Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Characterization of cefotaxime-resistant Escherichia coli isolated from wastewater treatment plant in Daegu

대구지역 폐수처리장에서 분리한 cefotaxime-resistant Escherichia coli의 특성

  • Kim, Hwan-Deuk (Metropolitan Health & Enviornmental Research Institute) ;
  • Park, Dae-Hyun (Metropolitan Health & Enviornmental Research Institute) ;
  • Lee, Mi-Ree (Metropolitan Health & Enviornmental Research Institute) ;
  • Kim, Eun-Jeong (Metropolitan Health & Enviornmental Research Institute) ;
  • Cho, Jae-Keun (Metropolitan Health & Enviornmental Research Institute)
  • 김환득 (대구광역시보건환경연구원) ;
  • 박대현 (대구광역시보건환경연구원) ;
  • 이미리 (대구광역시보건환경연구원) ;
  • 김은정 (대구광역시보건환경연구원) ;
  • 조재근 (대구광역시보건환경연구원)
  • Received : 2014.08.19
  • Accepted : 2014.11.21
  • Published : 2014.12.30
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Abstract

In this study, 185 cefotaxime-resistant Escherichia coli were isolated from different stages of a wastewater treatment plant (WWTP) in Daegu in Korea. Among them, 99.5% (184 isolates) originated from raw sewage and 0.5% (1 isolates) from the final effluent. Cefotaxime-resistant E. coli were high resistant to ampicillin, piperacillin, cefazolin, cephalothin, cefachlor and cefamandole (99.5~100%). About 93% of the cefotaxime-resistant E. coli were extended-spectrum ${\beta}$-lactamases (ESBL)-producing E. coli. The $bla_{TEM+CTX}$ gene was the most predominant of the ESBL genes (72.5%), followed by $bla_{CTX-M}$ (16.2%), $bla_{TEM}$ (8.7%), $bla_{TEM+CTX+SHV}$ (1.1%), $bla_{TEM+SHV}$, $bla_{TEM+OXA}$, and $bla_{TEM+CTX+SHV}$ (respectvely 0.5%). Class 1 and 2 integron were found in 49.7% and class 3 integron was not found. All of integron positive isolates were multiresistant (i.e. resistant to four or more antibiotics). Our findings showed WWTP is contaminated with antibiotic resistant bacteria with resistance genes.

Keywords

References

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