• Asian-Australasian Journal of Animal Sciences
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• 제32권12호
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• pp.1864-1872
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• 2019

Objective: This study was conducted to evaluate the effects of Ecklonia stolonifera (E. stolonifera) extract addition on in vitro ruminal fermentation characteristics, methanogenesis and microbial populations. Methods: One cannulated Holstein cow ($450{\pm}30kg$) consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (09:00 and 17:00) at 2% of body weight with free access to water and mineral block were used as rumen fluid donors. In vitro fermentation experiment, with timothy hay as substrate, was conducted for up to 72 h, with E. stolonifera extract added to achieve final concentration 1%, 3%, and 5% on timothy hay basis. Results: Administration of E. stolonifera extract to a ruminant fluid-artificial saliva mixture in vitro increased the total gas production. Unexpectedly, E. stolonifera extracts appeared to increase both methane emissions and hydrogen production, which is contrasts with previous observations with brown algae extracts used under in vitro fermentation conditions. Interestingly, real-time polymerase chain reaction indicated that as compared with the untreated control the ciliate-associated methanogen and Fibrobacter succinogenes populations decreased, whereas the Ruminococcus flavefaciens population increased as a result of E. stolonifera extract supplementation. Conclusion: E. stolonifera showed no detrimental effect on rumen fermentation characteristics and microbial population. Through these results E. stolonifera has potential as a viable feed supplement to ruminants.

• 한국수산과학회지
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• 제42권4호
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• pp.360-365
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• 2009

The dietary fiber and crude fiber contents of different thallus regions (blade, stipe, and holdfast) in three brown algae (Laminaria japonica, Ecklonia stolonifera, and E. cava) were detennined at different ages, and then compared with one another. On a dry matter basis, the soluble dietary fiber (SDF) content was highest ($10.8{\pm}0.5%$) in the holdfast of 2-year old L. japonica, and the insoluble dietary fiber (IDF), total dietary fiber (TDF), and crude fiber (CF) contents were highest in the holdfast of 2-year old E. cava at $44.5{\pm}0.7%$, $50.2{\pm}0.5%$, $6.8{\pm}0.7%$, respectively. The IDF, TDF, and CF contents of these three species were measured in the following order: holdfast > stipe > blade, and the SDF contents of L. japonica exhibited the reverse of this trend. The TDF/CF ratio of 1-year old L. japonica, E. stolonifera, and E. cava was greater than was observed in the corresponding 2-year old samples. This is, to the best of our knowledge, the first report demonstrating that the holdfasts of L. japonica, E. stolonifera, and E. cava are rich in dietary fiber contents, especially IDF, TDF, and CF.

• Fisheries and Aquatic Sciences
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• 제11권1호
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• pp.1-6
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• 2008

In an effort to discover an alternative therapeutic agent against methicillin-resistant Staphylococcus aureus (MRSA), several medicinal plants and seaweeds were evaluated for its antibacterial activity against MRSA. A methanolic extract of the Phaeophyta Ecklonia stolonifera exhibited significant antibacterial activity against MRSA. To perform more detailed investigation on antibacterial activity, the methanol extract of E. stolonifera was further fractionated with organic solvents such as hexane, dimethylchloride, ethyl acetate, and n-butanol. Among them, the hexane fraction showed the strongest antibacterial activity against MRSA strains with MIC from 500 to $600 {\mu}g/mL$. The fraction also exhibited a bactericidal activity against MRSA, indicating that E. stolonifera contains a bactericidal substance against MRSA.

• 생명과학회지
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• 제25권7호
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• pp.780-785
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• 2015

전복을 4일간 굶긴 후 phlorotannin-고함유 곰피를 제공하여 이의 섭취 및 phlorotannin 축적정도를 조사하였다. 전복의 성장속도는 곰피 혹은 일반 사료로 쓰이는 다시마를 20일간 투여한 경우 서로 비슷하였다. 주된 phlorotannin 성분인 7-phloroeckol 및 eckol은 역상 HPLC로서 측정하였다. 7-phloroeckolol은 섭취 6일만에 전복 건조육 1g당 0.58±0.13 mg 정도 축적되었다. Eckol은 6일만에 전복 건조육 1g당 0.25±0.05 mg 정도 축적되었으며 실험기간 내 그 정도를 유지하였다. 대조군로서의 다시마 투여 전복은 phlorotannin을 전혀 함유하지 않았다. Phlorotannin은 전복 조직내에서의 구성효소들에 의하여 분해되어 지는것으로 보인다. 그러므로 다양한 생리활성을 지닌 phlorotannin 물질들을 가진 고부가 가치의 전복 생산은 전복수확 6일전부터 간단히 사료만 phlorotannin-고함유 곰피로 바꾸어 공급함으로서 생산가능하다.

• 식품과학과 산업
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• 제51권4호
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• pp.334-342
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• 2018

People in Korea, a peninsular, have acquired a variety of marine food resources including seaweeds. Ecklonia stolonifera, a brown algae, is commonly dwelling in Korean coasts and their cultivation methods were developed for a mass-production. Recently, studies have revealed that Ecklonia stolonifera is a promising material for the development of health functional foods. In an effort to carefully review the current understating in the effects and mechanisms of Ecklonia stolonifera on liver functions by deduction from relevant literatures, the effective components were identified as phlorotannins, including dieckol, eckstolonol, eckol, phlorofucofuroeckol A, and phlorosterol. Their aiding action on the hepatic functions is categorized as follows. A) Regulation of oxidative stress by anti-oxidant capacity, B) Protection of hepatocytes from toxins, C) Prevention of alcoholic fatty liver and fibrogenesis, D) Regulation of chronic disease by improvement of inflammatory responses and lipid metabolisms, and E) indirect benefit conferred by a personal total wellness.

