• 대한임상검사과학회지
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• 제45권1호
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• pp.37-42
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• 2013

The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of $0{\sim}5WBCs/{\mu}L$ of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of $0{\sim}5WBCs/{\mu}L$ on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0~40 cells in the 44 samples of $0WBC/{\mu}L$, and 3~95 cells in the 31 samples of $1WBC/{\mu}L$. It was observed that the nucleated cell number for cells recovered on slide was 13~100 cells in the 44 samples of $2WBCs/{\mu}L$, and more than 100 cells in the 29 samples of $3{\sim}5WBCs/{\mu}L$, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of $0{\sim}5WBCs/{\mu}L$. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as $1WBC/{\mu}L$ in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at $0WBC/{\mu}L$ by using the cell yield in a comparison between the value of $0{\sim}5WBCs/{\mu}L$ and nucleated cell number for cells recovered on slide.

• 대한수의학회지
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• 제28권2호
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• pp.285-297
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• 1988

The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328(♂ 92, ♂ 236) healthy Jindo dogs in Jindo area. The results obtained in this study were summarized as follows: 1. The mean values of red blood cell(RBC) count, hemoglobin(Hb) content and packed cell volume(PCV) in the age group of less than one year were 6.77(♂ 6.80, ♂ 6.76)${\times}10^6/{\mu}l$, 13.14 (♂ 13.53, ♂ 12.99)g/100ml and 43.28(♂ 44.47, ♂ 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(♂ 7.44, ♂ 7.42)${\times}10^6/{\mu}l$, 14.98(♂ 15.56, ♂ 14.76)g/100ml and 47.18 (♂ 48.43, ♂ 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PCV appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. 2. The mean values of mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin content(MCHC) in the age group of less than one year were 63.93(♂ 65.40, ♂ 63.30)fl, 19.41(♂ 19.90, ♂ 19.22) pg and 30.36(♂ 30.43, ♂ 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(♂ 65.09, ♂ 62.95)fl, 20. 19(♂ 20.91, ♂ 19.89) pg and 31.75(♂ 32.13, ♂ 31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. 3. The mean values of white blood cell(WBC) count in the age group of less than one year were 14,356(♂ 13,878, ♂ 14,551)/${\mu}l$, whereas the values of WBC count in the age group of one year and more were 13,394(♂ 12,656, ♂ 13,672)/${\mu}l$. The WBC count appeared to be higher in the female than in the male. No differences were found between age groups in the WBC count. 4. In WBC differential count the mean percentage of lymphocyte, monocyte, neutrophil and eosinophil of the age group less than one year were 33.45(♂ 31. 64, ♂ 34.18), 2.77(♂ 3.00, ♂ 2.68), 57.40(♂ 58.18, ♂ 57.08) and 6.38(♂ 7.18, ♂ 6.06)% respectively, while those of the age group one year and more were 30. 22(♂ 28.38, ♂ 30.90), 3.03(♂ 3.23, ♂ 2.95), 60.93(♂ 63.34, ♂ 60.03) and 5.82(♂ 5.04, ♂ 6.11)% respectively. Lymphocyte count appeared to be higher in the female and in the age group below one year than in the male and in the age group of one year and more, whereas the reverse was the case with neutrophil and monocyte counts. 5. Mean platelet count in the age group of less than one year was 377,391(♂ 398,778, ♂ 368,721)/${\mu}l$, whereas mean platelet count in the age group of one year and more was 354,657 (♂ 373,660, ♂ 347,512)/${\mu}l$. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more($305,513/{\mu}l$) than in the other age groups.

