• Title/Summary/Keyword: Early hybrid

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The Effect of the Timing and Dose of Human Chorionic Gonadotropin on Oocyte Recovery, in Vitro Fertilization, and Preimplantation Development in Superovulation of Mouse (생쥐에서 과배란 유도시 인간융묘 성선자극 홀몬 투여 방법이 체외수정 및 배자의 체외성장에 미치는 영향에 관한 연구)

  • Yang, S.H.;Kim, H.M.;Oh, S.H.;Son, Y.S.;Yoo, H.K.;Woo, B.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.2
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    • pp.165-176
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    • 1994
  • This study was carried out to investigate the effect of the timing and dose of human chorionic gonadotropin(hCG) on oocyte recovery, in vitro fertilization, and preimplantation development in superovulation of mouse. F1 hybrid($C57BL{\times}CBA$) mice were obtained and superovulation was induced in female mice by sequential intraperitoneal injection of PMSG and hCG. In the first series of experiments, mice received 5 IU of PMSG given intraperitoneally, and 48 hours later were injected 1 IU, 5 IU, or 10 IU of hCG respectively. In the second series of experiments, mice received 5 IU of PMSG given intraperitoneally and were injected 5IU of hCG 36, 48, or 60 hours later respectively. 1. When the mice received 5 IU of PMSG given intraperitoneally and 48 hours later were injected 1 ItT, 5 IU, or 10 IU of hCG respectively, there were no differences in the total number of the oocytes obtained from the three experimental groups. When the cultures were examined 48 hrs after the termination of insemination the proportion of unfragmented oocytes which had developed over two-cell stage was observer to be lowest in 10 IU hCG group. When the cultrues were examined 120 hour after termination of insemination the proportion of embryos which had developed to the blastocyst stage was observed to be significantly higher in 10IU hCG group than 5IU hCG group(p<0.05), but there was no difference between 10 IU hCG group and 1IU hCG group. 2. When the mice received 5 IU of PMSG and were injected 5 IU of hCG 36, 48, or 60 hours later respectively, there were no differences in the total number of oocytes obtained from the three experimental groups. When cultures were examined 48 hour after the termination of insemination the proportion of unfragmented oocytes which had developed over two-cell stage was observed to be significantly lower in 36 hour interval group than 48 hour interval and 60 hour interval group(p<0.05). When the cultures were examined 120 hour after termination of insemination the proportion of embryos which had developed to the blastocyst stage was found to be higher in 60 hour interval group than 36 interval or 48 hour interval group (P<0.05), and the proportion of hatching blastocyst was found to be higher in 60 hour interval group as well. In this study, it was concluded that the administration of adequate dose of hCG, and long (60 hour) PMSG-hCG interval were necessary in superovulation of mice($C57BL{\times}CBA$) in order to get a large number of oocytes which had an early oocytes which had an early embryonic developmental capability when fertilized in vitro, and especially it had better have been avoided to administer a large dose of hCG.

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Current status and prospects of molecular marker development for systematic breeding program in citrus (감귤 분자육종을 위한 분자표지 개발 현황 및 전망)

  • Kim, Ho Bang;Kim, Jae Joon;Oh, Chang Jae;Yun, Su-Hyun;Song, Kwan Jeong
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.261-271
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    • 2016
  • Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study.

COMPARISON OF POLYMERIZATION SHRINKAGE AND STRAIN STRESS OF SEVERAL COMPOSITE RESINS USING STRAIN GUAGE (스트레인 게이지를 이용한 수종의 복합레진의 중합수축 및 수축응력의 비교)

