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Chitosan Silver Nano Composites (CAgNCs) as Antibacterial Agent Against Fish Pathogenic Edwardsiella tarda  

Dananjaya, S.H.S. (College of Veterinary Medicine (BK21 Plus Program) and Research Institute of Veterinary Medicine, Chungnam National University)
Godahewa, G.I. (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Lee, Youngdeuk (Korea Institute of Ocean Science Technology)
Cho, Jongki (College of Veterinary Medicine (BK21 Plus Program) and Research Institute of Veterinary Medicine, Chungnam National University)
Lee, Jehee (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
De Zoysa, Mahanama (College of Veterinary Medicine (BK21 Plus Program) and Research Institute of Veterinary Medicine, Chungnam National University)
Publication Information
Journal of Veterinary Clinics / v.31, no.6, 2014 , pp. 502-506 More about this Journal
Abstract
Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterial properties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were $25{\mu}g/mL$ and $125{\mu}g/mL$, respectively. The field emission scanning electron microscope (FE-SEM) image of CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore, treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentration and time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition, MTT assay results showed that the lowest cell viability at $100{\mu}g/mL$ of CAgNCs treated E. tarda. Overall results of this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.
Keywords
antimicrobial agents; Chitosan-silver nanocomposites; Edwardsiellosis; Edwardsiella tarda; reactive oxygen species;
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