• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.210-216
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• 2021

This study aimed to enhance ethanol productivity of Saccharomyces cerevisiae through genome editing using CRISPR/CAS9. To increase ethanol productivity, ACC1, ELO1, and OLE1 were overexpressed in S. cerevisiae using the CRISPR/CAS9 system. The strains overexpressing ACC1, ELO1, and OLE1 survived up to 24 h in YPD medium supplemented with 18% ethanol. Moreover, the ethanol yields in strains overexpressing ACC1 (428.18 mg ethanol/g glucose), ELO1 (416.15 mg ethanol/g glucose), and OLE1 (430.55 mg ethanol/g glucose) were higher than those in the control strains (400.26 mg ethanol/g glucose). In conclusion, the overexpression of these genes increased the viability of S. cerevisiae at high ethanol concentrations and the ethanol productivity without suppressing glucose consumption.

• The Korean Journal of Archival Studies
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• no.2
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• pp.129-168
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• 2000

$Chos{\check{o}}n$ Dynasty was governed by the words of king, however, if they were not announced by the form of public document, they were useless. Therefore, the form of public document was the symbol of governing activity, and it was very important to manage the public record in operating the nation affairs. However, the point we should consider is that $Chos{\check{o}}n$ Dynasty, in the case of managing the public record, edited 'the original' instead of preserving them. And so, the preservation of the public record was deeply related to the editing of history. In Late Korea dynasty, the management of the public record got into utter confusion and so many troubles were occurred. In order to resolve these problems, the movements which innovated the public record managements system was gradually extended. This movements were continued in $Chos{\check{o}}n$ Dynasty. Finally, through the several modification procedures, the rules of public record management were legislated by 'Kyong Kuk Dae Jeon'(經國大典). Especially, by laws of 'Kyong Kuk Dae Jeon', not only the daily records of Secretary Department(承政院) but also the important documents which was related to the government branch should be booked in the last decade of the year and the number of the books had to be reported to the king. This rule made certification of the fact that the king was the last confirmer of the public record management. In addition, through the procedure of printing the record of the national affairs, and the diplomatic once in three years, and then preserving that public record in the National Department(議政府), other departments responsible and the archives, the National Department practically took part in the public record management. The management system was also changed in order that the public record was virtually used and consulted before taking the procedure of immortal preservation. All public documents were classified by the definite rule and filed in a same volume. After classifying and filing, certainly, for making convenience of reference and application of public record, records were indicated by the paper card. It may be that, after the step of reference, application and indicating, the public documents were gradually transferred to the next step. In these procedures, Early $Chos{\check{o}}n$ public documents system was confirmed.

• Journal of Korea Game Society
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• v.19 no.3
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• pp.43-52
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• 2019

Advances in technology are enhancing the player's visual satisfaction in games. Reflexively, narrative satisfaction has not been highlighted. This led to a player's critical view of the absence of original games. Especially in gameplay, quests require repeat and similar content and imperative tasks. It is overlooked that a quest can cause players to produce various meanings and effects. The concept of editing ability to reorganize the designed rules of the player beyond the performance and behavioral rules is conceptualized, and the four types of players that operate the characteristics can see that the player can intensify game play and immerse himself in play saw.

• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.305-314
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• 2018

'New Breeding Techniques (NBTs)' have been one of hot issues, since their future will be affected profoundly by national as well as international regulatory landscapes. In this review, we compare characteristics of NBTs with conventional and genetic modification, and analyze genetically modified organism (GMO) regulatory systems in the context of possible regulation of NBTs. NBTs are very heterogeneous in terms of principles, methodologies, and final products. As Living Modified Organisms (LMO) is defined in the Cartagena Protocol on Biosafety (CPB) as an organism containing novel combination of genetic materials obtained by the use of modern biotechnology, CPB as well as other national legislations locate itself somewhere in the middle between product-based and process-based regulations. It is also noted that jurisdictions with regulatory systems more oriented to product-based one tend to be more productive and decide or may decide to exempt site-directed nucleases-1 from GMO regulation. In this context, Korean legislations are reviewed to clarify the commons and differences in GMO definitions. Act on Transboundary Movement of LMO Act, Food Sanitation Act and Agricultural and Fishery Products Quality Control Act are three major acts to regulate GMOs. It is noted that there are differences in the definition of LMO or GM food/products especially between the LMO Act and the Food Sanitation Act. Such differences may cause conflicts between Acts when policy-decision regarding the regulation of NBTs is made. Therefore, it is necessary to reorganize legislations before policies regarding the regulation of any techniques from biotechnology are made.

• Journal of Digital Convergence
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• v.16 no.12
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• pp.555-561
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• 2018

It is the Korean language textbook of elementary school 1~2 grade that forms the basis of the textbook to nurture the creative convergence talent with the core competence required by the future society. In order to increase the learning effect based on the textbooks, the interest inducing factors were derived on the basis of the learning interest, and the textbook design was analyzed by the in - depth interviews and discussions of the expert group. As a result, Graphic elements using bright and soft colors, illustrations of peer groups related to learning contents, and resilient use of sans serifs. However, issues such as lack of proper mixing of photos and illustrations, further development of learning helper characters, configuration of spare margins, graphic image design, and lack of a structured layout that utilizes color and visual images were cited as problems.