  • Kim, Young-Kwang;Yoo, Seung-Hoon;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.3
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    • pp.516-526
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    • 2004
  • Polymerization shrinkage of photoinitiation type composite resin cause several clinical problems. The purpose of this study was to evaluate the shrinkage strain stress, linear polymerization shrinkage, compressive strength and microhardness of recently developed composite resins. The composite resins were divided into four groups according to the contents of matrix and filler type. Group I : $Denfil^{TM}$(Vericom, Korea) with conventional matrix, Group II : $Charmfil^{(R)}$(Dentkist, Korea) with microfiller and nanofller mixture, Group III : $Filtek^{TM}$ Z250(3M-ESPE, USA) TEGDMA replaced by UDMA and Bis-EMA(6) in the matrix, and Group IV : $Filtek^{TM}$ Supreme(3M-ESPE, USA) using pure nanofiller. Preparation of acrylic molds were followed by filling and curing with light gun. Strain gauges were attached to each sample and the leads were connected to a strainmeter. With strainmeter shrinkage strain stress and linear polymerization shrinkage was measured for 10 minutes. The data detected at 1 minute and 10 minutes were analysed statistically with ONE-way ANOVA test. To evaluate the mechanical properties of tested materials, compressive hardness test and microhardness test were also rendered. The results can be summarized as follows : 1. Filling materials in acrylic molds showed initial temporary expansion in the early phase of polymerization. This was followed by contraction with the rapid increase in strain stress during the first 1 minute and gradually decreased during post-gel shrinkage phase. After 1 minute, there's no statistical differences of strain stress between groups. The highest strain stress was found in group IV and followed by group III, I, II at 10 minutes-measurement(p>.05). In regression analysis of strain stress, group III showed minimal inclination and followed by group II, I, IV during 1 minute. 2. In linear polymerization shrinkage test, the composite resins in every group showed initial increase of shrinkage velocity during the first 1 minute, followed by gradually decrease of shrinkage velocity. After 1 minute, group IV and group III showed statistical difference(p<.05). After 10 minutes, there were statistical differences between group IV and group I, III(p<.05) and between group II and group III(p<.05). In regression analysis of linear polymerization shrinkage, group II showed minimal inclination and followed by group IV, III, I during 1 minute. 3. In compressive strength test, group III showed the highest strength and followed by group II, IV, I. There were statistical differences between group III and group IV, I(p<.05). 4. In microhardness test, upper surfaces showed higher value than lower surfaces in every group(p<.05).

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Status of Maize Production and Distribution in South East Asia (동남아시아 옥수수 생산 및 유통현황)

  • Lee, Sang-Kyu;Song, Jun-Ho;Baek, Seong-Bum;Kwon, Young-Up;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.3
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    • pp.318-332
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    • 2015
  • The maize production in South-eastern Asian countries showed a continuous increase with increasing poultry-livestock from the beginning of the 1990s to early 2010. Also the need for a new variety development of each contries was increased rapidly in the same period. Single-Cross hybrid varieties have been developed and supplied from 2001 instead of multi-cross maize varieties since 1992 in Indonesia. In Cambodia, CP group is mainly manufacturing feeds with most of the forage maize from farmers who are growing its seeds from the company. Cambodian main cultivars are varieties of multinational corporations such as DK8868 from Monsanto, NK6326, NK7328 from Syngenta and CP333 from CP group including local business company. Vietnam is the main maze importing country in South-Eastern Asia which had imported 13 times scale of amount compared to exports in average from 1990 to 2011. Vietnamese government has developed a range of varieties for improving their efficiency in production, such as the LVN-10 with political investments. Their production has been reached to 80% of the total. According to the 2012 MIFAFF (Ministry for Food, Agriculture, Forestry and Fisheries) data in Korea, domestic edible maize cultivation area was approximately 15,000ha. It showed 74,399 tons of production, 3.8% of food self-sufficiency in maize and around 0.9% of grain self-sufficiency rate. The consumption of grain is mostly rely on imports in Korea. To overcome the limit of the domestic seed market and increase maize self-sufficiency, the need to develop maze varieties for world-class is increasing at present through analyzing the market trend and prospect of the seed industry in South-eastern Asia.

Optimum Application Level of Dicamba Heribicide for Control of Abutilon avicennae in Silage Corn Field (사일리지용 옥수수 포장에서 어저귀 (Abutilon avicennae) 방제를 위한 Dicamba 액제 적정 시용수준 구명)

