• Journal of Plant Biotechnology
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• v.8 no.1
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• pp.9-14
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• 2006

As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

• The KIPS Transactions:PartD
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• v.9D no.4
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• pp.667-676
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• 2002

Digital environment that is represented to Internet is displacing business way of industry and business achievement way with the fast speed being giving great change on life whole. Improve existent business process utilizing Internet and Web connection technology, information superhighway to tradition industrialist manufacturer and e-Transformation's propulsion that wish to maximize productivity and administration efficiency is spread vigorously. In this paper, administration efficiency raising of Web-based factory monitoring system to do monitoring when everywhere integrating operation present condition current point of time of discrete factories in inside and outside of the country by purpose design and implemented. That is, do to normalize system structure and achievement function, and administration data of Web-based, and design using VML (Unified Modeling Language) and take advantage of ASP (Active Server Pages) and implemented web function. Implemented Web-based factory monitoring system is applying two factory (Kl, K2) of K CO., LTD. tire operation division to caravan, and application result was evaluated by very efficient thing to grasp operation situation of whole factory synthetically.

• Korean Journal of Ichthyology
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• v.19 no.2
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• pp.83-87
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• 2007

Edwardsiella tarda, a gram (-) pathogen causing edwardsiellosis in farmed fish, was transformed via electroporation with a plasmid expression vector driving the PhiX174 E-lysis gene under the transcriptional control by lambda PR regulatory sequence. The persistent maintenance of the plasmid vector in recombinant E. tarda was found in numerous subculture procedures over up to 6 months without any adverse effect on the original copy number of plasmids. Comparative examination based on semi-quantitative RT-PCR analysis on transcriptional efficiency of E-lysis gene between recombinant E. coli and E. tarda indicated that promoter strength and induction capacity of bacterial ghosts would be retarded in E. tarda as compared to the E. coli. However, the completeness of induction for bacterial ghosts in E. tarda was the same with E. coli, in which at least 99.99% of induction rate was possible and further the viability of recombinant bacteria was completely eliminated by a post-induction procedure including washing and freeze drying lyophilization.

• Korean Journal of Mathematics
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• v.14 no.2
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• pp.227-232
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• 2006

We study some dynamical properties in the context of bitransformation groups, and show that if (H,X,T) is a bitransformation group such that (H,X) is almost periodic and (X/H,T) is pointwise almost periodic $T_2$ and $x{\in}X$, then $E_x=\{q{\in}E(H,X){\mid}qx{\in}{\overline{xT}\}$ is a compact $T_2$ topological group and $E_{qx}=E_x(q{\in}E(H,X))$ when H is abelian, where E(H,X) is the enveloping semigroup of the transformation group (H,X).

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.27-30
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• 1989

F. velutipes Leu 2 gene (${\beta}-isopropylmalate$ dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.

• Journal of Institute of Control, Robotics and Systems
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• v.16 no.6
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• pp.559-565
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• 2010

In this paper, we present a transformation method of source code from a POSIX (Portable Operating System Interface) based source code into an OSEK/VDX (Offene Systeme und deren Schnittstellen fur die Elektronik in Kraftfahrzeugen/Vehicle Distributed eXecutive) source code. As the electronic parts of automobile systems increase, the use of embedded software in automobile systems is also growing. Accordingly, many electronic systems are designed in automobile system with OSEK/VDX. Otherwise, one of the major problems of embedded software would be portability to other OS's. To enhance the portability and interoperability of embedded software, we propose a source code transformation method from POSIX to OSEK/VDX based on API (Application Programming Interface) translation method. Considering the characteristics of the OSEK/VDX which uses OIL (OSEK/VDX Implementation Language) standard, transformation process is performed with source code transformation and OIL code generation. For the validity of the proposed method, the transformation experiment is given using Micro-C OS II and OSEK/VDX with XC167CI micro-controller.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.12
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• pp.1145-1151
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• 2013

Kinematic modeling is a prerequisite for motion planning and the control of mobile robots. In this paper, we proposed a new method of kinematic modeling for a type of mobile robot based on differential motion transformation. The differential motion implies a small translation and rotation in three-dimensional space in a small time interval. Thus, transformation of the differential motion gives the velocity relationship, i.e., Jacobian between two coordinate frames. Since the theory of the differential motion transformation is well-developed, it is useful for the systematic velocity kinematic modeling of mobile robots. In order to show the validity for application of the differential motion transformation, we obtained velocity kinematic models for a type of exemplar mobile robot including spherical ballbots.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.6
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• pp.1296-1302
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• 1988

The study has been carried out under various experimental conditions to investigate mechanical properties by the transformation conditions of austempered low-alloy ductile cast iron. The amount of retained austenite and bainite after quenching was determined by the X-ray diffractometer and the point counting method and which the microstructure was investigated by the S.E.M. The mechanical properties of austempered low-alloy ductile cast iron can be varried over a comparatively wide range by changing the transformation conditions. During isothermal transformation of austenite in the bainite region, low-alloy ductile cast iron austempered at holding time of 40 minute has the maximum volume fraction(24%) of retained austenite in the cast iron matrix and which optimum values of mechanical properties correspond to the maximum amount of retained austenite, which falls with decreasing transformation temperature. The low values of both tensile strength and elongation in the initial stage of bainite transformation can be explained by premature fracture of tensile specimens and the tensile strength, hardness and elongation do not change considerably after a certain period. With a decreasing transformation temperature the tensile strength increase while the elongation decrease, especially the elongation has the maximum value at temperature $370^{\circ}C$.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.9
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• pp.1680-1685
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• 2010

In this note, a proof of Utkin's theorem is presented for a MI(Multi Input) uncertain linear case. The invariance theorem with respect to the two transformation methods so called the two diagonalization methods are proved clearly and comparatively for MI uncertain linear systems. With respect to the sliding surface transformation and the control input transformation, the equation of the sliding mode i.e., the sliding surface is invariant. Both control inputs have the same gains. By means of the two transformation methods the same results can be obtained. Through an illustrative example and simulation study, the usefulness of the main results is verified.

• Journal of applied mathematics & informatics
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• v.33 no.1_2
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• pp.229-234
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• 2015

In the setting of semidefinite linear complementarity problems on $S^n$, we focus on the Stein Transformation $S_A(X):=X-AXA^T$ for $A{\in}R^{n{\times}n}$ that is positive semidefinite preserving (i.e., $S_A(S^n_+){\subseteq}S^n_+$) and show that such transformation is strictly monotone if and only if it is nondegenerate. We also show that a positive semidefinite preserving $S_A$ has the Ultra-GUS property if and only if $1{\not\in}{\sigma}(A){\sigma}(A)$.