• Korean Journal of Heritage: History & Science
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• v.44 no.2
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• pp.26-45
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• 2011

The Gongchengzuofazeli, which was published in 1734, the 12th year of the Yongzheng (雍正) emperor of the Ching Dynasty, is an authoritative book that discusses the architectural technology and engineering practices at that time. Moreover, buildings constructed during the Ching Dynasty are substantially similar with the book's content. This study is important in that it is done as the first step in conducting a comparative analysis between the Korean and Chinese wood structure mechanism. First, each content of the book is examined according to the editing order. And second, the standard for division between Da-shi (大式) and Xiao-shi (小式) architecture in terms of heavy woodwork method (vol. 1~27) is redefined, from which a new organization is deduced.

• Nutrition Research and Practice
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• v.13 no.5
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• pp.425-433
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• 2019

BACKGROUND/OBJECTIVES: The International Agency for Research on Cancer defined alcohol beverages and acetaldehyde derived from alcoholic beverages as a Group 1 carcinogen to humans. However, the association between alcohol consumption and gastric cancer risk has been controversial in Korean. We assessed the relationship between alcohol consumption and gastric cancer risk in Korea through a case-control study. SUBJECTS/METHODS: From 2 hospitals, a total of 316 cases with gastric cancer (208 men, 108 women) were selected and matched to 316 controls by sex and age (${\pm}5years$) during the same duration. The current status, frequency, and amount of alcohol consumption for a year three years ago were assessed by trained interviewers. RESULTS: Alcohol consumption status and frequency did not show any significant association with gastric cancer risk. However, high alcohol consumption (${\geq}20g/day$ for women or ${\geq}40g/day$ for men) significantly increased the risk of gastric cancer (odds ratio (OR) 1.73; 95% confidence interval (CI) 1.05-2.85). Gastric cancer risk was strongly positively associated with alcohol consumption of ${\geq}20g/day$, especially in women (OR 5.62; 95% CI 1.32-23.81). CONCLUSION: The results from this study suggest that excessive alcohol consumption rather than the current status or frequency of alcohol consumption contributes to the increased risk of gastric cancer, especially in women.

• Journal of Digital Convergence
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• v.17 no.4
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• pp.255-260
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• 2019

This study is about an efficient synthesis of $360^{\circ}$ video and 3D graphics. First, the video image filmed by a binocular integral type $360^{\circ}$ camera was stitched, and location values of the camera and objects were extracted. And the data of extracted location values were moved to the 3D program to create 3D objects, and the methods for natural compositing was researched. As a result, as the method for natural compositing of $360^{\circ}$ video image and 3D graphics, rendering factors and rendering method were derived. First, as for rendering factors, there were 3D objects' location and quality of material, lighting and shadow. Second, as for rendering method, actual video based rendering method's necessity was found. Providing the method for natural compositing of $360^{\circ}$ video image and 3D graphics through this study process and results is expected to be helpful for research and production of $360^{\circ}$ video image and VR video contents.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.43 no.4
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• pp.161-167
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• 2020

In the process of cutting large aircraft parts, the tool may be abnormally worn or damaged due to various factors such as mechanical vibration, disturbances such as chips, and physical properties of the workpiece, which may result in deterioration of the surface quality of the workpiece. Because workpieces used for large aircrafts parts are expensive and require strict processing quality, a maintenance plan is required to minimize the deterioration of the workpiece quality that can be caused by unexpected abnormalities of the tool and take maintenance measures at an earlier stage that does not adversely affect the machining. In this paper, we propose a method to indirectly monitor the tool condition that can affect the machining quality of large aircraft parts through real-time monitoring of the current signal applied to the spindle motor during machining by comparing whether the monitored current shows an abnormal pattern during actual machining by using this as a reference pattern. First, 30 types of tools are used for machining large aircraft parts, and three tools with relatively frequent breakages among these tools were selected as monitoring targets by reflecting the opinions of processing experts in the field. Second, when creating the CNC machining program, the M code, which is a CNC auxiliary function, is inserted at the starting and ending positions of the tool to be monitored using the editing tool, so that monitoring start and end times can be notified. Third, the monitoring program was run with the M code signal notified from the CNC controller by using the DAQ (Data Acquisition) device, and the machine learning algorithms for detecting abnormality of the current signal received in real time could be used to determine whether there was an abnormality. Fourth, through the implementation of the prototype system, the feasibility of the method proposed in this paper was shown and verified through an actual example.

• The Journal of the Convergence on Culture Technology
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• v.7 no.1
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• pp.588-594
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• 2021

Due to the recent proliferation of video contents, previous contents expressed as the character or the picture are being replaced to video and growth of video contents is being boosted because of emerging new platforms. As this accelerated growth has a great impact on the process of universalization of technology for ordinary people, video production and editing technologies that were classified as expert's areas can be easily accessed and used from ordinary people. Due to the development of these technologies, tasks like that recording and adjusting that depends on human's manual involvement could be automated through object tracking technology. Also, the process for situating the object in the center of the screen after finding the object to record could have been automated. Because the task of setting the object to be tracked is still remaining as human's responsibility, the delay or mistake can be made in the process of setting the object which has to be tracked through a human. Therefore, we propose a novel object tracking technique of CMT combining the face detection technique utilizing Haar cascade classifier. The proposed system can be applied to an effective and robust image tracking system for continuous object tracking on the smartphone in real time.