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Hydrogen Production from Fruit Wastes by Immobilized Cells of Enterobacter cloacae VJ-1  

Lee, Ki-Seok (Department of Environmental Engineering, Chonnam National University)
Huh, Yang-Il (Department of Environmental Engineering, Chonnam National University)
Chung, Seon-Yong (Department of Environmental Engineering, Chonnam National University)
Kang, Chang-Min (Department of Environmental Engineering, Chodang University)
Publication Information
KSBB Journal / v.20, no.6, 2005 , pp. 447-452 More about this Journal
Abstract
The hydrogen production using immobilized cellsl was conducted using fruit wastewaters at various culture conditions. Three kinds of fruit wastewaters, melon, watermelon and pear were used. Sodium alginate was used as immobilization material. Among them, concentration of reducing sugar which was one of the main components in fruit was the highest at watermelon wastewater, and also hydrogen production was the highest as 2319.2 mL/L in it. Although hydrogen production was not much changed according to sodium alginate concentration, its production was the most at 3%(w/v). As bead size as small, hydrogen production was higher. With inspection of interior, it confirmed that the cell grew well in bead. But the addition of amino acids using as agent for metabolite production had almost no affected on hydrogen productivity. The effective range of $FeSO_4$ addition on hydrogen production were up to 1.2 g/L, and above the concentration, it inhibited the productivity. Organic acids produced during watermelon fermentation were mainly lactic acid, butyric acid, abd acetic acid; and a little of propionic acid.
Keywords
Hydrogen production; immobilization; organic wastewater; $FeSO_4$ concentration;
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1 Bollinger, R., H. Zurrer, and R. Bachofen (1985), Photoproduction of molecular hydrogen from wastewater of a sugar refmery by photosynthetic bacteria, Appl. Microbiol. Biotechnol. 23, 147-151   DOI   ScienceOn
2 Pimental D., I. L. Bennett, and C. Cooney (1983), Solar Energy, 30, 1   DOI   ScienceOn
3 George, R. S. and R. C. Clark (1983), Laboratory-produced microbial polysaccharide has many potential food applications as a gelling, stabilizing, and texturing agent, Food Tech. 4, 63-70
4 ADHA. AWWA and WPCF (1995), Standard method for the examination of water and wastewater, 16th ed
5 Cheetham. P. S. J., K. W. Blunt, and C. Bucke (1979), Physical studies on cell immobilization using calcium alginate gels, Biotechonol. Bioeng. 21, 2155-2168   DOI
6 Lim, D. J., B. J. Kim, S. K. Bae, J. D. Kim, and J. Y. Kong (1999), Immobilization of agarase for the agarooligosaccharide production. Kor. J. Appl. Microbiol. Biotechonol. 27, 208-214
7 Hannoun. B. J. M and G. stephanpoulos (1986), Diffusion coefficients of glucose and ethanol in cell-free and cell-occupied calcium alginate membranes, Biotechnol. Bioeng. 28, 829-835   DOI   ScienceOn
8 Tanaka, H., M. Matsumura, and I. A. Veliky (1984), Diffusion characteristics of substrates in Ca-alginate gel beads, Biotechonol. Bioeng. 26, 53-58   DOI   ScienceOn
9 Miyake. J., Y. Asada, and S. Kawamura (1989), Nitrogenase In: O. Kitani and C. W. Hall Eds., Biomass handbook, New York, Gordon and Breach Science Publishers, 362-370
10 Nagai, S., T. Kodama, K. Ohmiya, K. Miyamoto, S. Yokoyama, and H. Saiki (1996), Interim evaluation report of development of environmentally friendly technology for the production of hydrogen, NEDO, Tokyo, Japan
11 Leenen, J. E., A. P. Vitor, C. F. G. Katja, and T. Johannes (1996), Characteristics of and selection criteria for support materials for cell immobilization in wastewater treatment. Wat. Res. 30, 2985-2996   DOI   ScienceOn
12 Britz, M. L., N. Simonov, and U. H. Chun (1997), Immobilized luminescent cell-based flow through monitoring of environmental pollutants, J. Microbiol. Biotechnol. 7, 250-257
13 Jones, D. T. and D. R. Woods (1986), Acetone-butanol fermentation revisited, Microbiol. Rev. 50, 484-524
14 Vignais, P. M., A. Colbeau, J. C. Wilson, and Y. Jouanneau (1985), Hydrogenase, nitrogenase and hydrogen metabolism in the photosynthetic bacteria, Advances in Microbial Physiology 26, 155-234   DOI
15 Cimburkova, E., J. Zima, J. Novak, and Z. Vanek (1988), Nitrogen regulation avermectins biosynthesis in Steptomyces avermitilis in a chemically defined, J. Basic Microbiol. 28, 491-499   DOI
16 Demain, A. L. and N. A. Solomon (1986), Substrates for Large-scale fermentations, Manual of Industrial Microbiology, 130-131
17 Sawada, H. and P. L. Rogers (1977), Phtosynthetic bacteria in waste treatment: pure culture studies, J. Ferment. Technol. 55, 297-310
18 Kondratieva. E. N. and I. N. Gogotov (1983), Production of molecular hydrogen in microorganisms, Adv. Biochem. Eng. 28, 139-191
19 Zajic, J. E., N. Kosaric, and J. D. Brosseau (1978), Microbial production of hydrogen, In A. Vol. 9. Fiechter Eds, Advances in Biochemcal Engineering, pp57-109, Springer-Verlag, Berlin
20 Lee, K. S., C. M. Kang, and S. Y. Chung (2003). Isolation and characterization of hydrogen production bacterium, Korean J. Biotechnol. Bioeng. 18, 149-154
21 Eikmeier, H. and H. J. Rehm (1987) Stability of Ca-alginate during citric acid production of immobilized Aspergillus niger. Appl. Microbiol. Biotechnol. 26, 105-111   DOI
22 Leenan, E. J. T. M., V. A. P. Dos Santos, K. C. F. Grolle, J. Tramper, and R. H. Wijffels (1996), Characteristics of and selection criteria for support materials for cell immobilization in wastewater treatment, Wat. Res. 30, 2985-2996   DOI   ScienceOn