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Reduction of Allergenicity of Wheat Flour by Enzyme Hydrolysis (효소 분해에 의한 밀가루의 항원성 저감화)

  • Park, Ju-Yeon;Ahn, Jeung-Yeub;Hong, Hee-Ok;Hahn, Young-Sook
    • Korean Journal of Food Science and Technology
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    • v.36 no.1
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    • pp.152-157
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    • 2004
  • Gluten was extracted from domestic wheat flour using UTH buffer (4 M urea in 0.1 M Tris-HCl, pH 8.6) and validated by SDS-PAGE analysis for production of wheat flour products with reduced gluten content.. Anti-gluten polyclonal antibody was made by administering extracted gluten fraction on animal model. Anti-gluten serum titer of extracted gluten fraction was evaluated by ELISA, and that of antibody titer according to administration period. Anti-gluten sera were used for ELISA and immunoblot analysis before and after hydrolysis of gluten fraction at optimal pH and temperature condition for each protease. Gluten fraction separated by SDS-PAGE showed several bands covering 75 to 10 kDa, in which anti-gluten sera were 25, 34, and 45 kDa. Enzyme hydrolysis of gluten fraction revealed protein band sizes to be lower than 15 kDa. Content of pretense from bovine pancreas (b.p. protease) for gluten hydrolysis was estimated as 1 mg in 10 mL gluten fraction extracted for 4 hr.

Degradation and Stability of Organic-Inorganic Perovskite Solar Cells (유 무기 페로브스카이트 태양전지의 열화와 안정성)

  • Cho, Kyungjin;Kim, Seongtak;Bae, Soohyun;Chung, Taewon;Lee, Sang-won;Lee, Kyung Dong;Lee, Seunghun;Kwon, Guhan;Ahn, Seh-Won;Lee, Heon-Min;Ko, Min Jae;Kang, Yoonmook;Lee, Hae-seok;Kim, Donghwan
    • Current Photovoltaic Research
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    • v.4 no.2
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    • pp.68-79
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    • 2016
  • The power conversion efficiency of perovskite solar cells has remarkably increased from 3.81% to 22.1% in the past 6 years. Perovskite solar cells, which are based on the perovskite crystal structure, are fabricated using organic-inorganic hybrid materials. The advantages of these solar cells are their low cost and simple fabrication procedure. Also, they have a band gap of about 1.6 eV and effectively absorb light in the visible region. For the commercialization of perovskite solar cells in the field of photovoltaics, the issue of their long term stability cannot be overlooked. Although the development of perovskite solar cells is unprecedented, their main drawback is the degradation of the perovskite structure by moisture. This degradation is accelerated by exposure to UV light, temperature, and external bias. This paper reviews the aforesaid reasons for perovskite solar cell degradation. We also discuss the research directions that can lead to the development of perovskite solar cells with high stability.

Development of an Imaging-DOAS System for 2-D Remote Sensing of Atmospheric Gases (대기가스오염물질의 이차원 원격 모니터링을 위한 Imaging-DOAS 개발)

  • Lee, Han-Lim;Lee, Chul-Kyu;Jung, Jin-Sang;Park, Jeong-Eun;Kim, Young-Joon
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.2
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    • pp.150-157
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    • 2006
  • Spatially resolved remote identification and quantification of trace gases in the atmosphere is desirable in various fields of scientific research as well as in public security and industrial contexts. Environmental observations investigating causes, extent md consequences of air pollution are of fundamental interest. We present an Imaging-DOAS system, a ground based remote sensing instrument that allows spatially resolved mapping of atmospheric trace gases by a differential optical absorption spectroscopy(DOAS) with sun scattered light as the light source. A passive DOAS technique permits the identification and quantification of various gases, e.g., $NO_2,\;SO_2,\;and\;CH_2O$, from their differential absorption structures with high sensitivity. The Imaging-DOAS system consists of a scanning mirror, a focusing lens, a spectrometer, a 2-D CCD, ad the integral control software. An imaging spectrometer simultaneously acquires spectral information on the incident light in one spatial dimension(column) and sequentially scans the next spatial dimension with a motorized scanning mirror. The structure of the signal acquisition system is described in detail and the evaluation method is also briefly discussed. Applications of imaging of the $NO_2$ contents in the exhaust plumes from a power plant are presented.

