EPA EDSTAC). Generally, this assay relies on the rapid increase in uterus and vagina weights when exposed to estrogenic compounds. Phthalate esters have been used extensively as a plasticizer in the manufacture of plastic products such as PVC films and medical devices. Recently, phthalate esters have been shown to induce endocrine system mediated responses. However, a flew studies have been conducted for the screening of their estrogenic activity. In this study the estrogenic activity of seven phthalate esters, butyl benzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), di-n-butylphthalate (DBP), diethylphthalate (DEP), di-n-pentylphthalate (DPF), di-n-propylphthalate (DPrP) and dicyclohexylphthalate (DCHP), was examined in uterotrophic assay. Phthalate esters dissolved in corn oil were administered to ovariectomized (OVX) female Sprague-Dawley rats by sub-cutaneous injection for three consecutive days. fiats were sacrificed 24h after final treatment, and then uterus and vagina weights were deter mined. All phthalate esters tested in this assay did not change talc uterus and vagina weights at dosage levels up to 200 mg/kg/day treatment. These results demonstrated that phthalate esters did not exhibit estrogenic activity in vivo uterotrophic assay.

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Assessment of the Estrogenicity of Isoflavonoids, Using MCF-7-ERE-Luc Cells

  • Joung, Ki-Eun;Kim, Yeo-Woon;Sheen, Yhun-Yhong
    • Archives of Pharmacal Research
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    • v.26 no.9
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    • pp.756-762
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    • 2003
  • In the current study, our research focused on the estrogenic activity of isoflavonoids, mainly genistein, biochanin A and daidzein. Genistein enhanced the reporter gene expression of MCF-7-ERE-Luc cells, at a concentration as low as 10 nM, with a concentration of 100 nM the achieved gene expression effects were similar to those of 10 pM 17$\beta$-estradiol. Based on the estrogenic activities of biochanin A and daidzein, hydroxyl groups at the 4 and 5 positions are needed for the maximal effect of the genistein. The estrogenic effects of these isoflavonoids were inhibited by the concomitant treatment with tamoxifen. The data showed that the estrogenic effects of isoflavonoids were mediated through estrogen receptors. When the isoflavonoids were tested as mixtures, the estrogenic effects were lower than the arithmetic sum of those induced by each individual isoflavonoid. The estrogenic potency of each isoflavonoid was presented at EC50 levels with a 17$\beta$-estradiol equivalent concentration (EEQ) based on the dose response of each chemical. The EC50s and EEQs of genistein, biochanin A and daidzein were 4.15, 0.89 and 0.18 $\mu$M, and 15.0, 5.12 and 1.83 $\mu$ M/M, respectively. Our data clearly demonstrated that the pERE-luciferase reporter gene assay was suited for the sensitive and quantitative measurement, and large scale screening, of the estrogenicity of chemicals in vitro.

Evaluation of Estrogenic Activity of Pumpkin Seed Extract using Recombinant Yeast Assay (재조합 효모를 이용한 호박씨 추출물의 에스트로겐 활성 평가)

  • Tahk, Hong-Min;Lee, Bog-Hieu;Rho, Sook-Nyung;Kim, Chun-Soo;Jung, Ji-Youn;Choi, Chang-Sun
    • Journal of Food Hygiene and Safety
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    • v.24 no.2
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    • pp.124-127
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    • 2009
  • The aim of this study is to evaluate the estrogenic activity of Cucurbita pepo seed extract which includes ${\beta}-sitosterol$ and other phytosterols. Sample was extracted from Cucurbita pepo seed by supercritical carbondioxide method and resuspended with ethanol. Estrogenic activity was measured by recombinant yeast assay which detects estrogenic activity using recombinant yeast with high level of estrogenic receptor. However, estrogenic activity of pumpkin seed extract was not found in this study. Based on this data, pumpkin seed extract will not cause estrogenic disturbance.