  • Seo, S.;Chung, E.S.;Kim, J.G.;Kang, W.S.;Choi, K.J.;Rim, Y.W.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.1
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    • pp.75-80
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    • 1999
  • A field experiment was carried out to determine the optimum application level of dicamba herbicide for efficient control of Abutilon avicennae in silage corn field. Five treatments of application levels (0 ; control, $0.75{\ell},\;1.0{\ell},\;1.25{\ell}\;and\;1.5{\ell}$) were arranged at National Livestock Research Institute, RDA, Suwon in 1995 and 1996. The hybrid silage corn was P 3352 and dicamba herbicide was applied at 5~6 leaf stage of corn. The growth of corn was poor in control plot, and the days for tasseling and silking were delayed 2~3 days when compared with the plots of herbicide application. The control efficiency of Abutilon avicennae was excellent as 94.7~98.1% in the plots of dicamba application. A slight injury of herbicide was observed at early stage of corn when applied at $1.0{\ell},\;1.25{\ell}\;and\;1.5{\ell}$ of dicamba per ha. Forage dry matter(DM) yield, length and weight of ear in the plots of dicamba treatments were significantly higher than those of control. The DM yield of control(12,070kg/ha) was only 64.2~66.9% of dicamba treatment plot(18,050~18,795kg/ha). However, there was no yield difference among dicamba application level. The percentage of ear to total DM yield was ranged from 53.4 to 56.8%. The nutritive value(crude protein, ADF, NDF, digestibility and TDN) was very similar among all treatments. From the above results, it is recommended that optimum application level of dicamba herbicide was $0.75{\sim}1.0{\ell}/ha$ for efficient control of Abutilon avicennae and for silage corn production without herbicide injury.

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Effects of Polyethylene Mulch and Levels and Placements of Nitrogen on Soil Properties and Sweet Corn Growth (비닐 피복, 질소시비량 및 시비방법이 토양의 이화학적 특성과 단옥수수의 생육에 미치는 영향)

  • 이석순;백준호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.334-339
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    • 1985
  • A sweet corn hybrid, Honey Bantam, was planted on 24 May, 1984 in a silty clay loam soil to investigate the effects of polyethylene(P.E.) mulch and different levels and placements of Nitrogen(N) on soil properties and sweet corn growth. A split-split plot design with three replications was employed; P.E. mulch and bare soil were main plot, N levele of 8, 12, 16, and 20 kg/10a were subplot, and band and broadcast of fertilizers were sub-subplots. At early growth stage soil temperature under P.E. mulch was higher than that in bare soil by 5-10$^{\circ}C$, but the differences decreased as plant growth advanced. Soil hardness increased with soil depth while P.E. mulch reduced soil hardness probably by holding high soil moisture. Soil pH decreased up to the 6th week after planting and then increased in bare soil, but it contineously decreased up to the 8th weeks under P.E. mulch regardless N levels and placements. Electrical conductivity(EC) of soil increased up to the 6th weeks after planting and then decreased in all treatments except broadcast of fertilizers under P.E. mulch where EC increased contineously. Generally, soil EC under P.E. mulch was higher than that in broadcast. Broadcast of fertilizers did not affect emergence of seedlings in all N levels under P.E. mulch and bare soil, but band of fertilizers at all N levels under P.E. mulch and higher levels of N in bare soil reduced emergence rate significantly. Percent stand was possitively correlated with soil EC and it strongly influenced the number of marketable ears. Plant growth was enhanced and silking date was earlier by 14-19 days under P.E. mulch compared to bare soil probably due to increased soil moisture, reduced soil hardness and higher soil temperature.

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An Approach for Enhancing Current Korean e-Grocery Business Focusing on Delivery Service Alternatives (한국의 e-Grocery 배송서비스 대안에 관한 연구)

  • Koo, Jong-Soon;Lee, Jung-Sun;Jeon, Dong-Hwa
    • International Commerce and Information Review
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    • v.13 no.3
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    • pp.169-201
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    • 2011
  • There was a new wave in grocery business with development of information and technology, thus a movement from traditional stores to online stores, In order to expand the scale of traditional supermarket and to satisfy the customers' needs, they provide offline and online services simultaneously. This paper is based on the previous studies which had been researched in developed countries from late 1990s to early 2000s and the purpose of this study is to introduce the idea and operation system of e-Grocery business. Moreover, we suggest the alternatives on delivery service methods in order to satisfy the customers' needs through analyzing the current condition of e-Grocers in Korea. According to the result of this study, Korean e-Grocers offer only attended home delivery services. In our opinion, Korean supermarkets have to take hybrid model which Tesco.com is using. There are some alternatives to increase the profits of Korean e-Grocers and to provide better services to their customers as follows: As an alternatives for delivery services, picking service is the easiest and cheapest way to apply for supermarkets. This is very useful for working women and also it is possible to order by smartphone recently. They can order the goods to the closest local supermarkets from working place, and then they pick them up on the way home from working off. In order to improve the quality of delivery services, to use the reception box will be the way to provide better services to the customers. The reception box is a way to protect the quality of goods such as fresh-cut product, which require the freshness through the temperature adjustment, and also to keep the safety of ordered goods through locking system Through this system, supermarkets are able to use attended or unattended services under the customers' satisfaction. However, using the reception box is expensive, so shared reception box will be an alternative. As an alternative for development of e-Grocery business, the advertisement for e-Grocery business has to be supported in order to attract potential customers in e-Grocery business. Furthermore, the main concerns of e-Grocery business such as the sanitation and safety of goods, and convenience must be guaranteed in order to keep the loyal customers and to attract new customers.

