• Mycobiology
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• 제35권2호
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• pp.82-86
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• 2007

Ergone, a fungal metabolite derived from ergosterol, was previously isolated and identified from Polyporus umbellatus. Ergone is a major component of P. umbellatus known to have anti-aldosteronic diuretic effect and also displays cytotoxic activities. Most of mushroom's fruit bodies used for test contained less than 10 ${\mu}g/g$ of ergone. But P. umbellatus have larger amount of ergone than any other mushrooms. In order to improve the ergone production from the submerged culture of P. umbellatus, several factors including medium composition, culture conditions (temperature and pH) and different combinations of co-cultivation with various mycelia were studied. Among various carbon sources examined, starch proved to be most effective for the production of mycelia. The optimum pH and temperature for a flask culture of P. umbellatus mycelia were found to be 4.5 and $25^{\circ}C$, respectively. Under the optimized culture conditions, both the ergone production (86.9 ${\mu}g/g$) and mycelial growth (3.5 g/l) increased when P. umbellatus was cultured with Armillariella mellea. When the optimized conditions were applied, both mycelium and ergone production were significantly enhanced.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.195-201
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• 2007

This study investigated the effects of various culture parameters (carbon sources, temperature, initial pH of culture, NaCl concentration, and light) on the growth and canthaxanthin production by Dietzia natronolimnaea HS-1. The results showed that the most effective carbon source for growth and canthaxantin production was glucose, and the best pH and temperature were 7 and $31^{\circ}C$, respectively. In addition, the biomass and canthaxanthin production increased in a medium without NaCl and in the presence of light. Under the optimized conditions, the maximum biomass, total carotenoid, and canthaxanthin production were $6.12{\pm}0.21g/l,\;4.51{\pm}0.20mg/l,\;and\;4.28{\pm}0.15mg/l$, respectively, in an Erlenmeyer flask system, yet increased to 7.25 g/l, 5.48 mg/l, and 5.29 mg/l, respectively, in a batch fermenter system.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.379-381
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• 2021

정보통신기술이 점차 발전함에 따라 기존의 생산 시스템에 네트워크 및 컴퓨팅 기술을 적용하여 효율적인 생산 환경을 유지하는 연구가 많이 진행되고 있다. 고전적인 제어 시스템과 달리 이러한 스마트 생산 시스템은 생산 환경에 대한 데이터를 실시간으로 수집하여 통계화하고, 그 변화에 따라 유동적으로 작동할 필요가 있다. 본 논문에서는 네트워크 기반의 센서를 통해 실시간으로 데이터를 수집하고, 환경 변화에 효과적으로 대응할 수 있는 계측 시스템을 제시한다. 또한 이를 스마트팜에 적용하여 외부의 환경 변화에 대처하고 효과적인 생산 환경을 유지할 수 있음을 확인하였다.

• 국제학술발표논문집
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• The 7th International Conference on Construction Engineering and Project Management Summit Forum on Sustainable Construction and Management
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• pp.263-270
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• 2017

The precast concrete (PC) structure has been preferred for reasons of shortening the construction time and securing the quality and, in particular, it has a cost saving effect in case of long span and heavy loaded structure with high floor height. Most engineers take it for granted to install the plant produced PC members. Researchers in several papers have argued that slander PC members such as columns and girders can be cost-effective if in-situ production is possible, while ensuring quality equal to or better than in-plant production. However, this argument is not officially accepted because objective verification has not been done. Therefore, the purpose of this study is to experimentally conduct in-situ production of PC members to verify the above claim. For this study, a storage building with long span and heavy loaded structure with high floor height was selected as a case study site. For the site, most of the PC members were supplied from the plant, and some of the columns were produced in the site for this study. As a result, it has been confirmed that it has a cost saving effect of 20% while having superior quality to plant-produced PC columns.

• 시스템엔지니어링학술지
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• 제20권spc1호
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• pp.30-44
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• 2024

The DMAIC methodology, which is most familiar to battery production system developers, is partially inadequate in its conformity to utilize battery production system tasks, so it is necessary to improve the function and structure of the methodology, but many battery production system developers use the DMAIC method based on experience, causing side effects such as confusion, delay in tasks, and insufficient performance during tasks. Accordingly, we intend to conduct an empirical study to improve the "efficiency improvement and conformity evaluation method" so that the DMAIC methodology can be used more reasonably and easily. Using the three-stage research model, we derive components that affect conformity through literature and questionnaire surveys in the first stage, use relational characteristics between components in the second stage to confirm the effect on conformity, and use the relational characteristics in the third stage to confirm the possibility of improving efficiency by applying them to the DMAIC methodology in actual cases. Finally, the "Conformity Assessment Index (CAI) equation" based on relational characteristics is established to enable effective conformity evaluation and continuous improvement.