• Journal of Yeungnam Medical Science
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• 제20권1호
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• pp.79-84
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• 2003

Pyoderma gangrenosum is uncommon neutrophilic dermatosis characterized by richness of the mature neutrophilic polynuclear dermal infiltrate. Pyoderma gangrenosum is associated with variable diseases, most commonly inflammatory bowel disease, hematological diseases, malignancies, but it is reported rarely in rheumatoid arthritis. We report a case of pyoderma gangrenosum in rheumoid arthritis patient. A 50-year-old woman admitted to our hospital due to painful pretibial ulcerative skin lesions. She had been treated as rheumatoid arthritis for 8 years. At admission, body temperature was $36.5^{\circ}C$ and other vital sign was unremarkable. Physical examination revealed right pretibial ulceration, multiple pustules on left pretibial area and both palms. Laboratory studies revealed WBC count 7,600/uL (neutrophils 60.3%, eosinophil 3.2%), hemoglobin 11.4 g/dL, platelet count 319,000/uL, ESR 65 mm/hour. Other lab findings were also unremarkable. Skin biopsy was done, which showed dense dermal infiltrate of neutrophils and wound culture were negative. By 8 weeks after systemic high dose corticosteroid (1 mg/kg/day), cyclosporine A (5 mg/kg/day), sulfasalazine 2 g therapy, symptoms and skin ulceration were being improved. Without skin relapse, she is followed up our hospital with low dose corticosteroid and sulfasalazine.

• 대한수의학회지
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• 제14권1호
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• pp.9-16
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• 1974

The literature on the hematology of dairy cattle contains very important information of physiological and clinical field, but a comprehensive survey on the blood picture of Holstein cows has not been made yet in Korea. The object of the present investigation was to make good this deficiency, and to suggest standards for the blood picture of Holstein cows kept under average farming condition in this country. Observations were made on the blood picture of 30 healthy non-pregnant Holstein cows aging from 6 to 10 years. All of them were selected at random from 15 livestock farms of Deajeon area in order to determine the hematological values from January to Feburary, 1974. The ranges and mean values of erythrocyte count, hemoglobin in blood, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and total and differential leukocyte count were determined. The result obtained in this work were summerized as follows: 1. Ranges and mean values(with standard error) of erythrocyte count, hemoglobin in blood and hematocrit values were 4.90~7.82 and $5.83{\pm}0.12{\times}10^6/mm^3$, 7.8~10.3 and $8.7{\pm}0.11g/100ml$, and 26~38 and $30.2{\pm}0.53$ mI/100 ml, respectively. 2. Mean corpuscular hemoglobin, mean corpuscular volume, and mean corpuscular hemoglobin concentration showed ranges and mean values(with standard error) of 13.2~17.4 and $14.8{\pm}0.22$ pg, 46.0~71.8 and $52.3{\pm}0.31{\mu}m^3$, and 21.6~32.7 and $28.6{\pm}0.47$ g/100 ml, respectively. 3. Total leukocyte count showed a range of 6,300~13,600 and a mean value(with standard error) of $10,035{\pm}346/mm^3$. 4. Ranges and mean values(with standard error) of differential leukocyte counts of total neutrophil were 21~40 and $36.4{\pm}0.7%$, 1,638~4,080 and $3,233{\pm}111/mm^3$, of band neutrophil 0~9 and $3.9{\pm}0.5%$, 0~1,028 and $390{\pm}54/mm^3$, of segmented neutrophil 18~35 and $28.4{\pm}0.7%$, 1,386~3,672 and $2,842{\pm}103/mm^3$, of lymphocyte 46~69 and $55.3{\pm}1.1%$, 3,380~8,976 and $5,535{\pm}263/mm^3$, of monocyte 0~4 and $1.7{\pm}0.2%$, 0~324 and $116{\pm}21/mm^3$, of eosinophil 4~15 and $10.8{\pm}0.6%$, 540~1,725 and $1,082{\pm}72/mm^3$. No basophil was observed in this work.