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Development of Landscape Urbanism in Practice (랜드스케이프 어바니즘의 실천적 전개 양상)

  • Kim, Youngmin;Jeong, Wookju
    • Journal of the Korean Institute of Landscape Architecture
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    • v.42 no.1
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    • pp.1-17
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    • 2014
  • The Practice of Landscape Urbanism has been criticized on two aspects; Landscape Urbanism does not yet provide differentiated forms of planning and design neither able to fill the gap between theory and practice. In response to these criticisms, Landscape Urbanists have tried to provide additional cases that can exemplify achievements of Landscape Urbanism in practice. There has been another type of effort to suggest distinct planning and design strategies of Landscape Urbanism from theoretical texts. However, these approaches overlooked the fact that professional practice actually gave huge influence on the theoretical framework of Landscape Urbanism. Based on this observation, the study examined the development process of Landscape Urbanism by verifying the correlation between its practice and theory. Through an extensive literature review, the study suggested horizontality, infrastructure, process, ecology, media, hybrid, site, and scale as eight major concepts of Landscape Urbanism. Afterwards, the study classified 51 projects cited in major essays and articles of Landscape Urbanism into four categories: Archetypal projects, precedent projects, exemplary projects, and progressive projects. The correlation between strategies of the selected projects and main theoretical concepts was analyzed. The study found out that the early stage of the theory was focused on defining and explaining the new design and planning approaches of contemporary design projects related with landscape. However, these days, Landscape Urbanism became a more productive in providing diverse types of practice sharing the direction and vision proposed by the theory. Various projects influenced in constructing theoretical structure of Landscape Urbanism as well as proved that the suggestions of Landscape Urbanism could be effective to reorganize contemporary cites in the form of design and planning strategies. The observation of this study can contribute to provide proper answers to the criticism on practice of Landscape Urbanism and be helpful in understanding the limits and unrealized potentials of Landscape Urbanism as a practical theory.

Rethinking Korean Women's Art from a Post-territorial Perspective: Focusing on Korean-Japanese third generation women artists' experience of diaspora and an interpretation of their work (탈영토적 시각에서 볼 수 있는 한국여성미술의 비평적 가능성 : 재일동포3세 여성화가의 '디아스포라'의 경험과 작품해석을 중심으로)

