• Title/Summary/Keyword: Enzymatic antioxidants

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Antioxidant Activity of Aqueous Extract of Coscinium fenestratum in STZ-Nicotinamide Induced Diabetic Rats

  • Punitha, I.S.R.;Bhat, Nalini;Rajendran, K.;Shirwaikar, Arun;Shirwaikar, Annie
    • Natural Product Sciences
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    • v.11 no.3
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    • pp.155-159
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    • 2005
  • The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.

Residue by elapsed time of non-enzymatic antioxidants in dentifrice (세치제에 함유된 비효소계 항산화제의 경시변화에 따른 잔류량)

  • Park, Jung-Eun;Park, Yong-Duk;Hong, Tae-Gi;Jang, Jong-Hwa
    • Journal of Korean society of Dental Hygiene
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    • v.16 no.5
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    • pp.783-790
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    • 2016
  • Objectives: The purpose of this study is to evaluate the non-enzymatic antioxidants stabilities in dentifrices by ascorbic acid and tocopherol according to the chemical condition. Methods: For the analysis of two antioxidants, HPLC UV detector system was used. HPLC was performed using sodium sulfate, acetonitrile(ACN), methanol(MeOH) and measuring absorbance at 240-295 nm. To confirm general pH reaction of two compounds, buffer solution was prepared for the analysis. The dentifrice was titrated by pH so as to examine the change of elapsed time in dentifrice. Linearity of calibration curve of two antioxidants was measured. Results: Each compound showed good linearity at optimized wavelength as well as showing good precision. General pH reaction of two antioxidants was examined. Ascorbic acid showed the highest residue(63.23%) at pH 10 and the lowest residue(2.77%) at pH 4. Tocopherol showed the highest residue(55.70%) at pH 7 and the lowest residue(3.31%) at pH 4. As a result of changing elapsed time of antioxidants in dentifrice by pH, components were remained stably at low temperature($39.2^{\circ}F$) and pH 7. Conclusions: It is necessary to keep dentifrice including ascorbic acid and tocopherol, and non-enzymatic antioxidants at pH 7 and low temperature for improving chemical stability.

Assessment of Salinity-Induced Antioxidative Defense System of Diazotrophic Cyanobacterium Nostoc muscorum

  • Srivastava, Ashish Kumar
    • Journal of Microbiology and Biotechnology
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    • v.20 no.11
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    • pp.1506-1512
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    • 2010
  • The present study examined the salinity-induced oxidative damage and differential response of enzymatic and nonenzymatic antioxidants of Nostoc muscorum. As compared with carotenoid content that showed induction, the chlorophyll and phycocyanin contents were inhibited after salt stress. Acceleration of lipid peroxidation and peroxide production suggested the onset of oxidative damage. The activities of all studied enzymatic antioxidants were significantly increased by salt stress, with maximum induction occuring with superoxide dismutase (154.8% at 200 mM NaCl treatment). Interestingly, under severe stress condition (250 mM NaCl), ascorbate peroxidase seemed to be more crucial than catalase for peroxide scavenging. Among the studied nonenzymatic antioxidants, ${\alpha}$-tocopherol was induced maximally (56.0%); however, ascorbate and reduced glutathione were increased by only 8.9% after 250 mM NaCl treatment as compared with control cells. Therefore, salinity was found to induce the antioxidative defense system of N. muscorum.

