• Title/Summary/Keyword: Dynamic light scattering

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Design and Implementation of a Dynamic Instrumentation Framework based on Light-weight Dynamic Binary Translation (경량 동적 코드 변환 기법을 이용한 동적 인스트루멘테이션 기법 설계 및 구현)

  • Kim, Jeehong;Lee, Dongwoo;Kim, Inhyeok;Eom, Young Ik
    • Journal of KIISE
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    • v.41 no.11
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    • pp.892-899
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    • 2014
  • Dynamic binary instrumentation is a code insertion technique for debugging a program without scattering its execution flow, while the program is running. Most dynamic instrumentations are implemented using dynamic binary translation techniques. Existing studies translated program codes dynamically by parsing the machine code stream to intermediate representation (IR) and then applying compilation techniques for IRs. However, they have high overhead during translation, which is a major cause of difficulty in applying the dynamic binary translation technique to the program which requires high responsiveness. In this paper, we introduce a light-weight dynamic binary instrumentation framework based on a novel dynamic binary translation technique which has low overhead while translating the program code. In order to reduce the translation overhead, our approach adopts a tabular-based address translation and exploits a translation bypassing scheme, which stores the translated address of a frequently called library function in advance. It then accesses the translated address and executes function codes without code translation when calling the function. Our experiment results demonstrated that the proposed approach outperforms the prior dynamic binary translation techniques from 2% up to 65%.

Spontaneous Nanoparticle Formation From a Fluorescent Nucleoside Analogue

  • Bang, Eun-Kyoung;Moon, Do-Hyun;Kim, Byeang-Hyean
    • Bulletin of the Korean Chemical Society
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    • v.32 no.spc8
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    • pp.2906-2910
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    • 2011
  • A fluorescent nucleoside analogue, $^AC$, featuring two non-complementary nucleobases linked through an ethynyl group, was synthesized. The extended ${\pi}$-conjugation imparts $^AC$ with red-shifted absorbance (relative to adenine and cytosine) and pale-blue fluorescence. It spontaneously forms nanoparticles, which exhibit considerably enhanced fluorescence, without the help of any additional stabilizing agent. The DMSO/water ratio was an important factor influencing the construction of the NPs. X-ray crystallography confirmed the structure of $^AC$; dynamic light scattering and scanning electron microscopy confirmed the existence of the nanoparticles.

Characterization of Linear Polymer-Dendrimer Block Copolymer/Plasmid DNA Complexes: Formation of Core-shell Type Nanoparticles with DNA and Application to Gene Delivery in Vitro

  • Choi, Joon-Sig;Choi, Young-Hun;Park, Jong-Sang
    • Bulletin of the Korean Chemical Society
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    • v.25 no.7
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    • pp.1025-1030
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    • 2004
  • A hybrid linear polymer-dendrimer block copolymer, poly(ethylene glycol)-block-poly(L-lysine) dendrimer, was synthesized and introduced to form polyionic complexes with DNA. The copolymer formed core-shell type nanoparticles with plasmid DNA. From dynamic light scattering experiments, the mean diameter of the polyplexes was observed to be 154.4 nm. The complex showed much increased water solubility compared to poly(L-lysine). The plasmid DNA in polyplexes was efficiently protected from the enzymatic digestion of DNase I. The cytotoxicity and transfection efficiency for 293 cells was measured in comparison with poly(Llysine).

Microbial Production and Characterization of Superparamagnetic Magnetite Nanoparticles by Shewanella sp. HN-41

  • Lee, Ji-Hoon;Roh, Yul;Hur, Hor-Gil
    • Journal of Microbiology and Biotechnology
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    • v.18 no.9
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    • pp.1572-1577
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    • 2008
  • A facultative dissimilatory metal-reducing bacterium, Shewanella sp. strain HN-41, was used to produce magnetite nanoparticles from a precursor, poorly crystalline iron-oxyhydroxide akaganeite ($\beta$-FeOOH), by reducing Fe(III). The diameter of the biogenic magnetite nanoparticles ranged from 26 nm to 38 nm, characterized by dynamic light scattering spectrophotometry. The magnetite nanoparticles consisted of mostly uniformly shaped spheres, which were identified by electron microscopy. The magnetometry revealed the superparamagnetic property of the magnetic nanoparticles. The atomic structure of the biogenic magnetite, which was determined by extended X-ray absorption fine structure spectroscopic analysis, showed similar atomic structural parameters, such as atomic distances and coordinations, to typical magnetite mineral.

Three-Dimensional Self-Assembly of Gold Nanoparticles Using a Virus Scaffold

  • Kang, Aeyeon;Lee, Young-Mi;Kang, Hyo Jin;Chung, Sang Jeon;Yun, Wan Soo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.651-651
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    • 2013
  • Templated strategy is a very powerful tool for creating multi-dimensional self assembly of nanomaterials. Since viral protein cages have a uniform size with a well-defined structure, they can serve as an excellent template for the formation of a three-dimensional self-assembly of synthetic nanoparticles. In this study, we have examined the feasibility of the 3D self-assembly of gold nanoparticles of various sizes using a brome mosaic virus (BMV) capsid with cysteine groups expressed on its surface as a scaffold for the assembly. It was found that the three-dimensional clusters of gold nanoparticles with a designed structure were attainable by this approach, which was verified by transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis.

