Browse > Article
http://dx.doi.org/10.4490/ALGAE.2009.24.3.169

Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis  

Lee, Seung-Hong (Department of Marine life Science, Jeju National University)
Kim, Areum-Daseul (Department of Marine life Science, Jeju National University)
Kang, Min-Cheol (Department of Marine life Science, Jeju National University)
Lee, Joon-Baek (Department of Earth and Marine Sciences, Jeju National University)
Jeon, You-Jin (Department of Marine life Science,Jeju National University,Marine and Environmental Research Institute,Jeju National University)
Publication Information
ALGAE / v.24, no.3, 2009 , pp. 169-177 More about this Journal
Abstract
In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.
Keywords
antioxidant activity; Dactylococcopsis fascicularis; enzymatic digests; Pediastrum duplex;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Brown M.R. 1991. The amino-acid and sugar composition of 16 species of microalgae used in mariculture. J. Exp. Mar. Biol. Ecol. 145: 79-99   DOI   ScienceOn
2 Buyukuroglu M.E., Gulcin I, Oktay M. and Kufrevioglu O.I. 2001. In vitro antioxidant properties of dantrolene sodium. Pharmacol. Res. 44: 491-495   DOI   ScienceOn
3 Decker E.A. And Welch B. 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. J. Agric Food Chem. 38: 674-677   DOI
4 Ramos E.A.P. and Xiong Y.L. 2002. Antioxidant Activity of Soy Protein Hydrolysates in a Liposomal System. J. Food Sci. 67:2952-2956   DOI   ScienceOn
5 Brown M.R. and Jeffrey S.W., 1992. Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 1. Amino acids, sugars and pigments. J. Exp. Mar. Biol. Ecol. 161: 91-113   DOI   ScienceOn
6 Lindenschmidt R.C., Trika A.F., Guard M.E. and Witschi H.P. 1986. The effect of dietary butylated hydroxy toluene on liver and colon tumor development in mice. Toxicol. 38:151-160   DOI   ScienceOn
7 Radi R., Beckman J.S., Bush K.M. and Freeman B.A. 1991. Peroxynitrite induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch. Biochem. Biophys. 288: 481-487   DOI   ScienceOn
8 Ruperez P., Ahrazem O. and Leal J.A. 2002. Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J. Agric. Food Chem. 50: 840-845   DOI   ScienceOn
9 Fridovich I. 1995. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64: 97-112   DOI   ScienceOn
10 Cox D.A. and Cohen M.L. 1996. Effects of oxidized low density lipoproteins on vascular contraction and relaxation. Pharmacol. Rev. 48: 3-9
11 Fuentes M.M.R., Fernandez G.G.A., Perez J.A.S. and Guerrero J.L.G. 2000. Biomass nutrient profiles of the microalga Porphyridium cruentum. Food Chem. 70: 345-353   DOI   ScienceOn
12 Garrat D.C. 1964. The Quantitative Analysis of Drugs. Chapman and Hall, Tokyo, Japan, pp. 456-458
13 Siriwardhana N., Jeon Y.J., Kim S.H., Ha J.H., Heo S.J. and Lee K.W. 2004. Enzymatic Hydrolysis for Effective Extraction of Antioxidative Compounds from Hizikia fusiformis. Algae 19: 59-68   DOI   ScienceOn
14 Liu F. and Ng T.B. 2000. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci. 66: 725-735   DOI   ScienceOn
15 Lu F. and Foo L.Y. 1995. Toxicological aspects of food antioxidants. In: Madhavi D.L., Deshpande S.S. and Salunkhe D.K. (eds), Food antioxidants. Marcel Dekker, New York. pp. 73-146
16 Lu Y. And Foo L.Y. 2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68: 81-85   DOI   ScienceOn
17 Moncada S., Palmer R.M. and Higgs E.A. 1991. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109-142
18 Karawita R., Senevirathne M., Athukorala Y., Affan A., Lee Y.J., Kim S.K., Lee J.B. and Jeon Y.J. 2007. Protective effect of enzymatic extracts from microalgae against DNA damage induced by $H_{2}O_{2}$. Mar Biotech. 9: 479-490   DOI   ScienceOn
