Browse > Article
http://dx.doi.org/10.4217/OPR.2012.34.3.297

Antioxidant Effect of Tropical Seaweed Pylaiella littoralis Extracts Collected from Chuuk Lagoon in Federated States of Micronesia  

Ye, Bo-Ram (Global Bioresources Research Center, KIOST)
Jang, Ji-Yi (Global Bioresources Research Center, KIOST)
Kwon, Young-Kyung (Global Bioresources Research Center, KIOST)
Jeon, Seon-Mi (Global Bioresources Research Center, KIOST)
Jeong, Joo-Yeong (Global Bioresources Research Center, KIOST)
Kang, Do-Hyung (Global Bioresources Research Center, KIOST)
Oh, Chul-Hong (Global Bioresources Research Center, KIOST)
Kim, Ji-Hyung (Global Bioresources Research Center, KIOST)
Affan, Abu (Global Bioresources Research Center, KIOST)
Hyun, Jung-Ho (Department of Environmental Marine Sciences, College of Science and Technology Hanyang University)
Heo, Soo-Jin (Global Bioresources Research Center, KIOST)
Publication Information
Ocean and Polar Research / v.34, no.3, 2012 , pp. 297-304 More about this Journal
Abstract
Pylaiella littoralis was collected in the Chuuk lagoon of the Federated States of Micronesia (FSM). The FSM has a variety of coral reef ecosystems, which provide essential materials, such as minerals, vitamins, essential amino acids, for marine organisms. In this study, the antioxidant activities of ethanol and enzymatic extracts of P. littoralis were evaluated by measuring their scavenging activities on DPPH free radical, Alkyl radical, hydroxyl radical and cell viability. The enzymatic extracts were hydrolyzed to prepare water soluble extracts by using five carbohydrate degrading enzymes (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and five proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase, and Protamex). As a result, the enzymatic extracts prepared by Flavourzyme, Ultraflo, and Kojizyme exhibited the greatest effects in DPPH free radical, alkyl radical scavenging activity and cell viability. Also, these enzymatic extracts had a higher antioxidant effect then commercial antioxidants in DPPH free radical and Alkyl radical scavenging activity. This study suggests that P. littoralis might be a useful source of natural antioxidants for the development of dietary supplements.
Keywords
Federated States of Micronesia; Pylaiella littoralis; antioxidant activity; enzymatic extract; radical scavenging activity;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Huang X, Dai J, Fournier J, Ali AM, Zhang Q, Frenkel K (2002) Ferrous ion autoxidation and its chelation in ironloaded human liver HepG2 cells. Free Radical Bio Med 32(1):84-92   DOI   ScienceOn
2 Johannes RE, Coles SL, Kuenzel NT (1970) The role of zooplankton in the nutrition of some scleractinian corals. Limnol Oceanogr 15(4):579-586   DOI
3 Kang SM, Kim KN, Lee SH, Anh G, Cha SH, Kim AD, Yang XD, Kang MC, Jeon YJ (2011) Anti-inflammatory activity of polysaccharide purified from AMG-assistant extract of Ecklonia cava in LPS-stimulated RAW 264.7 macrophages. Carbohyd Polym 85(1):80-85   DOI   ScienceOn
4 Kim YA, Kong CS, Um YR, Lee JI, Nam TJ, Seo Y (2008) Antioxidant Efficacy of Extracts from a Variety of Seaweeds in a Cellular System. Ocean Science J 43(1):31-37   DOI
5 Koppenol WH, Liebman JF (1984) The oxidizing nature of the hydroxyl radical. A comparison with the ferryl ion ($FeO^{2+}$). J Phys Chem 88(1):99-101   DOI
6 Koyanagi S, Tanigawa N, Nakagawa H, Soeda S, Shimeno H (2003) Oversulfation of fucoidan enhances its antiangiogenic and antitumor activities. Biochem Pharmacol 65(2):173-179   DOI   ScienceOn
7 Kuda T, Tsunekawa M, Goto H, Araki Y (2005) Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. J Food Compos Anal 18(7):625-633   DOI   ScienceOn
8 Lamela M, Anca J, Villar R, Otero J, Calleja JM (1989) Hypoglycemic activity op several seaweed extracts. J Ethnopharmacol 27(1-2):35-43   DOI   ScienceOn
9 Shahidi F, Naczk M (2004) Antioxidant properties of food phenolics. In : Shahidi F, Naczk M (eds) Phenolics in food and nutraceuticals. FL:CRC Press, Boca Raton, pp 403-437
10 Shetty K, Curtis OF, Levin RE, Witkowsky R, Ang W (1995) Prevention of vitrification associated with in vitro shoot culture of oregano. (Origanum vulgare) by Pseudomonas spp. J Plant Physiol 147(3-4):447-451   DOI   ScienceOn
11 Synytsya A, Kim WJ, Kim SM, Pohl R, Syntsya A, Kvasni ka F, opikova J, Park YIl (2010) Structure and antitumor activity of fucoidan isolated from sporophyll of Korean Brown seaweed Undaria pinnatifida. Carbohyd Polym 81(1):41-48   DOI   ScienceOn
