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Enhanced Heterologous Expression of Aspergillus niger Epoxide Hydrolase and Its Application to Enantioselective Hydrolysis of Racemic Epoxides  

Lee, Soo Jung (Department of Food Science and Technology, College of Engineering, Kyungsung University)
Kim, Hee Sook (Department of Food Science and Technology, College of Engineering, Kyungsung University)
Lee, Eun Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University)
Publication Information
Applied Chemistry for Engineering / v.17, no.5, 2006 , pp. 557-560 More about this Journal
Abstract
The epoxide hydrolase (EH) of Aspergillus niger LK was expressed to high levels in Escherichia coli based on codon usage. E. coli, Rosetta (DE3)PLysS, containing a large number of tRNAs for rare-codons was employed as a host strain. The recombinant E. coli expressing A. niger EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee was obtained from racemic substrates.
Keywords
epoxide hydrolase; Aspergillus niger; enantioselective hydrolysis; Escherichia coli Rosetta; codon usage;
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1 E. Y. Lee, Kor. J. Biotechnol. Bioeng., 17, 321 (2002)
2 M. A. Argiriadi, C. Morisseau, M. H. Goodrow, D. L. Dowdy, B. D. Hammock and D. W. Christianson, J. Biol. Chem., 275, 15265 (2000)   DOI   ScienceOn
3 T. Yamada, C. Morisseau, J. E. Maxwell, M. A. Argiriadi, D. W. Christianson, and B. D. Hammock, J. Biol. Chem., 275, 23082 (2000)   DOI   ScienceOn
4 Y. Nakamura, T. Gojobori, and T. Ikemura, Nucleic Acids Res., 28, 292 (2000)   DOI   ScienceOn
5 M. Arand, F. Muller, A. Mecky, W. Hinz, P. Urban, D. Pompon, R. Kellner, and F. Oesch, Biochem. J., 337, 37 (1999)   DOI   ScienceOn
6 K. M. Manoj, A. Archelas, J. Baratti, and R. Furstoss, Tetrahedron, 57, 695 (2001)   DOI   ScienceOn
7 R. Novy, D. Drott, K. Yaeger, and R. Mierendorf, Newsletter of Novagen., 12, 1 (2001)
8 E. Y. Lee and H. S. Kim, Kor. J. Biotechnol. Bioeng., 16, 562 (2001)
9 P. Besse and H. Veschambre, Tetrahedron 50, 8885 (1994)   DOI   ScienceOn
10 W. J. Choi, E. C. Huh, H. J. Park, E. Y. Lee, and C. Y. Choi, Biotechnol. Techniques, 12, 225 (1998)   DOI
11 H. Visser, S. Vreugdenhil, J. A. M. de Bont, and J. C. Verdoes, Appl. Microbiol. Biotechnol., 53, 415 (2000)   DOI   ScienceOn
12 M. Nardini, I. S. Ridder, H. J. Rozeboom, K. H. Kalk, R. Rink, D. B. Janssen, and B. W. Dijkstra, J. Biol. Chem., 274, 14579 (1999)   DOI   ScienceOn
13 A. Steinreiber and K. Faber, Current Opinion in Biotechnol., 12, 552 (2001)   DOI   ScienceOn
14 J. Zou, B. M. Hallberg, T. Bergfors, F. Oesch, M. Arand, S. L. Mowbray, and T. A. Jones, Structure, 8, 111 (2000)   DOI   ScienceOn
15 C. A. G. M. Weijers and J. A. M. de Bont, J. Mol. Catal. B: Enzym., 6, 199 (1999)   DOI   ScienceOn
16 A. Archelas and R. Furstoss, Current Opinion in Chem. Biology, 5, 112 (2001)   DOI   ScienceOn
17 M. Arand, H. Hemmer, H. Durk, J. Baratti, A. Archelas, and R. Furstoss, Biochem. J., 344, 273 (1999)   DOI   ScienceOn
18 H. Visser, J. A. M. de Bont, and J. C. Verdoes, Appl. Environ. Microbiol. 65, 5459 (1999)
19 R. Rink, J. H. L. Spelberg, R. J. Pieters, J. Kingma, M. Nardini, R. M. Kellogg, B. W. Dijkstra and D. B. Janssen, J. Am. Chem. Soc., 121, 7417 (1999)   DOI   ScienceOn