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Multi-component kinetics for the growth of the cyanobacterium Synechocystis sp. PCC6803

  • Kim, Hyun-Woo;Park, Seongjun;Rittmann, Bruce E.
    • Environmental Engineering Research
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    • v.20 no.4
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    • pp.347-355
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    • 2015
  • The growth kinetics of phototrophic microorganisms can be controlled by the light irradiance, the concentration of an inorganic nutrient, or both. A multi-component kinetic model is proposed and tested in novel batch experiments that allow the kinetic parameters for each factor to be estimated independently. For the cyanobacterium Synechocystis sp. PCC6803, the estimated parameters are maximum specific growth rate $({\mu}_{max})=2.8/d$, half-maximum-rate light irradiance $(K_L)=11W/m^2$, half-inhibition-rate light irradiance $(K_{L,I})=39W/m^2$, and half-maximum-rate concentration for inorganic carbon $(K_{S,Ci})=0.5mgC/L$, half-maximum-rate concentration for inorganic nitrogen $(K_{S,Ni})=1.4mgN/L$, and half-maximum-rate concentration for inorganic phosphorus $(K_{S,Pi})=0.06mgP/L$. Compared to other phototrophs having ${\mu}max$ estimates, PCC6803 is a fast-growing r-strategist relying on reaction rate. Its half-maximum-rate and half-inhibition rate values identify the ranges of light irradiance and nutrient concentrations that PCC6803 needs to achieve a high specific growth rate to be a sustainable bioenergy source. To gain the advantages of its high maximum specific growth rate, PCC6803 needs to have moderate light illumination ($7-62W/m^2$ for ${\mu}_{syn}{\geq}1/d$) and relatively high nutrient concentrations: $N_i{\geq}2.3 mgN/L$, $P_i{\geq}0.1mgP/L$, and $C_i{\geq}1.0mgC/L$.

Clinical Study on 1 Case of patient with Urinary Retention (요폐환자(尿閉患者) 1례(例)에 대한 임상적(臨床的) 고찰(考察))

  • Kim, Jae-Hyeng;Cho, Chung-Sik;Kim, Chul-Jung
    • Journal of Haehwa Medicine
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    • v.14 no.1
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    • pp.43-49
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    • 2005
  • One case of patient with urinary retention was reported in this clinical study. After the study, the results were as follows: 1. Anulesis belongs to l$\acute{o}$ng-bi(癃閉) in oriental medicine and the patient of this case belongs to chronic anulesis. 2. The patient of this case was thought as broke out anuresis by vesical nerve palsy of diabetic bladder pathy and sequela of stroke, and ischuria of long lie down 3. The patient of this case changeable process is supposed to urinary frequency, 'bi(閉)', 'l$\acute{o}$ong(癃)', urinary incontinence and urinary frequency. 4. First time of drug treatment is GAMIPHALJENG-SAN(ji$\bar{a}$-w$\grave{e}$i-b$\bar{a}$-h$\bar{e}$ng-s$\check{a}$n) by oral administration that action of alleviate a fever, water utilization and metaphase, convalescence time of drug treatment is PHALMIWANGAMI (b$\bar{a}$-w$\grave{e}$i-w$\acute{a}$n-ji$\bar{a}$-w$\grave{e}$i), YIKLUENG-TANG(yi-ling-t$\bar{a}$ng), CHUKYOO-TANG(s$\grave{u}$-ni$\grave{a}$o-t$\bar{a}$ng) that action of recreation. 5. Accupuncture treatment is s$\bar{a}$n-x$\bar{i}$ng-xuw$\acute{e}$, zw$\acute{u}$-s$\bar{a}$n-li, qi-h$\check{a}$i, gu$\bar{a}$n-yu$\acute{a}$n, zh$\bar{o}$ng-ji by mild supplementing and reducing manipulation of needle and heat accupuncture is qi-h$\check{a}$i, gu$\bar{a}$n-yu$\acute{a}$n, zh$\bar{o}$ng-ji-xu$\acute{e}$. 6. The other treatment is a sitz bath that prevent of urinary tract infection, and relieve of initial acute auresis by using of urethral catheterization.