12 Xing R, Yu H, Liu S, Zhang W, Zhang Q, Li Z, Li P (2005) Antioxidant activity of differently regioselective chitosan sulfates in vitro. Bioorgan Med Chem 13(4): 1387-1392   DOI   ScienceOn
13 Siriwardhana N, Kim KN, Lee KW, Kim SH, Ha JH, Song CB, et al (2008) Optimisation of hydrophilic antioxidant extraction from Hizikia fusiformis by integrating treatments of enzymes, heat and pH control. Int J Food Sci Tech 43(4):589-596
14 Wiseman H (1996) Dietary influences on membrane function; important in protection against oxidative damage and disease. J Nutr Biochem 7(1):2-15   DOI   ScienceOn
15 Witvrouw M, Clercq ED (1997) Sulfated Polysaccharides Extracted from Sea Algae as Potential Antiviral Drugs. Gen Pharmacol 29(4):497-511   DOI   ScienceOn
16 Lee JH, Park JC, Choi JS (1996) The Antioxidant Activity of Ecklonia stolonifera. Arch Pharm Res 19(3):223-227   DOI
17 Li Y, Qian ZJ, Ryu BM, Lee SH, Kim MM, Kim SK (2009) Chemical components and its antioxidant properties in vitro: An edible marine brown alga, Ecklonia cava. Bioorgan Med Chem 17(5):1963-1973   DOI   ScienceOn
18 Lim SN, Cheung PCK, Ooi VEC, Ang PO (2002) Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J Agr Food Chem 50(13):3862- 3866   DOI   ScienceOn
19 Matsukawa R, Dubinsky Z, Kishimoto E, Masaki K, Masuda Y, Takeuchi T, Chihara M, Yamamoto Y, Niki E, Karube I (1997) A comparison of screening methods for antioxidant activity in seaweeds. J App Phys 9(1):29-35
20 Mabeau S, Fleurence J (1993) Seaweed in food products: Biochemical and nutritional aspects. Trends Food Sci Tech 4(4):103-107   DOI   ScienceOn
21 Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y (1996) Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Bio Med 21(6):895-902   DOI   ScienceOn
22 Oki T, Masuda M, Furuta S, Nishibia Y, Terahara N, Suda I (2002) Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars. J Food Sci 67(5):1752-1756   DOI   ScienceOn
23 Patel RP, Cornwell T, Darley-USMAR, VM (1999) The biochemistry of nitric oxide and peroxynitrite : Implications for mitochondrial function. In: Cadenas E, Packer L (ed) Understanding the Process of Ageing: The roles of mitochondria, free radicals, and antioxidants. Academic Press, New York, pp 39-40
24 Reaven PD, Witzum JL (1996) Oxidised LDL in atherogenesis: Role of dietary modification. Annu Rev Nutr 16(1):51- 71   DOI   ScienceOn
25 Rosen GM, Rauckman EJ (1984) Spin trapping of superoxide and hydroxyl radicals. In: Packer L (ed) Methods in enzymology. Academic Press, New York, pp 198-209
26 Heo SJ, Park EJ, Lee KW, Jeon YJ (2005) Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresource Technol 96(14):1613-1623   DOI   ScienceOn
27 Hiramoto K, Johkoh H, Sako K, Kikugawa K (1993) DNA breaking activity of the carbon-centered radical generated from 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH). Free Radical Res 19(5):323-332   DOI
28 AOAC. (1990) Official Method of Analysis of the Association of Official Analytical Chemists (15th eds), Washington D.C
29 권문상, 노재훈, 이미진 (2005) 남태평양 해양의 잠재력과 우 리나라의 새로운 연구개발 투자 분야에 대한 고찰. Ocean and Polar Res 27(4):419-431   DOI   ScienceOn
30 하태열, 강성호, 권태연, 안지윤, 김성란, 김동수 (2006) 극지 미세조류의 유용성분 함량 및 항산화 활성. Ocean and Polar Res 28(1):37-43   DOI   ScienceOn
31 Aruoma OI (1999) Antioxidant action of plant foods: Use of oxidative DNA damage, as a tool for studying antioxidant efficacy. Free Radical Res 30(6):419-427   DOI   ScienceOn
32 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47(4):936-941
33 Duan XJ, Zhang WW, Li XM, Wang BG (2006) Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem 95(1):37-43   DOI   ScienceOn
34 Grice HC (1988) Safety evaluation of butylated hydroxyanisole from the perspective of effects on forestomach and oesophageal squamous epithelium. Food Chem Toxicol 26(8):717-723   DOI   ScienceOn
35 Halliwell B, Gutteridge JM (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219(1): 1-14
36 Haung HL, Wang BG (2004) Antioxidant capacity and lipophilic content of seaweeds collected from the Qingdao coastline. J Agr Food Chem 52(16):4993-4997   DOI   ScienceOn
37 Heidelberg KB, Sebens KP, Purcell JE (2004) Composition and sources of near reef zooplankton on a Jamaican forereef along with implications for coral feeding. Coral Reefs 23(2):263-276