Micronutrients and superoxide dismutase in postmenopausal women with chronic periodontitis: a pilot interventional study

  • Daiya, Sunita;Sharma, Rajinder Kumar;Tewari, Shikha;Narula, Satish Chander;Sehgal, Paramjeet Kumar
    • Journal of Periodontal and Implant Science
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    • v.44 no.4
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    • pp.207-213
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    • 2014
  • Purpose: The study was aimed at investigating changes in periodontal parameters and superoxide dismutase activity triggered by root surface debridement with and without micronutrient supplementation in postmenopausal women. Methods: Forty-three postmenopausal chronic periodontitis patients were divided into two groups: group 1 (n=22) were provided periodontal treatment in the form of scaling and root planing (SRP) and group 2 (n=21) patients received SRP along with systemic administration of micronutrient antioxidants. Patients in both groups were subjected to root surface debridement. Group 2 patients also received adjunctive micronutrient antioxidant supplementation. Serum and salivary superoxide dismutase (SOD) activity along with periodontal parameters were recorded at baseline and 3 months after therapy. Results: Salivary and serum SOD values significantly (P<0.05) improved with periodontal treatment. Improvement in systemic enzymatic antioxidant status along with reduction in gingival inflammation and bleeding on probing (%) sites was significantly greater in group 2 as compared to group 1. Conclusions: Adjunctive micronutrient supplements reduce periodontal inflammation and improve the status of systemic enzymatic antioxidants in postmenopausal women.

Hepatoprotective effect of kasni against aflatoxin B1 induced hepatic damage in mice

  • Naaz Farah;Abdin MZ;Javed Saleem
    • Advances in Traditional Medicine
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    • v.6 no.3
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    • pp.196-201
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    • 2006
  • The efficacy of alcoholic extract of Kasni (Cichorium intybus L.) to control hepatic damage induced by aflatoxin $B_1$ was explored in Swiss albino mice. Aflatoxin $B_1$ was administered orally to the mice with a daily dose of $66.6{\mu}g/kg$ body weight till three months. A signifi-cant increased in thio barbituric acid reactive substances (TBARS) levels with concomitant reduction in enzymatic (glutathione-s-transferase, glutathione peroxidase, superoxide dismutase, and catalase) and nonenzymatic (reduced glutathione) antioxidants were shown in aflatoxin treated group of mice. However, there was a significant reduction in increased TBARS levels and elevation in enzymatic. and non enzymatic antioxidant levels in group of mice which received alcoholic extract of kasni (300 mg/kg bw / day) along with aflatoxin. Histopathological analysis of liver samples also confirmed the hepatoprotective effect of kasni extract. These results suggest that kasni shows hepatoprotective effect against aflatoxin $B_1$ induced hepatic damage in mice.

Antioxidant Effect of Tropical Seaweed Pylaiella littoralis Extracts Collected from Chuuk Lagoon in Federated States of Micronesia (마이크로네시아에 서식하는 해조류 Pylaiella littoralis 추출물의 항산화 효과)

  • Ye, Bo-Ram;Jang, Ji-Yi;Kwon, Young-Kyung;Jeon, Seon-Mi;Jeong, Joo-Yeong;Kang, Do-Hyung;Oh, Chul-Hong;Kim, Ji-Hyung;Affan, Abu;Hyun, Jung-Ho;Heo, Soo-Jin
    • Ocean and Polar Research
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    • v.34 no.3
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    • pp.297-304
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    • 2012
  • Pylaiella littoralis was collected in the Chuuk lagoon of the Federated States of Micronesia (FSM). The FSM has a variety of coral reef ecosystems, which provide essential materials, such as minerals, vitamins, essential amino acids, for marine organisms. In this study, the antioxidant activities of ethanol and enzymatic extracts of P. littoralis were evaluated by measuring their scavenging activities on DPPH free radical, Alkyl radical, hydroxyl radical and cell viability. The enzymatic extracts were hydrolyzed to prepare water soluble extracts by using five carbohydrate degrading enzymes (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and five proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase, and Protamex). As a result, the enzymatic extracts prepared by Flavourzyme, Ultraflo, and Kojizyme exhibited the greatest effects in DPPH free radical, alkyl radical scavenging activity and cell viability. Also, these enzymatic extracts had a higher antioxidant effect then commercial antioxidants in DPPH free radical and Alkyl radical scavenging activity. This study suggests that P. littoralis might be a useful source of natural antioxidants for the development of dietary supplements.

