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http://dx.doi.org/10.7314/APJCP.2012.13.10.5273

Chemopreventive Potential of Coumarin in 7,12-dimethylbenz[a] anthracene Induced Hamster Buccal Pouch Carcinogenesis  

Baskaran, Nagarethinam (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Manoharan, Shanmugam (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Karthikeyan, Sekar (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Prabhakar, Murugaraj Manoj (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.10, 2012 , pp. 5273-5279 More about this Journal
Abstract
The aim of the present study was to investigate the chemopreventive effect of coumarin against 7, 12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis by monitoring tumor incidence and histopathological changes as well as by analyzing the status of biochemical markers (lipid peroxidation, enzymatic and non-enzymatic antioxidants, phase I and phase II detoxification enzymes). Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We noted 100% tumor formation with marked abnormalities in the biomarkers status in hamsters treated with DMBA alone. Oral administration of coumarin at a dose of 100 mg/kg body weight (bw) to DMBA treated hamsters completely prevented the tumor formation as well as restored the staus of biochemical variables. The results of the present study thus suggest that the chemopreventive effect of coumarin is probably due to its anti-lipid peroxidative potential and modulating effect on carcinogen detoxification agents in favor of the excretion of ultimate carcinogenic metabolites of DMBA during DMBA-induced hamster buccal pouch carcinogenesis.
Keywords
Antioxidants; chemoprevention; coumarin; detoxification agents; DMBA; oral cancer;
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1 Alias LM, Manoharan S, Vellaichamy L, et al (2009). Protective effect of ferulic acid on 7,12-dimethylbenz[a]anthraceneinduced skin carcinogenesis in Swiss albino mice. Exp Toxicol Pathol, 61, 205-14.   DOI   ScienceOn
2 Anusuya C, Manoharan S (2011). Antitumor initiating potential of rosmarinic acid in 7,12-dimethylbenz(a)anthraceneinduced hamster buccal pouch carcinogenesis. J Environ Pathol Toxicol Oncol, 30, 199-211.   DOI
3 Balakrishnan S, Menon VP, Manoharan S (2008). Ferulic acid inhibits 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. J Med Food, 11, 693-700.   DOI   ScienceOn
4 Baskar AA, Manoharan S, Manivasagam T, Subramanian P (2004). Temporal patterns of lipid peroxidation product formation and antioxidants activity in oral cancer patients. Cell Mol Biol Lett, 9, 665-73.
5 Baskaran N, Manoharan S, Silvan S, et al (2011a). Protective effect of coumarin on cell surface glycoconjugates abnormalities during 7,12-dimethylbenz(a)anthracene (DMBA) induced oral carcinogenesis. Int J Biol Med Res, 2, 643-7.
6 Baskaran N, Rajasekaran D, Manoharan S (2011b). Coumarin protects 7,12-dimethylbenz(a)anthracene-induced genotoxicity in the bone marrow cells of golden Syrian hamsters. Int J Nutr Pharmacol Neurol Dis, 1, 167-73.   DOI
7 Beutler E, Kelly BM (1963). The effect of sodium nitrite on red cell GSH. Experientia, 19, 96-7.   DOI
8 Bhuvaneswari V, Abraham SK, Nagini S (2005). Combinatorial antigenotoxic and anticarcinogenic effects of tomato and garlic through modulation of xenobiotic-metabolizing enzymes during hamster buccal pouch carcinogenesis. Nutrition, 21, 726-31.   DOI
9 Carlberg I, Mannervik B (1985). Glutathione reductase. Methods Enzymol, 113, 484-90.   DOI
10 Chandra Mohan KV, Kumaraguruparan R, Prathiba D, Nagini S (2006). Modulation of xenobiotic-metabolizing enzymes and redox status during chemoprevention of hamster buccal carcinogenesis by bovine lactoferrin. Nutrition, 22, 940-6.   DOI
11 Chang WS, Lin CC, Chuang SC, Chiang HC (1996). Superoxide anion scavenging effect of coumarins. Am J Chin Med, 24, 11-7.   DOI   ScienceOn
12 Desai ID (1984). Vitamin E analysis methods for animal tissues. Methods Enzymol, 105, 138-47.   DOI
13 Ernster L (1967). DT-Diaphorase. In: Estabrook, R.W., Pullman, M.E. (Eds.), Methods Enzymol, vol 10. Academic Press, New York, pp. 309-17.
14 Feuer G, Kellen JA, Kovacs K (1976). Suppression of 7,12-dimethylbenz(alpha)anthracene-induced breast carcinoma by coumarin in the rat. Oncol, 33, 35-9.   DOI
15 Habig WH, Pabst MJ, Jakoby WBC (1974). Glutathione-Stransferases: the first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 7130-9.