• Journal of Animal Science and Technology
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• 제51권4호
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• pp.321-328
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• 2009

본 연구는 초유 급여를 마친 갓 태어난 Holstein 송아지 14두를 대조구(Con) 및 시험구(IgY)로 나누어 49일 동안 대용유를 급여하면서 난황항체(IgY구)를 1주일 동안 투여하여 사양성적, 분변 상태 및 미생물 균총, 혈액내 생화학적 성분 및 면역관련 지표인 백혈구 성상을 조사하여 가장 바람직한 송아지 포유 및 육성 방법을 조사하였다. 시험결과로서 포유 송아지에게 IgY 투여시 체중 및 증체는 대조구(Con) 및 시험구(IgY)간에 유의적 차이 없이 모두 비슷한 성적을 보였다. 송아지 분변의 대장균(E. coli) 및 유산균 총균수를 조사한 결과 IgY 투여에 따른 대장균 및 유산균 수의 차이가 나타나지 않았으나, 분의 상태는 시험구에서 7주째 유의적으로(P<0.05) 고형상태로 배설되었다. 송아지 혈액의 백혈구 감별계수(%)를 조사한 호중구, 림프구, 단핵구, 호염기구 등의 상대적 %는 모든 구에서 정상적인 백혈구 감별 계수를 보여 IgY 투여에 따른 면역 반응에는 차이가 없는 것으로 관찰되었다. 또한 혈액내 존재하는 총 단백질, albumin 및 IgG 등에서도 대조구 및 시험구 모두 비슷한 수준을 나타내었다. 결론적으로 포유 송아지에서 1주 동안 IgY 투여는 증체, 면역작용 및 분변 미생물의 균총에는 특이적 영향이 없는 것으로 사료되어, 송아지에게 생후 24시간 이내 초유를 정확하게 급여하고 사양관리에 충실 할 경우 송아지의 포유 및 육성은 성공적으로 이루어 질 수 있을 것으로 사료된다.

• 대한임상검사과학회지
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• 제37권1호
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• pp.16-21
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• 2005

To find out the effectiveness of clinical examination for detection of dermatophagoides farinae allergen asthma disease, 50 patients (control group) and 50 healthy persons matched by sex and age to the control group, were tested for allergen asthma. The results of the study follow. The ESR there was significantly different from the comparisons of the patients' normal reference values, 95.3 % in ESR, patient groups allergen asthma and heamatology values with control group. The ESR level of allergen asthma patients, $62.23{\pm}35.09$ mm/hr, was higher than that of the controls, $9.47{\pm}5.36$ mm/hr (p<0.001). The eosinophil count level of the patients, $370.65{\pm}365.45mm^3$, was higher than that of the controls with $171.99{\pm}131.80mm^3$ (p<0.001). The IgE level of the patients, $1137.79{\pm}784.69IU/ml$, was higher than that of the controls with $71.29{\pm}14.28 IU/ml$ (p<0.001).

• 대한수의학회지
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• 제29권2호
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• pp.129-134
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• 1989

Fecal samples were taken from 402 cows in Posung, Chonnam where was designated as a place for Korean native cattle breeding. Prevalence of internal parasitisms were determined by the fecal examinations using the floatation and sedimentation procedures. 62.9% of the cows were found as positive cases with excretion of the eggs of Fasciola hepatica in the fecal specimens. Of those infected with F hepatica 97 cows free of other pathogenic intestinal parasites were chosen for albendazole treatment. Albendazole tablets(10mg/kg) were administered to the cows twice at the interval of 4 weeks. Blood samples were collected via jugular vein prior to the first treatment, four weeks after the first treatment and four weeks after the second treatment, respectively. At the same time fecal samples were collected for parasitological examinations by sedimentation methods. The mean pretreatment count was 44 fluke eggs per gram of feces, which compared with 27 epg and 17 epg four weeks after the first and second treatment, respectively. Most of the hematological and biochemical values fluctuated within the normal ranges during the experiment. Eosinophil counts were high initially, decreased after the first treatment and thereafter remained steady. The opposite was the case with aspartate and alanine aminotransferases.

