Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Influence of Osmolality and Acidity on Fertilized Eggs and Larvae of Olive Flounder (Paralichthys olivaceus)

  • Kim, Ki-Hyuk (Dept. of Marine Life Science, Jeju National University) ;
  • Moon, Hye-Na (Dept. of Marine Life Science, Jeju National University) ;
  • Noh, Yun-Hye (Dept. of Marine Life Science, Jeju National University) ;
  • Yeo, In-Kyu (Dept. of Marine Life Science, Jeju National University)
  • Received : 2020.02.10
  • Accepted : 2020.02.28
  • Published : 2020.03.31
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Abstract

The pH of water is one of the main environmental factors exerting selective pressure on marine and freshwater organisms. Here, we focus on the influence of pH on an organism's ability to maintain homeostasis and investigate the effects of acidification on immunity-related genes and osmotic pressure during early development of the olive flounder, Paralichthys olivaceus. The aim of our study was to determine the influence of various pH levels on the fertilized eggs and larvae of P. olivaceus. Gametes of P. olivaceus were artificially introduced and the resulting fertilized eggs were incubated at pH 4.0 (low), 6.0, and 8.0 (equivalent to natural sea water; control). We found that all eggs sank from the water column at pH 4.0. After 38 h, these eggs showed slow development. Hatching occurred more slowly at pH 4.0 and 6.0 and did not occur at all at pH 4.0. Result of gene expression, caspase and galectin-1 were expressed from the blastula to pre-hatch stages, with the exception of the two-cell stage. HSP 70 was also steadily expressed at all pH levels over the five days. The osmolality of fertilized eggs differed marginally at each stage and across pH levels. So, this results demonstrates that low pH level is detrimental to P. olivaceus fertilized eggs.