Estrogenic Activity of Leguminosae Species in Korea using MCF-7 Cells (유방암 세포주를 이용한 국내 자생 콩과식물의 에스트로겐 활성검색)

  • Bae, Ji-Yeong;Kim, Hye-Jin;Park, Woo Sung;Ahn, Mi-Jeong
    • Korean Journal of Pharmacognosy
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    • v.52 no.2
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    • pp.118-125
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    • 2021
  • Leguminosae plants are known for its phytoestrogen constituents which play a major role in the prevention of osteoporosis, cancer and heart disease. In this study, the estrogenic activity of 158 samples from 58 species, 3 subspecies and 10 varieties of Leguminosae plants growing in Korea was evaluated. An estrogen, 17β-estradiol was used as a reference compound, and the potency of each sample was expressed in relative efficacy (%) compared to that of the reference by a reporter gene assay using MCF-7 cells. As results, the estrogenic activity of methanolic extracts of Phaseolus vulgaris var. humilis, Sophora flavescens, Lespedeza × robusta, Indigofera pseudotinctoria, Maackia amurensis, Glycine soja, Wisteria floribunda, Robinia pseudoacacia, Astragalus sinicus, Pueraria lobata, Lespedeza maximowiczii var. tomentella, Trifolium repens and Crotalaria sessiliflora showed similar to or higher at 100 ㎍/ml than the positive control at 10 nM. These findings can be a potential evidence for developing estrogen alternatives resolving various types of menopause symptoms with information on proper harvest season and usage plant part. To the best of our knowledge, the estrogenic activity of Lespedeza × robusta, Indigofera pseudotinctoria, Wisteria floribunda, Robinia pseudoacacia and Lespedeza maximowiczii var. tomentella is reported for the first time in this study.

Studies on Pullorum Disease II. The Effect of Cortisone Acetate, Estrogenic Hormone and Tocopherol on the Diagnosis of Reactors of Pullorum Disease (닭의 추백리병에 관한 연구 II. 추백리병 진단에 있어서 Hormone 효과에 관한 연구)

  • Choi, J.Y.;Lee, S.Y.;Lee, C.K.
    • Korean Journal of Veterinary Research
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    • v.10 no.2
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    • pp.7-12
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    • 1970
  • This study was conducted to abserve the effects of cortisone acetate, estrogenic hormone and tocopherol on the antibody tresponse to both pullorum suspects and negatives of The increase of antibody titer was shown in a few birds after the treatment of either cortisone acetate or estrogenic hormone. There wuas no change in antibody titer of chickens treated with tocopherol.

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Tectoridin, a Poor Ligand of Estrogen Receptor α, Exerts Its Estrogenic Effects via an ERK-Dependent Pathway

  • Kang, Kyungsu;Lee, Saet Byoul;Jung, Sang Hoon;Cha, Kwang Hyun;Park, Woo Dong;Sohn, Young Chang;Nho, Chu Won
    • Molecules and Cells
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    • v.27 no.3
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    • pp.351-357
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    • 2009
  • Phytoestrogens are the natural compounds isolated from plants, which are structurally similar to animal estrogen, $17{\beta}$-estradiol. Tectoridin, a major isoflavone isolated from the rhizome of Belamcanda chinensis. Tectoridin is known as a phytoestrogen, however, the molecular mechanisms underlying its estrogenic effect are remained unclear. In this study we investigated the estrogenic signaling triggered by tectoridin as compared to a famous phytoestrogen, genistein in MCF-7 human breast cancer cells. Tectoridin scarcely binds to ER ${\alpha}$ as compared to $17{\beta}$-estradiol and genistein. Despite poor binding to ER ${\alpha}$, tectoridin induced potent estrogenic effects, namely recovery of the population of cells in the S-phase after serum starvation, transactivation of the estrogen response element, and induction of MCF-7 cell proliferation. The tectoridin-induced estrogenic effect was severely abrogated by treatment with U0126, a specific MEK1/2 inhibitor. Tectoridin promoted phosphorylation of ERK1/2, but did not affect phosphorylation of ER ${\alpha}$ at $Ser^{118}$. It also increased cellular accumulation of cAMP, a hallmark of GPR30-mediated estrogen signaling. These data imply that tectoridin exerts its estrogenic effect mainly via the GPR30 and ERK-mediated rapid nongenomic estrogen signaling pathway. This property of tectoridin sets it aside from genistein where it exerts the estrogenic effects via both an ER-dependent genomic pathway and a GPR30-dependent nongenomic pathway.