Indentification of Specific Proteins synthesized During Somatic Embryogenesis of Rice (벼 callus로부터 재분화 과정에서 생성되는 특수한 단백질의 동정)

  • Won, Jae Hee;Choi, Hong Jib;Kim, Dal Ung
    • Current Research on Agriculture and Life Sciences
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    • v.9
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    • pp.95-102
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    • 1991
  • This study was conducted to investigate the physiological and biochemical elements relating embryogenisis. Also we examined the differences of protein in embryogenic callus and nonembryogenic callus by SDS-PAGE and two-dimensional gel electrophoresis. In this study, we have focused attention on the process of total proteins during sometic embryogenesis of rice. When compared the proteins of embryogenic callus and nonembryogenic callus, some different proteins were observed and serval proteins were increased in embryogenic callus. Some proteins were decreased or disappeared in embryogenic callus. Near 43kD protein band was only observed in embryogenic callus. The other bands were similar to each lines. The result of two-diimensional gel electrophoresis, E-callus specific proteins were observed. This Results may indicate that these proteins were associated with somatic embryogenesis.

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Design of spectrum spreading technique applied to DVB-S2

  • Kim, Pan-Soo;Chang, Dae-Ig;Lee, Ho-Jin
    • Journal of Satellite, Information and Communications
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    • v.2 no.2
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    • pp.22-28
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    • 2007
  • Spectrum spreading, in its general form, can be conceived as an artificial expansion of the signal bandwidth with respect to the minimum Nyquist band required to transmit the desired information. Spreading can be functional to several objectives, including resilience to interference and jammers and reduction of power spectral density levels. In the paper, signal spreading is manly used for increasing the received energy, thus satisfying link budget constraints, for terminals with low aperture antennas, without increasing the transmitted EIRP. As a matter of fact, in many mobile scenarios, even when MODCOD configurations with very low spectral efficiency (i.e. QPSK-1/4) in DVB-S2 standard, are used, the link budget cannot be closed. Spectrum spreading has been recently proposed as a technique to improve system performance without introducing additional MODCOD configurations under the constraint of fixed power spectrum density level at the transmitter side. To this aim, the design of spectrum spreading techniques shall keep into consideration requirements such as spectrum mask, physical layer performance, link budget, hardware reuse, robustness, complexity, and backward compliance with existing commercial equipments. The proposed implementation allows to fully reuse the standard DVB-S2 circuitry and is inserted as an 'inner layer' in the standard DVB-S2 chain.

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Analysis and Proposal of Communication System for Maritime HF Band Digital Data Exchange (해상 HF대역 디지털 데이터 교환을 위한 통신시스템 분석 및 제안)

  • Choi, Sung-Cheol;So, Ji-Eun;Park, Hyung-Chul
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.12
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    • pp.2249-2260
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    • 2017
  • IMO (International Maritime Organization) has been providing GMDSS (Global Maritime Distress and Safety System) and mandating to install distress and safety systems according to SOLAS. Digital-HF(High-Frequency) coast station communication system maintains interoperability between ship and coast station and digital data exchange in maritime mobile service by digitizing existing analog base voice communication. In this paper, we analyze ITU-R M. 1798-1 established by ITU for digital HF communications and propose Advanced annex2 and new Annex 5 to improve the problems of the existing Annex 2 and Annex 4. The proposed OFDM protocol basically adopts ARQ (Automatic Retransmission Request) which retransmits when an error occurs in a half-duplex manner between an information transmitting side (ISS) and an information receiving side (IRS) and we propose a digital HF communication system and its operational concept which is more reliable and superior than the existing ITU-R M. 1798 by implementing technical development on implementation and performance improvement.

Effects of Incompatibility on Protoplast Fusion between intra-and inter Species in Basidiomycete, Pleurotus spp. (느타리버섯의 불화합성(不和合性)이 종내(種內) 및 종간(種間) 원형질체(原形質體) 융합(融合)에 미치는 영향(影響))

  • Go, Seung-Joo;You, Chang-Hyun;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.137-144
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    • 1989
  • Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by Poly ethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed mutants.

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SOI CMOS Miniaturized Tunable Bandpass Filter with Two Transmission zeros for High Power Application (고 출력 응용을 위한 2개의 전송영점을 가지는 최소화된 SOI CMOS 가변 대역 통과 여파기)