• 한국식품영양과학회지
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• 제25권6호
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• pp.1069-1073
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• 1996

해조류의 기능특성 해석의 일환으로 해조류 중 갈조류인 미역 (Undaria pinnattnda), 감태 (Ecklonia stolonifera), 곰피(Ecklonia cava), 다시마(Laminaria japonica) 및 모자반(Sargussum fulvellum)과 녹조류인 청각(Codium fragile), 파래(Enteromorpha compressa) 및 홍조류인 김(Porphyra tenera)등을 시료로 하여, 생체내 퓨린대사에서 xanthine 및 hypoxanthine으로 부터 요산을 형성, 혈액 중 이상 고요산 증상에 의하여 신장질환 및 통풍을 유발하는 효소인 xanthine oxidase 저해작용에 대하여 살펴 본 결과, 갈조류의 다시마과 Ecklonia 속인 감태와 곰피가 가장 높은 저해율을 나타내었으며, 용매 추출물에 있어서는 diethyl ether>ethyl acetate>chloroform>수용성 획분의 순으로 나타났다. 또한 xanthine oxidase 저해작용이 가장 높은 감태 diethyl ether 추출물을 silica gel column chromatography, thin layer chromatography 및 HPLC를 통하여 xanthine of idase 저해 인자를 분리한 결과, xanthine oxidase 저해작용이 가장 강한 활성성분은 420, 456, 467nm에서 흡수파장을 가지는 물질로서, 이는 siphonaxanthine인 것으로 추정되었다.

• Archives of Pharmacal Research
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• 제27권2호
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• pp.194-198
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• 2004

Reactive oxygen species (ROS) play an important role in the pathogenesis of many human degenerative diseases such as cancer, aging, arteriosclerosis, and rheumatism. Much attention has been focused on the development of safe and effective antioxidants. To discover sources of antioxidative activity in marine algae, extracts from 17 kinds of seaweed were screened for their inhibitory effect on total ROS generation in kidney homogenate using 2',7'-dichlorofluorescein diacetate (DCFH-DA). ROS inhibition was seen in three species: UIva pertusa, Symphyocladia latiuscula, and Ecklonia stolonifera. At a final concentration of 25 $\mu\textrm{g}$/mL, U. pertusa inhibited 85.65$\pm$20.28％ of total ROS generation, S. latiscula caused 50.63$\pm$0.09％ inhibitory, and the Ecklonia species was 44.30$\pm$7.33％ inhibition. E. stolonifera OKAMURA (Lam-inariaceae), which belongs to the brown algae, has been further investigated because it is commonly used as a foodstuff in Korea. Five compounds, phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5), isolated from the ethyl acetate soluble fraction of the methanolic extrclct of E. stolonifera inhibited total ROS generation.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.265.3-266
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• 2003

Ecklonia stolonifera Okamura is a member of the family Laminariaceae, belonging to the order Laminariales. Previously we reported that the methanolic extract of the brown alga E stolonifera exerts antioxidative activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. In the course of a continuous study on the active principles of this alga, a new phlorotannin, named eckstolonol (2), was isolated along with the four known phlorotannins i.e. (omitted)

• 한국수산과학회지
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• 제43권3호
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• pp.231-238
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• 2010

The Induction of regeneration and maturation in the free-living gametophytes of Ecklonia stolonifera Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol\;m^{-2}s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, 10 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $20^{\circ}C$, 40 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D). The sexual maturation of female and male gametophytes was facilitated at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}s^{-1}$, 14:10 h (L:D). These results provide basic information for controlling the regeneration and maturation of the free-living gametophytes for artificial seed production of E. stolonifera.

• 한국환경과학회지
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• 제18권10호
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• pp.1065-1070
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• 2009

Experimental design for ecological restoration approach was investigated from the P' company's slag dumping area with higher pH value. The degree of pH recovery was measured by the injection of carbon dioxide from the LNG exhaust gas, and the residual carbon, for example, calcium and carbonate which can be controlled by artificial seaweed beds. The degree of adaptability from 3 algaes (Undaria pinnatifida, Sargassum horneri and Ecklonia stolonifera) and uptake nutrient function of Ecklonia stolonifera chosen in the first treatment were measured in the laboratory to determine the transplanting algae in artificial seaweed beds. The higher value of pH was decreased to 7.0~8.5 by injection of LNG exhaust gas with flow rate $20\;m^3/min$. In the experiment design at laboratory, the upper part of frond of Undaria pinnatifida and Sargassum horneri began to decay, and the color changed after 10 days. However, those of Ecklonia stolonifera were after 14~20 days. The uptake rate of nutrient from Ecklonia stolonifera was higher than those of other algaes, and those was similar pattern in the control (e.g. seawater); The DIN concentration uptake of Ecklonia stolonifera was $1.88\;{\mu}g/L/hr$ avg. in leachate, but $2.19\;{\mu}g/L/hr$ avg. in seawater. However, the ${PO_4}^3$-P concentration uptake was $0.18\;{\mu}g/L/hr$ avg. in leachate, but $0.31\;{\mu}g/L/hr$ avg. in seawater. These result indicated Ecklonia stolonifera uptaked these nutrients in the leachate as well as in seawater, and it may suggested for this species to transplant for constructing artificial seaweed beds.