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Thermodynamics of the binding of Substance P to lipid membranes

  • Lee, Woong Hyoung;Kim, Chul
    • Analytical Science and Technology
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    • v.30 no.2
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    • pp.89-95
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    • 2017
  • The thermodynamic functions for the binding of the peptide Substance P (SP) on the surface of lipid vesicles made of various types of lipids were obtained by using isothermal titration calorimetry. The reaction enthalpies measured from the experiments were -0.11 to $-4.5kcal\;mol^{-1}$. The sizes of the lipid vesicles were measured with dynamic light scattering instrument in order to get the correlation between the reaction enthalpies and the vesicle sizes. The bindings of SP on the lipid vesicles with diameter of 37 to 108 nm were classified into the enthalpy-driven reaction or the entropy-driven reaction according to the size of the lipid vesicles. For the enthalpy-driven binding reaction, the significance of the electrostatic interactions between SP and lipid molecules was affirmed from the experimental results of the DMPC/DMPG/DMPH and DMPC/DMPS/DMPH vesicles as well as the importance of the hydrophobic interactions between hydrophobic groups of SP and lipid molecules.

Chemical synthesis of processable conducting polyaniline derivative with free amine functional groups

  • Kar, Pradip
    • Advances in materials Research
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    • v.3 no.2
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    • pp.117-128
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    • 2014
  • Processable conducting polyaniline derivative with free amine functional groups was successfully synthesized from the monomer o-phenylenediamine in aqueous hydrochloric acid medium using ammonium persulfate as an oxidative initiator. The synthesized poly(o-phenylenediamine) (PoPD) in critical condition was found to be completely soluble in common organic solvents like dimethyl sulfoxide, N,N-dimethyl formamide etc. From the intrinsic viscosity measurement, the optimum condition for the polymerization was established. The polymer was characterized by ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy, proton magnetic resonance spectroscopy ($^1HNMR$) and thermogravimetric (TGA) analyses. The weight average molecular weights of the synthesized polymers were determined by the dynamic light scattering (DLS) method. From the spectroscopic analysis the structure was found to resemble that of polyaniline derivative with free amine functional groups attached to ortho/meta position in the phenyl ring. However, very little ladder unit was also present with in the polymer chain. The moderate thermal stability of the synthesized polymer could be found from the TGA analysis. The average DC conductivity of $2.8{\times}10^{-4}S/cm$ was observed for the synthesized polymer pellet after doping with hydrochloric acid.

Synthesis of gold nanoparticles using Coffea Arabica fruit extract

  • Bogireddy, Naveen Kumar Reddy;Gomez, L. Martinez;Osorio-Roman, I.;Agarwal, V.
    • Advances in nano research
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    • v.5 no.3
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    • pp.253-260
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    • 2017
  • We report a simple eco-friendly process for the synthesis of gold nanoparticles (AuNPs) using aqueous extract from Coffea Arabica fruit. The formation of AuNPs was confirmed using absorption spectroscopy and scanning electron microscopy images. FT-IR analysis demonstrates the major functional groups present in Coffee Arabica fruit extract before and after synthesizing AuNPs. The Face Center Cubic (FCC) polycrystalline nature of these particles was identified by X-Ray diffraction (XRD) analysis. Taking into account the contribution of the biomass surrounding the AuNPs, dynamic light scattering (DLS) results revealed an average particle size of ~59 nm.

Synthesis and Thermo-mechanical Property of Multi-walled Carbon Nanotubes/Poly(methyl methacrylate-co-butyl acrylate) Nanocomposites Prepared Using Emulsion Polymerizations in the Presence of Amphiphilic Random Terpolymer

  • Chang, Woo-Hyuck;Ki, Ho-Seong;Cheong, In-Woo
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.289-289
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    • 2006
  • The carboxylated MWNTs were successfully prepared by conventional acid treatment, and their structures were confirmed by FT-IR, Raman and TEM analysis. The water-dispersibility of the surface modified WNTs were good. The COOH-MWNT will show better stability during the emulsion polymerization as compared with Pristine MWNT. In-situ emulsion polymerizations of methyl methacrylate N(MMA) and n-butyl acrylate (BA) were carried out. Aggregate size and dispersion stability of the CNTs in water phase were measured using dynamic light scattering, turbidity, UV-visible spectrophotometer, and electron microscope. In addition, thermo-mechanical properties of MWNT/polymer nanocomposites were investigated.

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Fabrication of nanoaggregates of triple hydrophilic block copolymers by binding of ionic surfactants

  • Khanal, Anil;Yusa, Shin-Ichi;Nakashima, Kenichi
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.302-302
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    • 2006
  • Nanoaggregates of triple hydrophilic block copolymers comprised of poly(ethylene oxide), poly(sodium 2-acrylamido)-2-methylpropanesulfonate), and poly(methacrylic acid) (PEO-PAMPS-PMAA) and the cationic surfactant, dodecyltrimethylammonium chloride (DTAC) have been fabricated. The formation of $^{\circ}^{\circ}$the nanoaggregates is based on electrostatic interaction of sulfonate and carboxylate groups of PAMPS and PMAA blocks with the cationic surfactant, which results in insolubilization of these blocks. The formation of micelle is observed by dynamic light scattering measurements. Binding of DTAC to the anionic blocks of PEO-PAMPS-PMAA is confirmed by electrophoresis measurements.

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