Browse > Article
http://dx.doi.org/10.5322/JES.2004.13.6.591

Study on Estrogenic Activities of Pesticide Chemicals using E-screen Assay  

Han, Sang Guk (목포해양대학교 해양시스템공학부 해양환경공학전공)
Publication Information
Journal of Environmental Science International / v.13, no.6, 2004 , pp. 591-597 More about this Journal
Abstract
In this study, sixty chemicals including 47 pesticides were screened for estrogenic activity using E-screen assay. MCF-7 cell, used in E-screen assay, is known to be proliferated by addition of estrogenic chemicals. Eight of the measured pesticides showed estrogenic activity at the concentration range of 100-10,000nM. Their relative proliferative effect (RPE) and the relative proliferative potency (RPP) were 20-65% and 0.01-1.0%, respectively, when compared with 1.0nM of $\beta-Estradiol-17-acetate(E_{2}).$ DDVP and diazinone showed most strong estrogenic potency(RPP; 1.0%) and effect(RPE; 65%) of the eight pesticides. These results are in agreement with estrogenic activity of bisphenol A is known as a positive endocrine disrupter. Also, in this study, paraquat, DDVP, 4-chloro-3-methylphenol, 2,4,6-trichlorophenol and diazinon of the measured pesticides are estimated to estrogenic chemical.
Keywords
MCF-7; E-screen assay; Pesticides; Relative proliferative effect(RPE); Relative proliferative potency (RPP); Estrogenic;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Villalobos, M., N. Olea, J. A. Brotons, M. F. Olea-Serrano, J. M. Ruiz de Almodovar and V. Pedraza, 1995, The E-screen assay: A comparison of different MCF-7 cell stocks, Environ Health Perspect, 103, 844-850.   DOI   ScienceOn
2 Han, S. K., S. Y. Han, H. J. Moon, H. S. Kim, D. H. Lee, S. H. Kim, T. S. Kim and K. L. Park, 2000, Study on estrogenic activities of phthalate esters using E-screen assay and competitive binding assay, J. Toxicol. Pub. Health, 16(2), 141-146.   과학기술학회마을
3 內海秀雄, 1998, 環境廳未來環境添創造型基礎硏究 化學物質による生物.環境負荷の總閤評價手法の開發, 環境廳, 平年9年度報告書.
4 Steinmetz, R., N. G. Brown, D. L. Allen, R. M. Gigsby and N. Ben-Jonathan, 1997, The environmental estrogen bisphenol A stimulates prolactin release in vitro and in vivo, Endocrinology, 138, 1780-1786.   DOI   ScienceOn
5 Soto, A. M., K. L. Chung and C. Sonnenschein, 1994, The pesticides endosulfan, toxaphene, and dieldrin have estrogenic effects on human estrogen-sensitive cells, Environ Health Perspect, 102, 380-383.   DOI   ScienceOn
6 Soto, A. M., C. Sonnenshein, K. L. Chung, M. F. Fernandez, N. Olea and F. O. Serrano, 1995, The E-Screen assay as a tool to identify estrogens: an update on estrogenic environmental pollutants, Environ Health Perspect, 103, 113-122.   DOI
7 Petit, F., P. L. Goff, J. P. Cravedi, Y. Valotaire and F. Pakdel, 1997, Two complementary bioassays for screening the estrogenic potency of xenobiotics: recombinant yeast for trout estrogen receptor and trout hepatocyte cultures, J. Molecular Endocrinology, 19, 321-335.   DOI   ScienceOn
8 Soto, A. M., C. Sonnenschein and T. Colborn, 1996, Special issue : Endocrine disruption and repoductive effects in wildlife and humans, Comments Toxicol., 5, 315-506.
9 Robert, J. K., P. D. George, D. Chris, F. C. Penny, K. Steve, L. George, L. Michael, J. M. Michael, M. Carol, M. Ron, M. Jack, R. Rosalind, S. Geoffrey, M. S. Daniel, S. Thomas and A. T. Hugh, 1996, Research needs for the risk assessment of health and environmental effects of endocrine disruptors: A report of the U.S. EPA-sponsored workshop, Environ Health Perspect, 104, 715-741.   DOI   ScienceOn
10 EDSTAC(EPA), 1998, Final draft report, U.S. EPA, 17-18.
11 Olea, N., R. Pulgar, P. Perez, F. Olea-Serrano, A. Rivas, A. Novillo-Fertrell, V. Pedeaza and A. M. Soto, 1996, Estrogenicity of resin-based composites and sealants used in dentistry, Environ Health Perspect, 104, 298-305.   DOI   ScienceOn