Free Radical Scavenging Activity of Enzymatic Hydrolyzates of Hot Water Extract from the Shell of Reeve's Turtle (Chinemys reevesii)

  • Je, Jae-Young;Kim, Eun-Kyung;Park, Pyo-Jam;Kang, Mi-Kyung;Ahn, Chang-Bum
    • Fisheries and Aquatic Sciences
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    • v.11 no.2
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    • pp.71-75
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    • 2008
  • The shell of Reeve's turtle has been used as a traditional folk medicine in Korea. We produced a hot water extract from Reeve's turtle shell according to the traditional medical practice. To release bioactive peptides, the hot water extract was enzymatically hydrolyzed with various proteases, and the free radical scavenging activity of the hydrolysate was investigated against 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl and peroxyl radicals. The free radical scavenging activity of the enzymatic hydrolysates varied from 1 to 79% depending on the enzymes, free radical species, and concentration. The $EC_{50}$ values demonstrated that the enzymatic hydrolysates of hot water extract from the shell of Reeve's turtle are potential antioxidants.

Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis

  • Lee, Seung-Hong;Kim, Areum-Daseul;Kang, Min-Cheol;Lee, Joon-Baek;Jeon, You-Jin
    • ALGAE
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    • v.24 no.3
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    • pp.169-177
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    • 2009
  • In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.

Potential Antioxidant Activites of Enzymatic Digests from Benthic Diatoms Achnanthes longipes, Amphora coffeaeformis, and Navicula sp. (Bacillariophyceae)

  • Lee, Seung-Hong;Karawita, Rohan;Affan, Abu;Lee, Joon-Baek;Lee, Bae-Jin;Jeon, You-Jin
    • Preventive Nutrition and Food Science
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    • v.13 no.3
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    • pp.166-175
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    • 2008
  • In this study, we focused on natural water-soluble antioxidants from the Jeju benthic diatoms, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) and their potential antioxidant activity was assessed. Among the enzymatic digests, Neutrase digest from A. coffeaeformis exhibited the highest effect in DPPH radical scavenging. Flavourzyme (48.7%), Viscozyme (47.4%) and Celluclast (45.7%) digests from Navicula sp. exhibited higher $O^{{\cdot}-}_2$ radical scavenging activity. Viscozyme digest from A. coffeaeformis (45.9%) possessed the highest effects in hydroxyl radical scavenging. Termamyl (89.3%) and Protamex (88.8%) digests from A. coffeaeformis had strong metal chelating activity. Lipid peroxidation was significantly inhibited in Termamyl and Kojizyme digests from A. longipes, AMG and Termamyl digests from Navicula sp. and Kojizyme digest from A. coffeaeformisi. These data suggest that enzymatic digests of the Jeju benthic diatoms might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.

Chemopreventive Potential of Coumarin in 7,12-dimethylbenz[a] anthracene Induced Hamster Buccal Pouch Carcinogenesis

  • Baskaran, Nagarethinam;Manoharan, Shanmugam;Karthikeyan, Sekar;Prabhakar, Murugaraj Manoj
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.5273-5279
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    • 2012
  • The aim of the present study was to investigate the chemopreventive effect of coumarin against 7, 12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis by monitoring tumor incidence and histopathological changes as well as by analyzing the status of biochemical markers (lipid peroxidation, enzymatic and non-enzymatic antioxidants, phase I and phase II detoxification enzymes). Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We noted 100% tumor formation with marked abnormalities in the biomarkers status in hamsters treated with DMBA alone. Oral administration of coumarin at a dose of 100 mg/kg body weight (bw) to DMBA treated hamsters completely prevented the tumor formation as well as restored the staus of biochemical variables. The results of the present study thus suggest that the chemopreventive effect of coumarin is probably due to its anti-lipid peroxidative potential and modulating effect on carcinogen detoxification agents in favor of the excretion of ultimate carcinogenic metabolites of DMBA during DMBA-induced hamster buccal pouch carcinogenesis.