  • Suh, Heejung
    • The Journal of Art Theory & Practice
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    • no.14
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    • pp.125-158
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    • 2012
  • After liberation from Japanese colonial rule in 1945, there was the three-year period of United States Army Military Government in Korea. In 1948, Democratic People's Republic of Korea, and Republic of Korea were established in the north and south of the Korean Peninsula. The Republic of Korea is now a modern state set in the southern part of the Korean. We usually refer to Koreans as people who belong to the Republic of Korea. Can we say that is true exactly? Why make of this an obsolete question? The period from 1945 when Korea was emancipated from Japanese colonial rule to 1948 when the Republic of Korea was established has not been a focus of modern Korean history. This three years remains empty in Korean history and makes the concept of 'Korean' we usually consider ambiguous, and prompts careful attention to the silence of 'some Koreans' forced to live against their will in the blurred boundaries between nation and people. This dissertation regards 'Koreans' who came to live in the border of nations, especially 'Korean-Japanese third generation women artists'who are marginalized both Japan and Korea. It questions the category of 'Korean women's art' that has so far been considered, based on the concept of territory, and presents a new perspective for viewing 'Korean women's art'. Almost no study on Korean-Japanese women's art has been conducted, based on research on Korean diaspora, and no systematic historical records exist. Even data-collection is limited due to the political situation of South and North in confrontation. Representation of the Mother Country on the Artworks by First and Second-Generation Korean-Japanese(Zainich) Women Artists after Liberation since 1945 was published in 2011 is the only dissertation in which Korean-Japanese women artists, and early artistic activities. That research is based on press releases and interviews obtained through Japan. This thesis concentrates on the world of Korean-Japanese third generation women artists such as Kim Jung-sook, Kim Ae-soon, and Han Sung-nam, permanent residents in Japan who still have Korean nationality. The three Korean-Japanese third generation women artists whose art world is reviewed in this thesis would like to reveal their voices as minorities in Japan and Korea, resisting power and the universal concepts of nation, people and identity. Questioning the general notions of 'Korean women' and 'Korean women's art'considered within the Korean Peninsula, they explore their identity as Korean women outside the Korean territory from a post-territorial perspective and have a new understanding of the minority's diversity and difference through their eyes as marginal women living outside the mainstream of Korean and Japanese society. This is associated with recent post-colonial critical viewpoints reconsidering myths of universalism and transcendental aesthetic measures. In the 1980s and 1990s art museums and galleries in New York tried a critical shift in aesthetic discourse on contemporary art history, analyzed how power relationships among such elements as gender, sexuality, race, nationalism. Ghost of Ethnicity: Rethinking Art Discourses of the 1940s and 1980s by Lisa Bloom is an obvious presentation about the post-colonial discourse. Lisa Bloom rethinks the diversity of race, ethnicity, sexuality, and gender each artist and critic has, she began a new discussion on artists who were anti-establishment artists alienated by mainstream society. As migration rapidly increased through globalism lead by the United States the aspects of diaspora experience emerges as critical issues in interpreting contemporary culture. As a new concept of art with hybrid cultural backgrounds exists, each artist's cultural identity and specificity should be viewed and interpreted in a sociopolitical context. A criticism started considering the distinct characteristics of each individual's historical experience and cultural identity, and paying attention to experience of the third world artist, especially women artists, confronting the power of modernist discourses from a perspective of the white male subject. Considering recent international contemporary art, the Korean-Japanese third generation women artists who clarify their cultural identity as minority living in the border between Korea and Japan may present a new direction for contemporary Korean art. Their art world derives from their diaspora experience on colonial trauma historically. Their works made us to see that it is also associated with postcolonial critical perspective in the recent contemporary art stream. And it reminds us of rethinking the diversity of the minority living outside mainstream society. Thus, this should be considered as one of the features in the context of Korean women's art.

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Optimum Application Level of Dicamba Herbicide for Control of Calystegia japonica in Silage Corn Field (사일리지용 옥수수 포장에서 메꽃 (Calystegia, japonica) 방제를 위한 Dicamba 액제 적정 시용수준 구명)

  • Seo, S.;Chung, E.S.;Kim, J.G.;Kang, W.S.;Kim, W.H.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.3
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    • pp.259-264
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    • 1999
  • A field experiment was carried out to determine the optimum application level of dicamba herbicide for efficient control of Calystegia japonica in silage corn field. Six treatments of application levels (0 ; control, $0.75{\ell},\;1.0{\ell},\;1.25{\ell},\;1.5{\ell},\;and\;2.0{\ell}/ha$) were arranged at National Livestock Research Institute, RDA, Suwon in 1995. The hybrid silage corn was DK 729, and dicamba herbicide was applied at 5~6 leaf stage of corn. The growth of corn was poor in control plot, and the days for tasseling and silking were delayed 1~4 days when compared with the plots of herbicide application. The control efficiency of Calystegia japonica was excellent as 74.3~94.6% in the plots of dicamba application. A slight injury of herbicide was observed at early stage of corn when applied at $1.25{\ell}$ and $1.5{\ell}$ of dicamba per ha, and severe injury was found at $2.0{\ell}$ of dicamba. Forage dry matter(DM) yield, and length of ear in the plots of dicamba treatments were significantly higher than those of control. The DM yield of control(4,866kg/ha) was only about 30% of dicamba treatment plot (14,960~16,340kg/ha). However, there was no yield difference among dicamba application level. The percentage of ear to total DM yield was ranged from 33.6 to 39.4%. With application of herbicide, the contents of crude protein and nitrogen free extract were increased, and that of crude fiber was decreased in stover. However, nutritive value of corn ear was very similar among all treatments, regardless of herbicide treatment. From the above results, it is recommended that optimum application level of dicamba herbicide was $0.75{\sim}1.0{\ell}/ha$ for efficient control of Calystegia japonica and for silage corn production without herbicide injury.

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