16 Krishnakumar N, Manoharan S, Palaniappan PR, et al (2009). Chemopreventive efficacy of piperine in 7,12-dimethyl benz [a] anthracene (DMBA)-induced hamster buccal pouch carcinogenesis: an FT-IR study. Food Chem Toxicol, 47, 2813-20.   DOI
17 Kakkar P, Das B, Viswanathan PN (1984). A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys, 21, 130-2.
18 Khan N, Sharma S, Sultana S (2004). Amelioration of ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and tumor promotion response by coumarin (1,2-benzopyrone) in wistar rats. Cancer Lett, 210, 17-26.   DOI
19 Kowsalya R, Vishwanathan P, Manoharan S (2011). Chemopreventive potential of 18beta-glycyrrhetinic acid: an active constituent of liquorice, in 7,12-dimethylbenz(a) anthracene induced hamster buccal pouch carcinogenesis. Pak J Biol Sci, 14, 619-26.   DOI
20 Lacy A, O'Kennedy R (2004). Studies on coumarins and coumarin-related compounds to determine their therapeutic role in the treatment of cancer. Curr Pharm Des, 10, 3797-811.   DOI   ScienceOn
21 Lowry OH, Rosebrough NJ, Farr AL, Randall, RJ (1951). Protein measurement with folin phenol reagent. J Biol Chem, 193, 265-75.
22 Malik NM, Moore GB, Smith G, et al (2006). Behavioural and hypothalamic molecular effects of the anti-cancer agent cisplatin in the rat: A model of chemotherapy-related malaise?. Pharmacol Biochem Behav, 83, 9-20.   DOI
23 Manoharan S, Kolanjiappan K, Suresh K, Panjamurthy K (2005). Lipid peroxidation & antioxidants status in patients with oral squamous cell carcinoma. Indian J Med Res, 122, 529-34.
24 Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-8.   DOI   ScienceOn
25 Manoharan S, Sindhu G, Vinothkumar V, Kowsalya R (2012). Berberine prevents 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis: a biochemical approach. Eur J Cancer Prev, 21, 182-92.   DOI
26 Manoharan S, Vasanthaselvan M, Silvan S, et al (2010). Carnosic acid: a potent chemopreventive agent against oral carcinogenesis. Chem Biol Interact, 188, 616-22.   DOI   ScienceOn
27 Muwonge R, Ramadas K, Sankila R, et al. (2008). Role of tobacco smoking, chewing and alcohol drinking in the risk of oral cancer in Trivandrum, India: a nested case-control design using incident cancer cases. Oral Oncol, 44, 446-54.   DOI
28 Omura T, Sato R (1964). The carbon monoxide binding pigment of liver microsomes. J Biol Chem, 239, 2370-8.
29 Palan PR, Mikhail MS, Basu J, Romney SL (1991). Plasma levels of antioxidant beta-carotene and alpha-tocopherol in uterine cervix dysplasias and cancer. Nutr Cancer, 15, 13-20.   DOI   ScienceOn
30 Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB (2010). Oxidative stress, inflammation, and cancer: how are they linked?. Free. Radic Biol Med, 49, 1603-16.   DOI
31 Rotruck JT, Pope AL, Ganther HE, et al (1973). Selenium: biochemical role as a component of glutathione peroxidase. Sci, 179, 588-90.   DOI   ScienceOn
32 Shklar G (1982). Oral mucosal carcinogenesis in hamsters: inhibition by vitamin E. J Natl Cancer Inst, 68, 791-7.
33 Tseng A (1991). Chemoprevention of tumors in MTV-H ras transgenic mice with coumarin. Proc Am Assoc Cancer Res, 32, 2257.
34 Silvan S, Manoharan S, Baskaran N, et al (2011). Chemopreventive potential of apigenin in 7,12-dimethylbenz(a)anthracene induced experimental oral carcinogenesis. Eur J Pharmacol, 670, 571-7.   DOI
35 Sinha AK (1972). Colorimetric assay of catalase. Anal Biochem, 47, 389-94.   DOI   ScienceOn
36 Tietze F (1969). Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem, 27, 502-22.   DOI   ScienceOn
37 Vinothkumar V, Manoharan S (2011). Chemopreventive efficacy of geraniol against 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Redox Rep, 16, 91-100.   DOI
38 Von Angerer E, Kager M, Maucher A (1994). Antitumour activity of coumarin in prostate and mammary cancer models. J Cancer Res Clin Oncol, 120, 14-6.   DOI
39 Warnakulasuriya S (2010). Living with oral cancer: epidemiology with particular reference to prevalence and life-style changes that influence survival. Oral Oncol, 46, 407-10.   DOI
40 Warnakulasuriya S (2009). Global epidemiology of oral and oropharyngeal cancer. Oral Oncol, 45, 309-16.   DOI   ScienceOn
41 Wattenberg LW, Lam LK, Fladmoe AV (1979). Inhibition of chemical carcinogen-induced neoplasia by coumarins and alpha-angelicalactone. Cancer Res, 39, 1651-4.
42 Wittgen HG, van Kempen LC (2007). Reactive oxygen species in melanoma and its therapeutic implications. Melanoma Res, 17, 400-9.   DOI
43 Yagi K (1987). Lipid peroxides and human diseases. Chem Phys Lipids, 45, 337-51.   DOI   ScienceOn