• Journal of Animal Science and Technology
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• 제66권4호
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• pp.846-858
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• 2024

In a duck cage, ducks are placed in various states. In particular, if a duck is overturned and falls or dies, it will adversely affect the growing environment. In order to prevent the foregoing, it was necessary to continuously manage the cage for duck growth. This study proposes a method using an object detection algorithm to improve the foregoing. Object detection refers to the work to perform classification and localization of all objects present in the image when an input image is given. To use an object detection algorithm in a duck cage, data to be used for learning should be made and the data should be augmented to secure enough data to learn from. In addition, the time required for object detection and the accuracy of object detection are important. The study collected, processed, and augmented image data for a total of two years in 2021 and 2022 from the duck cage. Based on the objects that must be detected, the data collected as such were divided at a ratio of 9 : 1, and learning and verification were performed. The final results were visually confirmed using images different from the images used for learning. The proposed method is expected to be used for minimizing human resources in the growing process in duck cages and making the duck cages into smart farms.

• 한국축산식품학회지
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• 제33권5호
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• pp.655-663
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• 2013

본 연구에서는 Salmonella invA 유전자를 마커로 Salmonella 특이 LAMP primer를 제작하였고, inclusivity 및 exclusivity 실험 결과는 각각 100%로 관찰되었다. 순수 배양된 Salmonella 희석액에서의 검출한계는 18.17분에서 $3.2{\times}10^3$ CFU/mL ($R^2$ = 0.9446)으로 관찰되어 신속 간단하고 민감도가 높아 Salmonella 검출을 위한 방법으로 효과적일 뿐만 아니라 간접적인 정량기법으로서도 활용 가능함을 알 수 있었다. 1차 증균 배지인 BPW에 S. Enteritidis 접종 후 0시간 및 12시간 배양 후 LAMP의 검출한계는 각각 $3.2{\times}10^3$ CFU/mL 및 $3.2{\times}10^0$ CFU/mL으로 관찰되었고, 오리도체 시료가 포함된 BPW에 S. Enteritidis 접종 후 0시간 및 12시간 증균액에서의 LAMP 검출한계는 각각 $3.2{\times}10^3$ CFU/mL 및 $3.2{\times}10^0$ CFU/mL으로 관찰되어 PCR과 비교하였을 때 오리도체시료 성분에 의한 영향이 비교적 낮았음을 알 수 있었다. 또한, 실제 도압장에서 채취한 오리도체시료를 BPW에 6시간 배양한 1차 증균액에 개발한 LAMP기법을 적용한 결과, 96%의 민감도 및 84%의 특이도를 보여 오리도체에서의 Salmonella 신속 스크리닝 기법으로서 효과적으로 활용될 수 있음을 알 수 있었다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2014년도 임시총회 및 하계학술연구발표회
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• pp.105-105
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• 2014

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2008년도 Asian Magnetics Conference
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• pp.208.1-208.1
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• 2008

• 한국동물위생학회지
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• 제34권4호
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• 한국동물위생학회지
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• 제33권4호
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• pp.341-344
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• 2010

Poultry and poultry products have been implicated as a major source of Salmonella infection in human, and infection due to Salmonella serotypes continue to be a major health problem. The presence of two virulence genes, invA and spvC, in 34 Salmonella isolates obtained from duck farms was investigated. All isolates contained the invA gene, and spvC gene was found in 20 (58.8%) of 34 Salmonella isolates : S. Typhimurium (n=8), S. Fyris (n=5), S. Enteritidis (n=3), S. Typhimurium var. copenhagen (n=1), S. Haardt (n=1) and S. Mbandaka (n=1). This study showed the presence of the spvC gene was widely distributed in between different Salmonella enterica isolates.

• 한국동물위생학회지
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• 제16권2호
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• pp.91-96
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• 1993

In order to monitor the parasites, fecal samples were taken from turkey (n=157), helmeted guineafowl(n=149), pheasant(n=190) and duck(n=190) in Chonbuk province. The identification of the parasites were determined by the fecal examination using the fluatation method and microscopical examination. The results were obtained as follows；1. The detection rate oi the parasites from 4 species of poutry was 47.2%(n=324 heads) out of 686 heads. 2. The identification rate was 85.9% in helmeted guineafowl, 63.2% in pheasant, 44.6% in turkey and 3.2% in duck, in order. 3. The mixed infection rate such as single, double, triple and quadrupl was 25.4%(174 heads), 14.1%(97 heads), 7.3%(50 heads) and 0.4%(3 heads), respectively. 4. The parasites isolated were identified as Capillaria spp. in 225 heads, Eimeria spp in 169 heads, Heterakis gallinarum in 116 heads, Ascaridia galli in 16 heads, Hymenolepis spp. in 3 heads and Strongyloides avium in 1 head, in order.

• 산업경영시스템학회지
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• 제42권2호
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• pp.28-35
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• 2019

Since guided missiles with the characteristics of the one-shot system remain stored throughout their entire life cycle, it is important to maintain their storage reliability until the launch. As part of maintaining storage reliability, period of preventive test is set up to perform preventive periodic test, in this case failure detection rate has a great effect on setting up period of preventive test to maintain storage reliability. The proposed method utilizes failure rate predicted by the software on the basis of MIL-HDBK-217F and failure mode analyzed through FMEA (Failure Mode and Effect Analysis) using data generated from the actual field. The failure detection rate of using the proposed method is applied to set periodic test of the actual guided missile. The proposed method in this paper has advantages in accuracy and objectivity because it utilizes a large amount of data generated in the actual field.

• 한국동물위생학회지
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• 제35권3호
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• pp.215-222
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• 2012

Species-specific $TaqMan^{(R)}$ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated ($121^{\circ}C$/5 min) meat samples. In conclusion, it can be suggested that the $TaqMan^{(R)}$ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.

• Journal of Magnetics
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• 제14권2호
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• pp.97-100
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• 2009

A new eddy current testing probe was developed to separate the eddy current signal distortion caused by permeability variation clusters and ordinary defects created in steam generator tubes. Signal processing circuits were inserted into the probe to increase the signal-to-noise ratio and allow digital signal transmission. The new probe could measure and separate the magnetic phases created in the steam generator tubes in the operating environment of a nuclear power plant. Furthermore, the new eddy current testing probe can measure the defects in steam generator tubes as rapidly as a bobbin probe with enhanced testing speed and reliability of defect detection.