19 Campo J.A.D., Moreno J., Rodriguez H., Vargas M.A., Rivas J. and Guerrero M.G. 2000. Carotenoid content of chlorophycean microalgae: factors determing lutin accumulation in Muriellopsis sp. (Chlorophata). J. Biotechnol. 76: 51-59   DOI   ScienceOn
20 Chandler S.F. and Dodds J.H. 1993. The effect of phosphate, nitrogen, and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rep. 2: 105-110   DOI   ScienceOn
21 Chu F.E., Dupuy J.L. and Webb K.L. 1982. Polysaccharide composition of five algal species used as food for larvae of the American oyster, Crassostrea virginica. Aquaculture 29: 241-252   DOI   ScienceOn
22 Siriwardhana N., Lee K.W., Kim S.H., Ha J.W. and Jeon Y.J. 2003. Antioxidant activiy of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci. Tech. Int. 9: 339-346   DOI   ScienceOn
23 Spitz T.T., Bergman M., Moppes D., Grossman S. and Arad M.S. 2005. Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J. App. Phycol. 17: 215-222   DOI   ScienceOn
24 Gulcin I., Oktay M., Kufrevioglu O.I and Aslan, A. 2002. Determination of antioxidant activity of lichen Cetraria islandica (L). Ach. J. Ethnopharmacol. 79: 325-329   DOI   ScienceOn
25 Halliweill B. 1991. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. Ame. J. Med. 91: 14-19   DOI   ScienceOn
26 Hirata T., Tanaka M., Ooike M., Tsunomura T. and Sakaguchi M. 2000. Antioxidant activities of phycocyanobilin prepared from Spirulina platensis. J. Appl. Phycol. 12: 435-439   DOI   ScienceOn
27 Sherwin E.R. 1990. Antioxidants. In: Branen A. I., Davidson P. M. and Salminen S. (eds), Food Additives. Marcel Dekker, New York, pp. 139-292
28 Chung S.K., Osawa T. and Kawakishi S. 1997. Hydroxyl radicalscavenging effects of spices and scavengers from black mustard (Brassica nigra). Biosci. Biotechnol. Biochem. 6: 118-123
29 Gulcin I., Beydemir S., Ahmet H.A., Elmasta M. and Buyukokuroglu M.E. 2004. In vitro antioxidant properties of morphine. Pharmacol. Res. 49: 59-66   DOI   ScienceOn
30 Shahidi F. and Wanasundara P.K.J.P.D. 1992. Phenolic antioxidants. Crit. Rev. Food Sci. Nutr. 32: 67-103   DOI   ScienceOn
31 Muller H.E.1995. Detection of hydrogen peroxide produced by Microorganism on ABTS-peroxidase medium. Zentralbl. Bakteriol. Mikrobio. Hyg. 259: 151-158
32 Lee S.H., Karawita R., Affan A., Lee J.B., Lee B.J. and Jeon Y.J. 2008. Potential antioxidant activites of enzymatic digests from benthic diatoms Achnanthes longipes, Amphora coffeaeformis, and Navicula sp. (Bacillariophyceae). J. Food Sci. Nutr. 13: 166-175   DOI   ScienceOn
33 Liang S., Liu X., Chen F. and Chen Z. 2004. Current microalgal health food R and D activities in China. Hydrobiologia 512:45-48   DOI   ScienceOn
34 Heo S.J., Lee K.W., Song C.B. and Jeon Y.J. 2003. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18: 71-81   DOI   ScienceOn
35 Brand-Williams W., Cuvelier M.E. and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 28: 25-30
36 Benedettia S., Benvenutia F., Pagliarania S., Francoglia S., Scogliob S. and Canestraria F. 2004. Antioxidant properties of a novel phycocyanin extract from the blue-green alga Aphanizomenon flos-aquae Life Sci. 75: 2353-2362   DOI   ScienceOn
37 Kikuzaki H. and Nakatan, N. 1993. Antioxidant effects of some ginger constituents. J. Food Sci. 58: 1407-1410   DOI   ScienceOn
38 Korycka-Dahl M.B. and Richardson T. 1978. Activated oxygen species and oxidation of food constituents. Crit. Rev. Food Sci. Nutr. 10: 209-241   DOI   ScienceOn
39 Ames B. 1998. Micronutrients prevent cancer and delay aging. Toxicol Lett. 102: 5-18   DOI   ScienceOn
40 AOAC.1995. Official Method of Analysis of the Association of Official Analytical Chemists (16th ed.), Virginia, USA