• Parasites, Hosts and Diseases
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• v.54 no.1
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• pp.47-53
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• 2016

Echinostomes are intestinal trematodes that infect a wide range of vertebrate hosts, including humans, in their adult stage and also parasitize numerous invertebrate and cold-blooded vertebrate hosts in their larval stages. The purpose of this study was to compare Echinostoma malayanum parasite growth, including worm recovery, body size of adult worms, eggs per worm, eggs per gram of feces, and pathological changes in the small intestine of experimental animals. In this study, 6-8-week-old male hamsters, rats, mice, and gerbils were infected with echinostome metacercariae and then sacrificed at day 60 post-infection. The small intestine and feces of each infected animal were collected and then processed for analysis. The results showed that worm recovery, eggs per worm, and eggs per gram of feces from all infected hamsters were higher compared with infected rats and mice. However, in infected gerbils, no parasites were observed in the small intestine, and there were no parasite eggs in the feces. The volume of eggs per gram of feces and eggs per worm were related to parasite size. The results of histopathological changes in the small intestine of infected groups showed abnormal villi and goblet cells, as evidenced by short villi and an increase in the number and size of goblet cells compared with the normal control group.

• 한국해양학회지
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• v.23 no.4
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• pp.184-193
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• 1989

To study the pelagic fish egg community in the mid-east coastal waters of the Yellow Sea, pelagic eggs were sampled with Bongo net at 13 stations from Chonsu Bay to Keum Estuary from July, 1985 to June, 1986. Nineteen taxa of pelagic eggs were collected. Among them, 7 taxa were identified to the species level, and 2 taxa to the family level. Engraulis japonica was the most abundant species with 89.5% of the total eggs; Sillago japonica was 2.5%; Clupanodon punctatus, 2.4%; Herklotsichthys zunasi, 1.7%; Callionymidae spp., 1.6%. These 5 taxa occupied 97.7% of the total eggs. Pelagic eggs occurred from April to October. In June and July, their abundances were high, but the species diversities were low as E. japonica eggs were predominant. The range of spawning temperature for each taxa were estimated from the occurence pattern of the pelagic eggs.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.429-433
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• 2008

On the leaf disc of fruit vegetables (cucumber, sweet pepper, tomato, egg plant, oriental melon), it has been evaluated that the capability of four predatory mites (Amblyseius swirskii Athias-Henriot, Neoseiulus barkeri (Hughes), N. cucumeris (Oudemans), N. californicus (McGregor)) to prey on the eggs of Bemisia tabaci (Gennadius) and to lay own eggs. The most aggressive mite was A. swirskii which had consumed about $9{\sim}12$ eggs in a day. The quantity of eggs consumed by N. barkeri, N. cucumeris and N. californicus was about $6{\sim}9,\;4{\sim}6$ and $1{\sim}4$, respectively. The most productive mite was also A. swirskii which had laid about $1.2{\sim}1.9$ eggs in a day. The others could not lay more than 0.5 eggs in a day.

• Korean Journal of Ichthyology
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• v.32 no.1
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• pp.14-20
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• 2020

The purpose of this study is to clarify when spawning occurs of Clupea pallasii in the coastal waters of Jinhae Bay and Yeongun-ri, Tongyeong, Korea from December to February using SCUBA observation which is known as spawning season. Eighteen surveys of SCUBA diving observations have performed at 3 stations of Jinhae Bay from December 2017 to January 2018 and 24 times of survey at Yeongun-ri, Tongyeong from December 2017 to February 2018. In Jinhae Bay, average 740,274 eggs/㎡ fertilized eggs were observed in Judo Island and average 671,718 eggs/㎡ were observed in Gusan-myeon. On December 30, 2017, about 388,444 eggs/㎡ fertilized eggs were observed in Yeongun-ri, Tongyeong but no eggs were founded in next surveys. The results in this study suggest that spawning season of C. pallasii in the coastal waters of Jinhae Bay and Tongyeong begins in late December at average 7.7℃ water temperature.

• Korean Journal of Poultry Science
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• v.28 no.3
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• pp.225-229
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• 2001

This experiment was carried out to investigate the effects of storage temperature and time on hatch-ability of the commercial Ross broiler breeder eggs. In Trial 1,720 eggs collected in the morning were allotted to four storage time treatments (3, 5, 7 and 9 days), with three replicates per treatment. The storage temperature was fixed at $4^{\circ}C$. In Trials 2 and 3, similar protocol was used as in Trial 1 except that the storage temperature was fixed at $17^{\circ}C$ and $28^{\circ}C$respectively. In Trial 1, hatchability was significantly (P<0.05) reduced when the eggs were stored more than 3 days. In Trial 2, no significant difference was found among all treatments, indicating that, at 17$^{\circ}C$ storage temperature, the eggs could be stored up to 9 days without reducing hatchability. In Trial 3, however, hatchability of eggs stored for 9 days was significantly reduced compared to the other treatments. In conclusion, we found out that optimum hatchability can be achieved with a storage temperature of $17^{\circ}C$ for Ross broiler eggs stored for up to 9 days.