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Palm Vitamin E Prevents Osteoporosis in Orchidectomized Growing Male Rats

  • Ima-Nirwana, S.;Kiftiah, A.;Zainal, A.G.;Norazlina, M.;Gapor, M.T.;Khalid, B.A.K.
    • Natural Product Sciences
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    • v.6 no.4
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    • pp.155-160
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    • 2000
  • Testosterone deficiency increased bone resorption, giving rise to osteoporosis. Testosterone deficiency also increased lipid peroxidation and free radical formation. Free radicals have been shown to be toxic to osteoblasts as well as to activate osteoclasts. In this study, the effects of giving an antioxidant, i.e. vitamin E-rich extract from palm oil on bone mineral density and calcium content was studied. Palm vitamin E prevented the loss in bone mineral density due to orchidectomy, seen in the whole femur, proximal amd midshaft regions, as well as L4 vertebra. Similar observations were seen in bone calcium content of the L5 vertebra. Giving palm olein also prevented the loss in bone mineral density in the femoral midshaft and L4 vertebra; and bone calcium content in the L5 vertebra. In conclusion, vitamin E-rich extract from palm oil was effective in preventing the loss in bone mineral density and calcium content of orchidectomized male rats. This action is probably due to its role as an antioxidant.

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A Quantitative Method for Estimating Damages in Fishery Production due to Artificial Environmental Deterioration in the Tidal Flat Fishing Grounds (천해어장에서 인위적 환경훼손에 의한 어업생산 감소량 추정방법)

  • PARK Joo Seok;KANG Yong Joo;ZHANG Chang Ik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.4
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    • pp.402-408
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    • 2003
  • A quantitative method was suggested for estimating damages in fishery production due to the diffusion and deposition of suspended silt and clay by various construction processes in tidal flat fishing grounds. Marine populations are maintained through the process of spawning, growth, recruitment, natural death and death by fishing each year. All of the year classes of the population in a fishery ground could be affected when damages occur by human activities such as land filling or reclamation. The propose of this study is to calculate damages in terms of fishery production using a quantitative population dynamic method. If the maximum age in the population is $X_\lambda,$ the starting year of damage is $t_s,$ and the ending year of damage is $t_e,$ the number of year classes damaged is $t_{s-n\lambda}-t_e,$ Many year classes present in the year $t_s,$ and so if damages occur, they Influence all the year classes which are present in the population. Damaged year classes in year $t_e$ would still be in the population until the year $t_{e+n\lambda}$, where $n_{\lambda}$ is the oldest age class. If the expected yield of a year class is constant, the total yield from year classes in the fishing ground during the construction periods can be calculated as follows: $Y_\Phi=[(t_e-t_s+1)+n_c]{\cdot}Y_E+\sum\limits^{n_\lambda-n_c}_{l=1}\;\sum\limits^{n_\lambda-n_c}_{l=i}\;Y_{n_c+i}$ This method was applied for damage estimation in the production of Ruditapes philippinarum in a tidal flat fishing ground.

Chitosan Silver Nano Composites (CAgNCs) as Antibacterial Agent Against Fish Pathogenic Edwardsiella tarda (어류 병원성 균주 Edwardsiella tarda에 대한 키토산-실버 나노입자의 항박테리아 효과)

  • Dananjaya, S.H.S.;Godahewa, G.I.;Lee, Youngdeuk;Cho, Jongki;Lee, Jehee;De Zoysa, Mahanama
    • Journal of Veterinary Clinics
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    • v.31 no.6
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    • pp.502-506
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    • 2014
  • Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterial properties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were $25{\mu}g/mL$ and $125{\mu}g/mL$, respectively. The field emission scanning electron microscope (FE-SEM) image of CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore, treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentration and time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition, MTT assay results showed that the lowest cell viability at $100{\mu}g/mL$ of CAgNCs treated E. tarda. Overall results of this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.