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References

  1. Bell MA, Foster SA (1994) Introduction to the evolutionary biology of the threespine stickleback. In: Bell MA, Foster SA (eds), The Evolutionary Biology of the Threespine Stickleback. Oxford University Press, Oxford, UK, pp 1-27.
  2. Bonga SEW, Dederen LHT, (1986) Effects of acidified water on fish. Endeavour 10:198-202. https://doi.org/10.1016/0160-9327(86)90094-3
  3. Brown DJA, Lynam S (1981) The effect of sodium and calcium concentrations on the hatching of eggs and the survival of the yolk sac fry of brown trout, Salmo trutta L. at low pH. J Fish Biol 19:205-211. https://doi.org/10.1111/j.1095-8649.1981.tb05824.x
  4. Carline RF, DeWalle DR, Sharpe WE, Dempsey BA, Gagen CJ, Swistock B (1992) Water chemistry and fish community responses to episodic stream acidification in Pennsylvania, USA. Environ Pollut 78:45-48. https://doi.org/10.1016/0269-7491(92)90008-X
  5. Checkley DM, Dickson AG, Takahashi M, Radich JA, Eisenkolb N, Asch R (2009) Elevated $CO_2$ enhances otolith growth in young fish. Science 324:1683. https://doi.org/10.1126/science.1169806
  6. Daye PG, Garside ET (1980) Structural alterations in embryos and alevins of the Atlantic salmon, Salmo salar L., induced by continuous or short-term exposure to acidic levels of pH. Can J Zool 58:27-43. https://doi.org/10.1139/z80-004
  7. Dixson DL, Munday PL, Jones GP (2010) Ocean acidification disrupts the innate ability of fish to detect predator olfactory cues. Ecol Lett 13:68-75. https://doi.org/10.1111/j.1461-0248.2009.01400.x
  8. Duarte RM, Ferreira MS, Wood CM, Val AL (2013) Effect of low pH exposure on $Na^+$ regulation in two cichlid fish species of the Amazon. Comp Biochem Physiol Part A: Mol Integr Physiol 166:441-448. https://doi.org/10.1016/j.cbpa.2013.07.022
  9. Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: Dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97-177. https://doi.org/10.1152/physrev.00050.2003
  10. Evans DH (2011) Freshwater fish gill ion transport: August Krogh to morpholinos and microprobes. Acta Physiol 202:349-359. https://doi.org/10.1111/j.1748-1716.2010.02186.x
  11. Fabry VJ (2008) Marine calcifiers in a high-$CO_2$ ocean. Science 23:1020-1022. https://doi.org/10.1126/science.1157130
  12. Fabry VJ, Seibel BA, Feely RA, Orr JC (2008) Impacts of ocean acidification on marine fauna and ecosystem processes. ICES J Mar Sci 65:414-432. https://doi.org/10.1093/icesjms/fsn048
  13. Feely RA, Doney SC, Cooley SR (2009) Ocean acidification: Present conditions and future changes in a high-$CO_2$ world. Oceanography 22:36-47. https://doi.org/10.5670/oceanog.2009.95
  14. Foshtomi MY, Abtahi B, Sari AE, Taheri M (2007) Ion composition and osmolarity of Caspian Sea ctenophore, Mnemiopsis leidyi, in different salinities. J Exp Mar Biol Ecol 352:28-34. https://doi.org/10.1016/j.jembe.2007.06.029
  15. Gattuso JP, Hansson L (2011) Ocean acidification: Background and history. In: Jean-Pierre G, Lina H (eds), Ocean Acidification. Oxford University Press, Oxford, UK, pp 1-20.
  16. Heisler N (1986) Acid-Base Regulation in Animals. Elsevier, New York, NY.
  17. Henriksen A, Skogheim OK, Rosseland BO (1984) Episodic changes in pH and aluminiumspeciation kill fish in a Norwegian salmon river. Vatten 40:255-260.
  18. Hwang PP, Lee TH, Lin LY (2011) Ion regulation in fish gills: Recent progress in the cellular and molecular mechanisms. Am J Physiol Regul Integr Comp Physiol 301:R28-R47. https://doi.org/10.1152/ajpregu.00047.2011
  19. Jang MS, Lee YM, Yang H, Lee JH, Noh JK, Kim HC, Park CJ, Park JW, Hwang IJ, Kim SY (2013) Gene analysis of galectin-1, innate immune response gene, in olive flounder Paralichthys olivaceus at different developmental stage. J Fish Pathol 26:255-263. https://doi.org/10.7847/jfp.2013.26.3.255
  20. Kim KS, Shim JH, Kim S (2015) Effects of $CO_2$-induced ocean acidification on the growth of the larval olive flounder Paralichthys olivaceus. Ocean Sci J 50:381-388. https://doi.org/10.1007/s12601-015-0035-z
  21. Knutzen J (1981) Effects of decreased pH on marine organisms. Mar Pollut Bull 12:25-29. https://doi.org/10.1016/0025-326X(81)90136-3
  22. Lee RM, Gerking SD, Jezierska B (1983) Electrolyte balance and energy mobilization in acidstressed rainbow trout, Salmo gairdneri, and their relation to reproductive success. Environ Biol Fish 8:115-123. https://doi.org/10.1007/BF00005178
  23. Michael K, Kreiss CM, Hu MY, Koschnick N, Bickmeyer U, Dupont S, Lucassen M (2016) Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. Comp Biochem Physiol B Biochem Mol Biol 193:33-46. https://doi.org/10.1016/j.cbpb.2015.12.006
  24. McDonald DG (1983) The effects of $H^+$ upon the gills of freshwater fish. Can J Zool 61:691-703. https://doi.org/10.1139/z83-093
  25. McWilliams PG, Potts WTW (1978) The effects of pH and calcium concentrations on gill potentials in the brown trout, Salmo trutta. J Comp Physiol B Biochem Syst Environ Physiol 126:277-286. https://doi.org/10.1007/BF00688938
  26. Peterson RH, Daye PG, Metcalfe JL (1980) Inhibition of Atlantic salmon (Salmo salar) hatching at low pH. Can J Fish Aqua Sci 37:770-774. https://doi.org/10.1139/f80-103
  27. Scott DM, Lucas MC, Wilson RW (2005) The effect of high pH on ion balance, nitrogen excretion and behaviour in freshwater fish from an eutrophic lake: A laboratory and field study. Aquat Toxicol 73:31-43. https://doi.org/10.1016/j.aquatox.2004.12.013
  28. Shartau RB, Brix KV, Brauner CJ (2017) Characterization of $Na^+$ transport to gain insight into the mechanism of acid-base and ion regulation in white sturgeon (Acipenser transmontanus). Comp Biochem Physiol A Mol Integr Physiol 204:197-204. https://doi.org/10.1016/j.cbpa.2016.12.004
  29. Shu L, Laurila A, Rasanen K, (2015) Acid stress mediated adaptive divergence in ion channel function during embryogenesis in Rana arvalis. Sci Rep 5:14201. https://doi.org/10.1038/srep14201
  30. Tseng YC, Hu MY, Stumpp M, Lin LY, Melzner F, Hwang PP (2013) $CO_2$-driven seawater acidification differentially affects development and molecular plasticity along life history of fish (Oryzias latipes). Comp Biochem Physiol A Mol Integr Physiol 165:119-130. https://doi.org/10.1016/j.cbpa.2013.02.005