• International Journal of Industrial Entomology and Biomaterials
• /
• v.8 no.2
• /
• pp.145-149
• /
• 2004

The expression of a fusion protein comprised of the B. thuringiensis crystal protein, Cry1Ac, and enhanced green fluorescent protein (EGFP) in Escherichia coli XLl-blue was examined. Three recombinant plasmids were transformed into E. coli XL1-blue and named as ProAc/Ec, MuEGFP/Ec and ProMu-EGFP/Ec, respectively. All transformants were observed by light and fluorescence microscopy at mid-log phase. The expression in E. coli transformants, ProMu-EGFP/Ec and MuEGFP/Ec, exhibited bright enough fluorescence to be observed. Furthermore, ProMu-EGFP/Ec produced fluorescent inclusions, which may have been recombinant crystals between EGFP and Cry1Ac while MuEGFP/Ec expressed soluble EGFP in cell. In SDS-PAGE, ProAc/Ec had 130 kDa crystal protein band and MuEGFP/Ec had thick 27 kDa EGFP band. However, ProMu-EGFP/Ec had about 150 kDa fusion protein band. Accordingly, these results indicated that a fusion protein between the B. thuringiensis crystal protein and a foreign protein under the lacZ promoter was successfully expressed as granular structure in E. coli. It is suggested that the E. coli expression system by N-terminal fusion of B. thuringiensis crystal protein may be useful as excellent means for fusion expression and characterization of B. thuringiensis fusion crystal protein.

• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.6
• /
• pp.555-562
• /
• 2010

For the rapid and reliable detection of toxic compounds in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. Using a synthetic stream water (EC=0.12 mS/cm and pH=7.2), the baseline steady-state EC and pH values were 0.5~1.2 mS/cm and ~2.5 over 7 days of testing at HRT 30 minutes. When nitrite compounds were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. Optimum HRT was 30 min and this HRT could be decresed by using smaller sulfur particles.

• Molecules and Cells
• /
• v.39 no.3
• /
• pp.242-249
• /
• 2016

A balance between production and degradation of reactive oxygen species (ROS) is critical for maintaining cellular homeostasis. Increased levels of ROS during oxidative stress are associated with disease conditions. Antioxidant enzymes, such as extracellular superoxide dismutase (EC-SOD), in the extracellular matrix (ECM) neutralize the toxicity of superoxide. Recent studies have emphasized the importance of EC-SOD in protecting the brain, lungs, and other tissues from oxidative stress. Therefore, EC-SOD would be an excellent therapeutic drug for treatment of diseases caused by oxidative stress. We cloned both the full length (residues 1-240) and truncated (residues 19-240) forms of human EC-SOD (hEC-SOD) into the donor plasmid pFastBacHTb. After transposition, the bacmid was transfected into the Sf9-baculovirus expression system and the expressed hEC-SOD purified using FLAG-tag. Western blot analysis revealed that hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), as detected by the FLAG antibody. A water-soluble tetrazolium (WST-1) assay showed that both full length and truncated hEC-SOD proteins were enzymatically active. We showed that a potent superoxide dismutase inhibitor, diethyldithiocarbamate (DDC), inhibits hEC-SOD activity.

• Journal of Environmental Science International
• /
• v.16 no.4
• /
• pp.441-447
• /
• 2007

This study was carried out to investigate the effect of electrochemical (EC) disinfection of artificial wastewater contaminated by Escherichia coli culture. Circulated batch type electrochemical disinfection system using three plates electrodes was used. Also, the several factors (pH, ORP, DO, temperature, current, conductivity) were measured in order to investigate the fundamental design factor in the EC disinfection system. It was demonstrated that the EC process was highly effective for wastewater disinfection. At the constant voltage, the disinfection efficiency was increased according to time. The disinfection efficiency and current increased as the increase of voltage. The variation of conductivity was a little related to the variation of CFU (colony forming units). The differences in disinfection efficiency according to the ice pack and the variation of electrodes were not occurred. The EC disinfection efficiency and current increased according to the increase of circulating flow rate.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2019.07a
• /
• pp.243-244
• /
• 2019

본 논문에서는지속 가능한 IoT Platform을 개발 하였다. 개발된 IoT(Internet of Things) Platform은 센서를 제어하는 특정 시스템과의 통신을 통한 제어 및 데이터 전달에 용이하고, 제한된 통신 환경 및 낮은 전력에서도 지속적인 작동이 가능하여 가용성(Availability)과 확장성(Extensibility)이 뛰어나다. 본 논문에서는 지속 가능한 IoT Platform의 테스트를 위해 클라우드 컴퓨팅 플랫폼인 AWS EC2(Amazon Elastic Compute Cloud, EC2)에 구축하였으며, DataBase 서버로는 오픈 소스 관계형 데이터베이스 관리 시스템인 MariaDB를 선정하였으며, 센서를 제어하는 특정 시스템인 스마트 미러 시스템(Smart Mirror System)과 미세먼지 제어 시스템(Air Quality Control System)에 기존의 Google IoT Platform에서 사용되는 MQTT Protocol(Message Queuing Telemetry Transport Protocol)와 지속 가능한 IoT Platform를 위해 개발된 TCP/IP Protocol를 사용하여 비교했다. 개발된 IoT Platform은 UTM(Unmanned Aircraft System Traffic Management)으로 확장할 계획이다.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2005.04a
• /
• pp.296-299
• /
• 2005

Time series of spring discharge data in Jeju island can provide abundant information on the spatial groundwater system. In this study, the classification based on time series of spring discharge was performed with clustering analysis: discharge rate and EC. Peak discharges are mainly observed in august or september. However, double peaks and late peaks of discharge are also observed at a plenty of springs. Based on results of clustering analysis, it can be deduced that GH model is not appropriate for the conceptual model of Groundwater system in Jeju island. EC distributions in dry season are also support the conclusion.