The Basic Study on the Preventive Measures against Medical Accident Induced by Acupuncture and Moxibustion Therapy I. A Literature Research on the Essence of Prohibitive Acupuncture Point, Jīn-Zhėn-Xué-Gė (禁鍼穴歌) (침구 의료행위에 기인하는 의료사고 예방에 관한 기초적 연구 (I) - 금침혈가에 관한 문헌적 고찰 -)

  • Moon, Jin-Young
    • The Journal of Dong Guk Oriental Medicine
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    • v.8 no.2
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    • pp.175-199
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    • 2000
  • Acupuncture and moxibustion play an important role not only in the medical service and health care of Korea, China and japan but also in those of many western countries such as Germany, France, United States of America. An unexpected results induced by careless acupuncture and moxibustion treatments may be mild or severe, and are due to practitioner's mistake and particular body condition of a patient. This study was performed to establish a preventive measures against medical accident induced by acupuncture therapy. Total 23 acupuncture points which are included in a J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ were selected as prohibited acupuncture points. Although the records in J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ are an important data historically which show dangerous acupuncture points, there are few a full investigation on J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ up to the present. So present study was done for literature review to understand a feasibility of the records in J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ in association with dangerous acupuncture points. Based upon the locations of these acupuncture points, the dangerous region were classified three categories, head, soma and limb. And abnormal signs related to the improperly performed acupuncture treatment on each point were investigated by using historical reference books on acupuncture and moxibustion. And then names, locations, depth of insertion, operation methods and side effects of each point were investigated.

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Microbial Detection and Identification Using Biosensors

  • Kim, Sol
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2008.05a
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    • pp.135-135
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    • 2008
  • Various biosensors were evaluated for identifying and detecting foodborne pathogens in a rapid and effective manner. First, five strains of Escherichia coli and six strains of Salmonella were identified using Fourier transform infrared spectroscopy and a statistical program. For doing this, lipopolysaccharides (LPSs) and outer membrane proteins (OMPs) were extracted from a cell wall of each bacterial strain. As a result, each strain was identifed at the level of 97% for E. coli and 100% for Salmonella. Second, E. coli O157:H7, S. Enteritidis, and Listeria monocytogenes were identified by multiplex PCR products from four specific genes of each bacteria using a capillary electrophoresis (CE). Also, ground beef for E. coli O157:H7, lettuce for S. Enteritidis, and hot dog for L. monocytogenes were used to determine the possibility of detecting pathogens in foods. Foods inoculated with respective pathogen were cultivated for six hours and multiplex PCR products were obtained and assessed. The minimum detection levels of tested bacteria were <10 cells/g, <10 cells/g, and $10^4$ cells/g for E. coli O157:H7, S. Enteritidis, and L. monocytogenes, respectively. Third, it was possible to detect S. Typhimurium in a pure culture and lettuce by a bioluminescence-based detection assay using both recombinant bacteriophage P22::luxI and a bioluminescent bioreporter. In addition, bacteriophage T4 was quantitatively monitored using E. coli including luxCDABE genes.

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Isolation, In vitro Antibacterial Activity, Bacterial Sensitivity and Plasmid Profile of Lactobacilli

  • Lonkar, P.;Harne, S.D.;Kalorey, D.R.;Kurkure, N.V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.9
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    • pp.1336-1342
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    • 2005
  • The present research work was conducted to evaluate the beneficial effects as well as the safety aspects of lactobacilli as probiotic. Lactobacilli were isolated from poultry faecal samples, feed samples and from some known preparations procured from poultry feed manufacturers. L. acidophilus and L. sporogenes were tested for the antibacterial activity against four poultry pathogens viz. Escherichia coli, Salmonella spp., Proteus spp. and Pseudomonas aeruginosa. Cell free supernatant (CFS) of L. acidophilus exhibited significantly higher antibacterial activity against Salmonella spp. at original pH (4.50${\pm}$0.02). At the adjusted pH (6.50${\pm}$0.02) significantly higher antibacterial activity was recorded against indicator organism except for P. aeruginosa. Likewise, L. sporogenes exhibited similar antibacterial activity at original as well as adjusted pH except for E. coli. Antibacterial activity against E. coli was significantly higher at adjusted pH than at original pH of CFS. The competitive exclusion of E. coli by lactobacilli over the intestinal epithelial cells (IEC) was checked. L. acidophilus strain I, which was of poultry origin, exhibited maximum attachment over IEC as compared to other three strains of non-poultry origin viz. L. acidophilus strain II, L. sporogenes strain I and II. Overall, L. acidophilus exhibited higher competitive exclusion as compared to L. sporogenes. All the lactobacilli of poultry origin were most sensitive to penicillin G, amoxycillin, ampicillin and chloramphenicol, least sensitive to sulphamethizole, ciprofloxacin, neomycin, norfloxacin and pefloxacin and resistant to metronidazole and nalidixic acid. The isolates from probiotic preparations were most sensitive to ampicillin, amoxycillin and tetracycline, least sensitive to sulphamethizole, norfloxacin, neomycin and ceftriazone and resistant to nalidixic acid and metronidazole. Eight of the multiple drug resistant lactobacilli isolates were studied for the presence of plasmids. Plasmids could be extracted from six isolates of lactobacilli. These plasmids could be responsible for bacteriocin production or for antibiotic resistance of the strains. The lactobacilli need further studies regarding their safety for use in the probiotic preparations.

