• Title/Summary/Keyword: E. coli(K99)

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Prevalence of pili and enterotoxins of Escherichia coli associated with diarrhea in preweaning piglets (포유자돈 소장에서 분리된 대장균의 섬모항원과 장내독소 분포양상)

  • Ham, Hee-jin;Cheon, Doo-sung;Chae, Chan-hee
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.779-784
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    • 1997
  • A comprehensive study of 132 Escherichia coli isolates from 150 piglets with colibacillosis included detection of heat-labile enterotoxin, heat-stable enterotoxin, and identification of K88 (F4), K99 (F5), 987P (F6), and F41. Four pili were examined by haemagglutination and slide agglutination test. Heat-labile(LT) and heat-stable(ST) enterotoxin was determined by reverse passive latex agglutination and precipitation test, respectively. Among 132 E coli isolates, 26 had K88 (19.7%), 16 had K99 (12.1%), 3 had 987P (2.3%), and 2 had F41 (1.5%). Three had K88 and K99 (2.3%), 3 had K88 and 987P (2.3%), 2 had K99 and 987P (1.5%), 5 had K99 and F41 (3.8%), and 8 E coli strains had K88, K99 and F41 (6.1%) simultaneously. Among 132 E coli isolates, 5 produced LT only (3.8%), 55 produced heat-stable toxin ST only (41.7%), and 4 produced both LT and ST (3.0%). Three major pathotypes accounted for 27.9% of E coli isolates: $K99^+$ (8.3%), $K88^+ST^+$ (9%) and $K88^+$ (10.6%). Results of this study indicated that piliated enterotoxin-producing E coli was prevalent and was associated with diarrhea in preweaning piglets.

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Detection of Escherichia coli(K99), Clostridium perfringens and Cryptosporidium parvum in Diarrhetic Feces of Korean Native Calves (한우 송아지의 설사 분변에서 Escherichia coli(K99), Clostridium perfringens와 Cryptosporidium parvum의 검출)

  • Chon, Seung-Ki;Lee, Han-Kyung;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.24 no.4
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    • pp.588-592
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    • 2007
  • The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.

Enterotoxin Production and Plasmid Detection of Citrate Utilizing Escherichia coli Isolated from Cattle (우(牛) 유래(由來) Citrate이용(利用) 대장균(大腸菌)의 장독소(腸毒素) 산생능(産生能) 및 Plasmid DNA)

  • Chae, Tae-chul;Choi, Won-pil
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.59-65
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    • 1988
  • This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli ($Cit^+$ E. coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 $Cit^+$ E. coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains(11) were untypable. Thirty-nine(81.3%) out of 48 $Cit^+$ E. coli were produced colicin and 13(27.0%) were produced hemolysin. Of 12 $Cit^+$ E. coli bearing K99 antigen, 6(50.0%) were produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 $Cit^+$ E. coli. It's molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid was commonly existed in all strains.

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Enhanced Heterologous Expression of Aspergillus niger Epoxide Hydrolase and Its Application to Enantioselective Hydrolysis of Racemic Epoxides (Aspergillus niger의 Epoxide Hydrolase 고효율 발현 및 라세믹 에폭사이드의 입체선택적 가수분해)

  • Lee, Soo Jung;Kim, Hee Sook;Lee, Eun Yeol
    • Applied Chemistry for Engineering
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    • v.17 no.5
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    • pp.557-560
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    • 2006
  • The epoxide hydrolase (EH) of Aspergillus niger LK was expressed to high levels in Escherichia coli based on codon usage. E. coli, Rosetta (DE3)PLysS, containing a large number of tRNAs for rare-codons was employed as a host strain. The recombinant E. coli expressing A. niger EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee was obtained from racemic substrates.