• Korean Journal of Ichthyology
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• v.5 no.2
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• pp.217-225
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• 1993

A study on the distribution and mortality of eggs and larvae of anchovy. Engraulis japonica, was carried out on the basis of data collected from ichthyoplankton survey in the southern coastal waters of Korea from June to August in 1990. Egg and larval distribution areas were shifted from the adjacent waters of Namhae Is, to the eastern waters of Goeje Is, while eggs and larvae were developed in June. However, high densities of eggs and larvae of anchovy and were found near Goeje Is. in July. Anchovy eggs and larvae were correlated significantly with zooplankton in July, and were not in June and August. It is likely that the low instantaneous mortality coefficients in eggs and high values in the larvae during June and high values in eggs and low values in the larvae during July were affected by the distribution pattern of zooplankton.

• Development and Reproduction
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• v.22 no.3
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• pp.289-295
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• 2018

Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica. Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl, respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels. Expressions of yolk processing enzyme genes did not show significant difference between floating and sinking egg groups. Expression of ctsb decreased when fertilization rate was high. Expression of ctsd, ctsl and lpl, however, did not show any significant differences. These results suggest that ctsb expression could be an indicator of egg quality, and that some proteins prone to be digested by ctsb could be very important in the process of fertilization and normal cleavage in this species. Further study should identify these critical proteins to improve our understanding on the quality of fish eggs.

• Development and Reproduction
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• v.24 no.1
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• pp.19-30
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• 2020

The pH of water is one of the main environmental factors exerting selective pressure on marine and freshwater organisms. Here, we focus on the influence of pH on an organism's ability to maintain homeostasis and investigate the effects of acidification on immunity-related genes and osmotic pressure during early development of the olive flounder, Paralichthys olivaceus. The aim of our study was to determine the influence of various pH levels on the fertilized eggs and larvae of P. olivaceus. Gametes of P. olivaceus were artificially introduced and the resulting fertilized eggs were incubated at pH 4.0 (low), 6.0, and 8.0 (equivalent to natural sea water; control). We found that all eggs sank from the water column at pH 4.0. After 38 h, these eggs showed slow development. Hatching occurred more slowly at pH 4.0 and 6.0 and did not occur at all at pH 4.0. Result of gene expression, caspase and galectin-1 were expressed from the blastula to pre-hatch stages, with the exception of the two-cell stage. HSP 70 was also steadily expressed at all pH levels over the five days. The osmolality of fertilized eggs differed marginally at each stage and across pH levels. So, this results demonstrates that low pH level is detrimental to P. olivaceus fertilized eggs.

• Journal of Biosystems Engineering
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• v.33 no.5
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• pp.348-354
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• 2008

Egg grading is determined by exterior and interior quality. Among the evaluation methods for the egg quality, a candling method is common to identify eggs with cracked shells and interior defects. But this method is time-consuming and laborious. In addition, practically, it is challenging to detect hairline and micro cracks. In this study, an on-line inspection system based on acoustic resonance frequency analysis was developed to detect hairline cracks on eggshells. A roller conveyor was used to transfer eggs along one lane to the impact position where each of eggs rotated by the roller was excited with an impact device at four different locations on the eggshell equator. The impact device was consisted of a plastic hammer and a rotary solenoid. The acoustic response of the egg to the impact was measured with a small condenser microphone at the same position as the impact device was installed. Two acoustic parameters, correlation coefficient for normalized power spectra and standard deviation of peak resonant frequencies, were used to detect cracked eggs. Intact eggs showed relatively high correlations among the four normalized power spectra and low standard deviations of the four peak resonant frequencies. On the other hand, cracked eggs showed low correlations and high standard deviations as compared to the intact. This method allowed a crack detection rate of 97.6%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.139-144
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• 1988

The vertical distribution of eggs and larvae of anchovy. Engraulis japonica were studied based on ichthyoplankton and the temperature distribution in the southwestern part of the Sea of Japan off Korea during the summer of 1985 and 1986. Thermoclines occurred at the surface layer in the coastal area and at the sub-surface layer in the offshore area in the early summer of 1985. However, they occurred at the surface layer throughout the summer of 1986. Anchovy eggs and larvae were abundant in the offshore in 1985 and in the coastal area in 1986. It seems that the spawning ground of anchovy in 1985 shifted to the offshore owing to the sloping of thermoclines. According to the distribution layer of anchovy eggs by developmental stage, the eggs in the early developmental stage were abundant at $0\~30m$ layer about 06:00. But the eggs in the late developmental stage were abundant at $30\~100m$ loom layer about 24 : 00. These results suggest that anchovy spawn at the $0\~30m$ layer at night and eggs tend to sink to the $30\~100m$ layer in the course of development.