THE ASYMPTOTIC BEHAVIOUR OF THE AVERAGING VALUE OF SOME DIRICHLET SERIES USING POISSON DISTRIBUTION

  • Jo, Sihun
    • East Asian mathematical journal
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    • v.35 no.1
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    • pp.67-75
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    • 2019
  • We investigate the averaging value of a random sampling of a Dirichlet series with some condition using Poisson distribution. Our result is the following: Let $L(s)={\sum}^{\infty}_{n=1}{\frac{a_n}{n^s}}$ be a Dirichlet series that converges absolutely for Re(s) > 1. If $X_t$ is an increasing random sampling with Poisson distribution and there exists a number $0<{\alpha}<{\frac{1}{2}}$ such that ${\sum}_{n{\leq}u}a_n{\ll}u^{\alpha}$, then we have $${\mathbb{E}}L(1/2+iX_t)=O(t^{\alpha}{\sqrt{{\log}t}})$$, for all sufficiently large t in ${\mathbb{R}}$. As a result, we get the behaviour of $L({\frac{1}{2}}+it)$ such that L is a Dirichlet L-function or a modular L-function, when t is sampled by the Poisson distribution.

Antibacterial activity of isothiocyanates from cruciferous vegetables against pathogenic bacteria in olive flounder (십자화과 채소 유래 isothiocyanates의 넙치 어병세균에 대한 항균활성)

  • Ko, Mi-Ok;Ko, Jeong-Yeon;Kim, Mi-Bo;Lim, Sang-Bin
    • Food Science and Preservation
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    • v.22 no.6
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    • pp.886-892
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    • 2015
  • The antimicrobial effects of ten isothiocyanates (ITCs) present in cruciferous vegetables and radish root hydrolysate were investigated against pathogenic bacteria from olive flounder. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured against two gram-positive bacterial strains (Streptococcus parauberis, S. iniae) and four gram-negative bacterial strains (Edwardsiella tarda, Vibrio ichthyoenteri, V. harveyi, Photobacterium damselae) by using a broth microdilution technique. The antibacterial activity of ITCs was in the order sulforaphane > sulforaphene > phenylethyl ITC > erucin > benzyl ITC > iberin > I3C > allyl ITC > phenyl ITC > hexyl ITC. The susceptibility of fish pathogens to ITCs was in the order of V. harveyi > E. tarda > P. damselae > S. parauberis > S. iniae > V. ichthyoenteri. Antimicrobial activity (MIC) of radish root hydrolysate was 0.250 mg/mL against S. iniae, 0.438 mg/mL against S. parauberis, and 0.500 mg/mL against both E. tarda and V. harveyi. The aliphatic ITCs were potent inhibitors of the growth of fish pathogens, followed by aromatic ITCs and indolyl ITC. The presence of a double bond in the chemical structure of ITCs decreased antibacterial activity, while ITCs with a thiol (-S-) group and a longer carbon chain increased antibacterial activity. These results suggest that ITCs have strong antibacterial activities and may be useful in the prevention of fish pathogens.