Kinetic analysis of E. coli disinfection using UV-LED (LED 광원 UV에 의한 대장균(E. coli) 소독의 속도론 해석)

  • Kim, Kyeong-Rae;Chang, In-Soung
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.6
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    • pp.489-496
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    • 2021
  • Water disinfection using UV-LED(Light emitting diode) has many advantages, such as smaller footprint and power consumption as well as relatively longer lifespan than those of conventional mercury-UV lamps. Moreover, UV-LED disinfection is considered an environmentally benign process due to its mercury-free nature. In this study, disinfection using an LED module emitting 275nm UV was carried out. 384 UV-LEDs were put into a cylinder tube with a capacity of 1.7 liters. The UV intensity of the UV-LED module was controlled from 1.7 to 8.4 mW/cm2. The disinfection efficiency for the model microorganism solutions(E. coli ) was monitored. As the UV intensity(I) and contact time(t) varied, inactivation of the microorganisms from 2 to 4-log-removals(i.e., 99 to 99.99% of disinfection efficiency) was achieved. Disinfection using UV-LED was followed to 1st order reaction and the reaction rate constant, k was determined. In addition, the relationship between UV intensity(I) and contact time(t) in order to obtain 99.99% of disinfection efficiency was modeled: I1.2·t= 460, which indicates that the product of UV intensity and contact time requiring 4-log-removals is always constant.

Characterization of cefotaxime-resistant Escherichia coli isolated from wastewater treatment plant in Daegu (대구지역 폐수처리장에서 분리한 cefotaxime-resistant Escherichia coli의 특성)

  • Kim, Hwan-Deuk;Park, Dae-Hyun;Lee, Mi-Ree;Kim, Eun-Jeong;Cho, Jae-Keun
    • Korean Journal of Veterinary Service
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    • v.37 no.4
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    • pp.225-231
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    • 2014
  • In this study, 185 cefotaxime-resistant Escherichia coli were isolated from different stages of a wastewater treatment plant (WWTP) in Daegu in Korea. Among them, 99.5% (184 isolates) originated from raw sewage and 0.5% (1 isolates) from the final effluent. Cefotaxime-resistant E. coli were high resistant to ampicillin, piperacillin, cefazolin, cephalothin, cefachlor and cefamandole (99.5~100%). About 93% of the cefotaxime-resistant E. coli were extended-spectrum ${\beta}$-lactamases (ESBL)-producing E. coli. The $bla_{TEM+CTX}$ gene was the most predominant of the ESBL genes (72.5%), followed by $bla_{CTX-M}$ (16.2%), $bla_{TEM}$ (8.7%), $bla_{TEM+CTX+SHV}$ (1.1%), $bla_{TEM+SHV}$, $bla_{TEM+OXA}$, and $bla_{TEM+CTX+SHV}$ (respectvely 0.5%). Class 1 and 2 integron were found in 49.7% and class 3 integron was not found. All of integron positive isolates were multiresistant (i.e. resistant to four or more antibiotics). Our findings showed WWTP is contaminated with antibiotic resistant bacteria with resistance genes.

Sterilization of Scoria Powder by Corona Discharge Plasma (코로나 방전 플라즈마를 이용한 화산암재 분말 살균)

  • Jo, Jin Oh;Lee, Ho Won;Mok, Young Sun
    • Applied Chemistry for Engineering
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    • v.25 no.4
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    • pp.386-391
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    • 2014
  • Atmospheric-pressure nonthermal corona discharge plasma was applied to the sterilization of biologically contaminated scoria powder. Escherichia coli (E. coli) culture solution was uniformly sprayed throughout the scoria powder for artificial inoculation, which was well mixed to ensure uniformity of the batch. The effect of the key parameters such as discharge power, treatment time, type of gas and electrode distance on the sterilization efficiency was examined and discussed. The experimental results revealed that the plasma treatment was very effective for the sterilization of scoria powder; 5-min treatment at 15 W could sterilize more than 99.9% of E. coli inoculated into the scoria powder. Increasing the discharge power, treatment time or applied voltage led to an improvement in the sterilization efficiency. The effect of type of gas on the sterilization efficiency was in order of oxygen, synthetic air (20% oxygen) and nitrogen from high to low. The inactivation of E. coli under the influence of corona discharge plasma can be explained by cell membrane erosion or etching resulting from UV and reactive oxidizing species (oxygen radical, OH radical, ozone, etc.), and the destruction of E. coli cell membrane by the physical action of numerous corona streamers.