• 지질공학
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• 제29권4호
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• pp.367-381
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• 2019

We investigate pore pressure conditions and reservoir compaction associated with oil and gas production using 3 different permeability models, which are all based on one-dimensional radial flow diffusion model, but differ in considering permeability evolution during production. Model 1 assumes the most simplistic constant and invariable permeability regardless of production; Model 2 considers permeability reduction associated with reservoir compaction only due to pore pressure drawdown during production; Model 3 also considers permeability reduction but due to the effects of both pore pressure drawdown and coupled pore pressure-stress process. We first derive a unified stress-permeability relation that can be used for various sandstones. We then apply this equation to calculate pore pressure and permeability changes in the reservoir due to fluid extraction using the three permeability models. All the three models yield pore pressure profiles in the form of pressure funnel with different amounts of drawdown. Model 1, assuming constant permeability, obviously predicts the least amount of drawdown with pore pressure condition highest among the three models investigated. Model 2 estimates the largest amount of drawdown and lowest pore pressure condition. Model 3 shows slightly higher pore pressure condition than Model 2 because stress-pore pressure coupling process reduces the effective stress increase due to pore pressure depletion. We compare field data of production rate with the results of the three models. While models 1 and 2 respectively overestimates and underestimates the production rate, Model 3 estimates the field data fairly well. Our result affirms that coupling process between stress and pore pressure occurs during production, and that it is important to incorporate the coupling process in the permeability modeling, especially for tight reservoir having low permeability.

• KSBB Journal
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• 제20권6호
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• pp.447-452
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• 2005

본 연구에서는 과일 폐액으로부터 고정화 세포를 이용하여 수소생산을 위한 배양조건을 조사하였다. 각종 과일 폐액 중에 수박 폐액에서 환원당의 함량이 가장 높았으며, 수소생산량은 2319.2 mL/L이었다. 고정화 물질 sodium alginate의 농도와 크기에 따른 수소생산성 효과는 검토범위 내에서는 적었다. 고정화된 비드의 내부 관찰에서 세포가 왕성하게 생육하고 있음을 확인했다. 대사의 효소물질로 이용될 수 있는 각종 아미노산의 첨가는 종류에 관계없이 수소생산성에 영향을 거의 미치지 않았다. 금속이온 $FeSO_4$를 첨가한 결과 최적 농도는 1.2 g/L이고, 1.3배의 수소생산 증가를 나타났다. 수소생산정지 후 배양액의 유기산은 lactic acid와 butyic acid가 가장 많았다.

• 한국콘텐츠학회논문지
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• 제6권5호
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• pp.66-75
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• 2006

세계적인 애니메이션산업의 호황에도 불구하고 현재 국내에서는 여러 가지 많은 어려움에 봉착해있다. 2003년 개봉된 원더플데이즈 (Wonderful Days)의 흥행실패 이후 외부투자는 거의 끊어지다시피 했으며 각종 정부지원마저도 줄어들고 있다. 이러한 현실에서 애니메이션 제작자들에게는 기존의 제작시스템을 면밀히 점검하고 개선사항들에 대해서는 과감하게 칼을 들이대는 구조조정이 요구되고 있다. 기획력의 부재 등 과거부터 이어져온 고질적인 문제들과 더불어 제작시스템의 개선을 통한 일정단축과 비용절감도 중요한 문제이기 때문에 본 논문은 현재 업계에서 적용되고 있는 전체 3D 애니메이션 제작과정을 살펴보고, 문제점을 분석하여 애니메이션의 효율적인 제작시스템 여건을 위한 제작관리프로그램의 필요성을 제시하는데 그 목적이 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.45-51
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• 2007

A growth trial (Expt. 1) and an in vitro fermentation experiment (Expt. 2) were conducted to determine the response of growth performance, cecal fermentation characteristics and in vitro gas production to incremental levels of substitution of digestible fiber for lignified fiber in the diet of weaned rabbits. Three diets, formulated by substituting soyhulls (SH; used as digestible fiber source) for soybean straw (used as lignified fiber source) at substitution levels of 0, 25 and 50%, were used in a factorial design. In the growth trial (Expt. 1), increasing levels of SH substitution resulted in a quadratic increase in daily body weight gain rate (p<0.04) and feed conversion efficiency (p<0.02), but in a numerical decrease in dietary DM intake (p=0.15). When SH were included in the diet at 25% substitution level, rabbits had the highest rate of liveweight gain and feed conversion efficiency. As SH substitution level increased, pH values and ammonia-N of cecal contents linearly (p<0.001) decreased, but total VFA concentration linearly (p<0.03) increased. With incremental levels of SH substitution, the percentage of acetate and butyrate linearly (p<0.05) reduced, but the percentage of propionate and minor acids linearly (p<0.03) increased. Increasing the SH substitution levels tended to increase incidence of diarrhea. In the in vitro fermentation experiment (Expt. 2), regardless of origin of substrates fermented, increasing SH substitution level resulted in increased maximal gas production (p<0.001) and shortened gas production lag time, but had no effect on gas production rate (p>0.2). These observations suggest that incrementally feeding SH to rabbits could stimulate their cecal microbial activity, allowing cecal fermentation to shift towards favoring fiber digestion. In conclusion, digestible fiber from soyhulls may partially substitute for more lignified fiber, soybean straw, without having an adverse effect on cecal fermentative and microbial activity and growth performance. For growing rabbits, about 73% of total dietary NDF should be supplied by effective NDF, the remainder could come from digestible NDF, such as soyhulls.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.13-13
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• 2014

Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.