• Childhood Kidney Diseases
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• 제19권2호
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• pp.167-170
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• 2015

Hypereosinophilic syndrome (HES) is characterized by the presense of hypereosinophilia with evidence of target organ damage. We report a patient diagnosed with eosinophilic cystitis and HES. A 7 year old boy had hematuria, dysuria, and increased urinary frequency for 1 day. Laboratory examinations revealed hypereosinophilia (eosinophils, $2,058/{\mu}L$), hematuria, and proteinuria. Abdominal sonography revealed diffuse and severe wall thickening of the bladder. The patient was treated initially with antibiotics. However, his symptoms did not improve after 7 days. A computed tomography scan demonstrated severe wall thickening of the bladder and the hypereosinophilia persisted (eosinophils, $2,985/{\mu}L$). The patient complained of chest discomfort, dyspnea, epigastric pain, and vomiting on hospital day 10. Parasitic, allergic, malignancy, rheumatologic, and immune workups revealed no abnormal findings. Chest X-rays, electrocardiography, and a pulmonary function test were normal; however, the hypereosinophilia was aggravated (eosinophils, $3,934/{\mu}L$). Oral deflazacort was administered. A cystoscopic biopsy showed chronic inflammation with eosinophilic infiltration. The patient's respiratory, gastrointestinal, and urinary symptoms improved after 6 days of steroids, and he was discharged. The eosinophil count decreased dramatically ($182/{\mu}L$). The hypereosinophilia waxed and waned for 7 months, and the oral steroids were tapered and stopped. This case describes a patient diagnosed with eosinophilic cystitis and HES.

• Parasites, Hosts and Diseases
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• 제51권4호
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• pp.467-470
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• 2013

The present study reports a human case of cutaneous gnathostomiasis with recurrent migratory nodule and persistent eosinophilia in China. A 52-year-old woman from Henan Province, central China, presented with recurrent migratory reddish swelling and subcutaneous nodule in the left upper arm and on the back for 3 months. Blood examination showed eosinophila (21.2%), and anti-sparganum antibodies were positive. Skin biopsy of the lesion and histopathological examinations revealed dermal infiltrates of eosinophils but did not show any parasites. Thus, the patient was first diagnosed as sparganosis; however, new migratory swellings occurred after treatment with praziquantel for 3 days. On further inquiring, she recalled having eaten undercooked eels and specific antibodies to the larvae of Gnathostoma spinigerum were detected. The patient was definitely diagnosed as cutaneous gnathostomiasis caused by Gnathostoma sp. and treated with albendazole (1,000 mg/day) for 15 days, and the subsequent papule and blister developed after the treatment. After 1 month, laboratory findings indicated a reduced eosinophil count (3.3%). At her final follow-up 18 months later, the patient had no further symptoms and anti-Gnathostoma antibodies became negative. Conclusively, the present study is the first report on a human case of cutaneous gnathostomiasis in Henan Province, China, based on the past history (eating undercooked eels), clinical manifestations (migratory subcutaneous nodule and persistent eosinophilia), and a serological finding (positive for specific anti-Gnathostoma antibodies).

• 대한한방소아과학회지
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• 제15권2호
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• pp.177-188
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• 2001

Background : Atopic Dermatitis is thought to be a common and chronic relapsing inflammatory skin disease that probably results from allergic reaction. Because it make some serious problems in children, it is needed to treat and at least subside symptoms. Objective : The aim of this study was to evaluate the efficacy of GamiYeoldahansotang(加味熱多寒少湯) for treating Atopic Dermatitis and survey the general characteristics in children with Atopic Dermatitis. Method : Atopic Dermatitis with typical clinical symptoms were included in this study. Forty two patients were treated with hot water extract of GamiYeoldahansotang(加味熱多寒少湯) for four weeks. Clinical evaluation were made by Jacob T scoring system before and after treatment. Total Serum IgE, Eosinophil count were also conducted. Results : Reduction in body surface area was not observed. But significant reductions of severity scores before and after adminstration of GamiYeoldahansotang(加味熱多寒少湯) were observed in all of three groups: mind, moderate, severe. specially severe intensity group was very statistically significant.(p<0.05) Conclusion : We speculate that GamiYeoldahansotang(加味熱多寒少湯) has some therapeutic effects in mitigating the symptoms of Atopic Dermatitis.