• The Journal of Society for e-Business Studies
• /
• v.4 no.3
• /
• pp.95-118
• /
• 1999

There have been many research papers and practical implementations on the various EC(Electronic Commerce) aspects such as merchant system, security, payment gateway, legal and policy issues. However, it is very hard to find the systematic approaches and business process models from the viewpoint of the company willing to start B2C(Business-To-Customer) EC. Therefore, we develop business process models for the planning, systems analysis and design, implementation, and operation of internet shopping mall to suggest a guideline and reference model for the realization of successful B2C EC.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.15 no.6
• /
• pp.170-176
• /
• 2007

It is time to prepare the phaseout of R134a, the current refrigerant, in automotive air conditioning system because the EC deadline has been coming with new platform vehicles in 2011 and all vehicles by 2017. Until now a high-pressure carbon dioxide($CO_2$) system is the leading replacement of R134a in European auto-makers but there is no firm agreement in the world automotive market. Recently three new fluids have been announced as the possibilities from Honeywell, DuPont and INEOS Fluor. The new alternative refrigerant should meet the requirements like non flammable, non toxic, no ozone depletion effect and low GWP(under 150 to meet EC regulation). The objectives of this paper are to review the fluid H from Honeywell, the more possible alternative refrigerant in 3 new fluids, compare the properties of R134a & fluid H and see the possibility as a replacement of R134a. In this experimental paper we ran and reviewed the cooling performance data in the bench system, the vehicle and the field test. We found the possibility of fluid H system to meet the R134a system performance with some hardware modifications but agreed that it is still needed to study about the long term safety, environmental effects, material compatibilities and so on.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.9 no.5
• /
• pp.50-59
• /
• 2006

This study was initiated to investigate soil chemical properties under different soil systems. Data such as soil acidity(pH), electrical conductivity(EC), organic matter content(OMC), and cation exchange capacity(CEC) were analyzed with samples from multi-layer, USGA, and mono-layer systems. N, P, K and micronutrients were also measured. Multi-layer system was built up to 60-cm depth with rootzone layer, intermediate layer and two drainage layers. USGA system 45 centimeters deep was constructed with rootzone layer, intermediate layer and drainage layer. Mono-layer system, however, was made only with a 30-cm rootzone layer. Differences were observed in soil pH, EC, OMC, CEC and micronutrients. Soil pH was acceptable for turfgrass growth a year after establishment, being 5.5 to 6.5 in the study. Differences were greatly observed for EC among soil systems. Values of EC for multi-layer, USGA, and mono-layer systems were 39.79, 31.26 and 103.54 uS/em, respectively. The increase rate was approximately 4 to 8 times greater with mono-layer system than those with other two systems. Therefore, it was necessary to avoid micronutrient deficiency such as Fe, Mn etc. through an effective management program in mono-layer system because of its faster potential feasibility of salt accumulation. The greatest OMC was associated with USGA system, being 0.97% which was 11% over that of the other systems. Slight differences were observed for CEC among them. Mono-layer system produced 1.45 me/100g, 10.3% and 8.9% lower in CEC than those of multi-layer and USGA system, respectively. Micronutrients such as Fe, Zn, and Mn etc. were below the level required for turf growth, regardless of soil systems. It was considered that one year after turf establishment was not enough to build up micronutrients in sand-based soil systems to the normal level for a turf growth. These results demonstrate that intensive management program including grow-in concept fertilization should be integrated into sand-based soil systems, even after a year in establishment. Regular nutrient monitoring by soil analyses is a strong necessity to decide the kinds and amount of fertilizer. Also, strategic management program must be selectively employed according to sports turf soil systems.

• Journal of Korean Society of Environmental Engineers
• /
• v.34 no.12
• /
• pp.828-834
• /
• 2012

The ecotoxicity detection device for preliminary test (Test jig) was manufactured to develop the biological early warning system using Vibrio fischeri. In this study, the ecotoxicity detection charateristics of the Test jig was investigated for 6 heavy metals (Cr, Zn, Pb, Cd, Cu and Hg). It was observed that relative luminescence unit (RLU) of Vibrio fischeri constantly decreased by the concentrations of the tested heavy metals. In contrast with other heavy metals, RLUs of Pb and Hg constantly decreased even at low concentrations. RLU of Hg drastically decreased when its concentration increased from 0.13 mg/L to 0.25 mg/L. $EC_{50}$ values of Cr, Zn, Pb and Cd gradually decreased with exposure time, whereas there was no significant change in $EC_{50}$ values of Cu and Hg with time. On the other hand, $EC_{50}$ values between the Test jig and Reference device were compared to evaluate the ecotoxicity detection performance of the Test jig. No big difference was found in $EC_{50}$ vlaues between the two devices, indicating that the Test jig could be applied as the ecotoxicity detection device for heavy metals.