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A New Methodology of Measuring Water Toxicity using Sulfur Oxidizing Bacteria  

Oh, Sang-Eun (Department of Biological Environment, Kangwon National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.32, no.6, 2010 , pp. 555-562 More about this Journal
Abstract
For the rapid and reliable detection of toxic compounds in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. Using a synthetic stream water (EC=0.12 mS/cm and pH=7.2), the baseline steady-state EC and pH values were 0.5~1.2 mS/cm and ~2.5 over 7 days of testing at HRT 30 minutes. When nitrite compounds were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. Optimum HRT was 30 min and this HRT could be decresed by using smaller sulfur particles.
Keywords
Biosensor; Toxicity; Electrical conductivity; Sulfur oxidizing bacteria; Sulfur particles;
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1 Palma, P., Palma, V. L., Matos, C., Fernandes, R. M., Bohn, A., Soares, A.M.V.M., and Barbosa, I. R., "Effects of atrazine and endosulfan sulphate on the ecdysteroid system of Daphniamagna," Chemosphere, 74, 676-681(2009).   DOI   ScienceOn
2 Liu, Y., Qin, Z., Wu, X., and Jiang, H., "Immune-biosensor for aflatoxin B1 based bio-electrocatalytic reaction on micro-comb electrode," Biochemi. Eng. J., 32, 211-217 (2006).   DOI   ScienceOn
3 Tront, J. M., Fortner, J. D., Pl?ze, M., Hughes, J. B., and Puzrin, A. M., "Microbial fuel cell biosensor for in situ assessment of microbial activity," Biosens. Bioelectron., 24, 586-590 (2008).   DOI   ScienceOn
4 Lake Access Home Page, http://lakeaccess.org/russ/conductivity.htm(2010).
5 Zlatev, R., Magnin, J.-P., Ozil, P., and Stoytcheva, M., "Bacterial sensors based on Acidithiobacillus ferrooxidans: Part I. $Fe^{2+}$ and $S_2{O_3}^{2-}$ determination," Biosens. Bioelectron., 21, 1493-1500 (2006).   DOI   ScienceOn
6 Madigan, M. T., Martinko, J. M., and Parker, J., Brock biology of microorganisms, 10th ed., Pearson Education Inc., New York(2003).
7 Liu, H. and Logan, B. E., "Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane," Environ. Sci. Technol. 38(14), 4040-4046(2004).
8 Eaton, J. G., "Chronic toxicity of a copper, cadmium and zinc mixture to the fathead minnow (Pimephales promelas rafinesque)," Water Res., 7, 1723-1736(1973).   DOI   ScienceOn
9 Hsieh, C.-Y., Tsai, M.-H., Ryan, D.K., and Pancorbo, O. C., "Toxicity of the 13 priority pollutant metals to Vibriofisheri in the Microtox? chronic toxicity test," Sci. Total Environ, 320, 37-50(2004).   DOI   ScienceOn
10 Tzoris, A., Fernandez-Perez, V., and Hall, E. A. H., "Direct toxicity assessment with a mini portable respirometer," Sens. Actuators, B: Chemical, 105, 39-49(2005).   DOI
11 Gu, M. B., and Gil, G. C., "A multi-channel continuous toxicity monitoring system using recombinant bioluminescent bacteria for classification of toxicity," Biosens. Bioelectron., 16, 661-666(2001).   DOI   ScienceOn
12 Kong, Z., Vanrolleghem, P. A., and Verstraete, W., "An activated sludge-based biosensor for rapid IC50 estimation and on-line toxicity monitoring," Biosens. Bioelectron., 8, 49-58 (1993).   DOI   ScienceOn
13 Steinberg, S. M., Poziomek, E. J., Engelmann, W. H., and Rogers, K. R., "A review of environmental applications of bioluminescence measurements," Chemosphere, 30, 2155-2197 (1995).   DOI   ScienceOn
14 Inui, T., Tanaka, Y., Okayasu, Y., and Tanaka, H., "Application of toxicity monitor using nitrifying bacteria biosensor to sewerage systems," Water Sci. Technol, 45(4-5), 271-278(2002).
15 남선화, 양창용, 안윤주, 이재관. "국내 생물종을 이용한 생태독성평가 기반연구 : (I) 어류," Korean J. Limnol., 40(2), 173-183(2007).   과학기술학회마을
16 Rogers, K. R., Wiliams, L. R., "Biosensors for environmental monitoring: a regulatory perspective," Trends in Analytical Chemistry, 14(7) 289-294(1995).
17 Eilersen, A.M., Arvin, E., and Henze, M., "Monitoring toxicity of industrial wastewater and specific chemicals to a green alga, nitrifying bacteria and an aquatic bacterium," Water Sci. Technol., 50(6), 277-283(2004).
18 환경부. "물환경관리 기본계획 4대강 대권역 수질보전 기본계획," ('06-'15)(2006).
19 Hernando, M. D., Fernandez-Alba, A. R., Tauler, R., Barcelo, D., "Toxicity assays applied to wastewater treatment," Talanta, 65(3), 58-366(2005).