Development of Recombinant Escherichia coli Expressing Rhodotorula glutinis Epoxide Hydrolase (Rhodotorula glutinis의 epoxide hydrolase 고효율 발현 유전자 재조합 Escherichia coli 생촉매 개발)

  • Lee Soo-Jung;Kim Hee-Sook
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.415-419
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    • 2006
  • The epoxide hydrolase (EH) of Rhodotorula glutinis which has a high enantioselectivity against aromatic epoxide substrates was expressed to high levels in Escherichia coli based on codon usage. We analysed the Preference of codon usage between the yeast, R. glutinis, and bacteria, E. coli. E. coli, Rosetta(DE3)pLysS, harbors pRARE plasmid with tRNA genes for rare-codons was employed as a host strain. The recombinant E. coli expressing R. glutinis EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee (enantiomeric excess) was obtained from racemic substrates.

Antibacterial Activities of Mushroom Liquid Culture Extracts Against Livestock Disease-Causing Bacteria and Antibiotic Resistant Bacteria (가축질병 세균 및 항생제 내성 세균에 대한 버섯 균사체 배양 추출물의 항균 활성)

  • Park, Joo-Woong;Kim, Taeg;Lim, Dong-Jung;Lee, Hyang-Burm;Joo, Yi-Seok;Park, Yong-Il
    • The Korean Journal of Mycology
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    • v.32 no.2
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    • pp.145-147
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    • 2004
  • The ethyl acetate extracts from the liquid cultures of Coriolus versicolor, Phellinus linteus, and Hericium erinaceus showed significant antibacterial activities against Escherichia coli K88, E. coli K99, E. coli 987P, and Salmonella typhimurium 14058 causing bacterial diarrhea in Korean house pigs and chicken. Of these extracts, Coriolus versicolor extract showed the highest antibacterial activity. In addition, these extracts also showed significant growth inhibition against Staphylococcus aureus CARM3230 and E. coli CARM1381 which are known as kanamycin and ampicillin-resistant strains. These results showed that the mushroom extracts could be developed as a livestock feed additives that can replace commercial antibiotics, and also could be good resources for the development of a new antibacterial agent.

Evaluation of Optimal Condition for Recombinant Bacterial Ghost Vaccine Production with Four Different Antigens of Streptococcus iniae-enolase, GAPDH, sagA, piaA (연쇄구균증 항원-enolase, GAPDH, sagA, piaA에 대한 재조합 고스트 박테리아 백신의 생산 최적화)

  • Ra, Chae-Hun;Kim, Yeong-Jin;Son, Chang-Woo;Jung, Dae-Young;Kim, Sung-Koo
    • Journal of Life Science
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    • v.19 no.7
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    • pp.845-851
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    • 2009
  • A vector harboring double cassettes; a heterologous gene expression cassette of pHCE-InaN-antigen and a ghost formation cassette of pAPR-cI-E lysis 37 SDM was constructed and introduced to E. coli DH5a. For the production of a bacterial ghost vaccine, bacterial ghosts from E. coli / Streptococcus iniae with four different types of antigens - enolase, GAPDH, sagA and piaA - were produced by the optimization of fermentation parameters such as a glucose concentration of 1 g/l, agitation of 300 rpm and aeration of 1 vvm. Efficiency of ghost bacteria formation was evaluated with cultures of OD$_{600}$=1.0, 2.0 and 3.0. The efficiency of the ghost bacteria formation was 99.54, 99.67, 99.99 and 99.99% with inductions at OD$_{600}$=3.0, 1.0, 2.0 and 1.0 for E. coli/S. iniae antigens enolase, piaA, GAPDH and sagA, respectively. Ghost bacteria as a vaccine was harvested by centrifugation. The antigen protein expressions were analyzed by SDS-PAGE and western blot analysis, and the molecular weights of the enolase, piaA, GAPDH and sagA were 78, 26, 67 and 26 kDa, respectively. The molecular weights of the expressed antigens were consistent with theoretical sizes obtained from the amino acid sequences.