  • Im, Dokyung;Im, Donggu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.1
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    • pp.174-179
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    • 2013
  • This paper presents a capacitor loaded tunable bandpass chip filter using multiple split ring resonators (MSRRs) with two transmission zeros. To obtain high selectivity and minimize the chip size, asymmetric feed lines are adopted to make a pair of transmission zeros located on each side of passband. Compared with conventional filters using cross-coupling or source-load coupling techniques, the proposed filter uses only two resonators to achieve high selectivity through a pair of transmission zeros. In order to optimize selectivity and sensitivity (insertion loss) of the filter, the effect of the position of asymmetric feed line on transmission zeros and insertion loss is analyzed. The SOI-CMOS switched capacitor composed of metal-insulator-metal (MIM) capacitor and stacked-FETs is loaded at outer rings of MSRRs to tune passband frequency and handle high power signal up to +30 dBm. By turning on or off the gate of the transistors, the passband frequency can be shifted from 4GH to 5GHz. The proposed on-chip filter is implemented in 0.18-${\mu}m$ SOI CMOS technology that makes it possible to integrate high-Q passive devices and stacked-FETs. The designed filter shows miniaturized size of only $4mm{\times}2mm$ (i.e., $0.177{\lambda}g{\times}0.088{\lambda}g$), where ${\lambda}g$ denotes the guided wave length of the $50{\Omega}$ microstrip line at center frequency. The measured insertion loss (S21)is about 5.1dB and 6.9dB at 5.4GHz and 4.5GHz, respectively. The designed filter shows out-of-band rejection greater than 20dB at 500MHz offset from center frequency.

A Study on the Design and Fabrication of Diplexer Using H-plane T-junction for KOREASAT-III Transponder (자계면 T-접합을 이용한 무궁화 III호 위성체용 다이플렉서의 설계 및 제작에 관한 연구)

  • 이용민;홍완표;신철재;강준길;나극환
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.4
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    • pp.582-593
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    • 1999
  • This paper presents the design and fabrication of the diplexer for a KOREASAT-III Ka-band satellite transponder. The transmission characteristics of the diplexer is analyzed by calculating the generalized scattering matrix using mode matching method. It is composed of 2 bandpass filters, coupled with H-plane T-junction having symmetrical inductive iris and E-plane metal insert structures. Compared with the size and weight of the Rx and Tx filter loaded with a transponders respectively, those of the diplexer can be effectively reduced. In a high power transmission, the variation of the filter characteristics is minimized by the scheme that irises are extended to the exterior of H-plane of the waveguide. This scheme needs no extra heat sinks for dissipating high power. The diplexer is designed to improve the simplification, durability and reliability by eliminating tuning screws, which have been used to compensate for the characteristics of fabricated filters. The bandpass filters of the diplexer show the insertion loss of less than 1.2 dB and the return loss in excess of 15 dB. The isolations of more than 65 dB have been achieved between Rx and Tx filter.

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Ultraviolet LEDs using n-ZnO:Ga/i-ZnO/p-GaN:Mg heterojunction (n-ZnO/i-ZnO/p-GaN:Mg 이종접합을 이용한 UV 발광 다이오드)

  • Han, W.S.;Kim, Y.Y.;Kong, B.H.;Cho, H.K.;Lee, J.H.;Kim, H.S.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.50-50
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    • 2008
  • ZnO has been extensively studied for optoelectronic applications such as blue and ultraviolet (UV) light emitters and detectors, because it has a wide band gap (3.37 eV) anda large exciton binding energy of ~60 meV over GaN (~26 meV). However, the fabrication of the light emitting devices using ZnO homojunctions is suffered from the lack of reproducibility of the p-type ZnO with high hall concentration and mobility. Thus, the ZnO-based p-n heterojunction light emitting diode (LED) using p-Si and p-GaN would be expected to exhibit stable device performance compared to the homojunction LED. The n-ZnO/p-GaN heterostructure is a good candidate for ZnO-based heterojunction LEDs because of their similar physical properties and the reproducibleavailability of p-type GaN. Especially, the reduced lattice mismatch (~1.8 %) and similar crystal structure result in the advantage of acquiring high performance LED devices with low defect density. However, the electroluminescence (EL) of the device using n-ZnO/p-GaN heterojunctions shows the blue and greenish emissions, which are attributed to the emission from the p-GaN and deep-level defects. In this work, the n-ZnO:Ga/p-GaN:Mg heterojunction light emitting diodes (LEDs) were fabricated at different growth temperatures and carrier concentrations in the n-type region. The effects of the growth temperature and carrier concentration on the electrical and emission properties were investigated. The I-V and the EL results showed that the device performance of the heterostructure LEDs, such as turn-on voltage and true ultraviolet emission, developed through the insertion of a thin intrinsic layer between n-ZnO:Ga and p-GaN:Mg. This observation was attributed to a lowering of the energy barriers for the supply of electrons and holes into intrinsic ZnO, and recombination in the intrinsic ZnO with the absence of deep level emission.

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